筋小胞體의 ATPase Kinetics에 關한 硏究

朴映淳,李承兩,朴泰水,庾基洙,李 弘,金允坤 圓光大學校 1982 論文集 Vol.16 No.2

본 연구에서는 토끼 골격근 소포체에서 분리한 근소포체의 ATPase활성에 대한 일반적 특성과 수종의 2가 양이온(??)의 영향에 관한 연구를 하였다. ATPase활성과 Ca uptake에 미치는 2가 양이온의 저해 효과와 저해기작은 Dixon plot, Lineweaver-Burk plot, Hanes-Woolf plot, Woolf-Augustinsson-Hofstee plot, Eadie-Scatchard plot, Arrhenius plot등을 이용하여 분석하였으며 그 결과는 다음과 같다. 1. 근소포체의 ATPase는 ??과 ??의 존재하에서 활성화 되어지며 ??의 농도가 4mM일 때 ??의 최적 농도는 0.1mM이었다. 2. 근소포체의 ATPase는 ??-ATPase와 (??)-ATPase로 구성되어 있고 (??) - ATPase의 활성은 ??-ATPase의 활성보다 높았다. 3. ATPase의 활성화 에너지는 약 19Kcal/mol이었다. 4. ATPase 활성에 미치는 2가 양이온은 12.5㎛과 400㎛의 범위 내에서 이온 농도가 증가됨에 따라서 활성도는 점점 감소되었다. 5. ATPase활성율 50% 저해시키는데 2가 양이온의 농도는 Ki값과 같아서 ??, ??, ?? Pd ??에 대한 각각의 농도는 10㎛, 30㎛, 130㎛ 및 350㎛이었다. 특히 ??은 저 농도에서 (12.5-100㎛)는 활성제로, 고농도에서 (200-400㎛)는 저해제로 작용하는 것 같다. 6. 2가 양이온은 ATPase의 Km에는 변화를 주지 않으나 Vmax의 저하를 일으키는 것으로 보아 비경쟁 저해제임이 판정되었다. 7. 2가 양이온은 가역적 저해제임이 판명되었다. 8. Ca 吸收能에 대한 CuCl₂의 阻害는 50~500㎛의 CuCl₂농도에서 현저히 나타났으며 이 현상은 Ca 吸收能과 ATPase 活性과 밀접한 관계를 보여주는 것이다. General properties of the ATPase and the effects of the divalent cations (??) on the total ATPase activity of the fragmented sarcoplasmic reticulum (FSR) isolated from rabbit skeletal muscle were investigated in the present resent research. The inhibitory effect of divalent cations on the FSR ATPase activity and its mechanism were analyzed by various plots such as Dixon plot, Lineweaver-Burk plot, Hanes-woolf plot, Eadie-Scatchard plot and Arrhenius plot. The results obtained were as follows: 1. The FSR ATPase is activated by ?? and ??; the optimum concentration of is 0.1 mM in the presence of 4 mM MgCl₂. 2. The FSR ATPase could be distinguished as ??-ATPase and (??)-ATPase. The activity of (??)-ATPase predomiant over that of ??-ATPase. 3. The apparent energies of activation for total ATPase was approximately 19Kcal/mole. 4. The inhibitory effects of the divalent cations on the enzyme activity increased as the concentrations of the divalent cations increased in the concentrations between 12.5 and 400㎛. 5. Concentration of 50% inhibition for each divalent cation was almost identical with the inhibition constant (ki). The Ki's were 10,30,130, and 350㎛ for ??, ??, ??, and ??, respectively. ?? seemed to be anactivator at lower concentrations and an inhibior at higher concentratios. 6. The divalent cations did not change the Km but decreased the Vmax of the FRS ATPase, suggesting that they are noncompetitive inhibitors on the FSR ATPase. 7. The divalent cations were also found to be revesible inhibitors on the FSR ATPase. 8. The inhibitory effect of CuCl₂on a uptake increased as the concentration CuCl₂increased in the range between 50㎛.

관동맥질환에서 저밀도 지단백 입자 크기의 분포

임효영,김인숙,승기배,강동헌,전두수,장기육,박준철,이수영,홍순조,최규보 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.8

Cs이 흡착된 Si(111)7×7표면에 대한 RHEED연구

박종윤,이순보,이경원,안기석,강건아 성균관대학교 1991 論文集 Vol.42 No.1

Cesium-adsorbed surface structures on Si(111)7×7 were investigated at room and high temperatures(200∼700℃) by RHEED. The RHEED patterns of Si(111) 7×7 was changed to the modified 7×7 and the 1×1 patterns with increasing the deposition times of Cs at RT. It was observed that the structure of the Cs-adsorbed Si (111) 7×7 surface at saturation coverage is the 1×1 structure at RT. The ◁그림삽입▷(원문을 참조하세요) 1 and 1×1 structures appeared successively at the adsorption temperature of 300℃, 350∼400℃ and 450℃, respectively. After subsequent heating of 1×1 surface above 550℃ and of ◁그림삽입▷(원문을 참조하세요) surface above 600℃, each RHEED pattern gradually returned to the original Si(111)7×7 pattern. 상온 및 200∼700℃의 Si(111)7×7 표면에 Cs(Cesium)을 증착하였을 때 표면격자구조의 변화를 RHEED로 관측하였다. Cs 증착시 Si(111)7×7 기판의 온도가 상온인 경우, 포화 덮임률에 도달했다고 추정되는 일정 증착시간 전에는 원래의 깨끗한 Si(111)7×7 패턴과 거의 유사한 변형된(modified) Si(111)7×7-Cs 패턴이 관측되었다. 그후 포화 덮임률에서는 1×1패턴이 관측되고 증착량을 증가시켜도 패턴의 변화는 관측되지 않았다. 이 구조를 다시 annealing시키면 약 550℃부터 서서히 원래의 7×7구조로 되돌아가기 시작한다. Si(111)7×7기판의 온도를 220∼700℃로 유지하면서 Cs을 증착시킨 경우에 약 250℃까지는 상온에서와 비슷한 변형된(modified) 7×7이 관측되고 약 300℃에서는 ◁그림삽입▷(원문을 참조하세요) 350∼400℃ 정도에서는 ◁그림삽입▷(원문을 참조하세요)과 3×1이 겹쳐셔 관측되었다. 그리고 450℃ 이상에서는 1×1구조가 관측되었다. 이때 약 300℃에서 형성된 ◁그림삽입▷(원문을 참조하세요) 350℃에서 형성된 ◁그림삽입▷(원문을 참조하세요) 3+3×1 구조는 약 500℃ 정도까지 다시 annealing함에 따라 다리 1×1구조로 상전이가 일어난후, 약 600℃부터 원래의 7×7의 초격자점들이 나타나기 시작했다. 이들 결과로부터 Si(111)7×7 표면에 Cs을 증착하는 경우에는 일정한 포화 덮임률(saturation coverage)이 있는 것으로 추정되고, 이 덮임률에서 관측된 고온에서의 상전이는 증착량(증착시간)에는 무관하고 온도에만 의존함을 알 수 있었다. 또한 1×1 구조와 ◁그림삽입▷(원문을 참조하세요) 구조에 대하여 Cs의 탈착은 각각 약 550℃와 600℃에서 일어나기 시작하여 700℃에서 완전히 탈착됨을 알 수 있었다.

K이 흡착된 Si(111)7×7표면에 대한 RHEED 연구

박종윤,이순보,이경원,안기석,신익조,강건아 성균관대학교 1990 論文集 Vol.41 No.2

상온 및 200℃∼600℃의 Si(111) 7×7 표면에 칼륨(K)을 증착하였을 때의 표면격자구조 변화를 RHEED로 관측하였다. K 증착시 Si(111)7×7 기판의 온도가 상온인 경우, 어느 일정한 증착시간(포화덮임률에 도달했다고 추정되는 증착시간)이 지난 후에는 원래의 깨끗한 Si(111)7×7 패턴과 유사한 Si(111) 7×7-K 패턴이 관측되었고, 증착시간을 증가시켜도 RHEED 패턴은 변화하지 않았다. 이것을 annealing하면 350℃까지는 RHEED 패턴에 변화가 없다가 그 이상의 온도가 되면 서서히 원래의 7×7 패턴으로 되돌아가기 시작한다. Si(111) 7×7기판의 온도를 200℃∼600℃로 유지하면서 K을 일정시간(450℃에서 3×1이 형성되는 증착시간)이상 증착시킨 경우에 250℃까지는 상온의 경우와 비슷한 형태의 변형된 7×7 패턴이 관측되고, 300℃∼550℃일때는 3×1, 550℃ 이상에서는 1×1 구조가 관측되었다. 이때 300℃∼550℃에서 형성된 Si(111) 3×1-K 구조는 450℃에서 1분 정도 annealing 하면 항상 Si(111) 1×1-K 구조로 상전이가 일어남을 관측하였다. Potassium adsorbed surface structures of Si(111) 7×7 surface at room and high temperatures(200℃∼600℃) were investigated by RHEED. Potassium adsorption on the Si(111) 7×7 surface to saturated coverage at room temperature changed the RHEED pattern of Si(111) 7×7 to Si(111) 7×7-K. Subsequent heating of the Si(111) 7×7-K surface above 350℃ results in a Si(111) 7×7 with desorbing K. The RHEED pattern of the K-adsorbed on the Si(111) 7×7 surface up to the adsorption temperature of 250℃ was the modified 7×7 pattern, quite similar to that of the Si(111) 7×7-K, observed at room temperature. The 3×1 structure was observed in the temperature of adsorption between 300℃ and 550℃. Regardless of the adsorption temperature, a phase transition always took place from the Si(111) 3×1-K structure to Si(111) 1×1-K after annealing at 450℃ over 1 minute.

一部 農村住民의 高血壓 危險要因에 對한 疫學的 硏究

박광희,김기순,안현옥,노희경 조선대학교 부설 의학연구소 1998 The Medical Journal of Chosun University Vol.23 No.2

To find risk factors of hypertension among farmers whose age were 30 or more living at a rural area in the suburbs of Kwangju, a case-control study was done toward hypertensive patients and age-sex matched controls. Case groups were composed of mild hypertensive group of 70 patients whose blood pressure were 140/90 mmHg or more and below 160/100mmHg, and moderate hypertensive group of 54 patients whose blood pressure were 160/100mmHg or more. As control groups same number of persons were chosen from normotensive patients by age and sex frequency matching. The results are as follows : 1. No significant differences of educational level, marriage state, occupation and monthly Living expenses were found between case and control groups. 2. Among the mild hypertensive group the odds ratio for drinking more than 50 gm of alcohol daily versus non-drinker was 5.06 (95% confidence interval 〔CJ〕1.39 - 18.38): odds ratio for persons with high score to take salty food versus persons with low score to take salty food was 2.45 (95% CI 1.23-4.88): odds ratio for persons who were at sitting position for more than :t. 5 hours a day versus persons who were at sitting position for less than 4.5 hours a day was 2.26 (95% CI 1.15-4.47) respectively. 3. Among the moderate hypertensive group a trend was found to increase the odds ratios for drinking alcohol 20- 39.9 gm versus non-drinker, high body mass index (BMI) versus low BMI, high waist-hip ratio (WHR) versus low WHR, persons who took more fatty food versus persons who took less fatty food, and for persons who slept less than 7 hours or more than 9 hours a day versus persons who slept for 7 - 9 hours a day. The odds ratios for persons who worked for more than 10 hours a day versus persons who worked for less than 10 hours a day showed 0.59. But the confidence interval values were not statistical1y significant. In conclusion excess drinking. high salt intake and inadequate activity were found to be the significant risk factor for mild hypertension of the farmers. So appropriate education to change the life stile are necessary.

요 침전물의 형태학적 변화에 관한 검토

박정오,윤기은,김순랑 서울保健大學 1996 論文集 Vol.16 No.1

We used midstream urine sedments from S. N. U. hospital patients in hematuria were examined with urine chemical analyzer. To better understand the bleeding mechanism of dysmorphic erythrocytes which might cause the nephron model in vitro. We have exposed the pH and osmolarity of suspension were systematically changed with in a range of pH 1 to 14 and 48 to 1484 mOsm/L. It was found that, our preliminary studies had suggested that morphological change of dysmorphic erythrocytes might still be to make it fully useful by phase-contrast microscopy and give a immediate answer or within 120 minutes. Futhermore, we could not confirmed by our criteria dysmorphism of their urinary erythrocytes. Such positive findings, although resulting from a small study, compel us to recommend this inexpensive method in the initial approach to patients with hematuria. In our opinion, it can greatly help the clinician in differentiating glomerular and non-glomerular bleeding from other cause of hematuria and channeling such patients towards the most appropriate Investigations.

全南地方의 水稻白葉枯病菌 菌型과 그의 地域別 및 品種別 分布

朴淳直,金基淸,禹洪斗,崔容壽 全南大學校 農漁村開發硏究所 1986 農業科學技術硏究 Vol.21 No.-

This was carried out to identify the pathotypes of Xanthomonas campestris pv. oryzae collected from regional exhibition paddys and others in Jeonnam province, and to investigate the sub-regional and varietal distributions of them in 1982. Collected isolates were identified as pathotypes Ⅰ, Ⅱ, Ⅲ and 2 unknown groups with Korean differentials; Ⅰ, Ⅱ, Ⅲ, Ⅳ,Ⅴ and 4 unknown with Japanese; Ⅰ, Ⅱ and 2 unknown with IPPI's, respectively. The fact that unknown groups were isolated suggest that some differentiation is occurring among the previous pathotypes. Using Japanese differentials, pathotype was isolated from l1 sub-regions, Ⅱ and gⅢfrom 4, g from I, g from 3, and unknown 4 groups from 8, respectively. However, there was no sub-region where all 5 pathotypes were detected. Pathotypes with Japanese differentials was isolated from 6 varieties, Iri 346 and 347, Milyang 23 and 30, Jinjubyo and Nakdongbyo: pathotype Ⅱ from 6 varieties, Suvreon 294, Irl 342 and 347, Jinjubyo and Akihikari; pathotype Ⅲ from 5 varieties, Suweon 294, Iri 346, Milyang 30 and Nongrim 41; pathotype Ⅳ from 1 variety, Jinjubyo; pathotype V from 4 varieties, Suweon 294, Iri 347, Milyang 23 and Jinjubyo, respectively.

조선업 근로자의 목부위 자각증상의 유병률과 관련 요인

박종,김기순,강명근,류소연,이철갑,강성득,김철호 朝鮮大學校 附設 醫學硏究所 2004 The Medical Journal of Chosun University Vol.29 No.1

Background and Objectives : This study examined prevalence and related factors of musculoskeletal symptoms on area of necks of shipbuilding workers in order to prevent musculoskeletal disorder and find out its managerial plans. Materials and Methods : this study targeted 1, 159 male shipbuilding workers who work for large suipbuilding companies, and interviewed them using organized questionnaire having short answer questions in February, 2003. Results: The prevalence of musculoskeletal symptoms (pains, anesthesia, and numbness) of the subjects was 16.0% on necks. As a result of multiple logistic regression analysis, the following results were obtained : sleeping hours, working departments, extra working hours, perceived amount of working, availability of dispensary were significantly related with symptoms on neck. When the variables that had significant relation with prevalence of neck area symptoms were analysed health behavior, it was found that the odds ratio of the group who had more than sufficient sleeping hours was 1.70 times higher than that of the group who had not. When they were examined in terms of occupational characteristics, the odds ratio of the subcontract company workers was 0.44 times lower than that of the parent company workers, and that of the group who had two hours of extra working hours was 1.78 times higher than that of the group who did not. The odds ratio of the group who thought the perceived working amount was slightly over the proper level was 1.49 time higher, and that of the group who thought it was excessively over the proper level was 3.21 times significantly higher. The odds ratio of the group who thought availability of dispensary was moderate was 1.84 times significantly higher than that of the group who thought it was easy. Conclusion : It was found that 16.0% of shipbuilding workers had musculoskeletal symptoms on neck, and their deom-sociological, occupational, and socio-psychological characteristics affected the symptoms. Therefore, to decrease musculoskeletal symptoms of shipbuilding workers, wording and socio-psychological condition should be improved by considering related factors of the workers.

공급량 배분기법을 이용한 갈수기 병렬저수지 해석

박기범,이순탁 한국환경과학회 2006 한국환경과학회지 Vol.15 No.3

가뭄기간의 저수지 운영방안에 관한 연구

박기범,이순탁 한국환경과학회 2004 한국환경과학회지 Vol.13 No.12

In this study the results of optimal water supply analysis by operating constraints of reservoirs during drought period are as follows. During drought period, water supply reliability is possible about 97~61% by CASE 1-CASE 5. Water supply reliability is possible about 97.3% in case of the Andong dam and 87.7% in case of the Imha dam by CASE 3. Also, under the constraints of CASE 4, water supply reliability is possible about 87.5% in case of the Andong dam and 73.3% in case of the Imha dam. The reason what low of available water supply ratio is decreased inflow of Imha dam. When compare standard deviation of average storage with standard deviation of storage, stable storage can be secured during successive drought period. And it also can minimize shortage of water during drought. therefore, it is impossible that reservoir supply sufficient water but change of operating condition is better than pervious on that followed by full reservoir level. It is need that the study for optimal water supply during drought period has to be continued.