癎疾患者에 있어서 toxoplasma 抗體價

殷洪培 전북대학교 의과학연구소 1982 全北醫大論文集 Vol.6 No.1

This study was undertaken to evaluate toxoplasma antibody prevalence by Sabin-Feldman dye-test in epileptics(n=51), schizophrenica(n=24), and normal persons(n=60). Fourfold serum dilutions were used, we considered tilters of 1:16 or more positive. The number and percentage of positive reaction in dye-test was shown as follows : 42(82.3%) of 51 epileptics, 13 (54.1%) of 60 normal persons. Toxoplasma antibody prevalence in epileptics was not significant according to duration of epileptic onset. There was significant difference in toxoplasma antibody prevalence by location, precentage of positive reaction showed 89.3% in rural compared to 69.6% in urban epileptics. This results suggested that epilepsy might be partially caused by toxoplasmosis.

개구리 식도의 섬모운동에 미치는 Morphine의 영향

은홍배,조규박 전북대학교 의과학연구소 1983 全北醫大論文集 Vol.7 No.1

The effect of morphine on the ciliary movement was studied in the frog esophagus to examine whether opiate receptor is present in the extra-neuronal site and whether change of electrolytes influences to the action of morphine on the ciliary movement. The activity of ciliary movement was measured by Hills method and the modified Krebs-Henseleit bicarbonate buffer solution was used in this study. Morphine elicited an dose-dependant delay of the ciliary movement. This inbibitory action of morphine was weakened or abolished in the presence of eserine, atropine or in the hemicholinium-treated preparation. In the atropine or hemicholinium-treated preparation, the speed of tobacco seed was rather increased. Increase of Ca^++ in the Ringer solution decreased the inhibitory action of morphine, while increased of Mg^++ potentiated the effect of morphine. From the above results, it is suggested that the inhibitory action of morphine on the ciliary movement is partly due to decreased release of acetylcholine mediated by opiate receptor drug interaction and that extra-and/or itnra-cellular concentration of electrolytes plays an important role of the effect of morphine.

電氣衝擊이 抗體形成에 미치는 影響

殷洪培 전북대학교 의과학연구소 1980 全北醫大論文集 Vol.4 No.1

This study was undertaken to evaluate the effects of the electro-convulsion on the immune response of rabbits to sheep red blood cells(SRBC). The experimental group of rabbits were treated with electro-convulsion for 7 days and immunized with SRBC. The control groups were only immunized without electro-convulsion at the same time with experimental group. Twenty-five days after primary immunization, rabbits were secondarily immunized for determining the antibody formation to SRBC. However, for the delayed-type hypersensitivity, rabbits were challenged 32 days after primary immunization. Delayed-type hypersensitivity was assayed by measuring the diameter of induration at 24, 48, and 72hrs after challenge, and antibody titers of the sera obtained from rabbits by various interval s were determined by quantitative microtitration method. Generally, both antibody formation and delayed-type hypersensitivity to SRBC were potentiated in the experimental group than the control group. Not only hemagglutinin but also hemolysin were increased in the experimental group before secondary immunization but after secondary immunization, only the hemolysin titers were slightly increased in the experimental group. The induration as well as the intensity of delayed-type hypersensitivity to SRBC were increased in experimental group compared to control group.

側腦室內 parathion의 家兎 腎臟機能에 미치는 影響

殷洪培,曺圭朴 전북대학교 의과학연구소 1979 全北醫大論文集 Vol.3 No.-

In this study the action of parathion give directly into the lateral ventricle on the renal function of the rabbit were investigated. 1. Intraventricular parathion in a dose of 100㎍ elicted diuresis following antidiuresis concomitant with changes of renal blood flow, glomerular filtration rate and electrolytes in the urine porportionally. 2. Diuretic effect of intraventricular parathion was inhibited by vagotomy, intravenous atropine or intraventriculal atropine. 3. Antidiuretic effect of intraventricular parathion was inhibited by intravenous or intraventricular regitine. From the above results, it seems most likely that renal effect induced by intraventricular parathion was brought about by renal hemodynamic changes as a result of parasympathetic and sympathetic stimulation, suggesting a possible involvement of the central cholinergic and adrenergic mechanism as well as the peripheral nerves.

電氣刺戟이 家兎腦에 對한 自家抗體에 미치는 影響

殷洪培 전북대학교 의과학연구소 1979 全北醫大論文集 Vol.3 No.-

The sera from rabbits with electro-convulsion were examined for cytotoxic antibododies aganist thymocytes of young rabbit. It was found that rabbits with electro-convulsion showed higher serum cytotoxicity to rabbit thymocytes when compared with normal rabbits.

精神分裂症患者에 있어서 兒童 胸腺細胞에 對한 抗體

殷洪培 전북대학교 의과학연구소 1978 全北醫大論文集 Vol.2 No.-

The sera from patients with schizophrenia and gealthy individuals were exzmined for cytotoxic antibodies against human child thymocyes of 3 years old. It was found that sera from schigophenics did not show higher cytotoxicity to child thymocyes than sera from normal controls.

精神病患에 있어서 胎兒胸腺細胞에 對한 抗體

殷洪培 전북대학교 의과학연구소 1977 全北醫大論文集 Vol.1 No.-

The sera from patients with mental disorders(chronic schizophrenia, paranoid psychosis, manic-depressve psychosis, psychoneurosis) and healthy individuals were examined for cyto toxic antibodies against human fetal thymocytes of 6 months in gestation. It was found that the mental disorders showed nonsignificant sytotoxicity to fetal thymocytes except one chronic schizophrenic when compared with normal controls. The cytotoxic index of one serum among 25 chronic schizophrenics was 4.

백서와 기니픽 대뇌 피질의 오피오이드 Kappa-2수용체 결합특성

조홍철,김기원,은홍배 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.2

Opioid-수용체에서는 μ,δ그리고 κ의 세가지 주된 형이 존재함이 알려져 있는바, 최근 수용체 동정기업과 선택적인 약물의 개발로 인해 그 아류들이 존재한다고 보고되고 있다. 본 연구에서 백서와 기니픽 대뇌 피질 세포막 표본에서 opioid μ와 δ-수용체를 각각의 선택적인 결합약물로 봉쇄한 후 수종의 κ촉진제와 길항제의〔³H〕diprenorphine(DIP) 결합 친화력 억제에 대한 Na+ion와 GTPΥS의 영향을 관찰하여 이를 지표로 각 ligand의 수용체에서의 작용양상을 구명코자 하였다. 각 표본에서 DIP는〔³H〕DIP 결합부위에서 서로 다른 Ki치를 보였고, U69는 백서 표본에서와 달리 기니픽 표본에서는〔³H〕DIP 결합부가 서로 다름을 보였다. 배양액내 NaC1을 NMDG로 대치시 기니픽에서는 EKC, bremazocine 그리고 U69593의 Ki치는 감소되었으며, 백서에서는 EKC의 Ki는 감소되었으나, bremazocine과 DIP와 Ki치의 변동은 없었다. 백서에서 배양액내 EKC의 친화력은 GTPΥS 50μM에 의해서는 변동되지 않았으나, 100μM에 의해서는 현저히 감소되었으며, DIP와 BRM의 친화력은 GTPΥS 50μM또는 100μM에 의해 현저히 감소되었고, DIP의 친화력은 변동되지 않았다. 본 실험성적은 guanine nucleotides와 Na+ion은 κ-수용체 길항제의 친화력에는 영향을 주지 않으나, 촉진제의 친화력을 감소시키며, opioid κ₂-수용체에 비해 κ₁-수용체는 guanine nucleotides와 Na+ion에 보다 민감하게 조절되며, bremazocine이 κ₂-수용체에 대해서는 길항제로써 작용할 수 있음을 증명하였다. It has been widely accepted that there are 3 types of opioid receptors in mammalian brain, namely μ,δand κ. Recently, multiplicity of each type of opioid receptor was established owing to advance of receptor identification technology and development of more selective ligands. Even though the presence of subtype of κ-opioid receptor was reported repeatedly, selective agonist of antagonist for κ₂-opioid receptor has not been found yet. In this study, the influences of GTPΥS, a nonhydrolyzable guanine nucleotide analogue, and Na+on binding affinity and density of several known κ-agonists and antagonists for〔³H〕dipernorphine(DIP) binding site in the presence of excess of DAMGO(1μM) and DPDPE(1μM) as blocking ligands for μ and δsites. Competition analysis in both rat and guinea pig cortex has shown a single population of〔³H〕DIP binding site with different Kd values. U69,593(U69) could not inhibit the〔³H〕DIP binding even at 10μM concentration. By the replacement of NaC1 with NMethyl-D-Glucamine(NMDG), the Ki values of DIP and Bremazocine(BRM) were not changed, and of ethylketocyclazocine(EKC) was decreased in the rat. But, in the guinea pig, the Ki values of EKC and BRM were decreased leaving the of DIP unchanged. This suggests the BRM would possess the decreased leaving the of DIP unchanged. This also suggests that BRM would possess the antagonistic property at κ₂site which is dominant type of κ-opioid receptor in the rat cortex. The Ki values of EKC were increased by the addition 100μM of GTPΥS but not by 50μM in the rat. In guinea pig cortex, the Ki values of U69, EKC and BRM were increased by both 50 and 100μM of GTPΥS. This results suggest that κ₁opioid receptor is more sensitive to Na+ and guanine nucleotide than κ₂site, and bremazocine apparently possesses antagonistic property at κsite.

實驗的不眠에 의한 白鼠 小腦 Purkinje細胞內의 PAS : 陽性顆粒數의 變動에 對하여

殷洪培 大韓神經精神醫學會 1968 신경정신의학 Vol.7 No.1

Using the cerebellum of adult rats(220-24Cgm of body weight), PAS-positive granules such as ceroids and lipofuchsin in Purkinje cells was studied histochemically and statistically. 1. PAS-positive granules present in the rat cerebellum was resistant to saliva digestion and showed slight metachromatic reaction to the 0.5% toluidine blue aqueous solution. And no significant changes in size and number of the granules was observed, after pyridine extraction. These histochemical results may indicate that PAS-positive granules in Purkinje cells are composed mainly of carbohydrate-protein complex, mucopolysaccharide or mucoprotein. 2. Number of PAS-positive granules in a Purkinje cell of the experimental groups, which had sleeping disturbance by external stimulation for 7 days, are compared with those of control groups. Average number of PAS-positive granules in the cell of experimental groups was 6.7±0.4, whereas that of the control groups was 8.5±0.4. Difference of mean value calculated was statistically significant(p<0.01). These results were discussed in relation to the formation and accumulation of the granules in the cells during the experimental condition.

알코올 투여가 백서 면역반응 및 종양 발생에 미치는 영향

안영진,하대유,은홍배 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.1

본 실험은 알코올 장기투여와 알코올 투여중단이 면역 반응 잠재력, 3-Methylcholanthrene(MCA)로 유도한 종양발생에 있어서 성차별의 유무, 종양발생 빈도, 종양을 가지는 백서(TBM)당 종양의 수를 알아보고자 실시하였다. 또한 본 실험은 알코올 투여군와 알코올 중단군의 IL-2 생산과 NK세포 활성(NKCA)을 알아보고자 실시하였으며 다음과 같은 결과를 보였다. 1) 관찰한 기간에 있어서 알코올 비투여군과 알코올 투여군간의 종양발생 빈도에는 유의한 차이가 없었으나 TBM당 종양의 수는 알코올 투여군이 알코올 비투여군에 비하여 MCA 도포후 95일째에 유의하게 많았으며 알코올 투여중단군의 종양수가 알코올 계속투여군에 비하여 유의하게 적었다. 2) 종양 발생율의 성별차이는 모든 군에서 MCA 도포후 65일째에 웅성 백서에서 높았으나 45일째, 80일째 및 95일째에는 웅성과 자성 사이에 유의한 차이는 없었다. 또한 TBM당 종양발생 수도 모든 군에서 MCA 도포후 65일째, 80일째 및 95일째에 웅성에서 많았다. 3) 비장세포의 IL-2 생산은 알코올 비투여군에 비하여 알코올 투여군에서 현저히 감소되었다. 그러나 알코올 계속투여군과 알코올 투여중단군 사이에 IL-2 생산 차이는 없었다. 4) 비장세포의 NK 활성은 알코올 비투여군에 비하여 알코올 투여군에서 유의하게 낮았으나, 알코올 계속투여군과 알코올 투여중단군간에는 NK 활성에 있어서 MCA 투여후 65일째를 제외하고는 유의한 차이가 관찰되지 않았다. 결론적으로, 알코올 투여가 IL-2와 NKCA를 억제시키나 종양증식은 증가시킴을 알 수 있었으며 알코올 투여중단은 TBM당 종양의 수를 감소시키나 이전의 알코올 투여로 인하여 억제된 IL-2 생산 억제와 NKCA억제를 역전시키지는 못하였다. For the investigation of the effect of long-term continuous ethanol(EOH) administration and EOH withdrawal on the immune response potential and sexual dimorphism in the tumorigenesis induced by 3-Methycholanthrene(MCA), the frequency of tumor production and the number of tumors per tumor bearing mouse(TBM) in female and male mice were examined. The production of IL-2 and natural killer cell activity(NKCA) of murine splenocyte were also examined. It was found that EOH feeding may suppress IL-2 production and NKCA but may enhance tumor growth, and there was a sexual dimorphism in tumor production in mice. It was also found that EOH withdrawal decreased the number of tumors per TBM, and EOH termination did not reverse the depression of both IL-2 production and NKCA caused by the previous EOH feeding.