혈소판유래성장인자-BB가 골간질세포와 치주인대세포의 성상에 미치는 영향

권영혁,박준봉,Kwon, Young-Hyuk,Park, Joon-Bong 대한치주과학회 1996 Journal of Periodontal & Implant Science Vol.26 No.4

The main goal of periodontal therapy is to restore the lost periodontal tissue and establish the attachment appratus. Current acceptable therapeutic techniques are included : removal of diseased soft tissue, demineralization of exposed root surface, using the barrier membrane for preventing the downgrowth of gingival epithelial cell, insertion of graft materials as a scaffolding action, and biological mediators for promoting the cell activity. The latest concept one among them has been studied which based on the knowledge of cellular biology of destructed tissue. Platelet-derived growth factor(PDGF) is one of the polypeptide growth factor which have been reported as a biological mediator to regulate activities of wound healing progress including cell proliferation, migration, and metabolism. The purposes of this study is to evaluate the influences of the PDGF as biological mediator to periodontal ligament and bone marrow cell. Both right and left maxillary first molar were extracted from rat which had treated with 0.4% ${\beta}-Aminopropionitril$ for 5 days, and feeded until designed date to sacrifice under anesthesisa. Periodontal ligament were removed from the extracted socket of the rat, and cultured with Dulbecco's Modified Essential Medium(DMEM) contained with 10% Fetal Bovine Serum, 100U/ml penicillin, $100{\mu}g/ml$ streptomycin, $0.5{\mu}g/ml$ amphotericin-B. Bone marrow cell were culture from bone marrow suspension with which washed out from femur with same medium. The study was performed to evaluate the effect of PDGF to periodontal ligament and bone cell, cell proliferation rate, total protein synthesis, and alkaline phosphatase activity of rat periodontal ligament(PDL) cell and bone stromal(RBS) cell in vitro. The effects of growth factors on both cells were measured at 3, 5th day after cell culture with (control group) or without growth factors(experimental group). The results were as follows: 1. The tendency of cell proliferation under the influence of PDGF showed more rapid proliferation pattern than control at 3 and 5 days after inoculation. 2. The activity of Alkaline phosphatase revealed 14, 80% increased respectively at 3, 5 days culture than control group. Measurements of ALPase levels indicated that PDL cells had significantly higher activity when compared with that of co-culture groups and GF only(P<0.05). And, ALPase activity in 10 days was higher than that of 7 days(P<0.05). 3. The tendency of formation of the mineralized nodule were observed dose-depend pattern of PDL cells. There was statistically significant difference among group l(PDL 100%), 2(PDL 70%:GF 30%), and 3(PDL 50%:GF 50%)(P<0.01). But, there was no difference among group 3, 4(PDL 30%:GF 70%), and 5(GF 100%). 4. Also, the number of nodule was greater in co-culture of PDL 70% and GF 30% than in culture of PDL 70%(P<0.05). From the above results, it is assumed that the PDGF on PDL cells and RMB cell culture. GF stimulates the cell growth, which is not that of PDL cells but GF. And, the activity of ALPase depends on the ratio of PDL cells, and ALPase may relate to the initial phase of nodule formation. Also, it is thought that the calcified nodule formation principally depends on PDL cells, is inhibited by GF, and affected by cell density. In conclusion, platelet-derived growth factor can promote rapid osteogenesis during early stage of periodontal tissue regeneration.

유전성 치은 증식증에 대한 증례보고

권영혁,이만섭,Kwon, Young-Hyuk,Lee, Man-Sup 대한치주과학회 1983 Journal of Periodontal & Implant Science Vol.13 No.1

Guided Bone Regeneration with a PDGF-BB-Ioaded Resorbable Membrane in the Dehisced Implant of the Beagle Dog

권영혁,박준봉,허익,정종평,한수부,이승진,Kwon, Young-Hyuk,Park, Joon-Bong,Herr, Yeek,Chung, Chong-Pyung,Han, Soo-Bog,Lee, Seung-Jin The Korean Academy of Periodontoloy 1998 Journal of Periodontal & Implant Science Vol.28 No.3

4 beagle dogs aged over one and half years and weighed 15 to 16 Kg were utilized in this study. Experimental dehiscent defects were made in the mandibular edentulous area after removal of lower premolar. e-PTFE membrane resorbable membrane, and PDGF-BB-loaded resorbable membrane were covered at the dehiscent defects around the dental implants respectively. Animal was sacrificed at 1, 2, 3 months respectively. Non-decalicifed specimens were made and mutiple-stained for light microscopic study. The results were as follows: 1. Dehiscent defects around the implant installed in the beagle dog were an excellent defect model for studying guided bone regeneration. 2. Fibroblasts penetrated into expanded-PTFE membrane was observed and inflammatory cell infiltration was also observed around the membrane. 3. Resorbable membrane was degradaded and resorbed at 1 month after application to the dehiscent defect. Though multinucleated giant cells were observed adjacent to the membrane, that had no reverse effect on the boe regeneration. 4. PDGF-BB-Ioaded resorbable membrane was same capability as the resorbable membrane and e-PTFE membrane in the guided bone regeneration. 5. PDGF-BB-Ioaded resorbable membrane-applied site was better than resorbable membrane-applied site in the speed and maturity of bone formation. Within the above results, it was suggested that PDGF-BB-Ioaded resorbable membrane might have same bone regeneration capacity as nonresorbable membrane in the dehisced implant of the beagle dog and potentiality to use in human subjects.

치근면 활택술후 2% minocycline gel의 치료효과

권영혁,박지원,허익,박준봉,Kwon, Young-Hyuk,Park, Ji-Won,Herr, Yeek,Park, Joon-Bong 대한치주과학회 1998 Journal of Periodontal & Implant Science Vol.28 No.4

The objective of the present study was to evaluate the clinical and microbiological effects of scaling and root planing combined with local application of 2% minocycline gel to patients with moderate to advanced chronic adult periodontitis. 27 healthy patients with moderate to advanced chronic adult periodontitis were enrolled in the study. The quadrants that had 2 or more teeth with $5{\sim}8mm$ probing pocket depth and radiographic evidence of alveolar bone loss were selected and divided into test side and control side according to the splitmouth design. All patients received standardized oral hygiene instructions at the beginning of the study. Subsequently scaling and root plaining was performed on all remaining teeth until 0 week. The 2% minocycline gel was applied to periodontal pocket at 0, 1, 2, 3week in the test side. The normal saline was irrigated subgingivally for about 30 seconds in the control side. The clinical and microbiological analysis was carried out at 0, 4, 8, and 12weeks. The results of this st udy were as follows; 1.2% minocycline gel delivered subgingivally as an adjunct to scaling and root planing provided benefit in reducing sulcular bleeding index and pocket depth than the use of normal saline. 2. The relative proportion of cocci and non-motile bacteria was increased in the test and control groups with time, and there was no statistically significantdifference between two groups. 3. The proportion of spirochetes was slowly reduced in the control group, but, inthe test group, they were remarkably reduced from the 4th week, and there was a statistically significant difference between two groups. 4. In both groups, the relative proportion of motile rods was notably decreasedat the beginning of the study, and remained until 12th week in the test group,but, in the control group, they were slowly increased from the 4th weekand finally similar to that of the initial examination. In conclusion, local application of 2% minocycline gel may be effective in the clinical and microbiological aspects as an adjunct to scaling and root planing in periodontal disease sites.

치주수술후 Zea Mays L. 투여가 치유과정에 미치는 영향에 대한 임상적 연구

권영혁,이만섭,양승한,김영,박준봉,Kwon, Young-Hyuk,Lee, Man-Sup,Yang, Seung-Han,Kim, Young,Park, Joon-Bong 대한치주과학회 1994 Journal of Periodontal & Implant Science Vol.24 No.3

The purpose of this study was to evaluate the effects of administration of Zea Mays L. on the healing process after periodontal surgery as adjuntives. Authors used 3 kinds of different clinical criteria, depth of periodontal pocket by using the Goldman Fox periodontal probe, degree, of tooth mobility by Periotest, and amount of occlusal force with electronic device. In this comparative clinical study, 30 patients who were divided into two group, 15 ZML administrated group and 15 placebo adminstrated group, were participated. All the examined teeth were isolated with gauze and air spray, and measured each clinical critera on the day of before surgery, 1, 2, 4, 8 weeks after surgery. The results were as follows. 1. The changes of the periodontal pocket depth, on the both of Zea Mays L. administrated group and placebo adminstrated group, revealed the decreasing tendency, and it was shown the time dependent tendency. But there was no statistically significant differences between the two group. 2. In the case of tooth mobility, both group showed the highest severe mobility on the 1 week after surgery. It was observed that experimental group had more effects on decreasing the mobility. But there was no statistically significant differences between the two group. 3. In the case of experimental group, the recovery trend of occlusal forces after periodontal surgery on the molar teeth revealed higher than the control group. But there was no statistically significant differences between the two group. In conclusion, Zea Mays L. may play a favorable role in the healing process after periodontal surgery. It was suggested that further study to evaluate the effects of selective administration on the patient who have systemic diseases should be needed.

중증치주염환자의 T 및 B 임파구와 T-subsets의 분포에 관한 연구

권영혁,Kwon, Young-Hyuk 대한치주과학회 1982 Journal of Periodontal & Implant Science Vol.12 No.1

램프미터링 운영기준 정립에 관한 연구 : 서울외곽순환고속도로 사례 중심으로

권영혁(Kwon,Young Hyuk),윤재용(Yoon,Jae Yong),이의은(Lee,Eui Eun) 대한토목학회 2011 대한토목학회논문집 D Vol.31 No.5D

교통수요의 증가와 이용행태는 도로 건설로만 감당하기에는 한계에 도달했고 기존 시스템의 최대 활용을 도모함으로써 그 해결방안을 찾고자 하는 노력이 다방면에서 검토·연구되고 있는데 그 중 가장 대표적인 전략이 연결로 진입교통량을 통제 하는 램프미터링 시스템(RMS)이다. 현재 운영되고 있는 서울외곽순환고속도로 장수~중동 구간 운영기준을 고찰하고, 지리적 및 교통상황을 고려한 운영기준을 재정립하였다. 이것을 위해 국내 및 해외 램프미터링 운영기준을 분석하였고, 단절점 분석으로 새로운 램프미터링 운영기준을 제시하였다. The increase in the traffic demand and behavioral pattern of its use reached the limit for the only means of road construction to bear; thus the multilateral efforts to find a solution to this problem together with the examination and research on this issue, among which the most representative strategy is Ramp Metering System controlling access traffic volume to connection roads. The operation standard and running time was re-established in consideration of geographical conditions and traffic circumstances by considering the standard of operation and running time of the currently operated Jangsu- Jungdong section of the outer ring expressway of Seoul. For this purpose, this research analyzed the operative standard of RMS home and abroad and suggested a new operative standard of ramp metering through the analysis of break points.

흡수성 차폐막을 이용한 조직유도재생술의 임상적 효과

허인식,권영혁,이만섭,박준봉,허익,Hur, Yin-Shik,Kwon, Young-Hyuk,Lee, Man-Sup,Park, Joon-Bong,Herr, Yeek 대한치주과학회 1999 Journal of Periodontal & Implant Science Vol.29 No.1

The purpose of this study was to compare the clinical results of guided tissue regeneration(GTR) using a resorbable barrier manufactured from an copolymer of polylactic acid (PLA) and polylaetic-glycolic acid(PLGA) with those of nonresorbable ePTFE barrier. Thirty two patients(25 to 59 years old) with one radiographically evident intrabony lesion of probing depth ${\geq}$6mm participated in a 6-month controlled clinical trial. The subjects were randomly divided into three independent groups. The first group(n=8) received a ePTFE barrier. The second group (n=12) received a resorbable PLA/PLGA barrier. The third group (n=12) received a resorbable PLA/PLGA barrier combined with an alloplastic bone graft. Plaque index (PI), gingival index(GI), probing depth(PD), gingival recession, clinical attachment level(CAL), and tooth mobility were recorded prior to surgery and at 3, 6 months postsurgery, Statistical tests used to analyze these data included independent t-test, paired t-test, one-way ANOVA. The results were as follows : 1. Probing depth was significantly reduced in all groups at 3, 6 months postsurgery and there were not significant differences between groups. 2. Clinical attachment level was significantly increased in all groups at 3, 6 months postsurgery and there were not significant differences between groups. 3. There were not significant differences in probing depth, clinical attachment level, gingival recession, tooth mobility between second group (PLA/PLGA barrier) and third group (PLA/PLGA barrier combined with alloplastic bone graft) 4. Tooth mobility was not significantly increased in all groups at 3, 6 months postsurgery and there were not significant differences between groups. In conclusion, PLA/PLGA resorbable barrier has similar clinical potential to eP'IFE barrier in GTR procedure of intrabony pockets under the present protocol.

핀 고정 천공형 티타늄막을 이용한 수직적 체조제증대술에 관한 연구

김영,권영혁,박준봉,허익,정종혁,Kim, Young,Kwon, Young-Hyuk,Park, Joon-Bong,Herr, Yeek,Chung, Jong-Hyuk 대한치주과학회 2006 Journal of Periodontal & Implant Science Vol.36 No.2

The purpose of this study was to evaluate exophytically vertical bone formation in rabbit calvaria by the concept of guided bone regeneration with a custom-made porous titanium membrane combined with bone graft materials. For this purpose, a total of 12 rabbits were used, and decorticated calvaria were created with round carbide bur to promote bleeding and blood clot formation in the wound area. Porous titanium membranes (0.5 mm in pore diameter, 10 mm in one side, 2 mm in inner height) were placed on the decorticated calvaria, fixed with metal pins and covered with full-thickness flap. Experimental group I was treated as titanium membrane only. Experimental group II, III, IV was treated as titanium membrane with BBM, titanium membrane with DFDB and titanium membrane with FDB. The animals were sacrificed at 8 and 12 weeks after surgery, and new bone formation was assessed by histomorphometric as well as statistical analysis. 1. Porous titanium membrane was biocompatable and capable of maintaining the regeneration space. 2. At 8 and 12 weeks, all groups demonstrated exophytic bone formation and there was a statistical significant difference among different groups only at 12 weeks. 3. The DFDB group revealed the most new bone formation compared to other groups (p<0.05). 4. At 12 weeks, DFDB and FDB groups showed the most significant resorption of graft materials (p<0.05). 5. The BBM was not resorbed at all until 12 weeks. 6. The fixation metal pin revealed excellent effect in peripheral sealing. On the basis of these findings, we conclude that a porous titanium membrane may be used as an augmentation membrane for guided bone regeneration, and DFDB as an effective bone forming graft material. The fixation of the membrane with pin will be helpful in GBR technique. However, further study is required to examine their efficacy in the intraoral experiments.

골유도재생술시 그물형 티타늄막의 천공수가 골형성에 미치는 영향

이근혁,권영혁,박준봉,허익,Lee, Keun-Hyuk,Kwon, Young-Hyuk,Park, Joon-Bong,Herr, Yeek 대한치주과학회 2004 Journal of Periodontal & Implant Science Vol.34 No.2

This study was performed to evaluate bone formation in the calvaria of rabbit by the concept of guided bone regeneration with titanium mesh membrane. Two different titanium meshes with varying number (353, 565) of pore were utilized in the study. Two surgical sites(T353, T565) were evaluated about whether or not the number of pore may have effect on the bone formation. The animal was sacrificed at 10days, 3 weeks, 6weeks, and 8 weeks after the surgery. Non-decalcified specimens were processed for histologic analysis. 1. Titanium mesh was biocompatible and capable of maintaining the spacemaking. 2. At 3 weeks, 6 weeks, and 8 weeks after GBR procedure, bone formation was more in the T353 site than in the T565 site. 3. Soft tissue layer above the regenerated bone was better developed in the T565 site. 4. There was no difference between two membranes in bone maturity with time. Within the above results, titanium mesh with lesser pore in number might be recommended for the early bone formation.