http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Phenothiazines의 광독성에 대한 in vitro 실험법의 비교 연구
김종예,김현진,김봉희 한국환경독성학회 2000 환경독성보건학회지 Vol.15 No.1
A few in vitro methods were developed to compare the result on the phototoxicity of phenothiazines. By the MTT assay, the Candida test, and the RBC photohemolysis, the phototoxicities of UVA and UVB irradiation were measured. This paper presents the comparisons of methods which are effective to measure the phototoxicities of the chemicals causing phototoxicity and photoallergy. The tested chemicals of phenothiazines include Chlorpromazine, Promethazine, Perphenazine, Chlorprothixene, Trifluoperazine, and Thioridazine. Each chemical represented variable results according to the test methods. MTT assay shows the most sensitive method.
Phenothiazines의 광독성에 대한 in vitro 실험법에 따른 Ascorbic acid의 영향
김종예,김현진,김봉희 충남대학교 약학대학 의약품개발연구소 2002 藥學論文集 Vol.17 No.-
The effects of ascorbic acid a well-known as antioxidant agent, on the phototoxicity caused by phenothiazines were studied using three in vitro methods - MTT assay, the Candida test, and the RBC photohemolysis. The tested phenothiazines include Chlorpromazine, Promethazine, Perphenazine, Chlorprothixene, Trifluoperazine, and Thioridazine and UVA 1.5 J/㎠ were irradiated. In results, ascorbic acid was effective to reduce the phototoxicities and to decrease the production of toxic photoproducts in every method used.
UVB조사에 의한 Phenothiazine의 광독성에 미치는 Ascorbic Acid의 영향
임연일,김종예,김봉희 충남대학교 약학대학 의약품개발연구소 1998 藥學論文集 Vol.14 No.-
광독성 및 광알러지를 일으키는 물질인 phenothiazine계 약물중 chlorpromazine(CPZ), perphenazine(PPZ), trifluoperazine(TFZ), promethazine(PMZ)을 택하여 적혈구에서의 광용혈현상을 Kahn 등의 방법에 의해 UVB(UV irradiation RMX-3W, 1.5 J/㎠)을 조사하여 약물 농도별로 측정한 결과 CPZ, PPZ은 UVB 조사에 의해 약물농도에 따라 현저히 적혈구 용혈현상이 증가 되었으며 이는 ascorbic acid에 의해 유의성있게 감소되었고, 각 약물의 광독성 물질 생성 여부와 이에 의한 용혈독성을 조사해보니 CPZ, PMZ에서 관찰되었고 ascorbic acid에 의해 그 현상이 감소되었다. The purpose of this study is to examine the phototoxicity of four phenothiazine derivatives such as chlorpromazine, perphenazine, trifluorperazine and promethazine, and to investigate the effects of ascorbic acid on their phototoxicity by UVB irradiation. Effects of the test compounds on RBCs were monitored with a spectrophotometer by the method of Kahn et al. The extent of photohemolysis by tested phenothiazine derivatives were increased with their concentration and toxic photoproducts were formed by chlorpromazine and promethazine with preirradiated UVB. Photohemolysis postirradiated chlorpromazine and perphenazine and preirradiated promethazine were decreased with the use of ascorbic acid significantly.
UVB 조사에 의한 Phenothiazine 의 광독성에 미치는 Ascorbic Acid 의 영향
임연일,김종예,김봉희 한국식품위생안전성학회 1998 한국식품위생안전성학회지 Vol.13 No.3
The purpose of this study is to examine the phototoxicity of four phenothiazine derivatives such as chlorpromazine, perphenazine, trifluoroperazine and promethazine, and to investigate the effects of ascorbic acid on their phototoxicity by LTVB irradiation. Effects of the test compounds on RBCs were monitored with a spectrophotometer by the method of Kahn et al. The extent of photohemolysis by tested phenothiazine derivatives were increased with their concentration and toxic photoproducts were formed by chlorpromazine and promethazine with preirradiated UVB. Photohemolysis postirradiated chlorpromazine and perphenazine and preirradiated promethazine were decreased with the use of ascorbic acid significantly.
최진혁,신혜영,김종예 한국식품과학회 2024 한국식품과학회지 Vol.56 No.1
In this study, the effect of the calcium content on the viscosity of alginate was evaluated to replace carbomer, commonly used as a synthetic thickening agent in the cosmetic industry. The apparent viscosity of alginate solutions (0.1- 0.5%) was assessed, depending on the level of added calcium salt. The apparent viscosity of the alginate hydrogel increased with an increasing calcium concentration. For a 0.4% alginate solution with a 0.052% calcium concentration, a viscosity of 25,480 cp was noted. However, when the ratio of alginate to calcium exceeded 13%, alginate aggregation occurred, leading to a reduction in the apparent viscosity. For the optimized alginate hydrogel, its viscosity remained stable at pH 5 or higher, but the apparent viscosity decreased under acidic conditions (pH less than 4). The apparent viscosity of the optimized alginate hydrogel gradually decreased with higher temperatures. Accordingly, the addition of NaCl significantly reduced the apparent viscosity of the optimized alginate hydrogel.
브로콜리 추출물을 이용한 레드 비트 색소의 저장 안정성 향상 연구
김종훈,강지연,고은별,김종예 한국식품과학회 2019 한국식품과학회지 Vol.51 No.6
The effect of broccoli extracts on the storage stability of red beet extracts under various conditions (temperature, light condition, and pH) was analyzed. Regardless of the pH and light conditions, the absorbance of the red beet pigment at 537 nm was relatively stable (less than 10% reduction) at 4°C for up to 5 days. The absorbance of the control extracts was rapidly reduced during storage at an ambient temperature in the dark, but the degree of reduction was relatively low at pH 3.8. As a positive control, the addition of vitamin C to the control extracts slightly inhibited the reduction (40% reduction), but the degree of the inhibition was much higher (15% reduction) with the addition of broccoli extracts (in 70% ethanol). Among all the samples, the addition of the broccoli floret extracts using 70% ethanol was the most effective method for the enhancement of the storage stability of red beet pigment at an ambient temperature in the dark.
이태경,허기현,최정윤,반충진,김종예,양희,박정한,이기원,김종훈 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.9
The effect of nanoemulsions on the stability and bioavailability of sulforaphene (SFEN) in radish seed extract (RSE) was investigated. Four types of oil were used as lipid ingredients of the nanoemulsions: soybean, high oleic acid sunflower, coconut, and hydrogenated palm oils. SFEN in RSE nanoemulsions showed greater stability to temperature, acid, and alkaline conditions than SFEN in RSE suspended in water (RSE-S). Particularly under alkaline conditions, the half-life of SFEN in the nanoemulsion with high oleic sunflower oil (RSE-HOSO) was 8 times longer than that of RSE-S. Furthermore, in the pharmacokinetics study, it was observed that AUC0–8 increased and oral clearance (CL/F) decreased significantly in rats orally administered RSE-HOSO compared with RSE-S (p < 0.05). This study indicates that the type of oil used in nanoemulsions affects the stability and bioavailability of SFEN in RSE. These results may provide a guideline for the development of functional foods containing RSE.
조명래,고석범,김재민,이옥환,이대원,김종예 한국응용생명화학회 2016 Applied Biological Chemistry (Appl Biol Chem) Vol.59 No.3
This study investigated the influence of extraction conditions on antioxidant activities and catechin content from bark of Ulmus pumila. The U. pumila was extracted using various concentrations of ethanol (30, 50, 80, and 99 %) and different extraction times (1, 2, and 3 h) at 60 °C. The ethanol extracts were evaluated for their antioxidant activities [1,1-diphenyl-1-picrylhydrazyl, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activities and reducing power], total phenolic (TP), and proanthocyanidin (TA) contents. In addition, the antioxidant activities were correlated with TP and TA contents, and the catechin content was determined using high-performance liquid chromatography. The 99 % EtOH for 3-h extracts exhibited the highest antioxidant abilities with the highest phenolic and proanthocyanidin contents. TP content showed little correlation (R2 = 0.31–0.61) with their antioxidant activities; however, a significant correlation (R2 = 0.65–0.91) was observed with proanthocyanidin content. The catechin content of the 99 % EtOH for 3-h extracts was greater than that of other EtOH extracts. Therefore, these results indicate that the antioxidant capacity of EtOH extracts from bark of U. pumila may be due to catechin, and the optimal extraction condition was 99 % EtOH for 3 h.