움직임 벡터를 찾기위한 혼합 탐색 방법

고영주(Young-Joo Koh),양성봉(Sung-Bong Yang) 한국정보과학회 1999 한국정보과학회 학술발표논문집 Vol.26 No.1B

움직임벡터의 탐색 시, 한 프레임 내에서도 배경과 같은 부분은 움직임이 없고, 하나의 객체는 같은 방향으로 움직일 것이며, 경계선에서는 연관성이 없을 것이다. 이러한 특성들을 고려할 때, 프레임 내에서 움직임벡터의 탐색을 위해 하나의 알고리즘을 사용하는 것은 비효율적일 수 있다. 이 때 움직임벡터의 연관성을 분석해서 적당한 임계값을 부여한 후 각 블록에 알맞은 알고리즘을 적용시킨다면 움직임벡터 타색의 효율을 향상시킬 수 있다. 현재 블록의 움직임벡터를 구하기 위해, 수직 연관성과 수평연관성을 가지는 각 블록들의 움직임벡터를 분석하여 한 프레임 내에서 여러 알고리즘을 적용하는 것이 혼합 탐색방법의 기본 아이디어이다. 분석하고자 하는 두 블록의 움직임벡터들의 차이가 적다면 연관성이 많다고 판단, 이들의 평균값으로 중심좌표를 옮겨서 알고리즘을 적용하고, 차이가 클수록 그 특성에 알맞은 방법들을 적용시키는 것이다. 수직성분과 수평성분의 차이가 크면 연관성이 적다고 판단, 그 성질에 알맞은 탐색 알고리즘을 사용하기 때문에 비용과 효율 면에서 상당한 효과를 얻을 수 있었다.

쾌속조형기법을 이용한 생분해성 스텐트용 메쉬필름의 약물방출거동 효과

정신영(Sin Young Cheong),김양은(Yang Eun Kim),고영주(Young Joo Koh),신왕수(Wang Soo Shin),이준희(Jun Hee Lee),김완두(Wan Doo Kim),유영은(Young Eun Yoo),박수아(Su A Park) 한국고분자학회 2014 폴리머 Vol.38 No.1

약물방출 고분자 코팅 스텐트는 수술후 재협착을 획기적으로 줄였지만, 약물방출이 균일한 구조체를 제작하는 것이 어렵고 체내에 구조체를 영구적으로 남겨야 하는 부담을 여전히 가지고 있다. 이를 해결하는 방안으로 생분해성 고분자로 스텐트를 제작하는 방법들이 활발하게 연구되고 있다. 본 연구에서는 조형가공기술(solid freeformfabrication, SFF)의 하나인 쾌속조형기법(rapid prototyping technique)의 3차원 플로팅(3D plotting) 기술을 이용하여파크리탁셀(PTX) 약물을 함유한 폴리카프로락톤(PCL) 3차원 구조체를 제작하였고, 생분해성 PCL 고분자로부터PTX의 방출거동과 스텐트 제작 가능성을 고찰하였다. 약물을 포함한 구조체의 표면특성을 SEM으로 확인한 결과굴곡이 자연스럽고 매끄러운 표면을 가지고 있었다. FTIR을 통해서 약물이 성공적으로 구조체에 포함되었음을 확인하였고, NMR과 HPLC를 통해서 PCL 구조체 중의 PCL함량과 PTX의 서서히 방출됨을 확인되었다. 또한 세포실험을 통해 구조체에서 방출된 약물이 생물학적으로 활성을 유지하고 있으며, 반복제작된 구조체에서도 균일한 활성의 약물이 방출됨을 확인하였다. 이와 같은 쾌속조형기법을 이용하여 약물을 포함하는 구조체를 제작하고 분석함으로써, 생분해성 고분자 스텐트로서의 적용가능성을 제시하였다. Biodegradable drug-eluting stent has dual functions of supporting the lumen and treating internal tumor preventingthe restenosis by releasing drug. In this study, the polycaprolactone (PCL) based three dimensional (3D) meshloaded with paclitaxel (PTX) was presented by rapid prototyping (RP) technique of solid freeform fabrication (SFF) forbiodegradable drug-eluting stent application. PCL has many advantageous properties such as good biocompatibility, goodmechanical properties, and good drug permeability. PTX is widely used in the cancer treatment by inhibiting tumor cellproliferation. Analytical methods of HPLC and NMR were used for simultaneous quantification of PTX. Scanning electronmicroscopy (SEM) was performed to observe the architecture and morphologies of 3D mesh. The cytotoxicity assayresults indicated released PTX`s biological activity. This study provided that PCL based 3D mesh loaded with PTX byRP technique has great potential for biodegradable drug-eluting stent application.

Poly(p-dioxanone)(PPDO)의 물리적 특성과 모노필라멘트 봉합사의 In vitro 가수분해 특성

이준희,고석연,고영주,신재섭,정용석,권수한,Lee, June-Hee,Ko, Suk-Yen,Koh, Young-Joo,Shin, Jae-Sup,Chung, Yong-Seog,Kwon, Soo-Han 한국섬유공학회 2010 한국섬유공학회지 Vol.47 No.4

The bulk polymerization and thermal properties of biodegradable poly(1,4-dioxan-2-one)[poly(p-dioxanone), PPDO] were investigated. The in vitro hydrolytic degradation behavior of PPDO monofilament sutures were also examined over a 16 week period at $37^{\circ}C$ and $45^{\circ}C$. The degree of PPDO polymerization decreased with increasing content of the initiator, lauryl alcohol[$CH_3(CH_2)_{11}OH$]. The thermal stability of PPDO was inversely proportional to the content of the catalyst, Sn(II) ethylhexanoate[$Sn(Oct)_2$]. Considering the efficiency of polymerization and the thermal stability of PPDO, the proper contents of Lauryl alcohol as an initiator and ethylhexanoate as a catalyst were 1600~1900 ppm and 20~30 ppm, respectively. Based on the determination of the half crystallization time, the crystallization speeds of dyed PPDO chip and monofilament suture were higher than those of undyed PPDO chip and monofilament suture. The maximum rates of crystallization of the dyed and undyed samples were observed at around $40{\sim}50^{\circ}C$ and $50{\sim}60^{\circ}C$, respectively. At the beginning of the in vitro time, the tensile strength of the PPDO monofilament suture decreased slowly but decreased considerably after a certain period, indicating that the degradation proceeded in two steps, the first occurring in the amorphous regions and the second in the crystalline regions. The average molecular weight of the PPDO monofilament suture decreased continuously from the beginning of the in vitro time, due to the random nature of the degradation process. The breaking strength retention (BSR) and molecular weight of the PPDO monofilament suture showed a quadratic function relationship.

자동화 공정을 사용한 I 형상/Y 형상/dual pore tubular scaffold 제작

조용상(Yong Sang Cho),고영주(Young-Joo Koh),최기평(Ghi Pyoung Choi),우창희(Chang Hee Woo),안상현(Sang Hyun An),예대희(dae Hee Yeh),김호(ho kim),이윤행(Yoon haeng Lee),조영삼(Young-Sam Cho) 대한기계학회 2011 대한기계학회 춘추학술대회 Vol.2011 No.10

유아의 기질과 기초체력과의 관계

진행미 ( Haeng Mi Jin ),고영주 ( Young Joo Koh ) 한국유아체육학회 2008 한국유아체육학회지 Vol.9 No.2

The purpose of this study was to suggest the child`s temperament is a factor on the physical fitness of the individual. This study will furnish basic bata available for teaching children to develop their physical abilities by examining the relation between the ages of 4 to 6 in D. L kindergartens, located in E, G ward of Seoul. The Instrument of Children Temperament(KOH Tea-Soon, 1997) was used as a measurement tool for temperament and The Test Of Motor Ability(OH Jin-Koo and 2) was to test physical fitness. SPSS(ver 10.0) was used for the analysis of data, t-test was used to not ify the sexual difference of temperament and physical fitness, Pearson`s correlation coefficient was used for analysing the relation between the subfactor of temperament and physical fitness. The result of this study is as follows. First, the difference(p<.05) of temperament according to the sex appeared in the adaptability and activity levels. Additionally, it was found out that male children are more adaptable to new and changeful environment and more able to adapt to new and changing environments and more physically active than female children. Second, according to the differences between male and female children in physical fitness. females scored higher than males in flexibility(p<.01), coordination(p<.001) and males scored higher than femailes in balance(p<.05). Third, the correlation between temperament and muscular endurance appeared to be positive correlation in distractibility and flexibility and negative correlation in regularity and coordination.

반월상 연골 재생을 위한 친수화 처리 폴리다이옥사논 지지체의 제조 및 분석

조광준 ( Kwang Joon Cho ),송대근 ( Dae Kun Song ),오세행 ( Se Heang Oh ),고영주 ( Young Joo Koh ),이상훈 ( Sahng Hoon Lee ),이명철 ( Myung Chul Lee ),이진호 ( Jin Ho Lee ) 한국조직공학과 재생의학회 2005 조직공학과 재생의학 Vol.2 No.2

Biodegradable polymers have been widely used to fabricate porous scaffolds for three-dimensional (3-D) cell cultures to regenerate tissue-based artificial organs. Until now, poly(L-lactic acid), poly(glycolic acid), and poly(lactic-co-glycolic acid) as synthetic biodegradable polymers have been most widely used to fabricate 3-D scaffolds. However, these polymers have limitations for some applications because of their brittleness, hydrophobicity, and low cell adhesiveness. Recently polydioxanone (PDO) has been interested in the applications for tissue regeneration since this polymer has good elasticity as well as biocompatibility and biodegradability. However, porous scaffold fabrications from PDO were limited due to its poor solubility (solvents to dissolve PDO are few). In this study, we firstly fabricated porous PDO scaffolds (disc and meniscus shapes) using a melt-molding particulate-leaching method developed by our laboratory. This method does not need any solvents during the fabrication process. To improve the hydrophilicity and cell compatibility of the scaffolds, PDO was blended with polyvinyl alcohol (PVA) with different ratio. The hydrophilicity, morphology, porosity, and mechanical properties of the prepared PDO scaffolds were investigated. The PDO/PVA (5 wt%) blend scaffold was found to have sufficient hydrophilicity, mechanical strength, and elasticity for tissue engineering applications including meniscus regeneration.

Monascus purpureus를 이용한 쌀로 부터 천연식용 색소생산에 관한 연구

변광의,장성근,고영주 순천향대학교 기초과학연구소 1995 순천향자연과학연구 논문집 Vol.1 No.1

Studies on the optimal conditions for the production of natural edible red pigments with Monascus purpureus Went were performed. The optimal pH of strain on agar medium was 5.5 and the optimum autoclaving time for rice medium was twenty minutes. Analysis of food components of the raw or the fermented rice showed a remarkable changes: the contents of components were increased in moisture, crude protein and crude fat after the fermentation, but decreased in starch. starch. There was no difference in ash content but the reducing sugar in the fermented rice was detectable. The color intensity of the fermented rice revealed a wide range of differences among the regions produced. The water activity of rice medium was decreased but colony counts were increased during the fermentation periods. In the ergosterol assay, the peaks of ergosterol standard and fermented rice revealed a similar retention time. Changes in food components during the fermentation periods were daily investigated: the contents in moisture, crude protein and reducing sugar were increased but in starch content decreased. The water soluble extracellular pigment and the alcohol soluble intracellular pigment were increased continuously but the water soluble total pigment decreased at 10 day. In effect of inorganic salts, (NH₄)₂SO₄. FeSO₄·7H₂O. NH₄NO₃. MnO₂. NaN₃ and KNO₃have stimulated the pigment production. The absorption spectra of pigment solutions exhibited the maximum at 400nm and 500nm On thin layer chromatogram, the red pigment preparation revealed five fractions {R₁(??) -R₄} and yellow pigment preparation consisted of single fraction(Y₁). These basic results will be applied for studies of the polyketide biosynthesis pathway, the innovation of solid-state bioreactor and the production of natural food ingredients.

검정곰팡이 (Aspergillus niger) 의 포자형성에 미치는 cAMP , 테오필린 및 카페인의 영향에 관한 연구

김종협,고영주 한국균학회 1987 韓國菌學會誌 Vol.15 No.4

Aspergillus niger van Tieghem was cultured by the method of synchronous and submerged culture. The sporulation occurred through the culture and its life cycle and differentiation were completed in the experiments. The effects of cAMP, theophylline and caffeine on the sporulation of A. niger were investigated. In the sporulation medium, the sporulation was stimulated by addition of CAMP and its optimum concentration was 10^(-4)M. In the sporulation medium, the sporulation was stimulated by addition of theophylline and its optimum concentration was 10 ㎎/㎖. In the sporulation medium, the sporulation was stimulated by addition of caffeine and its optimum concentration was 300 ㎎/㎖. Theophylline added to the sporulation medium together with CAMP enhanced the promotion effect of cAMP on sporulation. Caffeine added to the sporulation medium together with cAMP enhanced the promotion effect of CAMP on sporulation. In the sporulation medium, the sporulation was stimulated by addition of neither AMP nor ATP. In the potassium acetate medium, cAMP, theophylline and caffeine stimulated the sporulation, respectively.