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노형진 ( Heyong Jin Roh ),임윤진 ( Yun-jin Lim ),김아란 ( Ahran Kim ),김남은 ( Nam Eun Kim ),김영재 ( Youngjae Kim ),박노백 ( Noh Back Park ),황지연 ( Jee-youn Hwang ),권문경 ( Mun-gyeong Kwon ),김도형 ( Do-hyung Kim ) 한국수산과학회(구 한국수산학회) 2018 한국수산과학회지 Vol.51 No.4
We examined correlations of the density of fish farms with the distributions of indicator bacteria (Escherichia coli, fecal streptococci) and a bacterial fish pathogen (Streptococcus parauberis) off the coastline of Jeju Island. Seawater samples were collected at four coastal sites on the Island [Aewol (control), Gujwa, Pyoseon and Daejeong] in June, August and October 2016. The indicator bacteria were generally more frequently isolated from samples taken in August when water temperatures and human activities on nearby beaches were highest. Although fish farms were least common at Daejeong, the numbers of isolated fecal indicator bacteria were highest in the seawater and effluent water collected from this site. Hence, fish farms were not likely major contributors of indicator bacteria at Daejeong. We found discrepancies between the isolated bacterial counts and the predicted bacterial copy numbers deduced from our qPCR results, indicating that this pathogen may exist in a viable but non-culturable (VBNC) state in seawater. Thus, livestock wastewater and chemical fertilizer loading off Jeju Island may negatively impact seawater quality more than the effluent released from fish farms does.
무지개송어(Onchorhynchus mykiss) 위팽창증후군의 잠재적 원인체의 분자유전학적 동정
노형진,김도형,Roh, Heyong Jin,Kim, Do-Hyung 한국수산과학회 2017 한국수산과학회지 Vol.50 No.5
A rainbow trout Onchorhynchus mykiss farm located in Gangwon province, South Korea, experienced approximately 10% mortality in June 2017. Most diseased fish had a markedly distended, gas-filled stomach, and exhibited abnormal behavior at the water surface. In this study, we attempted to identify the cause of stomach distension syndrome in those rainbow trout. The stomach of most of the affected fish were full of unidentified gases and some exudate, and yeast was isolated from the stomach mucosa. Pure cultures of yeast were identified using a multilocus sequence typing scheme based on 18S rRNA, internal transcribed spacers, large subunit rRNA, and the gene encoding the largest subunit of RNA polymerase II (RPB1). The RPB1 gene sequences were compared with those of related species available in a database. The yeast was identified as Scheffersomyces coipomoensis (Candida coipomoensis) based on sequence analyses. This is the first study to reveal that Sch. coipomoensis is a potential causative agent of stomach distension syndrome in farmed rainbow trout. Our results will be helpful for future related studies, and indicate that farmers and stakeholders should observe this emerging disease closely.
양식 넙치에서 Parvicapsula anisocaudata의 감염 사례에 대한 보고
김남은,김아란,노형진,강경식,김도형,Kim, Nam Eun,Kim, Ahran,Roh, Heyong Jin,Gang, Kyoung Sik,Kim, Do-Hyung 한국어병학회 2018 한국어병학회지 Vol.31 No.2
Parvicapsula anisocaudata, a myxosporean parasite, is presumably one of causative agents of emaciation in olive flounder Paralichthys olivaceus in Korea. In this study, we report a case of unusual abdominal distension due to exceptionally enlarged liver in farmed olive flounder. For the identification of the causative agent, bacteria and nucleic acids of virus that are possibly present were attempted to isolate from internal organs of five fish sampled from a fish farm in Jeju. Although a few bacterial colonies were isolated from some samples, there was no evidence that fish were primarily affected by virus and/or bacteria. From histopathological analysis, myxosporean were found in almost all internal organs, particularly in the stomach. The causative agent was identified as P. anisocaudata by sequencing a part of small subunit rRNA. This study contains a very unusual case of olive flounder heavily and systemically infected with P. anisocaudata, showing excessively enlarged liver with a small amount of ascitic fluid.
송진호(Jinho Song),지홍섭(Hongsub Jee),문대한(Daehan Moon),김도형(Do-Heyong Kim),양오봉(O-Bong Yang),정채환(Chaehwan Jeong) 한국태양광발전학회 2019 Current Photovoltaic Research Vol.7 No.2
The high density module (HDM) has advantages for its larger active area and smaller current density. This new way of making a photovoltaic (PV) module method has benefit for increasing module power with the same installed area. Because HDM consisted with serially connected PV strings, loss of strings during the fabrication process can increase the overall production cost.1-2 This study investigates the rework conditions of the shingled strings with electrically conductive adhesives (ECA). By heating the electrically connected area of a fabricated string, cured area become soft and a string can be detached for the rework process. After rework process, a refabricated string showed 5~10% increased output power compared to before rework process and reached to the 90~95% output power compare to the undamaged strings.