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종설논문 : 의사-제약회사 상호관계가 연구에 미치는 영향
김승후 ( Seong Who Kim ),홍정화 ( Jeong Hwa Hong ),김옥주 ( Ock Joo Kim ) 한국의료윤리학회 2011 한국의료윤리학회지 Vol.14 No.3
This article examines the influence of physician-pharmaceutical industry interaction upon medical research. We analyze some of the problems arising from pharmaceutical companies` sponsorship of clinical trials, such as biased results caused by multiple trials with predictable outcomes and publication biases. Furthermore, we propose specific measures to address and overcome these problems.
사람 혈청 Vitronectin의 정제 및 항 Vitronectin 다클론 항체의 생산
장윤혜,김백남,이성순,문경,김승후,이재담 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.2
Background: Vitronectin is one of major cell- adhesive glycoprotein in mammalian serum and plasma ; the other is fibronectin. It is a mixture of 65 and 75kDa protein in plasma which promotes spreading of a variety of cultured cells ,inhibits the cytotoxicity of membrane attacks complex C5b-7 and modulates thrombin-antithrombin III activity. Human plasma and serum contain 10-40mg of vitronectin per 100ml,but only a few mg or less of vitronectin can be isolated with a 0.5-20% recovery efficiency through very long processes or with expensive,commercially available monoclonal antibody. In this study, we purified the vitronectin from human plasma and produced anti-vitronectin polyclonal antibody using purified vitronectin. Methods: Vitronectin was purified from the human plasma by heparin-sepharose column and its efficacy was measured by cell spreading assay. Anti-vitronectin polyclonal antibody produced and confirmed by ELISA and immunoblotting. Results: This procedures produced about 0.8mg vitronectin from 100ml human plasma within 2 days. 1) Purified vitronectin promoted spreading of HepG2 hepatoma cells on substrates with a half maximal activity at 0.lug/ml. 2) In SDS-PAGE analysis of purified protein, 2 bands were found and their molecular weights were 75kDa and 65kDa,respectively. 3) The immunoblotting assay showed that the bands of molecular were same site as SDSPAGE analysis. Conclusion: Simple,rapid purified vitronectin by heparin-sepharose column and anti-vitronectin antibody may facilitate the elucidation of vitronectin function and action mechanism in human body.
사람 내피세포에서 염증 전구사이토카인이 VCAM - 1의 발현에 미치는 영향
조영주,박수길,이재담,황온유,문희범,장윤혜,김승후,홍혜남 대한알레르기학회 1999 천식 및 알레르기 Vol.19 No.2
Background: The expression of adhesion molecules contribute to development of systemic diseases. Vascular cell adhesion molecule-l(VCAM-1) is an endothelial cell membrane glycoprotein that has been implicated in leukocyte/endothelial cell interactions in inflammation. Ojective: The aim of this study was to characterize the surface expression and regulation of VCAM-1 on two different endothelial cells. Methods: We examined the effects of the expression of VCAM-1 in two different endothelial cells, isolated from human umbilical cords and human glomerulus. Expression of VCAM-1 was measured by enzyme-linked immunosorbent assay(ELISA) and flow cytometry. Results: In human umbilical cord endothelial cells(HUVECs), both interleukin-lβ(IL-lβ) and tumor necrosis factor-α(TNF-α) increased VCAM-1 expression. VCAM-1 expression increased by TNF-αwas higher than that increased by IL-lβ. In human glomerular endothelial cells(HGECs), IL-lβand TNF-αmarkedly increased VCAM-1 expression. Conclusion. The regulation of VCAM-1 appears to be somewhat different in HGECs compared with HUVECs. These differences between the responsiveness of the two cells may possibly indicate inherent differences in endothelial cell derived from different vascular beds.