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홍화씨 추출물이 치주인대세포와 조골유사세포의 골 광물화 작용에 미치는 효과
강정구,유형근,신형식,Kang, Jung-Goo,You, Hyung-Keun,Shin, Hyung-Shik 대한치주과학회 1998 Journal of Periodontal & Implant Science Vol.28 No.3
The main goal for the treatment of periodontal diseases is the regeneration of lost cementum, bone and connective tissue. Clinical and histological research suggests that it is possible to restore periodontal structures. Seeds of Carthamus tinctorius L. has been used for the treatment of bone fracture and osteoporosis in traditional Korean medicine. The purpose of this study is to examine the effect of extract of seeds of Carthamus tinctorius L. on mineralization in periodontal ligament cells and osteoblastic cells. Periodontal ligament cells were primarily obtained from a extracted premolars with non-periodontal diseases, Osteoblastic cells were obtained from calvariae of a fetal rat, Cells were cultured with DMEM at $37^{\circ}C$ with 5% $CO_2$ in 100% humidity incubator. Alkaline phosphatase(ALP) level and the number of calcification nodules were examined and western blot analysis using osteonectin was performed, Measurements of ALP levels and calcification nodules showed that extract of seeds of Carthamus tinctorius L. had significantly higher activity than control in all of both cells. In western blot analysis, protein expression of osteonectin indicated that extract of seeds of Carthamus tinctorius L. showed an increased pattern than control in all of both cells. From the above results, it seems that extract of seeds of Carthamus tinctorius L. has excellent effect on mineralization in periodontal ligament cells and osteoblastic cells.
혐기성 처리공법에 의한 분뇨와 주방폐기물의 혼합처리에 관한 연구
최의소,강정구,박후원 한국물환경학회 1991 한국물환경학회지 Vol.7 No.1
The high moisture and organic contents of food wastes from the residential sources are an obstacle factor for the waste disposal and energy recovery process such as incineration and pyrolysis. In addition, the anaerobic nightsoil treatment plants in this country are experienced with a decreasing waste load due to the rapid conversion toward the modern toilet system. In order to overcome these difficulties, the application of the anaerobic treatment using the mixture of nightsoil and food wastes would be an alternative treatment process. This study was therefore focused to determine design parameters for the anaerobic treatment for nightsoil and food waste mixture. The experimental results indicated that treatment efficiencies measured by VS and COD reductions, gas productions and gas portions were varied with organic loadings and mixture ratios of nightsoil to food waste.