Indoor feeding combined with restricted grazing time improves body health, slaughter performance, and meat quality in Huang-huai sheep

Huang Yafeng,Zhao Mengyu,Zhang Xiaoan,Wei Huiqing,Liu Lumeng,Zhang Zi Jun,Cheng Xiao,Wang Guanjun,Ren Chunhuan 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.11

Objective: The aim of this study was to evaluate the effects of three feeding systems, i.e., indoor feeding (CON), indoor feeding with 4-h daily access to grazing artificial pasture (ITGP), and indoor feeding with 8-h daily access to grazing artificial pasture (IEGP), on the plasma antioxidant and immunological capacity, slaughter characteristics, meat quality and economic efficiency of Huang-huai lambs. Methods: Thirty-three healthy Huang-huai rams with similar body weight (approximately 5 mo of age, 28.96±1.01 kg) were assigned equally to three experimental groups. When finished fattening, six lambs from each group were collect blood samples for plasma analyses and then slaughtered to determine slaughter characteristics and obtain biceps brachii muscle for further analysis of meat quality and fatty acid profile. Results: Compared to CON group, animals submitted to ITGP and IEGP groups resulted in greater contents of serum glutathione peroxidase, immunoglobulins (IgA, IgG, and IgM), polyunsaturated fatty acids (PUFA), n-6 PUFA, and PUFA/saturated fatty acid (FA) ratio and lower palmitic /oleic acid ratio (p<0.05). Moreover, animals in ITGP group exhibited a higher (p<0.05) loin eye area, content of meat crude protein (CP), and eicosetrienoic acid compared to CON group, while slaughter performance was superior (p<0.05) to that of the IEGP group. The economic efficiency of ITGP group was 70.12% higher than that of CON group, while the IEGP group exhibited a decrease of 92.54% in economic efficiency compared to the CON group. Conclusion: Restricted grazing time combined with indoor feeding was more effective in conferring superior body health, carcass traits and economic efficiency in Huang-huai lambs, as well as higher CP content and healthier FA composition in the resulting meat.

A Clinical Study on Juheli (Recombinant Human Interleukin - 11) in the Second Prevention of Chemotherapy Induced Thrombocytopenia

Xiao, Yang,Liu, Jun,Huang, Xin-En,Guo, Jian-Xiong,Fu, Peng-Chao,Huang, Xiao-Hong,Zhou, Juan,Ye, Ai-Qin Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.2

Objective: to investigate the effect and side effects of recombinant human interleukin - 11 (rhIL - 11, in Chinese Juheli, produced by Qi Lu Biotechnology CO., LTD) in the second prevention of chemotherapy induced thrombocytopenia (CIT). Methods: Cancer patients with CIT were recruited and were treated with rhIL - 11 (treatment phase, TP), and in the following cycle, all these patients administered with rhIL - 11 24 hours immediately after chemotherapy (preventive treatment phase, PTP). Duration and severity of thrombocytopenia between two phases were compared. Results: for patients in TP or PTP, nadir values of platelet were ($29.28{\pm}20.08){\times}10^9/L$ and ($45.24{\pm}19.66){\times}10^9/L$, duration of thrombocytopenia in TP and PTP was ($11.52{\pm}4.33$) and ($8.20{\pm}+2.77$)days, recovery time was ($19.40{\pm}3.89$)and ($13.44{\pm}3.02$)days, duration of rhIL - 11 administration was ($10.68{\pm}2.46$)and ($6.28{\pm}1.77$)days, number of patients needing platelet infusion was 16and4 respectively, all differences were statistically significant (p value were 0.007, 0.002, 0.000, 0.000, 0.034 respectively). For TP and PTP, number of patients with hemorrhage was 8 and 4, duration of bleeding was ($5.00{\pm}0.82$) and ($4.50{\pm}0.71$) days respectively, with no statistically significant difference. Adverse reactions mainly included fever, edema, arrhythmia, joint pain, fatigue, skin rash, headache, dizziness, etc., all were not statistically significant between TP and PTP. Conclusion: rhIL - 11 could be well tolerated and is effective that could reduce the duration, severity of CIT, platelet transfusion, and incidence of bleeding, as well as shorten the recovery time, duration of rhIL - 11 administration. Thus, rhIL - 11 could be commended in the second prevention of CIT for patients with cancer.

Clinical Study on Fluvoxamine Combined with Oxycodone Prolonged-Release Tablets in Treating Patients with Moderate to Severe Cancer Pain

Xiao, Yang,Liu, Jun,Huang, Xin-En,Ca, Li-Hua,Ma, Yi-Min,Wei, Wei,Zhang, Rong-Xia,Huang, Xiao-Hong,Chang, Juan,Wu, Yi-Jia Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Objective: To observe treatment effects and safety of fluvoxamine combined with oxycodone prolonged-release tablets in treating patients with moderate to severe cancer pain. Methods: Patients confirmed pathologically with cancer and complicated with moderate to severe pain, were divided into control and experimental groups. Oxycodone prolonged-release tablets, with or without fluvoxamine, were administrated to all study patients until pain relief. Degree of pain relief, dose of oxycodone prolonged-release tablets, side effects and quality of life were compared before and after treatment. Results: In total, 120 patients were recruited. No statistically significant difference was detected regarding age, gender, types of cancer, KPS between two groups of patients (P>0.05). Baseline pain score of patients with moderate pain in treatment and control group was $4.9{\pm}0.8$ and $5.1{\pm}0.8$, respectively; and decreased to $1.8{\pm}1.1$ and $1.2{\pm}1.1$ after treatment, respectively. Pain intensity was significantly reduced in the treatment group (P=0.028). Average daily consumption of oxycodone prolonged-release tablets was ($54.0{\pm}19.6$) mg and ($44.7{\pm}18.7$) mg respectively, which is lower in treatment grpup than in control group, but the difference was not statistically significant (P=0.065). Baseline pain score of patients with severe pain in treatment and control groups were $8.3{\pm}1.1$ and $8.3{\pm}1.1$, respectively; and pain intensity after treatment decreased to $2.9{\pm}1.0$ and $2.3{\pm}1.0$. Pain intensity was significantly reduced in the treatment group, with statistical significance (P=0.026). Average daily consumption of oxycodone prolonged-release tablets was ($132.0{\pm}42.2$) mg and ($110.7{\pm}33.9$) mg, respectively, which is lower in treatment group than in control group, and the difference was statistically significant (P=0.035). In terms of quality of life, patients in treatment group had better performance status, daily activity, mood, and sleep than that in control group (P < 0.05). Patients in two groups had similar side effects, eg., constipation, nausea/vomiting, lethargy, dizziness, itchy skin, dysuria, and ataxia. Lower incidence of nausea/vomiting, lethargy, was obtained from patients in treatment than in control group, while significant low constipation was observed in treatment than in control group (35.0% vs 49.2%, P=0.026). Conclusion: Fluvoxamine combined with oxycodone prolonged-release tablets could be more effective in treating patients with cancer pain, and could reduce the dosage of oxycodone prolonged-release tablets and thus be associated with lower side effects, and improved quality of life.

Molecular identification and the role of adipokinetic hormone receptor in Helicoverpa armigera (Hübner)

Xiao Zhengkun,Jin Ruoheng,Yun Tan,Tang Jun,Huang Guo-Hua 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

In insects, lipids and trehalose are the primary energy reserves and sources, and their metabolism is controlled by a variety of physiological processes. Adipokinetic hormone receptor (AKHR) has been reported to be involved in glycolipid metabolism and immune response, however, is an uncharted territory in Helicoverpa armigera. In the present study, the full-length cDNA encoding AKHR was isolated from H. armigera. The HaAKHR contains a seven-transmembrane domain, which was a characteristic of G-protein coupled receptors. The expression profile analysis revealed that HaAKHR was highly expressed in fat body of newly emerged female adults and highly expressed in the immature stage. The content of triglyceride (TAG) in fat body increased significantly after silencing of HaAKHR, however, the trehalase activity and the expression of trehalose-6-phosphate synthase (TPS) were decreased in hemolymph. Furthermore, the expression level of HaAKHR was significantly upregulated when infected with different kinds of entomopathogenic microbes. The above results suggested that HaAKHR plays an important role in regulating glycolipid metabolism and immune response in H. armigera, and contribute to understanding the underlying mechanism of host-pathogen interactions in H. armigera.

Performance optimization of flexible a-Si:H solar cells with nanotextured plasmonic substrate by tuning the thickness of oxide spacer layer

Xiao, Huapeng,Wang, Jun,Huang, Hongtao,Lu, Linfeng,Lin, Qingfeng,Fan, Zhiyong,Chen, Xiaoyuan,Jeong, Chaehwan,Zhu, Xufei,Li, Dongdong Elsevier 2015 Nano energy Vol.11 No.-

<P><B>Abstract</B></P> <P>Plasmonic thin film solar cells deposited on periodically textured photonic crystal substrates have been extensively studied since the substantially enhanced light absorption. The reduction of parasitic absorption losses in the metal and spacer layers becomes one of the key issues to achieve high efficiency solar cells. Herein, plasmonic amorphous silicon (a-Si:H) flexible thin film solar cells with different thickness of oxide spacer layers are systematically investigated. An increase of the spacer layer thickness leads to an evolution in surface morphology of AZO and final devices. More intriguingly, the increase of spacer layer thickness reduces the absorption in Ag layer while induces more absorption in spacer layer. The highest light absorption in silicon layer is observed as applying 100nm spacer layer, which is further verified by electrical measurements. Our observations demonstrate a versatile and convenient route towards rational design of light harvesting nanostructure for high performance plasmonic solar cells based on a broad range of materials.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Amorphous silicon thin film solar cells are constructed on patterned substrates. </LI> <LI> The devices properties are studied as a function of spacer layer thickness. </LI> <LI> An increase of spacer layer thickness reduces the absorption loss of Ag layer. </LI> <LI> The device with 100nm spacer layer confines more incident light in silicon layer. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

A New Triterpenoid from Panax ginseng Exhibits Cytotoxicity through p53 and the Caspase Signaling Pathway in the HepG2 Cell Line

Huang, Jian,Tang, Xiao-Hui,Ikejima, Takashi,Sun, Xiu-Jia,Wang, Xiao-Bo,Xi, Rong-Gang,Wu, Li-Jun 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.3

A new triterpenoid, 20(R),22$({\zeta})$,24(S)-dammar-25(26)-ene-$3{\beta},6{\alpha},12{\beta}$,20,22,24-hexanol (1), and three known triterpenoids, ${\beta}$-D-glucopyranoside,($3{\beta},12{\beta}$)-12,20-dihydroxydammar-24-en-3-yl,6-acetate (2), 20(R)-ginsenoside $Rg_3$ (3), and 20(R)-ginsenoside $Rg_2$ (4), were isolated from the leaves of Panax ginseng. Their structures were determined by chemical analysis and spectral methods (IR, 1D and 2D NMR, HR-ESI-MS). Compounds 1-4 were exhibited various degrees of cytotoxicity in the human hepatoma cell line, HepG2. Compound 1 had the highest cytotoxic Potency, with an $IC_{50}$ value of 20.1 ${\mu}M$, by stimulating p53-mediated cell cycle arrest at the G1 to S phase transition, leading to apoptosis via activation of the caspase signaling pathway.

Flexural behavior of ultra high performance concrete beams reinforced with high strength steel

Jun-Yan Wang,Jin-Ben Gu,Chao Liu,Yu-Hao Huang,Ru-Cheng Xiao,Biao Ma 국제구조공학회 2022 Structural Engineering and Mechanics, An Int'l Jou Vol.81 No.5

A detailed experimental program was conducted to investigate the flexural behavior of ultra high performance concrete (UHPC) beams reinforced with high strength steel (HSS) rebars with a specified yield strength of 600 MPa via direct tensile test and monotonic four-point bending test. First, two sets of direct tensile test specimens, with the same reinforcement ratio but different yield strength of reinforcement, were fabricated and tested. Subsequently, six simply supported beams, including two plain UHPC beams and four reinforced UHPC beams, were prepared and tested under four-point bending load. The results showed that the balanced-reinforced UHPC beams reinforced with HSS rebars could improve the ultimate loadbearing capacity, deformation capacity, ductility properties, etc. more effectively owing to interaction between high strength of HSS rebar and strain-hardening characteristic of UHPC. In addition, the UHPC with steel rebars kept strain compatibility prior to the yielding of the steel rebar, further satisfied the plane-section assumption. Most importantly, the crack pattern of the UHPC beam reinforced with HSS rebars was prone to transform from single main crack failure corresponding to the normal-strength steel, to multiple main cracks failure under the condition of balanced-reinforced failure, which validated by the conclusion of direct tensile tests cooperated with acoustic emission (AE) source locating technique as well.

Augmenter of Liver Regeneration Alleviates Renal Hypoxia-Reoxygenation Injury by Regulating Mitochondrial Dynamics in Renal Tubular Epithelial Cells

Xiao-hui Liao,Rui-ting Long,Jun-bo Peng,Li-li Huang,Gui-ping Jiang,Yue-juan Liao,Hang Sun,Yu-dong Hu 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.12

Mitochondria are highly dynamic organelles that constantly undergo fission and fusion processes that closely related to their function. Disruption of mitochondrial dynamics has been demonstrated in acute kidney injury (AKI), which could eventually result in cell injury and death. Previously, we reported that augmenter of liver regeneration (ALR) alleviates renal tubular epithelial cell injury. Here, we gained further insights into whether the renoprotective roles of ALR are associated with mitochondrial dynamics. Changes in mitochondrial dynamics were examined in experimental models of renal ischemia-reperfusion (IR). In a model of hypoxia-reoxygenation (HR) injury in vitro, dynamin-related protein 1 (Drp1) and mitochondrial fission process protein 1 (MTFP1), two key proteins of mitochondrial fission, were downregulated in the Lv-ALR + HR group. ALR overexpression additionally had an impact on phosphorylation of Drp1 Ser637 during AKI. The inner membrane fusion protein, Optic Atrophy 1 (OPA1), was significantly increased whereas levels of outer membrane fusion proteins Mitofusin-1 and -2 (Mfn1, Mfn2) were not affected in the Lv-ALR + HR group, compared with the control group. Furthermore, the mTOR/4E-BP1 signaling pathway was highly activated in the Lv-ALR + HR group. ALR overexpression led to suppression of HR-induced apoptosis. Our collective findings indicate that ALR gene transfection alleviates mitochondrial injury, possibly through inhibiting fission and promoting fusion of the mitochondrial inner membrane, both of which contribute to reduction of HK-2 cell apoptosis. Additionally, fission processes are potentially mediated by promoting tubular cell survival through activating the mTOR/4E-BP1 signaling pathway.

Streptomyces zhihengii sp. nov., isolated from rhizospheric soil of Psammosilene tunicoides

Huang, Mei-Juan,Fei, Jing-Jing,Salam, Nimaichand,Kim, Chang-Jin,Hozzein, Wael N.,Xiao, Min,Huang, Hai-Quan,Li, Wen-Jun Springer-Verlag 2016 Archives of microbiology Vol.198 No.8

<P>An actinomycete strain, designated YIM T102(T), was isolated from the rhizospheric soil of Psammosilene tunicoides W. C. Wu et C. Y. Wu collected from Lijiang, Yunnan Province, China. The taxonomic position of the new isolate was investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YIM T102(T) belongs to the genus Streptomyces. Strain YIM T102(T) was most closely related to Streptomyces eurocidicus NRRL B-1676(T) with a pairwise 16S rRNA gene sequence similarity of 98.9 %. However, DNA-DNA relatedness value between strain YIM T102(T) and S. eurocidicus NBRC 13491(T) was found to be 37.8 +/- 1.8 %. The menaquinone composition detected for strain YIM T102(T) was MK-9 (H-6) and MK-9 (H-8), while the major fatty acids were summed feature 4 (38.0 %), anteiso-C-15:0 (13.1 %), iso-C-16:0 (10.1 %), summed feature 3 (9.8 %) and C-16:0 (9.0 %) and iso-C-15:0 (5.2 %). The whole-cell hydrolysates contained galactose, glucose, ribose and mannose, along with ll-diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan. The DNA G+C content was 70.7 mol%. Strain YIM T102(T) also exhibited antagonistic activity against Alternaria alternata, Alternaria brassicae and Colletotrichum nicotianae Averna, based on the findings from the comparative analyses of phenotypic and genotypic characteristics; it is proposed that strain YIM T102 represents a novel species of the genus Streptomyces, for which the name Streptomyces zhihengii sp. nov. is proposed. The type strain is YIM T102(T) (=KCTC 39115(T) = DSM 42176(T) = CGMCC 4.7248(T)).</P>

Nanoparticle Realgar Powders Induce Apoptosis in U937 Cells through Caspase MAPK and Mitochondrial Pathways

Xiao-bo Wang,Hui-yuan Gao,Bai-ling Hou,Jian Huang,Rong-gang Xi,Li-jun Wu 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.5

Nanoparticle realgar powders (NRP) inhibited U937 cell growth in a time and dose-dependent manner. U937 cells treated with NRP showed typical characteristics of apoptosis including the morphological changes and DNA fragmentation. Caspase family inhibitor (z-VAD-fmk), caspase-8, -9 inhibitor (z-IETD-fmk, Ac-LEHD-CHO, respectively) and caspase-3 inhibitor (z- DEVD-fmk) partially prevented NRP -induced apoptosis. Moreover, the classical substrates of caspase-3, poly-ADP ribose polymerase (PARP) was degraded after U937 cells treatment with NRP. In addition, NRP increased the ratio of Bax/Bcl-2 protein expression. Although p38 inhibitor (SB203580) and ERK inhibitor (PD98059) failed to block cell death, JNK inhibitor (SP600125) had marked inhibitory effects on NRP -induced apoptosis. Furthermore, the phosphorylation of JNK was up-regulated, suggesting that JNK was responsible for NRP -induced apoptosis in U937 cells. These results suggested that the caspase, mitochondria and MAPK signal pathways were involved in NRP-induced U937 apoptosis.