오미자를 이용한 Hg(I), Pb(II), U(VI) 이온들의 흡착

김관천,김준태,Kim, Kwan-Chun,Kim, Joon-Tae 대한환경위생공학회 2007 대한환경위생공학회지 Vol.22 No.1

This study was examined adsorption ability of heavy metal Hg(I), Pb(II), and U(Vl) ion use of fruit from schizandra chinensis, The fruits of schizandra chinensis sample used breaking into fragments $50{\sim}100$ mesh. The sample solution was mixed fruits of schizandra chinensis and heavy metal ion. Each heavy metal ion of into solution was quantum analysis with ICP-AES. As the result, each condition of maxium adsorption ability of heavy metal ion was high in the range of pH 5-7, adsorption time was about 15 minutes, and the optimum temperature was $100^{\circ}C$. The heavy metal ion was increased adsorption in order of increasing concentration and in ethanol solution better than in aqueous solution.

NADPH의 산화반응과 아질산 생성반응에 의한 Metallothionein의 항산화적 기능 확인

김관천(Kwan-Chun Kim),김준태(Joon-Tae Kim),김희정(Hee-Joung Kim) 대한환경위생공학회 2006 대한환경위생공학회지 Vol.21 No.1

Metallothioneins(MTs) belong to the class of low molecular weight proteins. Recently, it has been suggested that MTs may play a direct role in cellular defense against oxidative stress by functioning as antioxidants. Oxidative damage to different cellular components makes a major contribution to many pathogenenesses. Several studies have demonstrated that MT is able to quench a wide range of reactive oxygen species at a higher efficiency than other well known antioxidants such as superoxide dismutate(SOD). The present study was designed to evaluate the effect of MT on the activities of the reactive oxygen species removal system. MT showed the scavenging of superoxide in the SOD assay system in the presence or absence of SOD. When MT was added to nicotinamide adenine dinucleotide phosphate(NADPH) oxidation system in presence of fixed amount of SOD increase the breakdown rate of superoxide. When MT was added to the system that form nitrite from hydroxylammonium chloride, the formation of nitrite was inhibit. We concluded that the function of MT as antioxidant might have an effect on the level of superoxide scavenging.

정향(Clove) 추출물이 티로시나아제 유전자의 발현에 미치는 효과

진종언,이정용,김관천,Chin, Jong-Eon,Lee, Jeong-Yong,Kim, Kwan-Chun 대한환경위생공학회 2007 대한환경위생공학회지 Vol.22 No.1

Clove extract by methanol increased expression of the tyrosinase gene on B16 mouse melanoma cells containing tyrosinase promoter. $10{\mu}g/mL$ and $100{\mu}g/mL$ of the extract showed expression rate of the tyrosinase gene about 138% and 245%, respectively, compared with control. At $500{\mu}g/mL$, expression rate of the extract was impossible to measurement by high cytotoxicity. The solvent fraction of methylene chloride also exhibited highly expression rate as methanol extract. However, the solvent fractions of butyl alcohol and water showed repressive effect on expression of tyrosinase gene at $500{\mu}g/mL$. In MTT assay, cell survival rate of the extract exhibited similar to expression rate of tyrosinase gene. That is, $10{\mu}g/mL$ and $100{\mu}g/mL$ of the extract showed the cell survival rate about 128% and 187%, respectively.

섬진강 하구역에서 환경요인 및 기초생산성의 변화

양성렬,송환석,김관천,박철,문창호,YANG SUNG RYULL,SONG HWAN SEOK,KIM KWAN-CHUN,PARK CHUL,MOON CHANGHO 한국해양학회 2005 바다 Vol.10 No.3

섬진강 하구역에서 일차생산을 조절하는 환경요인과 기작을 포악하기 위하여 엽록소 a, 영양염, pH, 부유물질, 용존산소, 염분과 일차생산을 2001년 2월, 4월, 8월, 10월 네 차례에 걸쳐서 측정하였다. 조사기간 중 일차생산의 범위는 50.7에서 14,203.3 mg C $m^{-3} day^{-1}$의 범위를 보여주었다. 다른 하구역과는 대조적으로 본 조사지역에서는 대부분의 시기에 높은 수층 투명도로 인하여 광 조건이 광합성의 제한요인으로 작용하지 않았다. 염분이 10에서 20 psu사이의 지역에서 가을철 대증식이 발생하였는데, 이 현상은 매년 나타나는 것으로 보이며 그 기작에 대하여 추가적인 연구가 필요한 것으로 생각된다 일차생산을 조절하는 주된 요인 중의 하나인 영양염의 거동은 염분에 의하여 지배되는 것으로 보인다. 질소계 영양염의 주된 공급원은 섬진강으로부터의 담수에 의한 것으로 보이는 반면, 인은 광양만 유역의 산업폐수로부터 기인한 것으로 판단된다. 본 조사지역에서 식물플랑크톤에 의한 일차생산은 시간적 공간적인 변화를 보이며, 수층의 투명도와 밀접한 관계를 나타내고, 인접한 광양만 내의 연안역에 비교하여 상대적으로 높은 값을 나타내었다. To investigate the aquatic environmental factors and processes controlling primary production in the Seomjin River estuary, chlorophyll a, nutrients, pH, SS, DO, temperature, salinity and primary productivity were measured in February, April, August and October, 2001. Primary productivity values ranged between 50.7 and 14,120.3 mg C $m^{-3} day^{-1}$ during the sampling period. In contrast to other estuaries, light condition did not seem to be the important limiting factor far primary production due to high water-column transparency during most of the time. The autumn bloom occurred in regions where salinity values ranged between 10 and 20 psu. This phenomenon appeared to develop every year and deserves further investigation. The behavior of nutrients, which is one of the major factors controlling the primary productivity, appeared to be governed by salinity regimes. The main source of nitrogenous nutrients seemed to be the freshwater runoff from the Seomjin River. However, that of phosphorus seemed to be from the industrial wastewater in Gwangyang area. The primary pro-duction of phytoplankton in the study area varied with space and time, showing a close correlation with water column transparency, and exhibited higher values compared to those of adjacent coastal regions in Gwangyang Bay.

대두(Glycine maxim) 추출물이 티로시나아제 프로모터 활성에 미치는 효과

진종언 ( Jong Eon Chin ),김관천 ( Kwan Chun Kim ) 한국식품영양학회 2008 韓國食品營養學會誌 Vol.21 No.3

The methanolic extract of Glycine maxim increased the expression of the promoter in B16 mouse melanoma cells harboring a tyrosinase promoter. Extract concentrations of 10 μg/㎖ and 100 μg/㎖ resulted in tyrosinase promoter expression rates of approximately 113% and 184%, respectively, as compared to the control. The fraction layers consisting of butyl alcohol and methylene chloride improved expression effects on the tyrosinase promoter. In particular, the butyl alcohol fraction evidenced a high expression rate at 100 μg/㎖. In the MTT assay, the methanolic extract did not evidence cytotoxicity at concentrations under 500 μg/㎖. Therefore, the results observed with the extract of Glycine maxim showed that the substance exerted a positive effect on the tyrosinase promoter.

1-Aza-15-Crown-5-Styrene(위험물)-DVB 수지에 의한 희토류 금속 이온들의 흡착 특성

노기환 ( Gi Hwan Roh ),김관천 ( Kwan Chun Kim ),김선화 ( Sun Hwa Kim ),김준태 ( Joon Tae Kim ) 한국공업화학회 2009 공업화학 Vol.20 No.1

율피 추출물이 티로시나아제 유전자 발현에 미치는 효과

진종언(Jong-Eon Chin),김관천(Kwan-Chun Kim) 대한환경위생공학회 2005 대한환경위생공학회지 Vol.20 No.3

Chestnut bark extract by methanol repressed the expression of tyrosinase gene of B16 mouse melanoma cell containing tyrosinase promoter. 10 ㎍/㎖, 100 ㎍/㎖, 1 ㎎/㎖ of the extract repressed expression of tyrosinase gene about 38%, 47%, and 78%, respectively, compared with control. In the MTT assay, the same extract exhibited very low cytotoxicity at 1 ㎍/㎖, 10 ㎍/㎖, 100 ㎍/㎖, and 1 ㎎/㎖, respectively. The fractions of Methylene chloride and ethyl acetate did not showed the repressive effect on the expression of tyrosinase gene, but the fraction of butyl alcohol repressed highly at 10 ㎍/㎖ and 100 ㎍/㎖.

Cryptand 이온교환 수지에 의한 U(Ⅵ), Pb(Ⅱ), Ce(Ⅲ) 금속 이온들의 흡착

김준태(Joon-Tae Kim),김해진(Hae-Jin Kim),김관천(Kwan-Chun Kim) 한국환경관리학회 2008 環境管理學會誌 Vol.14 No.2

1%, 2%, 10% 및 20%의 가교도를 가진 스타이렌(제4류 위험물) 디비닐벤젠 공중합체에 1-aza-18-crown-6 거대 고리 리간드를 치환반응으로 결합시켜 수지들을 합성하였으며, 이들 수지의 합성은 염소 함량과 원소 분석 그리고 IR-스펙트럼으로 확인하였다. 수지 흡착제에 대한 금속 이온의 흡착에 미치는 pH, 시간 그리고 수지의 가교도에 따른 영향들을 조사한 결과 금속 이온들은 pH 3 이상에서 큰 흡착율을 보였으며, 금속 이온들의 흡착 평형은 2시간 정도였다. 한편, 에탄올 용액에서 수지에 대한 흡착 선택성은 우라늄(UO₂²?) > 납(Pb²?) > 세륨(Ce³?) 이온이었고, 금속 이온의 흡착력은 1%, 2%, 10% 및 20%의 가교도 순이었다. Resins were synthesized with 1-aza-18-crown-6 macrocyclic ligand attached to styrene(dangerous matter) divinylbenzene(DVB) copolymer with crosslink of 1%, 2%, 10%, and 20% by substitution reaction. The synthesis of these resins was confirmed by content of chlorine, element analysis, and IR-spectrum. The effects of pH, time and crosslink on adsorption of metal ion by the synthetic resin adsorbent were investigated. The metal ion was showed fast adsorption on the resins above pH 3. The optimum equilibrium time for adsorption of metallic ions was about two hours. The adsorption selectivity determined in ethanol solution was in increasing order of uranium(UO22+) > lead(Pb2+) > cerium(Ce3+) ion. The adsorption was in the order of 1%, 2%, 10%, and 20% crosslink resin.

물 소화약제로부터 Cryptand 이온교환수지의 Mg(Ⅱ), Al(Ⅲ) 및 Pb(Ⅱ) 흡착특성

김준태(Joon-Tae Kim),김관천(Kwan-Chun Kim) 대한환경위생공학회 2008 대한환경위생공학회지 Vol.23 No.1

Resins were synthesized with 1-aza-15-crown-5 macro cyclic ligand attached to styrene(dangerous matter) divinylbenzene(DVB) copolymer with crosslink of 1, 2, 6 and 15% by substitution reaction. The synthesis of these resins was confirmed by content of chlorine, element analysis, and IR-spectrum. The effects of pH, time and crosslink on adsorption of metal ion from water fire extinguishing agent by the synthetic resin adsorbent were investigated. The metal ion was showed fast adsorption on the resins above pH 3. The optimum equilibrium time for adsorption of metallic ions was about two hours. The adsorption selectivity determined in water was in increasing order of Mg²? > Al³? > Pb²?. The adsorption was in the order of 1, 2, 6, and 15% crosslink resin.

beta - Galactosidase의 고정화 및 응용에 관한 연구 - 제1보 : Aspergillus niger CAD 1의 효소생산 조건 및 효소학적 성질

이용규(Yong-Kyu Lee),전순배(Soon-Bae Chun),최원기(Won-Ki Choi),정기철(Ki-Chul Chung),배석(Suk Bae),김관천(Kwan-Chun Kim) 한국식품영양과학회 1986 한국식품영양과학회지 Vol.15 No.4

토양에서 분리한 여러가지 곰팡이류 가운데 beta-galactosidase 생성력이 가장 좋은 Aspergillus niger CAD 1을 효소 생산 균주로 선정하였다. 이 균주의 효소 생산 최적 배양조건은 밀기울에 0.5% 탈지 분유를 첨가한 배지를 30℃에서 72시간 정치배양하는 것이었다. 아세톤으로 침전시킨 조효소(crude enzyme)는 일차로 DEAE-cellulose, 2차로 Sephadex G-100 gel filtration에 의하여 1,387배로 정제되었고 이때의 수율은 6.2%이었다. 정제된 효소의 최적 온도는 45℃, 최적 pH는 4.5이었으며, 기질로서 ONPG와 유당에 대한 Km값은 각각 3.57×10^(-3)M과 83.3×10^(-3)M, Vmax값은 각각 33.0 unit/㎎ protein과 15.38 unit/㎎ protein이었다. 활성화 에너지는 9,900㎈/㏖이었으며 효소활성 및 안정제로 금속이온을 필요로 하지 않았다. 50㎖의 탈지유, 4.8% 유당 용액, 유청용액에 효소 5㎖(182 unit/㎖)를 침가하여 45℃에서 10시간 반응시켰을 때의 유당 분해율은 각각 65%, 70%, 78%를 타타내었다. A strain of Aspergillus niger CAD 1 which produces considarable amount of beta-galactosidase was selected from extracellular beta-galetaosidase producing fungi isolated from soil. Optimal conditions for the enzyme from Aspergillus niger CAD 1 were the growth in wheat bran supplemented with 0.5% skim milk powder at 30℃ for 72 hrs. The crude enzyme was purified 1.387 fold through DEAE-cellulose and Sephadex G-100 chromatography and its recovery was 6.2%. The optimal pH and temperature for the purified enzyme were pH 4.5 and 45℃, respectively. The Km and Vmax on ONPG were 3.57 × 10^(-3)M and 33.0 unit/㎎ protein, whereas those on lacose were 83.3 × 10^(-3)M and 15.33 unit/㎎ protein, respectively. The activation energy for the enzyme was 9,900 ㎈/㏖ and the enzyme had no metal ion requirement for its activity and stability. The hydrolysis of lactose in skim milk, 4.8% lactose solution and acidic whey were 65%, 70% and 78% after 10 hrs incubation at 45℃, when 182 units of the enzyme were used 50㎖ of the substrate solutions.