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서부 경남지역 딸기 농장에서의 Staphylococcus aureus의 분리와 Staphylococcal enterotoxin a, b, c gene 검색
김세리(Se-Ri Kim),심원보(Won-Bo Shim),김지훈(Ji-Hun Kim),황승재(Seung-Jae Hwang),박선자(Seon-Ja Park),하상도(Sang-Do Ha),김근성(Keun-Sung Kim),이규호(Kyu-Ho Lee),김민곤(Min-Gon Kim),김광엽(Kwang-Yup Kim),김철호(Cheol-Ho Lim),정덕화(Duck 한국식품과학회 2005 한국식품과학회지 Vol.37 No.2
지역커뮤니티 시설로서 종합교육관 건축디자인 과정에 관한 연구
김근성(Kim, Keun-Sung) 한국주거환경학회 2014 주거환경(한국주거환경학회논문집) Vol.12 No.2
As the society experiences modernization, most of original central business district is deteriorating due to relocation of residential and commercial functions out to newly emerging development sites in the surroundings. Educational environment requires the social sense of crisis as well as changed role of universities, as the current population for admission to universities will diminish by 160,000 headcounts in 2014. If educational facilities are located in original central business district and plan, utilize facilities in linkage with adjacent regions, and then a small budget could achieve a lot of effects. The university facilities located in the original central business district have the merit of inducing various cultural spaces, simple planning as educational and research facilities is abstained, in order to plan regional community facilities applying facilities complexation concept in consideration of the demands of regional residents. The approach to architecture design process shall not the design ordering with abstract design direction, but clearly defining the purpose of architecture by planned design and concretely computing the category and size of facilities to be attracted to the site, so as to be used as the guidance material or design and construction ordering. Such architecture design process will be proceed in feedback form and enhances the understanding by the designer, so as to bring the effect of preventing errors during intermediate process and saving budget from unnecessary wastes.
김근성(Kim, Keun-Sung) 한국주거환경학회 2011 주거환경(한국주거환경학회논문집) Vol.9 No.1
This study is going to approach a method of healing the industrialization and personalization phenomena, which contribute to the absence of communication owing to the collapse of a community, from an architectural perspective, and to offer a convergence cultural space through the application of architect and art. The study aims to propose a cultural facility in a local community that can be created as a social communication facility, and to plan a comfortable facility as a field of communications among users, where many residents converse, take a rest, and talk about their difficulties also as being a facility like a village reception room instead of an authoritative facility which is difficult to approach. The designation of the most efficient cultural facility in the creation of local community may be determined by local characteristics; however, in order to prepare universal criteria, it is reasonable that a field should be accessible by people of all ages and can be applied to local contents, smoothly. As to the current representative artists of Jinju, they are discovered to be concentrated in the field of fine art, and there is no exhibition space where people are able to experience the artworks of those artists; thus, it is supposed to be necessary to have an exhibition space for the artworks of local artists as there is no exhibition space to experience their works. Regarding the design concept, the study made a plan in harmony with school facilities in connection with local social, cultural facilities and the urban green networks, and approached designing in a manner to preserve the natural environment of the existing land. Concerning exhibition facilities to be planned on the school site, the study designed them in a way to play a role in the local community as a minimum architectural space in liaison with circumferential functions so that they can be beneficial in terms of repair and maintenance. For the planning of gardening, the study approaches it in a way that it would least damage the existing scenaries and trees and shrubs.
김근성(Kim, keun-sung) 한국주거환경학회 2012 주거환경(한국주거환경학회논문집) Vol.10 No.3
The collapse of our rural community started to appear from the industrialization in the 1960s. The unbalanced social phenomenon, in which the population and the capital were crammed to the cities, was a dark shadow of our society where industrialization was implemented intensively. This has been one of the social assignments that should be resolved. This study was aimed to solve an axis of social imbalance through the rural village activity. The target area, Yangpo Village, has suffered from a decline due to a continuous decrease in population and economic power, thus searching for ways to solve the problems through systematic support plans. The activation methods for Yangpo Village could be divided into a plan for population inflow, a plan for spatial environment improvements, and a plan for ensuring its community. First, the plan for population inflow was related to helping marriage immigrants, those returning to farming and returning to village migrants settle down in the village stably by appropriate supports. This plan could provide housing by using empty houses, income opportunities, and cultural infusion programs. Second, the plan for spatial environment improvement was about the improvement of village space structures and dwelling facilities. It involved in the transfer of a road that penetrated the village and the division of activities of small-scale living sphere by region. It attempted to distribute public facilities appropriately, thus enhancing the accessibility and usability of the residents of village. Third, the plan for ensuring the community was related to smooth interchange among the residents settling down in the village. It was aimed at improving the connectivity through the crossing of living traffic lines by introducing the concept of facility sharing using public facilities.
자가숙성발효 후 흑마늘의 S-Allyl-L-cystein, Diallyl Disulfide 및 Total Amino Acids 분석
김문수(Mun-Su Kim),김민주(Min-Ju Kim),방우석(Woo-Suk Bang),김근성(Keun-Sung Kim),박성수(Sung-Soo Park) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.5
남해산 생마늘 및 흑마늘의 일반성분 분석, 아미노산 함량 및 마늘의 주요 황 함유 생리활성물질인 수용성 S-allyl-L-cysteine(SAC) 및 지용성 diallyl-disulfide(DADS)의 함량을 분석하여 발효 전후 이들 성분에 대한 함량 변화를 비교하였다. 일반성분의 함량은 생마늘과 자가숙성발효마늘에서 큰 변화가 없었다. 흑마늘 내의 17종 아미노산중 13종 아미노산 함량이 증가하였다. 특히 함황아미노산인 cysteine 및 methionine의 함량(㎎/100 g)이 각각 295.25±5.08에서 381.86±14.86로, 47.2±3.92에서 66.6±1.08로 증가하였고, histidine의 함량(㎎/100 g)은 319.19±5.42에서 796.62±7.01로 크게 증가하였다. SAC 및 DADS의 함량(㎍/g)은 245.35±1.35에서 522.51±1.19로, 0.275±0.015에서 8.710±0.45로 각각 2배와 30배 이상으로 생마늘에 비하여 흑마늘내의 함량이 매우 큰 폭으로 증가하였다. 이러한 현상은 자가숙성발효라는 제조과정에서 생마늘의 alliin이 1차적으로 효소와 기타 화학적 반응에 의해 allicin으로 전환된 후, allicin으로부터 다른 일련의 복합적인 화학반응에 의하여 많은양의 SAC 및 DADS가 생성된 결과에 의하여 나타났다고 할 수 있다. 또한 자가숙성 발효 과정 중 이러한 SAC 및 DADS 등과 같은 마늘 내 함황화합물들의 지표물질 생성확인은 인체에 유익한 생리활성을 보유한 많은 종류의 다른 함황화합물들이 동시에 흑마늘 내에서 생성되었음을 암시한다. 그러나 흑마늘의 제조과정은 85~95%의 습도, 60~70 ℃에서 40여일 정도 장기간의 숙성이 필요하다. 이러한 숙성과정은 특정 미생물 또는 효소를 이용하여 단축할 필요가 있다. 또한 SAC 및 DADS 등과 같이 생리활성이 높은 함황화합물들의 함량을 증가시킬 수 있는 새로운 가공법에 대한 연구가 필요하다. Garlic (Allium sativum L.) is one of the oldest cultivated plants and has been used throughout the world as a food supplement and a folk medicine for thousands of years. Raw garlic has been processed into a variety of commercial garlic products for consumer convenience. The latest new processing technology, "spontaneous short-term fermentation", has been developed to process raw garlic into black garlic. The physiologically active effects of garlic have been attributed to its organosulfur compounds. In this study, the proximate compositions and the total amino acid content of raw Namhae garlic and black garlic were determined. The two major organosulfur compounds of garlic, S-allyl-L-cysteine (SAC), and diallyl-disulfide (DADS), were also analyzed using RP-HPLC. The proximate compositions were not different between raw and black garlic. The amount of 13 amino acids was greater in black garlic than in raw garlic among a total of 17 amino acids considered. The black garlic had 2-fold higher levels of SAC and 30-fold higher levels of DADS than the raw garlic. Therefore, it is suggested that consuming black garlic produced by spontaneous short-term fermentation is more effective than consuming raw garlic, in order for consumers to take more physiologically active organosulfur compounds (SAC and DADS), which are the compounds that are good for consumer health.
자궁경부 원추형 생검수술후 재발 방지를 위한 인유두종바이러스 검사 시기와 대책에 대한 고찰
김소영 ( So Young Kim ),송은섭 ( Eun Seop Song ),최석진 ( Suk Jin Choi ),김근성 ( Keun Sung Kim ),이기은 ( Ki Eun Lee ),김석모 ( Seok Mo Kim ),박지현 ( Jee Hyung Park ),송상용 ( Sang Yong Song ),송윤섭 ( Yun Seob Song ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.7
목적: 자궁경부 원추형 생검수술후 언제 인유두종바이러스 (HPV) 검사를 하는 것이 좋은가를 알고자 하였다. 연구방법: 1997년부터 2004년까지 A병원 산부인과에서 원추형생검을 Large Loop Excisions of the Transformation Zone (LLETZ)에 의해 시행한 환자를 대상으로, 시술 전 Pap과 HPV 아형을 HPV oligonucleotide microarray (Biomedlab Co., Seoul, South Korea)를 이용하여 검사하였고, 시술 후 3개월마다 HPV 아형검사를 검사하였다. 결과: 8년간 120명의 환자가 이 연구에 참여하였다. 8명은 병변이 없었고, 9명은 자궁경부 상피내종양 (CIN) 1, 17명은 CIN2, 74명은 CIN3, 10명은 미세침윤암, 그리고 2명은 선암이 자궁경부상피내에 국한되어 있었다. LLETZ를 하기 전에 HPV는 85.0%에서 양성이고, 가장 흔한 아형은 16번으로 전체의 40.8%를 차지하였다. LLETZ후에는 1,307 개월에 걸쳐서 190회의 HPV 검사가 실행되었으며 (평균 1회 검사당 6.9개월), 95회 (79.2%)에서는 음성을 25회 (20.8%)에서는 양성을 보였다. 그리고 음성 환자들의 약 80%에서 LLETZ후 6개월 내에 음성을 보였다. 결론: LLETZ로 HPV 음성 되는 환자의 80%가 6개월 내 음성이 되므로, HPV 아형 검사는 시술 후 6개월째부터 하는 것이 좋을 것으로 사료된다. Objective: To know when human papillomavirus (HPV) testing should be done after conization. Methods: Between 1997 to 2004, Large Loop Excisions of the Transformation Zone (LLETZ) were done for conization to women with cervical pathology at A University Hospital. The Pap and HPV typing were done before LLETZ procedures. After conizations, HPV typing were planned to be done every 3 months. Every HPV typing was done by HPV oligonucleotide microarray (Biomedlab Co., Seoul Korea). Results: For 8 years, 120 LLETZ were enrolled in this study. There were 8 cases of no neoplasm, 9 cases of CIN 1, 17 cases of CIN 2, 74 cases of CIN 3, 10 cases of microinvasive cervix cancer, and 2 cases of adenocarcinoma in situ. HPV DNA before LLETZ procedures was found about 85.0% and subtype 16 was the most common type among the patients with cervical lesion (40.8%). After LLETZ, 190 HPV typing were done through 1,307 total months (average, 6.9 months/typing). 95 (79.2%) cases had negative results, and 25 (20.8%) cases had positive results. Our data showed that, after conization, about 80% turned out to negative in 6 months. Conclusion: Our data suggested HPV DNA testing should be done after 6 months of LLETZ, as about 80% were destined to negative in 6 months.