Chlorella Mitochondria의 Oxidative Phosphorylation에 대한 Ascorbic Acid 및 IAA의 작용성에 관하여

이영록,진평,Lee, Yung-Nok,Chin, Pyung 한국미생물학회 1964 미생물학회지 Vol.2 No.1

Lee, Yung Nok and Chin, Pyung (Dept. of Biology, Korea University, Seoul, Korea) : Action of ascorbic acid indoleacetic acid on the oxidation of succinate and coupled phosphorylation in Chlorella mitochondria. Kor. Jour. Microbiol., Vol.2, No.1, p12-16 (1964) Mitochondria were isolated from Chlorella ellipsoidea and the action of ascorbic acid and indoleacetic acid on the succinate oxidation and coupled phosphorylation in mitochondria suspension were examined. Oxidation of succinate used as substrate, and phosphorylation coupled to oxidation were strikingly enhanced by the addition of ascorbic acid, while in case of indoleacetic acid it were a little. In a view of phosphorylative efficiency, P/O ratio resulting from the addition of ascorbic acid was decreased and it may be considered as the result of a partial oxidation of ascorbate in mitochondria.

Chlorella 의 물질대사에 미치는 미양원소의 결핍효과 1 (제 1 ) -생 및 광합성 에 관하여-

이영록,진평,심웅섭,Lee, Yung-Nok,Chin, Pyung,Sim, Woong-Seop 한국미생물학회 1967 미생물학회지 Vol.5 No.1

Chlorella ellipsoidea cells were cultured in an iron, copper, zinc, manganese, molybdenum or boron-free medium. Physiological activities such as growth rate, reproduction, endogenous and glucose respiration, photosynthetic activity and biosythesis of chlorophyll of the micro-element definition cells were measured. It generally, growth rate, respiratory and photosynthetic activities, and biosynthesis of chlorophyll of the micro-element deficient cells decreased more or less, compared with those of the normal cells. The growth of the algal cells in an iron-free medium were retarded severely with the chlorosis, and the photosynthetic activity of the cells decreased remarkably even though the low content of chlorophyll in the cells owing to the iron-deficiency is considered. Therefore, it is deduced that iron takes part in the photosynthetic process itself, possibly by its participation in the photo phosphorylation coupled with electron transport. Respiratory activity of boron-deficient cells showed the most severe decrease whereas those of the molybdenum-deficient cells showed very slight decrease in spite of severe growth retardation.

Chlorella 의 물질대사에 미치는 미양원소의 결핍효과(제 2 ) -, 리보 및 의 생합성능에 관하여-

이영록,진평,심웅섭,Lee, Yung-Nok,Chin, Pyung,Sim, Woong-Seop 한국미생물학회 1968 미생물학회지 Vol.6 No.1

Chlorella ellipsoidea cells were cultured in an iron, copper, zinc, manganese, molybdenum or boron-free medium. Biosynthetic activities of nucleic acids, protein and phospholipid in chlorella cells, which were growing in a microelement deficient medium were compared with those of the normal cells by measuring the contents of phosphate, amino acids or UV-absorbing substances in the various cell fractions. When the algae were grown in a molybdenum-free medium, the amounts of phosphate in the acid-soluble fraction of the cells increased, whereas the amounts of alkali-stable protein and RNA decreased compared with the normal cells showing that the synthesis of protein and RNA from the early products of photosynthesis was inhibited. When the algae were grown in a boron-free medium, amounts of alkali-labile protein and phospholipid of the cells decreased, while the amount of phosphate in acid-soluble fraction increased compared with the normal cells showing that the biosynthesis of protein and phospholipid from the early products of photosynthesis was retarded. In general, amounts of protein and RNA in the microelement deficient cells significantly decreased compared with those of the normal cells. Phosphate content in the acid-soluble fraction of the algal cell grown in an zinc, copper, molybdenum, or boron-free medium increased considerably, whereas that of the algal cell grown in an iron or manganese-free medium decreased remarkably compared with that of the control. It is considered, therefore, that molybdenum, zinc, copper and boron etc. play an important role in the biosyntbesis of macromolecule from acid-soluble phosphate compounds, in contrast to the principal action of iron and manganese on the photosynthetic process itself.

$^{32}P$-Labeled Chlorella의 정상배지에 있어서의 $^{32}P$ 및 Total P의 전환

이영록,진평,Lee, Yung-Nok,Chin, Pyung 한국미생물학회 1966 미생물학회지 Vol.4 No.1

1. Uniformly $^{32}P$-labeled Chlorella cells were further grown in a standard "cold" medium and aliquots of the algal cells were taken out at the beginning of, and at intervals during the culture, and subjected to analyze the contents of $^{32}$ P and total P in various fractions of the cell constituents. 2. When the $^{32}P$--labeled algae were grown in a normal "cold" medium, the P-contents in the fractions of DNA and protein increased. In the meantime the $^{32}P$- in acid-insoluble polyphosphate fraction decreased considerably, while that in RNA-polyphosphate complex significantly increased. 3. It was inferred that, under the experimental conditions of the present study, the phosphorus in polyphosphate seems to be transferred to RNA polyposphate complex and the phosphorus used in the synthesis of DNA and protein was, directly or indirectly, taken from those fractions above.ose fractions above.

Chlorella의 유기산대사에 관한 연구

진평,이영록,Chin, Pyung,Lee, Yung-Nok 한국미생물학회 1965 미생물학회지 Vol.3 No.2

Using the synchronous culture method and the manometric technique, changes in respiratory activities, utilization of some organic acids (succinate, malate, lactate and acetate etc.) and its effect on glucose metabolism in Chlorella cells at different growing stages were measured. 1) Endogenous respiration of the cells was not active at growing stage and was almost constant throughout the early ripening, maturing and division stages. 2) Lactate was utilized as respiratory substrate better than other organic acids tested. Exogenous respiration of glucose was most active at growing and maturing stages and was decreased strikingly at division stage. 3) Succinate and citrate inhibited endogenous and glucose respiration of the cells throughout the all life cycle. 4) Malate and acetate were utilized in the cells at early growing and division stages better, and malate enhanced the glucose respiration while in case of acetate it was depressed. 5) Calcium ion inhibited not only permeability of respiratory substrate but endogenous respiration itself.

탄화수소를 자화하는 Pseudomonas의 분리동정

김정국,이영록,Kim, Jeong-Kook,Lee, Yung-Nok 한국미생물학회 1984 미생물학회지 Vol.22 No.1

서울 근교에서 채취한 河川水 및 土壤標品으로부터 238菌株의 탄화수소자화세균을 分離하였다. 分離된 菌株중 68菌株는 Pseudomonas屬으로 同定되었고, 11菌株는 Alcaligenes屬으로, 10菌株는 Acinetobacter屬으로 同定되었다. 68菌株의 Pseudomona중, 35菌株는 P. aeruginosa, 5菌株는 P. fluorescence, 10菌株는 P. putida, 그리고 2菌株는 P. mendocina로 각각 同定되었다. 238 strains of bacteria were isolated from sewage and soil samples collected mainly in Seoul and its suburbs by enrichment culture on crude oil or hydrocarbon minimal medium. Of the isolates, 68 strains were tentatively identified as the genus Pseudomonas, 11 strains as Alcaligenus, and 10 strains as Acinetobacter. Of the 68 strains of Pseudomonas sp., 35 strains were identified as P. aeruginosa, 5 strains as P. fluorescence, 10 strains as P. putida, and 2 strains as P. mendocina.

Aspergillus phoenicis의 生活史를 통한 Cellulase 및 Xylanase의 活性 변화

오찬석,이영록,Oh, Chan-Seok,Lee, Yung-Nok 한국미생물학회 1984 미생물학회지 Vol.22 No.2

The changes in the activities of Cellulase and Xylanase of A. phoencis during the life cycle were surveyed by using synchronized culture technique. Avicelase activity of the fungus was reached at peak in the initial hyphal growth stage, but decreased gradually during the conidiophore formation, vesicle-phialide formation, and sporulation stages in decreasing order. CMCase, Salicinase, and Xylanase activities were raised very high in the initial hyphal growth stage, but decreased gradually in conidiophore formation and vesicle-phialide formation stages, and then increased, more or less, in the sporulation stage.

안양천에서 분리한 수은 내성세균의 동정과 특성

유경만,조남륜,이원희,이영록,박용근 ( Kyung Man You,Nam Ryun Cho,Won Hee Lee,Yung Nok Lee,Yong Keun Park ) 한국환경생물학회 1995 환경생물 : 환경생물학회지 Vol.13 No.2

Twelve mercury-resistant bacterial strains were isolated from Anyangcheon. The mercury-resistant bacteria grown on the LB agar supplemented with 100㎍/㎖ of mercuric chloride were identified as Pseudomonas sp. and Bacillus sp.. All of the mercury-resistant bacteria exhibited resistance to at least one antibiotic. Among them, PY109. PY110, and PY112 were broad-spectrum strains which showed resistance to not only inorganic mercuric compound such as mercuric chloride(HgCl_2). but also organomercurial compound such as phenyl mercuric acetate (PMA). The remaining nine were narrow-spectrum strains resistant only to inorganic mercuric compound. PY102, PY103, and PY113 were resistant to cadmium(Cd^2+) as well as mercury(Hg^2+). Plasmids were detected in all strains of Pseudomonas sp., while all strains of Bacillus sp. except for PY106 had no plasmid. Growth of Pseudomonas sp. PY109 in the presence of mercury started after an extended lag phase. The growth curve of Pseudomonas sp. PY109 dropped to the lowest level as soon as the bacteria came into contact with HgCl_2 and PMA. However, The bacteria preincubated with a low concentration of HgCl_2 were prevented from early death. The decrease of concentration of Hg^2+ in the culture medium during growth of Pseudomonas sp. PY109 was confirmed by the UV-VIS spectrophotometer and the inductively coupled plasma atomic emission spectrophotometer.

Salmonella typhimurium 에서 무산소 환경 유도유전자들에 대한 조절유전자들의 특성 및 클로닝

이윤정,방일수,이인수,이영록,박용근 ( Yun Joung Lee,Iel Soo Bang,In Soo Lee,Yung Nok Lee,Yong Keun Park ) 한국환경생물학회 1993 환경생물 : 환경생물학회지 Vol.11 No.2

New regulatory loci which participate in the anaerobic environment inducible gene(ani) expression in Salmonella typhimurium were identified. These regulators (oxr) showed negative regulatory effect on the anaerobiosis inducible operon fusions of this organism in aerobic environment (oxr 107 and oxr 108 to ani 2001, oxr 109 to ani 2007). They were identified as the trans-acting regulators, and increased β-galactosidase activity of ani-lacZ fusion strains up to about 10-240 fold in aerobic environment. By using the library of S. typhimurium chromosomal DNA, two negative regulators (oxr 101 and oxr 109) were cloned. The insert fragment of each clone, pPK32 for oxr 101 and pPK33 to oxr 109 were 7.8Kb and 9.3Kb, respectively. The recombinant plasmid pPK32 didn`t complement the mutation effect of oxr 101 on minimal media but rich media, pPK33 complemented the mutation effect of oxr 109 on both minimal and rich media.

Aniline 을 분해하는 Flavimonas oryzihabitans 균주의 분리 , 동정 및 분해특성

홍성갑(Seong Karp Hong),박용근(Yong Keun Park),이영록(Yung Nok Lee) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.3

Novel strains degrading aniline were isolated from soils containing many herbicides in Kyonggi-Do, when aniline was provided as a sole carbon and nitrogen source. Among of them, three strains were identified as Flavimonas oryzihabitans KH1, KH2, and KH3. All of these three strains metabolized aniline through ortho-cleavage pathway. F. oryzihabitans KH1, KH2, and, KH3 strains showed narrow substrate-range in their ability on degrading various aromatic compounds containing aniline and catechol derivatives : they were not able to degrade chloroanilines and chlorocatechol , KH1 is superior to the other strains in view of catechol-1,2-dioxygenase activity of 0.42 unit/㎎ at 260 ㎚ absorbance and oxygen consumption of 300 nmol/min/㎎ in oxidizing aniline (2 mM) as a substrate. Complete degradation of aniline (2 mM) with KH1 was identified by GC-Mass analysis.