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Aniline 을 분해하는 Flavimonas oryzihabitans 균주의 분리 , 동정 및 분해특성
홍성갑(Seong Karp Hong),박용근(Yong Keun Park),이영록(Yung Nok Lee) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.3
Novel strains degrading aniline were isolated from soils containing many herbicides in Kyonggi-Do, when aniline was provided as a sole carbon and nitrogen source. Among of them, three strains were identified as Flavimonas oryzihabitans KH1, KH2, and KH3. All of these three strains metabolized aniline through ortho-cleavage pathway. F. oryzihabitans KH1, KH2, and, KH3 strains showed narrow substrate-range in their ability on degrading various aromatic compounds containing aniline and catechol derivatives : they were not able to degrade chloroanilines and chlorocatechol , KH1 is superior to the other strains in view of catechol-1,2-dioxygenase activity of 0.42 unit/㎎ at 260 ㎚ absorbance and oxygen consumption of 300 nmol/min/㎎ in oxidizing aniline (2 mM) as a substrate. Complete degradation of aniline (2 mM) with KH1 was identified by GC-Mass analysis.
Electroencephalographic Signatures of Repetitive Motor Cortex Stimulation in Neuropathic Pain Rats
( Myeounghoon Cha ),( Seong-karp Hong ),( Un Jeng Kim ),( Young-hee Sa ),( Bae Hwan Lee ) 한국감성과학회 2017 한국감성과학회 국제학술대회(ICES) Vol.2017 No.-
Pain modulates rhythmic neuronal activity recorded by electroencephalography (EEG). Although these techniques have been used to study diseases such as cerebrovascular disorders, consciousness disorders or epilepsy, they have rarely been used in chronic pain diagnosis studies. In this study, we hypothesized that pain increases EEG power and rhythmical oscillations in rat models of chronic neuropathic pain, whereas treatments with daily motor cortex stimulation (MCS) reverse these changes. In order to quantify the EEG signal change, EEGs were recorded in chronic neuropathic pain state and compared with EEGs in individuals with pain relief by repetitive cerebral MCS method. Our results show increased brain rhythmical oscillations in awake, freely behaving rat models of chronic neuropathic pain. We found that in rats with neuropathic pain, ongoing activities were increased. However repetitive MCS modulated these brain oscillations when it relieved pain. In the EEGs of MCS treatments, the delta- and theta-frequency band decreased, while activities at alpha- and beta-frequency bands did not significantly change. These data suggest that quantitative EEG might be a valuable predictor. of pain and analgesia in rodents. This study was supported by the Basic Research Program through the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2015R1C1A1A01053484 and 2017R1A2B3005753)
Activities of Natural Plant Extracts against HIV-1
Eum, Jin-Seong,Park, Young-Doo,Hong, Seong-Karp The Korea Institute of Information and Commucation 2009 Journal of information and communication convergen Vol.7 No.4
Anti-HIV-1 activities for the extracts (buthanol, hexane, chloroform, and water) of medicinal plants widely used in the folk medicine were evaluated for screening of anti-AIDS agents. The activities of the extracts to inhibit HIV-1 replication were also analyzed. The 50% effective concentration (EC50) of inhibition activity of the p24 production for chloroform extract of Saphora flavescens, chloroform extract of Herba ephedrae, and hexane extract of Pachyma hoelen Rumph showed 5.8, 29.9, and 37.3 2g/ml, respectively, as good activities. Hexane extract of Sophora flavescens, buthanol extract of Tulipa edulis, hexane extracts of Tulipa edulis, Herba ephedra, and Pachyma hoelen Rumph in the 50% cytotoxic concentration ($CC_{50}$) in inhibition activities of recombinant HIV-1 RT showed 12.9, 19.5, 11.6, 12.0, and 36.8 % at concentration of 200 ${\mu}g$/ml, respectively, as good activities. From these results, chloroform extract of Saphora flavescens, chloroform extract of Herba ephedrae, and hexane extract of Pachyma hoelen Rumph were very effective against HIV-1 among all extracts tested. Therefore, we expect these plants will be a useful for anti- HIV-1 therapeutics in future.
Kim, Bum-Joon,Hong, Seong-Karp,Lee, Keun-Hwa,Yun, Yeo-Jun,Kim, Eui-Chong,Park, Young-Gil,Bai, Gil-Han,Kook, Yoon-Hoh American Society for Microbiology 2004 Journal of clinical microbiology Vol.42 No.3
<P>A novel duplex PCR method that can amplify the 235- and 136-bp rpoB DNAs of Mycobacterium tuberculosis complex and nontuberculous mycobacteria (NTM), respectively, with two different sets of primers was used to differentially identify 44 reference strains and 379 clinical isolates of mycobacteria in a single-step assay. Showing 100% sensitivity and specificity, the duplex PCR method could clearly differentiate M. tuberculosis complex and NTM strains. In addition, restriction fragment length polymorphism analysis and direct sequencing of the amplicon of NTM could be used to supplement species identification.</P>
Efficacy of Gene Transfer and Expression of Novel Recombinant Baculovirus Vector
권태동,홍성갑,Kweon, Tae-Dong,Hong, Seong-Karp The Korea Institute of Information and Commucation 2014 한국정보통신학회논문지 Vol.18 No.8
폴리히드론 프로모터, 수포성구내염 바이러스G, 폴리A, 사이토메가바이러스 프로모터, 강화녹색형광단백질, 단백질전달부위 유전자 등을 포함한 새로운 베큘로바이러스 시스템이 제조되었다. 본 재조합벡터 시스템은 인간 섬유아세포에 적용하여 시험하였고 재조합된 유전자의 전달과 유전자 발현을 대조 벡터시스템과 비교하였다. 본 연구로부터 새롭게 제작된 본 베큘로바이러스 시스템이 유전자의 전이와 유전자 발현 면에서 대조 벡터시스템 보다 고효율을 나타내었다. Novel baculovirus vector systems recombined with coding genes of polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP), and protein transduction domain (PTD) were constructed. These recombinant baculovirus vector systems were applied into human foreskin fibroblast cells and compared the effects of gene transfer and gene expression of these recombinant baculovirus vector systems with control vector system. From this study, it showed that these novel recombinant baculovirus vector systems were superior efficacy to control vector system in view of gene transfer and gene expression.
Generation of Demyelination through Use of M. leprae-specific phenolic glycolipid-1 (PGL-1)
Kim, Ji-Young,Choi, Chang-Shik,Hong, Seong-Karp Korean Society of Photoscience 2015 Rapid communication in photoscience Vol.4 No.2
For myelination, Schwann cells and neuron cells from dorsal root ganglion (DRG) of rat embryos (E16) were cultured in vitro system. The purified DRG cells with anti-mitotic agents and purified Schwann cells were cocultured and then accomplished myelination processing. Treatment of M. leprae-specific phenolic glycolipid-1 (PGL-1) into this coculture system was performed and then accomplished demyelination. Therefore, we identified demyelination processing using antibody of myelin basic protein (MBP).