로직모델을 이용한 경기도일자리재단의 4050재취업지원사업 시범 성과평가

강혜라 한국사회복지행정학회 2018 한국사회복지행정학회 학술대회 자료집 Vol.2018 No.4

학생기록물에 대한 대학생의 인식 유형 연구 - 전남대학교를 중심으로 -

강혜라,장우권,Kang, Hye-Ra,Chang, Woo-Kwon 한국비블리아학회 2017 한국비블리아학회지 Vol.28 No.3

이 연구는 학생기록물의 효과적인 관리를 위해 주체적 생산자인 대학생의 인식에 대한 심층적인 연구가 필요함을 제기하고 Q 방법론을 통해 학생기록물의 중요도에 관한 주관적인 인식(Q1)유형과 학생기록물에 관한 주관적인 인식(Q2) 유형을 도출하는데 목적이 있다. 이를 위해 전남대학교의 학생을 대상으로 학년별로 8명씩 설문과 인터뷰를 진행한 결과, Q1 유형은 집단적 증거형, 주체적 활동형, 민주적 증거형, 성취 중시형이 도출되었으며, Q2 유형은 공동체 역사적 가치형, 개인적 소유형, 역사적 전문가 관리형, 역사적 활용 가치형, 비역사적 보존형이 도출되었다. 연구결과를 바탕으로 첫째, 다양한 기록물 속성을 포괄할 수 있는 관점에서 학생기록물의 관리방안의 수립이 필요하다는 점과 둘째, 공동체 아카이브의 관점에서 학생기록물의 관리방안이 수립되어야 한다는 점, 셋째, 학생들이 생산한 학생기록물의 보존을 위한 전략적인 방안(학생기록물의 생애주기를 고려한 보존을 위한 캠페인 등)이 필요함을 제안했다. This study brings up that further studies on the perceptions of university students should be conducted for efficient management of student records. Moreover, the Q methodology is used to identify subjective recognition (Q1) type related to the importance of student records and subjective perception (Q2) of student records type. For this purpose, I carried out questionnaires and interviews with eight students by each grade at Chonnam National University. As a result, Q1 type was derived from Collective Evidence type, Independent activity type, Democratic witness type, and achievement-oriented type. The type of Q2 is derived from Historical Value of Community Type, Personal Possession Type, Historical Expert Management Type, Value for Historical Practical Use Type, and Non-historical Preservation Type. Based on the research results, the following are suggested: First, the management of student records is needed in a way that encompasses various records of various records properties. Second, the management of student records should be established from the perspective of the Community Archives. Third, a strategic measure for the preservation of student records produced by students is necessary (e.g., a campaign for conservation of student records).

기록물관리와 정보공개의 상관성에 관한 연구: 지방자치단체의 정보공개 자치법규를 중심으로

강혜라,장우권 한국정보관리학회 2016 정보관리학회지 Vol.33 No.4

이 연구는 지자체의 자치법규에서 정보공개제도와 기록물관리의 상관성을 규명하기 위한 것이다. 이를 위해 자치법규정보시스템(ELIS)과 국가법령정보센터를 통해 지자체의 정보공개와 관련된 자치법규를 전수 조사하여, 337개의 자치법규를 분석하였다. 그 결과, ‘기록 유지(회의록 작성)’, ‘이관된 기록물에 대한 정보공개 절차’와 ‘청구 접수부서’의 측면에서 ‘공공기록물법’과의 상관성을 찾을 수 있었다. ‘기록물유지(회의록 작성)’는 자치법규와 공공기록물법의 항목이 유사했으며, ‘청구 접수부서’는 ‘기록관리부서’로 기술되었다. 그러나 ‘청구 접수부서’는 부서명만 보면 민원부서의 성격이 강했으며, ‘회의록 작성’의 측면에서는 ‘공공기록물법’의 조항이 명시되지 않았고, ‘다른 법령과의 관계’에서는 ‘정보공개의 비대상’만 기술하고 있다. This study aims to shed light on the relationship between information official disclosure and records management in the local government’s authority regulations. To that end, analyzed 337 local autonomy laws found in ELIS and the Ministry of Office of Legislation. As a result, it found a link between the ‘Public Records Act’ in terms of ‘records preservation (making minutes)’, ‘information disclosure procedures for transferred records,’ and claims receiving department. ‘Record keeping (written minutes)’ was similar to that of the ‘Public Records Act,’ and the ‘Claims Receiving Department’ mentioned ‘Record Management Department.’ However, the ‘Claims Reception Department’ had a strong characteristic of the civil service department, and the ‘Public Record Act’ did not specify the clause in terms of ‘minutes of the minutes.’ In ‘relation with other laws.’

해외자원봉사활동이 대학생의 세계시민의식과 다문화수용성에 미치는 영향

강혜라,홍영준 한국시민청소년학회 2015 시민청소년학연구 Vol.6 No.2

본 연구는 대학생의 해외자원봉사활동의 효과성을 검증하고 해외자원봉사활 동의 발전방향을 논의하기 위하여 대학생들의 세계시민의식과 다문화수용성을 조사하고 해외자원봉사활동 특성에 따른 이들의 차이를 분석하였다. 대학생들 의 해외자원봉사활동에 따른 각 변수들의 차이를 분석한 결과는 다음과 같다. 첫째, 해외자원봉사활동 전과 후의 다문화수용성의 경우에는 통계적으로 유 의미한 차이를 보인 반면 세계시민의식은 유의미한 변화가 나타나지 않았다. 둘째, 해외자원봉사활동의 기간 및 시간에 따른 차이는 나타나지 않았으나, 현 지인과의 교류 여부에 따라 다문화수용성의 유의미한 차이가 있는 것으로 분 석되었다. 셋째, 인구사회학적 변인 중 성별, 학년, 전공은 세계시민의식과 다 문화수용성에 영향을 미치는 것으로 나타났다. 본 연구의 결과는 대학생의 해 외자원봉사활동 전과 후의 다문화수용성의 차이를 입증함으로써 그 효과성에 관한 실증적 연구를 하였다는 점에서 의의를 찾을 수 있다.

Malignancy Rates of Suspicious Breast Lesions in Patients on Annual Screening or Regular Follow-up Ultrasonography

강혜라,김성헌,강봉주,최병길 대한초음파의학회 2013 ULTRASONOGRAPHY Vol.32 No.3

Purpose: The objective of this study is to investigate the malignancy rates of BIRADS 4 and 5 lesions detected on annual screening or follow-up US and to correlate the malignancy rates with the rationales for biopsy. Materials and Methods: Between 2010 and 2011, among 2837 patients who underwent US-guided core-needle biopsy, 423 patients underwent screening or regular follow-up US examinations. Lesions were classified into four groups: newly detected suspicious lesions, benign lesions with interval growth, benign lesions with suspicious changes of US features and benign lesions with interval growth and suspicious changes of US features. Results: The malignancy rates were as follows: BI-RADS 4A low suspicion of malignancy, 8.1% (31/379); 4B intermediate suspicion of malignancy, 56% (14/25);4C moderate suspicion of malignancy, 71.4% (10/14); 5 highly suggestive of malignancy,100% (5/5). The malignancy rates for the four groups were as follows: newly detected suspicious lesions, 17.46% (51/292); benign lesions with interval growth,7.77% (7/90); benign lesions with suspicious changes of US features, 4% (1/25);benign lesions with interval growth and suspicious changes of US features, 6.25%(1/16). Comparison of the malignancy rate of newly detected suspicious lesions with that of benign lesions with interval growth revealed a statistically significant difference (p=0.038). No significant differences were observed between the other groups (p>0.05). Conclusions: The malignancy rates of suspicious lesions detected on annual screening or follow-up US were similar to those of the reported BI-RADS data. A higher malignancy rate was observed for newly detected suspicious lesions than for lesions with interval growth.

CHO 세포 배양에서 미토콘드리아 생합성 유도를 통한 재조합 단백질 생산성 증대

강혜임,백종윤 한국생물공학회 2022 KSBB Journal Vol.37 No.4

Mitochondria are the powerhouse of a cell that produces cellular energy, especially adenosine triphosphate (ATP), to fuel cell biosynthesis and protein production. In this study, we aim at inducing mitochondrial biogenesis in Chinese hamster ovary (CHO) cells to increase cellular ATP levels as well as recombinant protein productivity. Focusing on the culture process engineering, we tested two chemical additives, resveratrol and nicotinamide adenine dinucleotide (NAD), in CHO cell cultures to reinforce mitochondrial biogenesis. Resveratrol and NAD treatment resulted in an increase in specific productivity (q p ) by 10-30% and 40-50% compared to the control, respectively. In addition, NAD exhibited an increase in viable cell densities, culture longevity and specific lactate uptake rates. Moreover, along with an increase in the ATP level, mitochondrial DNA (mtDNA) copy number and mRNA expression of the mitochondrial biogenesis-related genes were also up-regulated compared to the control. In conclusion, although there might be varied cell line-specific impacts on cell growth, inducing mitochondrial biogenesis using chemical additives would be an effective strategy for improving recombinant proteins productivity in CHO cells culture.

Vero Cell과의 공동배양이 체외에서 생쥐 난자성숙과 배아발생에 미치는 영향

강혜나,하종식 梨花女子大學校 醫科大學 醫科學硏究所 1996 EMJ (Ewha medical journal) Vol.19 No.3

Assisted reproductive technology(ART) have contributed significantly to alleviating subfer-tility in the childless couple. In spite of the many advances in the field of ART, the pregnancy and take-home baby rates for in vitro fertilization(IVF) have been very poor. In order to overcome these problems, a variety of coculture systems has been devised. Vero cells, derived from African green monkey kidney, were selected because kidney and genital tract have a common embryonic origin. In addition, these cells are safe for coculture with embryos : they are highly controlled for viruses and other contaminants because they are used for vaccine production. Several investigators showed that cocultureing human embryos with Vero cells in vitro resulted in an improvement of embryo development. However, they did not observe the same results using mouse oocytes and embryos. We thus designed a series of experiments to demonstrate whether or not Vero cells do indeed enhance mouse oocyte maturation and embryo development. In this experiment, Vero cell does not allow the mouse immature oocytes to be enhanced maturation rate in vitro. To study the 'In-Vitro 2-cell Block' in mouse embryo, we have cocultured ICR one-cell mouse embryos with Vero cell in different medium. In Ham's F-10 the mouse embryos arrested their development prior to 4-cell stage(control 76.7%;coculture 75.0%). In contrast, the coculturing mouse embryos revealed enhanced development(control 0%;coculture 22.8%) in human tubal fluid(HTF) only in late embryonic stages(hatching). On the other hand, the degree of blastomere fragmentation exhibited a reverse trend to that of the developmental capacity. Embryos from coculture groups(Ham's F-10 & HTF) showed some fragmentation(0% & 4.2%) while 13.3% and 14.3% of the embryos in control groups(Ham's F-10 & HTF) were severely fragmented(P<0.05). Thus the use of coculture systems appears to be dependent on the type of medium used as a support. The development rate of late 2-cell mouse embryos in Vero cell coculture was no significant.

北宗禪硏究 - 頓漸辨證法的 止揚

姜慧? 東國大學校 釋林會 1976 석림 Vol.10 No.-

Vero Cell-Conditioned Medium에서의 배양이 생쥐 후기 2-세포기 배아 발생에 미치는 영향

강혜나,하종식 梨花女子大學校 醫科大學 醫科學硏究所 1996 EMJ (Ewha medical journal) Vol.19 No.4

본 실험결과 Vero cell과의 공동배양과 Vero cell-conditioned medium에서의 배양은 생쥐 후기 2-세포기 배아의 부화율을 놓이고 세편이 적은 양질의 배아획득을 돕고 세포 증식을 빠르게 해 팽윤 배반포시 할구수를 증가시키는 것으로 나타났다. 그러나 앞으로 공동배양 기작에 작용할 것으로 보이는 embryotrophic factor나 배양액에서 공동배양 세포주에의해 제거되는 발생저해 물질 등의 분자생물학적 연구가 진행되어야하고, 수정시 정자등이 공동배양 세포주의 monolayer에 결합하여 수정 기회를 감소시켜 여러 장점에도 불구하고 오히려 수정률을 감소시킬 수 있는 적접적인 공동배양보다 conditioned medium에서의 배양에 관심을 가져야할 것으로 본다. This study was conducted to evaluate the ability of Vero cell-conditioned medium for supporting mouse embryo development in vitro. The mouse late 2-cell embryos were cultured in control media(Ham's F-10 +10%FBS), media with Vero cell monolayer and Vero cell-conditioned media for 4 days and measured the hatching rate and cell number in the blastocyst stage. The hatching rate in experimental groups was increased significantly compared with embryos in control group(p<0.01). On the other hand, the degree of blastomere fragmentaion exhibited a opposite trend to that of the developmental capacity(p<0.05). And also the cell numbers of expanded blastocysts in experimental groups were increased significantly compared with the control group(p<0.001). There was, however, no difference between experimental groups. These results indicate that Vero cell-conditioned medium supported the mouse embryo development as a Vero cell monolayer. And the mechanism for enhancement of the development potential of embryos may be releasing the embryotrophic factor during the medium-conditioning period.

Vero Cell과의 공동배양이 체외에서 생쥐 난자성숙과 배아발생에 미치는 영향

강혜나,하종식 이화여자대학교 생명과학연구소 1996 생명과학연구논문집 Vol.7 No.-

Assisted reproductive technology(ART)have contributed significantly to alleviating subfertility in the childless couple. In spite of the many advances in the field of ART, the pregnacy and take-home baby rates for in virto fertilization(IVF)have been very poor. In order to overcome these problems, a variety of coculture systems has been devised. Vero cells, derived from African green monkey kidney, were selected because kidney and genital tract have a common embryonic origin. In addition, these cells are safe for coculture with embryos:they are highly controlled for viruses and other contaminants because they are used for vaccine production. Several investigators showed that coculturing human embryos with Vero cells in vitro resulted in an improvement of embryo development. However, they did not observe the same results using mouse oocytes and embryos. We thus designed a series of experiments to demonstrate whether or not Vero cells do indeed enhance mouse oocyte maturation and embryo development. In this experiment, Vero cell does not allow the mouse immature oocytes to be enhanced maturation rate in vitro. To study the 'In-Vitro 2-Cell Block' in mouse embryo, we have cocultured ICR one-cell mouse embryos with Vero cell in different medium. In Ham's F-10 the mouse embryos arrested their development prior to 4-cell stage(control 76.7%:coculture 75.0%). In contrast, the coculturing mouse embryos revealed enhanced development(control 0%;coculture 22.8%) in human tubal fluid(HTF)only in late embryonic stages(hatching). On the other hand, the degree of blastomere fragmentation exhibited a reverse trend to that of the developmental capacity. Embryos from coculture groups(Ham's F-10 & HTF)showed some fragmentation(0% & 4.2%)while 13.3% and 14.3% of the embryos in control groups(Ham's F-10 & HTF)were severely fragmented (P<0.05). Thus the use of coculture systems appears to be dependent on the type of medium used as a support. The development rate of late 2-cell mouse embryos in Vero cell coculture was no significant differences until blastocyst stage but improved at late develpmental stage(control 42.1% : coculture 70.7%). Thus the Vero cell coculture system was shown to increase the hatching rate of mouse embryos.