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Using the cerebellum of adult rats(220-24Cgm of body weight), PAS-positive granules such as ceroids and lipofuchsin in Purkinje cells was studied histochemically and statistically. 1. PAS-positive granules present in the rat cerebellum was resistant to saliva digestion and showed slight metachromatic reaction to the 0.5% toluidine blue aqueous solution. And no significant changes in size and number of the granules was observed, after pyridine extraction. These histochemical results may indicate that PAS-positive granules in Purkinje cells are composed mainly of carbohydrate-protein complex, mucopolysaccharide or mucoprotein. 2. Number of PAS-positive granules in a Purkinje cell of the experimental groups, which had sleeping disturbance by external stimulation for 7 days, are compared with those of control groups. Average number of PAS-positive granules in the cell of experimental groups was 6.7±0.4, whereas that of the control groups was 8.5±0.4. Difference of mean value calculated was statistically significant(p<0.01). These results were discussed in relation to the formation and accumulation of the granules in the cells during the experimental condition.
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Using the cerebellum of adult rats(220- 24Cgmof bcdy weight), PAS- positive granules such as ceroids and lipofuchsin in Purkinje cells was studied histochemically and statistically. 1. PAS - positive granules present in the rat cerebellum was resistant to saliva digestion, and showed slight metachromatic reaction to the 0.5% toluidine blue aqueous solution. And no significant changes in size and number of the granules was observed, after pyridine extraction. These histochemical results may indicate that PAS- positive granules in Purkinje cells composed mainly of carbohydrate- protein plex, mucopolysaccharide or mucoprotein. 2. Number of PAS- positive granules in a Purkinje cell of the experimental groups, which had sleeping disturbance by external sti mulation for 7 days, are compared with those of control groups. Average number of PAS- positive granules ia the cell of experimental groups was 6.7 ± 0.4 , whereas that of the control groups was 8.5 ± 0 . 4. Difference of mean value calculated was statistically significant (p〈0 . 01). These results were discussed in relation to the formation and accumulation of the granules in the cells during the experimental condition.
The effect of morphine on the ciliary movement was studied in the frog esophagus to examine whether opiate receptor is present in the extra-neuronal site and whether change of electrolytes influences to the action of morphine on the ciliary movement. The activity of ciliary movement was measured by Hills method and the modified Krebs-Henseleit bicarbonate buffer solution was used in this study. Morphine elicited an dose-dependant delay of the ciliary movement. This inbibitory action of morphine was weakened or abolished in the presence of eserine, atropine or in the hemicholinium-treated preparation. In the atropine or hemicholinium-treated preparation, the speed of tobacco seed was rather increased. Increase of Ca^++ in the Ringer solution decreased the inhibitory action of morphine, while increased of Mg^++ potentiated the effect of morphine. From the above results, it is suggested that the inhibitory action of morphine on the ciliary movement is partly due to decreased release of acetylcholine mediated by opiate receptor drug interaction and that extra-and/or itnra-cellular concentration of electrolytes plays an important role of the effect of morphine.
This study was undertaken to evaluate toxoplasma antibody prevalence by Sabin-Feldman dye-test in epileptics(n=51), schizophrenica(n=24), and normal persons(n=60). Fourfold serum dilutions were used, we considered tilters of 1:16 or more positive. The number and percentage of positive reaction in dye-test was shown as follows : 42(82.3%) of 51 epileptics, 13 (54.1%) of 60 normal persons. Toxoplasma antibody prevalence in epileptics was not significant according to duration of epileptic onset. There was significant difference in toxoplasma antibody prevalence by location, precentage of positive reaction showed 89.3% in rural compared to 69.6% in urban epileptics. This results suggested that epilepsy might be partially caused by toxoplasmosis.
This study was undertaken to evaluate the effects of the electro-convulsion on the immune response of rabbits to sheep red blood cells(SRBC). The experimental group of rabbits were treated with electro-convulsion for 7 days and immunized with SRBC. The control groups were only immunized without electro-convulsion at the same time with experimental group. Twenty-five days after primary immunization, rabbits were secondarily immunized for determining the antibody formation to SRBC. However, for the delayed-type hypersensitivity, rabbits were challenged 32 days after primary immunization. Delayed-type hypersensitivity was assayed by measuring the diameter of induration at 24, 48, and 72hrs after challenge, and antibody titers of the sera obtained from rabbits by various interval s were determined by quantitative microtitration method. Generally, both antibody formation and delayed-type hypersensitivity to SRBC were potentiated in the experimental group than the control group. Not only hemagglutinin but also hemolysin were increased in the experimental group before secondary immunization but after secondary immunization, only the hemolysin titers were slightly increased in the experimental group. The induration as well as the intensity of delayed-type hypersensitivity to SRBC were increased in experimental group compared to control group.
In this study the action of parathion give directly into the lateral ventricle on the renal function of the rabbit were investigated. 1. Intraventricular parathion in a dose of 100㎍ elicted diuresis following antidiuresis concomitant with changes of renal blood flow, glomerular filtration rate and electrolytes in the urine porportionally. 2. Diuretic effect of intraventricular parathion was inhibited by vagotomy, intravenous atropine or intraventriculal atropine. 3. Antidiuretic effect of intraventricular parathion was inhibited by intravenous or intraventricular regitine. From the above results, it seems most likely that renal effect induced by intraventricular parathion was brought about by renal hemodynamic changes as a result of parasympathetic and sympathetic stimulation, suggesting a possible involvement of the central cholinergic and adrenergic mechanism as well as the peripheral nerves.
The sera from rabbits with electro-convulsion were examined for cytotoxic antibododies aganist thymocytes of young rabbit. It was found that rabbits with electro-convulsion showed higher serum cytotoxicity to rabbit thymocytes when compared with normal rabbits.