Carbon disulfide induces mitochondria-mediated apoptosis in Sertoli-germ cells coculture

Wei Wang,Zhen Zhang,Yinsheng Guo,Yu Dong,Xiaoyu Huang1,Yijun Zhou,Guoyuan Chen 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.2

Carbon disulfide (CS2), a common organic solvent, induces a variety of adverse effects in the male reproductive system. In this study, we investigated the cytotoxicity, ultrastructural changes, and potential apoptotic induction mechanisms of CS2 in mixed cultures of Sertoli and germ cells. Sertoli and germ cells were cocultured and treated with CS2 for 24 h. Growth rates were noted, and apoptotic cells were identified by Hoechst 33258 staining. Ultrastructure changes were observed via transmission electron microscopy (TEM). Mitochondrial membrane potential and expressions of apoptosis-related factors (cytochrome c, Bax, Bcl- 2, caspase-3 and caspase-9) were examined by JC-1 staining, western blot, and real-time PCR. The results showed that CS2 treatment was associated with reduced growth rates of Sertoli-germ cells. Ultrastructure changes in Sertoli-germ cells treated with CS2 were typical of apoptosis. In addition, CS2 treatment depolarized mitochondrial membrane potential, upregulated Bax levels and downregulated Bcl-2 levels, released cytochrome c from the mitochondrial intermembrane space to the cytosol, and triggered mitochondria-mediated apoptosis. Subsequently, caspase-9 and caspase-3 were activated, resulting in Sertoli-germ cells apoptosis. The above data suggest that CS2 has adverse effect on the viability of Sertoli-germ cells and induces apoptosis through mitochondrial pathway.

siRNA Mediated Silencing of NIN1/RPN12 Binding Protein 1 Homolog Inhibits Proliferation and Growth of Breast Cancer Cells

Huang, Wei-Yi,Chen, Dong-Hui,Ning, Li,Wang, Li-Wei Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

The gene encoding the Nin one binding (NOB1) protein which plays an essential role in protein degradation has been investigated for possible tumor promoting functions. The present study was focused on NOB1 as a possible therapeutic target for breast cancer treatment. Lentivirus mediated NOB1 siRNA transfection was used to silence the NOB1 gene in two established breast cancer cell lines, MCF-7 and MDA-MB-231, successful transfection being confirmed by fluorescence imaging. NOB1 deletion caused significant decline in cell proliferation was observed in both cell lines as investigated by MTT assay. Furthermore the number and size of the colonies formed were also significantly reduced in the absence of NOB1. Moreover NOB1 gene knockdown arrested the cell cycle and inhibited cell cycle related protein expression. Collectively these results indicate that NOB1 plays an essential role in breast cancer cell proliferation and its gene expression could be a therapeutic target.

C-type natriuretic peptide attenuates renal osteodystrophy through inhibition of FGF-23/MAPK signaling

Dong Dong Zhang,Yang Fang Wu,Wei Xia Chen,Yao Xu,Si Yan Liu,Huang Huang Luo,Guang Mei Jiang,Yue Wu,Peng Hu 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Renal osteodystrophy (ROD) occurs as early as chronic kidney disease (CKD) stage 2 and seems ubiquitous in almost all pediatric patients with CKD stage 5. Fibroblast growth factor (FGF)-23, a bone-derived endocrine regulator of phosphate homeostasis, is overexpressed in CKD and disturbs osteoblast differentiation and matrix mineralization. In contrast, C-type natriuretic peptide (CNP) acts as a potent positive regulator of bone growth. In the present study, we infused CNP into uremic rats and observed whether CNP could attenuate ROD through the inhibition of FGF-23 cascades. In uremic rats, CNP administration significantly alleviated renal dysfunction, calcium phosphate metabolic disorders, hypovitaminosis D, secondary hyperparathyroidism, the decrease in bone turnover markers and retarded bone pathological progression. More importantly, within FGF-23/mitogen-activated protein kinase (MAPK) signaling, the fibroblast growth factor receptor-1, Klotho and alternative (STAT-1/phospho-STAT-1) elements were upregulated by CNP, whereas FGF-23, RAF-1/phospho-RAF-1, and downstream (ERK/phospho-ERK and P38/phospho-P38) elements were paradoxically underexpressed in bone tissue. Therefore, CNP exerts a therapeutic effect on ROD through inhibition of FGF-23/MAPK signaling at the RAF-1 level.

Expression and Underlying Roles of IGFBP-3 in Paclitaxel-Treated Gastric Cancer Sgc-7901 Cells

Huang, Gang,Dang, Zhong-Feng,Dang, Ya-Mei,Cai, Wei,Li, Yuan,Chen, Yi-Rong,Xie, Xiao-Dong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Purpose: To study the expression of insulin-like growth factor binding proteins (IGFBPs) in paclitaxel-treated gastric cancer SGC-7901 cells, and to further investigate underlying mechanisms. Materials and Methods: Real time PCR and Western blot assays were applied to detect the mRNA and protein expression of IGFBP-2, -3 and -5 after paclitaxel (10 nM) treatment of SGC-7901 cells. In addition IGFBP-3 expression was silenced by RNA interference to determine effects. Cell viability was determined by MTT assay. Cell cycling and apoptosis were assessed by flow cytometry. Results: Compared to the control group, only IGFBP-3 expression was elevated significantly after paclitaxel (10 nM) treatment (p<0.05). Paclitaxel treatment caused cell cycle arrest and apoptosis via downregulating Bcl-2 expression. However, the effect could be abrogated by IGFBP-3 silencing. Conclusions: IGFBP-3 exhibits anti-apoptotic effects on paclitaxel-treated SGC-7901 cells via elevating Bcl-2 expression.

ATCS: An Adaptive TCP Coding Scheme for Satellite IP Networks

( Wei Dong ),( Junfeng Wang ),( Minhuan Huang ),( Jian Tang ),( Hongxia Zhou ) 한국인터넷정보학회 2011 KSII Transactions on Internet and Information Syst Vol.5 No.5

In this paper we propose ATCS, a practical TCP protocol coding scheme based on network coding for satellite IP networks. The proposal is specially designed to enhance TCP performance over satellite networks. In our scheme, the source introduces a degree of redundancy and transmits a random linear combination of TCP packets. Since the redundant packets are utilized to mask packet loss over satellite links, the degree of redundancy is determined by the link error rates. Through a simple and effective method, ATCS estimates link error rates in real time and then dynamically adjusts the redundant factor. Consequently, ATCS is adaptable to a wide range of link error rates by coding TCP segments with a flexible redundancy factor. Furthermore, the scheme is compatible with traditional TCP variants. Simulation results indicate that the proposal improves TCP performance considerably.

Protocatechuic Acid, a Gut Bacterial Metabolite of Black Raspberries, Inhibits Adenoma Development and Alters Gut Microbiome Profiles in Apc Min/+ Mice

Dong Athena,Lin Chien-Wei,Echeveste Carla Elena,Huang Yi-Wen,Oshima Kiyoko,Yearsley Martha,Chen Xiao,Yu Jianhua,Wang Li-Shu 대한암예방학회 2022 Journal of cancer prevention Vol.27 No.1

Administration of black raspberries (BRBs) and their anthocyanin metabolites, including protocatechuic acid (PCA), has been demonstrated to exert chemopreventive effects against colorectal cancer through alteration of innate immune cell trafficking, modulation of metabolic and inflammatory pathways, etc. Previous research has shown that the gut microbiome is important in the effectiveness of chemoprevention of colorectal cancer. This study aimed to assess the potency of PCA versus BRB dietary administration for colorectal cancer prevention using an ApcMin/+ mouse model and determine how bacterial profiles change in response to PCA and BRBs. A control AIN-76A diet supplemented with 5% BRBs, 500 ppm PCA, or 1,000 ppm PCA was administered to Apc- Min/+ mice. Changes in incidence, polyp number, and polyp size regarding adenomas of the small intestine and colon were assessed after completion of the diet regimen. There were significant decreases in adenoma development by dietary administration of PCA and BRBs in the small intestine and the 5% BRB-supplemented diet in the colon. Pro-inflammatory bacterial profiles were replaced with anti-inflammatory bacteria in all treatments, with the greatest effects in the 5% BRB and 500 ppm PCA-supplemented diets accompanied by decreased COX-2 and prostaglandin E2 levels in colonic mucosa. We further showed that 500 ppm PCA, but not 1,000 ppm PCA, increased IFN-γ and SMAD4 levels in primary cultured human natural killer cells. These results suggest that both BRBs and a lower dose PCA can benefit colorectal cancer patients by inhibiting the growth and proliferation of adenomas and promoting a more favorable gut microbiome condition.

A Rerouting-Controlled ISL Handover Protocol For LEO Satellite Networks

( Wei Dong ),( Junfeng Wang ),( Minhuan Huang ),( Jian Tang ),( Hongxia Zhou ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.10

In this paper, a rerouting-controlled ISL (Inter-Satellite link) handover protocol for LEO satellite networks (RCIHP) is proposed. Through topological dynamics and periodic characterization of LEO satellite constellation, the protocol firstly derives the ISL related information such as the moments of ISL handovers and the intervals during which ISLs are closed and cannot be used to forward packet. The information, combined with satellite link load status, is then been utilized during packet forwarding process. The protocol makes a forwarding decision on a per packet basis and only routes packets to living and non-congested satellite links. Thus RCIHP avoids periodic rerouting that occurs in traditional routing protocols and makes it totally unnecessary. Simulation studies show that RCIHP has a good performance in terms of packet dropped possibility and end-to-end delay.

Iron metabolism protein transferrin receptor 1 involves in cervical cancer progression by affecting gene expression and alternative splicing in HeLa cells

Huang Nan,Wei Yaxun,Cheng Yi,Wang Xiaolong,Wang Qi,Chen Dong,Li Wenjing 한국유전학회 2022 Genes & Genomics Vol.44 No.6

Background: Transferrin receptor 1 (TfR1), encoded by TFRC, is a key regulator of iron homeostasis and plays important roles in many diseases, including cancers. Objective: To decipher the underlying molecular functions of TfR1 based on its influence on transcriptome profile in cancer cells. Methods: In this study, we first identified the expression pattern and prognostic influence of TFRC in cervical cancer patients from TCGA database. To explore the regulatory outcomes of TfR1 from the view of whole transcriptome profile, we generated TFRC knockdown (TFRC-KD) HeLa cells and negative control (NC) cells using short hairpin RNA (shRNA) method. Unbiased transcriptome sequencing (RNA-seq) experiment was used to analyze the global expression level and alternative splicing (AS) changes between TFRC-KD and NC cells. Results: We found TFRC was consistently elevated in cervical cancer samples and tightly associated with prognosis of patients. Differential expression analysis revealed that 629 differentially expressed genes (DEGs) were identified between TFRC-KD and NC. Functional enrichment analysis of these DEGs revealed that TFRC-KD extensively disturbed cell physiology related pathways, including immunity, cell metabolism and gene expression. Moreover, dysregulated AS profile also indicated that TfR1 has important roles in the AS regulation. Hundreds of TfR1-regulated AS genes were involved in DNA repair, cell death, transcription and viral reproduction pathways, which were tightly associated with cancer cell progression. Conclusions: In summary, we for the first time explored the molecular functions of TfR1 at transcriptional and post-transcriptional levels. These results demonstrate TfR1 participates in the progression of cervical cancer by affecting the expression and AS levels of genes in cancer associated pathways, which greatly extends our understanding of TfR1 functions besides iron homeostasis and provide novel options in cancer treatment by targeting TfR1.

Influence of track irregularities in high-speed Maglev transportation systems

Huang, Jing Yu,Wu, Zhe Wei,Shi, Jin,Gao, Yang,Wang, Dong-Zhou Techno-Press 2018 Smart Structures and Systems, An International Jou Vol.21 No.5

Track irregularities of high-speed Maglev lines have significant influence on ride comfort. Their adjustment is of key importance in the daily maintenance of these lines. In this study, an adjustment method is proposed and track irregularities analysis is performed. This study considers two modules: an inspection module and a vehicle-guideway coupling vibration analysis module. In the inspection module, an inertial reference method is employed for field-measurements of the Shanghai high-speed Maglev demonstration line. Then, a partial filtering, integration method, resampling method, and designed elliptic filter are employed to analyze the detection data, which reveals the required track irregularities. In the analysis module, a vehicle-guideway interaction model and an electromagnetic interaction model were developed. The influence of the measured line irregularities is considered for the calculations of the electromagnetic force. Numerical integration method was employed for the calculations. Based on the actual field detection results and analysis using the numerical model, a threshold analysis method is developed. Several irregularities modalities with different girder end's deviations were considered in the simulations. The inspection results indicated that long-wavelength irregularities with larger girder end's deviations were the dominant irregularities. In addition, the threshold analysis of the girder end's deviation shows that irregularities that have a deviation amplitude larger than 6 mm and certain modalities (e.g., M- and N-shape) are unfavorable. These types of irregularities should be adjusted during the daily maintenance.

Screening of Differentially Expressed Genes Related to Bladder Cancer and Functional Analysis with DNA Microarray

Huang, Yi-Dong,Shan, Wei,Zeng, Li,Wu, Yang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8

Objective: The purpose of this study was to identify genes related to bladder cancer with samples from normal and disease cases by microarray chip. Methods: After downloading the gene expression profile GSE3167 from Gene Expression Omnibus database which includes 50 bladder samples, comprising 9 normal and 41 disease samples, differentially expressed genes were identified with packages in R language. The selected differentially expressed genes were further analyzed using bioinformatics methods. Firstly, molecular functions, biological processes and cell component analysis were researched by software Gestalt. Then, software String was used to search interaction relationships among differentially expressed genes, and hub genes of the network were selected. Finally, by using plugins of software Cytoscape, Mcode and Bingo, module analysis of hub-genes was performed. Results: A total of 221 genes were identified as differentially expressed by comparing normal and disease bladder samples, and a network as well as the hub gene C1QBP was obtained from the network. The C1QBP module had the closest relationship to production of molecular mediators involved in inflammatory responses. Conclusion: We obtained differentially expressed genes of bladder cancer by microarray, and both PRDX2 and YWHAZ in the module with hub gene C1QBP were most significantly related to production of molecular mediators involved in inflammatory responses. From knowledge of inflammatory responses and cancer, our results showed that, the hub gene and its module could induce inflammation in bladder cancer. These related genes are candidate bio-markers for bladder cancer diagnosis and might be helpful in designing novel therapies.