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난소의 자궁내막종에 대한 다양한 치료적 적용에 따른 체외수정 및 배아이식술 결과의 비교 연구
이방현,권혁찬,이재현,김보현,이상희,박민혜,이병관,임정애,Lee, Bang-Hyun,Kwon, Hyuck-Chan,Lee, Jae-Hyun,Kim, Bo-Hyun,Lee, Sang-Hee,Park, Min-Hye,Lee, Byung-Kwan,Lim, Jung-Ae 대한생식의학회 2004 Clinical and Experimental Reproductive Medicine Vol.31 No.2
Objective: To compare COH characteristics and IVF outcomes among IVF-ET patients who were treated with various therapeutic modalities for ovarian endometriomas and to propose effective pre-cyclic therapeutic modalities to improve IVF-ET outcomes in the patients with ovarian endometriomas. Methods: All cases that had undergone IVF-ET after laparoscopy between January 1997 to August 2003 were reviewed. Forty-eight patients with tubal factor were assigned to Group I. Twenty seven, 22 and 38 patients diagnosed as severe pelvic adhesion with ovarian endometriomas by laparoscopy received only medical therapy (Group II), cyst aspiration (Group III), and sclerotherapy (Group IV), respectively. Laparoscopic cystectomy was performed in 20 patients (Group V). Resistance index was measured on day administering hCG. Results: As compared with Group I, in Group II resistance index increased (p<0.05) but number of oocytes, good-quality oocyte ratio (mature and intermediate oocytes/total retrieval oocytes), fertilization rate, and embryo development rate decreased (p<0.05). In Group III fertilization rate and embryo development rate decreased (p<0.05). There was no difference between Group IV and Group I in all parameters except basal FSH which increased (p<0.05). In Group V basal FSH, and resistance increased (p<0.05) and number of oocytes and good-quality oocytes ratio decreased (p<0.05). Conclusion: Sclerotherapy is an effective therapeutic option which can be done prior to IVF-ET cycles in the patients with ovarian endometriomas. Further studies on a large scale are necessary to confirm these data.
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한국여성에서 자궁경부암의 고위험군 인유두종 바이러스의 동정
이상희 ( Sang Hee Lee ),이방현 ( Bang Hyun Lee ),윤효진 ( Hyo Jin Yoon ),박민혜 ( Min Hye Park ),권혁찬 ( Hyuck Chan Kwon ),임정애 ( Jung Ae Lim ),이병관 ( Byung Kwan Lee ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.12
목적 : 한국 여성에서 자궁경부암과 관련되어 발현되는 하부생식기 HPV 형과 자궁경부암의 고위험군 HPV 형을 분류하고자 한다. 연구 방법 : 본원에서 자궁경부암 검사를 시행하는 환자들을 대상으로 Pap test와 PCR 방법에 의한 HPV- DNA 검사를 시행하였고 HPV-DNA 검사에서 양성으로 판명된 경우 sequencing 방법에 의한 HPV-genotyping과 자궁경부 조직검사를 시행하였다. 결과 : HPV 감염의 주된 연령군은 40대였고 ( Objective : To identify genital HPV types and high risk group of HPV associated with cervical cancer in Korean women. Methods : Both Pap test and HPV-DNA test using PCR assay were performed as screening test for cervical cancer in this clinic. When patien
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생쥐 배아의 체외배양시 저농도 산소 환경이 배아 발달에 미치는 영향
유정현(Jong Hyun Woo),황경주(Kyung Joo Hwang),양현원(Hyun Won Yang),이치형(Chi Hyeong Lee),양정인(Jeong In Yang),권혁찬(Hyuck Chan Kwan),오기석(Kie Suk Oh) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.12
N/A Objective: It is known that mouse embryos before implantation develop in a low oxygen environment of 3- 8% concentration and with antioxidant materials such as vitamins, antioxidant enzymes, ferrous binding proteins, and albumin in follicular and tubal fluids. However, the 20% oxygen culture condition with chemically defined media might be produce an abundance of ROS, and leads to developmental delay or developmental block in vitro. In this study, we attempt to elucidate the relationship between intracellular H2O2 production and embryo development in different oxygen culture conditions of mouse embryos. Methods: Prenuclear embryos from C57BL/CBA Fl hybrid and ICR mouse were cultured in incubators which provided 5% carbon dioxide, 20% oxygen and 5% carbon dioxide, 5% oxygen. Measurement of H2O2 level in a embryo was performed with DCHFDA(2,7 -dichlorodihydroflourescein diacetate)and analyzed with Quanti-cell 700, and the number of blastomeres was counted with DAPI( 4, 6'-diamidino-2-phenylindole). Results: Oxygen concentration of the culture medias was significantly higher in the 20% oxygen environment compared to that of 5% oxygen environment. Culture of mice embryos in high oxygen condition leads to high HO concentrations at 2 cell stage and developmental delay or 2-cell block regardless of the strain. But in a 5% oxygen environment, which is similar to in-vivo conditions HO production was suppressed continuously through out culture and development of embryos was definitely improved. Conclusion: These results suggest that there is a difference in the production of ROS or protective mechanism according to the mouse strains and stage of development, and it is thought that in-vitro culture in 5% oxygen environment provides stable in vivo equilibrium but in a 20% oxygen environment there is production of ROS which overcome the protective mechanism which leads to cellular damage and embryo developmental delay.
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