Repression of Transcriptional Activity of Estrogen Receptor alpha by a Cullin3/SPOP Ubiquitin E3 Ligase Complex

변부형,Yunhwa Jung 한국분자세포생물학회 2008 Molecules and cells Vol.25 No.2

The role of SPOP in the ubiquitination of ERα by the Cullin3-based E3 ubiquitin ligase complex was investigated. We showed that the N-terminal region of SPOP containing the MATH domain interacts with the AF-2 domain of ERα in cultured human embryonic 293 cells. SPOP was required for coimmunoprecipitation of ERα with Cullin3. This is the first report of the essential role of SPOP in ERα ubiquitination by the Cullin3- based E3 ubiquitin ligase complex. We also demonstrated repression of the transactiva

인삼추출물이 포화지방산 Lard 와 Alcohol 을 먹인 흰쥐 혈청 지질성분에 미치는 영향

변부형 한국식품위생안전성학회 1999 한국식품위생안전성학회지 Vol.14 No.2

The present study has been undertaken to investigate the effects of crude ginseng extract added to food on the lipid composition in serum of rats fed lard and alcohol. Thirty-five males of Sprague-Dawley strains weighed about 130 g were divided into 7 groups, each group receiving a different diet for 10 weeks ; i.e. basal diet plus 15% lard, basal diet plus 5% alcohol, basal diet plus 0.5% crude ginseng extract, basal diet plus 15% lard and 0.5% crude ginseng extract. Determinations were carried out on the net weight gain, food efficiency ratio, weight of organs, triglyceride, total cholesterol, HDL-cholesterol, free cholesterol. The results obtained were as follows: 1. Rats given feed containing lard and alcohol showed significant decrease in net weight gain, but crude ginseng extract caused an increase in food efficiency ratio. 2. Lard supplementation group showed increase in the weight of liver, kidney, spleen, but another groups did not. 3. The contents ratio of triglyceride, total cholesterol in serum of lard-fed group showed significant increase compared to controls and but the simultaneous supplementation of crude ginseng extract and lard decreased sinificantly than those of lard-fed group. 4. The contents of free-cholesterol and ester cholesterol in serum of the simultaneous supplementation of crude ginseng extract group decreased as compared with the lard single fed group. 5. Crude ginseng extract single-fed group showed increse of plasma HDL-cholesterol compared to the control but not in any other groups. 6. The ratio of contents of VLDL, LDL-cholesterol and HDL-cholesterol in serum was significantly high in the only lard containing group and alcohol group, the crude ginseng extract supplemented group was showed lower tendency than only lard and alcohol group. The above results suggest that crude ginseng extract would prevent the metabolic disease by improving hyperlipidemia.

Lard와 Alcohol을 攝取한 흰쥐血淸中의 脂質成分에 마늘이 미치는 影響

변부형 慶山大學校 1995 論文集 Vol.13 No.1

The present study has been undertaken to investigate the effects of garlic added to food on the lipid composition in serum of rats fed lard and alcohol. Thirty-five males of Sprague-Dawley strains weighed about 130g were divided into 7 groups, each group receiving a different diet for 10 weeks ; i.e. basal diet plus 15% lard, basal diet plus 5% alcohol, basal diet plus 0.5% garlic, basal diet plus 15% lard and 0.5% garlic. Determinations were carried out on the net weight gain, food efficiency ratio, weight of organs, triglyceride, total cholesterol, HDL-cholesterol, free cholesterol. The results obtained were as follows : 1. Rats given feed containing lard and alcohol showed significant decrease in net weight gain, but garlic caused an increase in food efficiency ratio. 2. Lard supplementation caused an increase in the weight of liver, kidney, spleen, but another groups did not. 3. The contents ratio of triglyceride, total cholesterol in serum of lard-fed group showed significantly increase compared to controls and but the simultaneous Supple-mentation of garlic and lard decreased significantly than those of lard-fed group. 4. The contents of free-cholesterol and ester cholesterol in serum of the simultaneous supplementation of garlic decreased as compared with the lard single fed group. 5. Garlic single-fed group increased plasma HDL-cholesterol compared to controls but in any other groups were not changed. 6. The ratio of contents of VLDL, LDL-cholesterol and HDL-cholesterol in serum was significantly high in the only lard containing group and alcohol group, the garlic supplemented group was showed lower tendency than only lard and alcohol group. The above results suggest that garlic would prevent the metabolic disease by improv-ing hyperlipemia.

좌골신경통의 침구치료

변부형,임사비나,이승우 대구한의대학교 제한동의학술원 1999 제한동의학술원논문집 Vol.4 No.1

마늘成分이 Lard와 Alcohol을 攝取한 흰쥐 血淸中의 酵素活性에 미치는 影響

변부형 慶山大學校 1994 論文集 Vol.12 No.1

The present study has been undertaken to investigate the effects of garlic added to food on the activities of several enzymes in serum of rats lard and alcohol. Thirty-five males of Sprague-Dawley stains weighed about 130 g were divided into 7groups, earth group receiving a different diet for 10 weeks ; i.e. basal diet plus 15% lard, basal diet plus 5% alcohol, basal diet plus 0.5 % garlic, basal diet plus 15% lard and 0.5% garlic. Determinations were carried out on the net weight gain, food efficiency ratio, weight of organs, and glutamic oxa1acetic transaminase, glutamic pyruvic transaminase, lactate dehydrogenase, alkaline phogphatase activities in serum of rats. The results obtained were as follows : 1. Rats given feed containing lard and alcohol showed significant decrease in net weight gain, but garlic caused an increase in food efficiency ratio. 2. Lard supplementation caused an increase in the weight of liver, kidney, spleen, but another groups did not. 3. AST, ALT, ALP, LDH of serum were significantly increased in lard and alcohol containing group but garlic feeding decreased enzyme activities compared to lard and alcohol containing group.

마늘성분이 Lard 와 Alcohol 을 섭취한 흰쥐 혈청중의 효소활성에 미치는 영향

변부형,김석환,정혜광,남철현,노병의 한국식품위생안전성학회 1995 한국식품위생안전성학회지 Vol.10 No.3

The present study has been undertaken to investigate the effects of garlic added to food on the activities of several enzymes in serum of rats fed lard and alcohol. Thirty-five males of Sprague-Dawley strains weighed about 130g were divided into 7 groups, each group receiving a different diet for 10 weeks; i.e. basal diet plus 15% lard, basal diet plus 5010 alcohol, basal diet plus 0.5% garlic, basal diet plus 15% lard and 0.5% garlic. Determinations were carried out on the net weight gain, food efficiency ratio, weight of organs, and AST, ALT, lactate dehydrogenase, alkaline phoaphatase activities in serum of rata. The results obtained were as follows: Rats given feed containing lard and alcohol showed significant decrease in net weight gain, but garlic caused an increase in food efficiency ratio. Lard supplementation caused an increase in the weight of liver, kidney, spleen, but another groups did not. AST, ALT, ALP, LDH of serum were significantly increased in lard and alcohol containing group but garlic feeding decreased enzyme activities compared to lard and alcohol containing group. The above results suggest that garlic would prevent the metabolic disease of liver by improving hyperlipemia caused by high fat diet.

Stearic acid, Oleic acid, Elaidic acid에 의한 간세포의 성장 및 지방산 조성의 변화

변부형 慶山大學校 1997 論文集 Vol.15 No.1

This study was carried out in order to investigate the effect of stearic acid (18:0,SA), oleic (18:1cis.0A) and elaidic acid (18:1 trans, EA) on the cell growth and fatty acid composition of Hep-G2 cells. The cells were incubated in serum-free medium 25, 50, 100 and 200uM of fatty acid combined with albumin for 2 days. The fatty acid concentration up to lOOuM showed the normal growth, but the cell growth decreased in the presence of 200uM fatty acid. The treatment of cells with lOOuM of a fatty acid for two days significantly (p<0.05) increased the cellular triglyceride (TG) content in all fatty acid groups compared to control, but TG content was not significantly different among all treatment group, but total cholesterol (TC) was the highest level in EA group. Cellular phospholipid(PL) contents were similar between the control and all faty acid groups.

황금약침의 급성독성에 관한 안전성 연구

변부형 ( Byeon Bu Hyeong ),서부일 ( Seo Bu Il ) 대한본초학회 2003 大韓本草學會誌 Vol.18 No.4

연구 목적: Sprague-Daewey rat에 의한 黃芩 藥針의 安全性을 검토하기 위해 國立 保健 硏究院 急性 毒性試驗 基準을 기본으로 하여 急性 毒性 試驗을 修行하였다. 방법: 食品醫藥品 安全性 고시 제1998-116호의 `醫藥品 등의 毒性 試驗 기준`에 준하여 실시하였다. SD계 rat는 독성 시험에 적당한 실험 동물로서 急性 毒性 試驗에 널리 사용되고 있다. 본 계통의 rat는 풍부한 試驗基礎資料가 蓄積되어 있어서, 評價 時 實驗 結果의 해석이 용이하다. 결과: 對照群을 포함한 모든 實驗群에서 外觀 및 一般 行動에서의 이상 症狀은 觀察되지 않았으며, 實驗군에 藥針을 시술한 직후의 行動 이상 및 投與 직후 사이의 行動 이상도 觀察되지 않았다. 또한 剖檢時 이상 소견이 對照群과 모든 投與群에서 觀察되지 않았다. 본 시험에서의 黃金藥針은 安全性에는 문제가 없는 것으로 사료되었다. 황금 약침 施術群에 대한 血液 生化學적인 檢査에서 glucose의 농도는 증가하는 경향(P<0.05)을 나타냈다. 반면에 GOT, GPT 농도는 黃金 藥針 施術群에서 오히려 감소하는 有意性 있는 흥미로운 結果를 얻을 수 있었다. 결론: 食品醫藥品 安全性 고시 제1998-116호의 `醫藥品 등의 毒性 試驗 기준`에 준하여 黃金藥針의 急性 毒性 試驗을 修行한 結果 본 試驗에서의 黃金 藥針은 安全性에는 문제가 없는 것으로 사료되었다. N/A

길경 분말이 고지방식이로 유도된 비만 흰쥐 혈청 중의 간효소 활성도에 미치는 영향

변부형 ( Byeon Bu Hyeong ),서부일 ( Seo Bu Il ) 대한본초학회 2003 大韓本草學會誌 Vol.18 No.4

N/A Objectives: The present study has been undertaken to investigate the effects of Platycodi Radix powder on the activities of several enzymes in serum of rats fed high fat diet. Methods: Twenty four males of Sprague-Dawley strains weighed about 580g were divided into 4 groups, each group receiving a different diet for 10 weeks ; i.e. control group, control+ 20% Platycodi Radix powder group, high fat diet group, high fat diet + 20% Platycodi Radix powder group. Determinations were carried out on the net weight gain, food efficiency ratio, and AST, ALT, lactate dehydrogenase, alkaline phosphatase activities in serum of rats. Results: The results obtained were as follows : AST, ALT, ALP, LDH of serum were significantly increased in lard containing group but Platycodi Radix powder feeding groups decreased enzyme activities compared to lard group. Conclusions: The above results suggest that Platycodi Radix powder would prevent the metabolic disease of liver by preventing hyperlipemia caused by high fat diet.

V79 배양 세포에 있어 인삼추출물이 DNA복제 및 회복 합성에 미치는 분자생물학적 연구

卞富亨,徐富一,卞晟僖 대한본초학회 1999 大韓本草學會誌 Vol.14 No.2

본 연구는 인삼추출물이 DNA 복제 및 회복 합성에 미치는 영향을 조사하기 위해 수행되었다. DNA 복제 및 회복 합성도의 측정은 V79 chinese hamster lung cell의 핵 내 DNA에 [3H]-tymidine이 유입되는 양으로 측정하였다. 그 결과 인삼추출물의 농도(0~1ug/㎖)가 높을수록 점점 더 크게 감소하였다. 이와 같이 인삼추출물에 의하여 DNA 복제 합성도가 감소하는 현상은 농도가 더 높을수록 효과적인 것으로 보아 농도에 의존한다는 것을 알 수 있었다. 한편 DNA 회복 합성도에 인삼추출물이 미치는 영향을 알아보기 위해 우선 DNA를 손상시켰다. DNA를 손상시키는 물질은 여러 가지 있으나, 본 실험에서는 자외선과 methylmethane sulfonate(MMS)를 사용하였다. 이렇게 손상된 DNA가 인삼추출물(0~1ug/㎖)에 의하여 얼마나 회복되었는가를 알아보기 위하여 인삼추출물의 농도를 각각 달리하여 V79 cell에 첨가하였다. 그 결과 인삼추출물의 농도가 높을수록 DNA 회복합성도는 증가하였다. 이와 같이 인삼추출물이 DNA 복제 합성은 억제하고, DNA 회복 합성은 증가시키는 상반된 효과를 보여 주었다. 이러한 상반된 효과는 복제합성과 회복합성의 두 과정에서 서로 다른 효소체계가 관여하기 때문인 것으로 보인다. 인삼추출물이 DNA복제 및 회복합성에 미치는 영향에 대하여 보다 더 구체적인 분자생물학적 메커니즘 연구가 이루어져야 할 것이다.