홍성 북동부 농촌 지역 지하수의 질산성 질소 오염과 수리지구화학적 특성

기민규,고동찬,윤희성,김현수,Ki, Min-Gyu,Koh, Dong-Chan,Yoon, Heesung,Kim, Hyun-Su 한국지하수토양환경학회 2013 지하수토양환경 Vol.18 No.3

Spatial and temporal characteristics of nitrate contamination and hydrogeochemical parameters were investigated for springs and surficial and bedrock groundwaters in northeastern part of Hongseong. Two field investigations were conducted at dry and wet seasons in 2011 for 120 sites including measurement of field parameters with chemical analyses of major dissolved constituents. Nitrate concentrations were at background levels in springs while 45% of bedrock groundwater and 49% of surficial groundwater exceeded the drinking water standard of nitrate (10 mg/L as $NO_3$-N). The difference in nitrate concentrations between surficial and bedrock groundwater was statistically insignificant. Cumulative frequency distribution of nitrate concentrations revealed two inflection points of 2 and 16 mg/L as $NO_3$-N. Correlation analysis of hydrogeochemical parameters showed that nitrate had higher correlations with Sr, Mg, Cl, Na, and Ca, in surficial groundwater in both dry and wet season. In contrast, nitrate had much weaker correlations with other hydrogeochemical parameters in bedrock groundwater compared to surficial groundwater and had significant correlations only in wet season. Temporally, nitrate and chloride concentrations decreased and dissolved oxygen (DO) increased from dry season to wet season, which indicates that increased recharge during the wet season affected groundwater quality. Aerobic conditions were predominant for both surficial and bedrock groundwater indicating low natural attenuation potential of nitrate in the aquifers of the study area.

지하수 오염 취약성 기법의 비교 적용 연구: 충남 홍성군 금마면 일대에의 적용

기민규,윤희성,고동찬,함세영,이충모,김현수,Ki, Min-Gyu,Yoon, Heesung,Koh, Dong-Chan,Hamm, Se-Yeong,Lee, Chung-Mo,Kim, Hyun-Su 한국지하수토양환경학회 2013 지하수토양환경 Vol.18 No.3

In the present study, several groundwater vulnerability assessment methods were applied to an agricultural area of Gumma in Korea. For the groundwater intrinsic vulnerability assessment, the performance of DRASTIC, SINTACS and GOD models was compared and an ensemble approach was suggested. M-DRASTIC and multi-linear regression (MLR) models were applied for the groundwater specific vulnerability assessment to nitrate of the study site. The correlation coefficient between the nitrate concentration and M-DRASTIC index was as low as 0.24. The result of the MLR model showed that the correlation coefficient is 0.62 and the areal extents of livestock farming and upland field are most influential factors for the nitrate contamination of groundwater in the study site.

고창·부안 갯벌에 자생하는 염생식물 칠면초 근권 및 근면으로부터 내염성 세균 분리와 그 특성화

기민규 ( Min-gyu Ki ),이혜리 ( Hyeri Lee ),조아현 ( Ahyeon Cho ),운노타쯔야 ( Tatsuya Unno ),이지훈 ( Ji-hoon Lee ) 한국응용생명화학회 2017 Journal of Applied Biological Chemistry (J. Appl. Vol.60 No.2

고창·부안 갯벌에 자생하는 칠면초의 근권 및 근면으로부터 내염성을 지니는 9 균주를 분리하였다. 16S rRNA 유전자를 이용한 계통분석으로 분리된 균주는 Vibrio 속과 Bacillus 속으로 분류되었다. 분리된 균주 중 Vibrio와 Bacillus로 나누어 각각 대표성을 나타낼 수 있는 균주를 선택하여 염 농도에 따른 최적 생장조건을 평가한 결과, 균주 JRS-1 (Vibrio neocaledonicus)은 총 염분 4-6%에서 높은 생장률을 보였다. 균주 JRL-2(Bacillus thuringiensis)는 염분 농도 증가에 따라 생장이 증가하였으며, 7%에서 최대 생장률을 보이고 8%에서는 감소하는 경향을 보였다. 분리된 균주들은 기존에 서식하던 토양의 염 농도인 3% (w/v) 보다 더 높은 염분에도 견딜 수 있는 내염성을 보였다. 또한 분리균주에 대한 생화학적 기질 이용 형태에 분석을 통해 triglyceride, ρ-nitrophenyl-α, D-glucoside, ρ-nitrophenyl-β,D-glucoside 이용 등 식물과의 특이적인 상호작용을 나타내는것으로 간주할 수 있는 반응들을 확인하였다. Nine strains of high concentrations of salt-tolerant bacteria were isolated from the rhizosphere and rhizoplane of the halophyte plant Suaeda japonica grown in Gochang · Buan tidal flat. The isolated bacteria were classified as genera Vibrio (strains JRS-1, -2, -3, -4, and -5, and JRL-1 and -4) and Bacillus (strains JRL-2 and -3) based on the 16S rRNA gene sequence similarity. The optical growth condition for salt concentration was examined on the selected, representative strains. Strain JRS-1 with the closest relative of Vibrio neocaledonicus showed the highest growth rate at the total salt concentration of 6% among the incubation conditions of 3-8% salt concentrations. Strain JRL-2 with the closest relative of Bacillus thuringiensis showed the tendency that growth rate increased with increasing salt concentrations and the maximum growth rate at 7% of the total salt concentration. The isolated bacteria showed salt-resistances to higher salt concentrations than their habitat soils with 3%. In addition, we identified evidences of potentially plant interaction-relevant enzymatic activities, from utilization of some substrates rich in plants, such as triglyceride, ρ-nitrophenyl-α,D-glucoside, and ρ- nitrophenyl-β,D-glucoside.

지하수 오염취약성평가 기법의 비교 적용 연구

기민규(Min-Gyu Ki),윤희성(Heesung Yoon),김용철(Yongcheol Kim) 대한지질학회 2014 대한지질학회 학술대회 Vol.2014 No.10

농촌 지역 지하수내 질산성 질소와 관련 수질 인자의 월간 변동 특성

기민규(Min-Gyu Ki),고동찬(Dong-Chan Koh),윤희성(Heesung Yoon),하규철(Kyoochul Ha),김현수(Hyun-Su Kim) 대한지질학회 2013 대한지질학회 학술대회 Vol.2013 No.10

LM 미생물의 환경영향 모사: 대사체와 토양미생물군 분석

이지훈,기민규 한국환경농학회 2019 한국환경농학회지 Vol.38 No.3

BACKGROUND: Living modified microorganisms (LMMs) have been focused in two very different aspects of positive and negative effects on ecology and human health. As a model experiment, wild type and a foreign origin geneharboring modified E. coli strains were subjected to comparison of their metabolomes and potential effects on soil microbiota in the laboratory sets. This study assumes the unintentional release of LMMs and tries to suggest potential effects on the soil microbiota even at minimal settings. METHODS AND RESULTS: Metabolomes from the wild type and LM E. coli were analyzed by NMR and the profiles were compared. In the laboratory soil experiments, the two types of E. coli were added to the soils and monitored for the bacterial community compositions. Those metabolomic profiles did not show significant differences. The microbial community structures from the time series soil DNAs for both the sets using wild type and LMO also did not indicate significant changes, but minor by the addition of foreign organisms regardless of wild or LMO. CONCLUSION: Even if the recombinant microorganism (LMO) is released into the soil environment, the survival of microorganisms in the environment would be one of the major factors for the transfers of foreign genes to other organisms and diffusion into the soil environment.

Construction of Aligned Database of dsrA, a Gene Encoding Dissimilatory Sulfite Reductase Alpha Subunit, for Metagenomic Studies of Sulfate-Reducing Bacteria

이지훈,김보아,기민규 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.4

A sulfite reductase gene sequence database, whichcould facilitate analysis of metagenomic data of a functional genefrom sulfate-reducing bacteria, is described. The database contains127 aligned nucleotide sequences of a gene (dsrA), encoding adissimilatory sulfite reductase alpha subunit, retrieved fromReference Sequence of GenBank that provides only well-annotatedgenome sequences. The dsrA gene sequences were screened forlength and aligned using four multiple sequencing alignmentprograms, Mafft, Muscle, Mothur, and Clustal Omega. In additionto the dsrA gene sequences, 16S ribosomal RNA (rRNA) genesequences for the bacterial species appearing in the database werealso retrieved from the GenBank and aligned by a public databaseof SILVA in Mothur to examine any potential relationshipbetween taxonomy of the bacteria and distribution of a functionalgene. The aligned dsrA and 16S rRNA gene sequences were usedto construct neighbor-joining phylogenetic trees for comparison ofthe alignments for relative agreement among the aligned sequencedatabases. This method of aligned reference database constructionusing public database can help provide better insight intomicrobial communities by suggesting an alternative approach ofstudying metagenomic analysis of functional genes involved inimportant ecological processes.

청주 상대리 수막재배지의 지중 침투형 갤러리 설치와 예비 주입시험

문상호,김용철,김성윤,기민규,Moon, Sang-Ho,Kim, Yongcheol,Kim, Sung-Yun,Ki, Min-Gyu 대한자원환경지질학회 2016 자원환경지질 Vol.49 No.6

우리나라 수막재배 지역은 성수기에 지속적인 지하수 사용으로 인해 수막재배 말기에 지하수위 하강과 더불어 지하수 고갈 문제로 항시 어려움을 겪고 있다. 최근에 국내에서는 이러한 지역에서의 지하수 부족 현상을 해결하기 위해 몇 가지 인공함양 방법들이 적용되고 있다. 이 연구에서는 인공함양 방식 중 하나인 지중 침투형 갤러리 설치를 소개하고, 이 중 3개 갤러리에서의 인공 주입시험 결과를 이용하여 인공함양의 효율성을 예비적으로 평가하였다. 연구지역은 청주시 상대리 수막재배지이며, 갤러리의 규모는 가로${\times}$세로${\times}$깊이 $50cm{\times}300cm{\times}50cm$이다. 갤러리는 비닐하우스 동 사이의 이격 공간을 활용하여 설치하였으며, 터파기 작업, 자갈석과 규사 메움, 피조미터와 상부 부직포 설치 순서로 진행하였다. 3개 갤러리에 대한 인공 주입시험은 1차 예비, 2차 단계, 3차 장기 주입시험으로 구성된다. 1차 예비 시험에서 B 갤러리는 주입율 $33.29{\sim}33.84m^3/d$, $45.60{\sim}46.99m^3/d$일 때 수위 상승이 각각 20 cm, 30 cm, C 갤러리는 주입율 $21.1m^3/d$, $33.98m^3/d$, $41.69m^3/d$일 때 수위 상승이 각각 0 cm, 16 cm, 33 cm, D 갤러리는 주입율 $48.10m^3/d$, $52.23m^3/d$일 때 수위 상승이 각각 29 cm, 42 cm 정도를 유지한 것으로 나타났다. 단계 및 장기 주입시험 결과를 이용하여 각 갤러리에 대한 주입율과 수위 관계식이 정량적으로 도출되었으며, 이와 같은 인공 주입시험 결과는 수막재배 기간 중에 사용 후 배출되는 지하수가 갤러리를 통해 자연적으로 주입되는 수량을 추정하는 데에 정량적으로 활용될 수 있을 것으로 기대된다. Most of water curtain cultivation (WCC) area in Korea has been inveterately suffering from the gradual draw-down of groundwater level and related shortage of water resources at the late stage of WCC peak time. To solve this problem, artificial recharge techniques has been recently applied to some WCC area. This study introduces infiltration gallery, which is one of the artificial recharge methods, and tentatively examined the effectiveness of three galleries installed at Sangdae-ri WCC area of Cheongju City. Seven galleries are set up at each empty space between eight vinyl houses in this area and its dimension is designed as 50 cm in each width and height and 300 cm in each length. Installation process was including bed excavation, backfill with gravels and silica sands, and completion of gallery by equipment of piezometer and covering with non-woven cloth. For each B, C, D gallery, 3 types of test including preliminary, four step and one long-term injection were performed. The first preliminary test showed the rough relations between injection rates and water level rise as follows; 20 cm and 30 cm level rise for $33.29{\sim}33.84m^3/d$ and $45.60{\sim}46.99m^3/d$ in B gallery; 0 cm, 16 cm and 33 cm level rise for $21.1m^3/d$, $33.98m^3/d$ and $41.69m^3/d$ in C gallery; 29 cm and 42 cm level rise for $48.10m^3/d$ and $52.23m^3/d$ in D gallery. Afterwards, more quantitative results estimating effectiveness of artificial recharge were reasoned out through stepped and long-term injection tests, which is expected to be employed for estimating water quantity re-injected into the aquifer through these galleries by natural injection over the period of WCC peak time.

해외사례를 통해 본 국내 인공함양수 수질기준의 합리적 방향에 대한 제언

김용철(Yongcheol Kim),기민규(Min-Kyu Ki),김성윤(Sung-Yun Kim) 대한지질학회 2015 대한지질학회 학술대회 Vol.2015 No.10

청주시 상대리 수막재배지의 지질특성과 지중침투형 갤러리 주입시험 사례

문상호(Sang-Ho Moon),김용철(Yongcheol Kim),김성윤(Sung-Yun Kim),기민규(Min-Gyu Ki) 대한지질학회 2016 대한지질학회 학술대회 Vol.2016 No.10