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Alterations of Epidermal Lipid Profiles and Skin Microbiome in Children With Atopic Dermatitis
Kim Jihyun,Kim Byung Eui,Goleva Elena,Berdyshev Evgeny,배재웅,Kim Seokjin,Kim Hye-young,Lee Un Ha,Kim Myoung Shin,Jung Minyoung,Kim Hyunmi,Lee Jinyoung,Donald Y.M. Leung,Ahn Kangmo 대한천식알레르기학회 2023 Allergy, Asthma & Immunology Research Vol.15 No.2
Purpose: We aimed to investigate epidermal lipid profiles and their association with skin microbiome compositions in children with atopic dermatitis (AD). Methods: Specimens were obtained by skin tape stripping from 27 children with AD and 18 healthy subjects matched for age and sex. Proteins and lipids of stratum corneum samples from nonlesional and lesional skin of AD patients and normal subjects were quantified by liquid chromatography tandem mass spectrometry. Skin microbiome profiles were analyzed using bacterial 16S rRNA sequencing. Results: Ceramides with nonhydroxy fatty acids (FAs) and C18 sphingosine as their sphingoid base (C18-NS-CERs) N-acylated with C16, C18 and C22 FAs, sphingomyelin (SM) N-acylated with C18 FAs, and lysophosphatidylcholine (LPC) with C16 FAs were increased in AD lesional skin compared to those in AD nonlesional skin and that of control subjects (all P < 0.01). SMs N-acylated with C16 FAs were increased in AD lesional skin compared to control subjects (P < 0.05). The ratio of NS-CERs with long-chain fatty acids (LCFAs) to short-chain fatty acids (SCFAs) (C24-32:C14-22), the ratio of LPC with LCFAs to SCFAs (C24-30:C16-22) as well as the ratio of total esterified omega-hydroxy ceramides to total NS-CERs were negatively correlated with transepidermal water loss (rho coefficients = −0.738, −0.528, and −0.489, respectively; all P < 0.001). The proportions of Firmicutes and Staphylococcus were positively correlated to SCFAs including NS ceramides (C14-22), SMs (C17-18), and LPCs (C16), while the proportions of Actinobacteria, Proteobacteria, Bacteroidetes, Corynebacterium, Enhydrobacteria, and Micrococcus were negatively correlated to these SCFAs. Conclusions: Our results suggest that pediatric AD skin shows aberrant lipid profiles, and these alterations are associated with skin microbial dysbiosis and cutaneous barrier dysfunction.
Hyojoong Kim,Minyoung Kim,Kim A. Hoelmer,Seunghwan Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05
The soybean aphid, Aphis glycines Matsumura, was recently introduced from Asia into North America (NA) where it has become a serious pest of soybeans. This invasive pest has rapidly spread throughout the midwestern United States and southern Canada since 2000. We examined 585 individuals obtained from 23 different collections in USA, Korea, China, and Japan using eight microsatellite loci. Based on analysis of multilocus genotype, gene diversity and number of alleles in NA were averaging 0.40 and 2.70, whereas in Asia averaging 0.55 and 4.32, respectively. The factorial correspondence analysis displayed that some Korean populations were closely related to the NA populations. Structure analysis resulted in two conspicuous clusters, NA and Asia, as the most likelihood number of clusters (K). Bayesian assignment tests revealed that Osan and Milyang populations were most likely assigned to the NA populations. Bottleneck test did not show significance of genetic bottleneck in all populations. We also discuss the invasive history of the soybean aphid in light of population genetics.
Jongok Lim,Minyoung Kim,Sunghoon Jung,Il-kwon Kim,Jong-su Lim,Shin-young Park,Gyeong-mi Kim,Cheolhak Kim,Bong-kyu Byun,Bong-woo Lee,Seunghwan Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.10
Larval stages of Callipogon relictus (Semenov) (Coleoptera: Cerambycidae), a gigantic longhorn beetle designated as a natural monument of Korea, has never been studied as it is hardly discovered in nature. The DNA barcoding gene, mt-COI, was used to identify a dead larva found in the Gwangneung forest of the Korea National Arboretum. Based on the result, we provide the morphology of the immature stage, with the illustrations of diagnostic characteristics.
Effect of Nd:YAG laser irradiation on adherence of retrograde filling materials
Minyoung Kim(김민영),Hyeon-cheol Kim(김현철),Sang Won Kwak(곽상원),Tai Cheol Yoon(윤태철),Euiseong Kim(김의성) 대한치과의사협회 2016 대한치과의사협회지 Vol.54 No.11
Background/Purpose: The purpose of this study was to evaluate the effect of Nd:YAG irradiation on adherence of retrograde filling materials (mineral trioxide aggregate [MTA] and Super-EBA) by micro-computed tomography (CT) measurement and to observe the dentinal surface after irradiation by scanning electron microscopy (SEM). Materials and methods: Forty retrofilling models using extracted human teeth were divided into four groups according to the material and method used: ProRoot MTA (MTA group), Super-EBA (EBA group), MTA with Nd:YAG laser irradiation (LMTA group), and Super-EBA with Nd:YAG laser irradiation (LEBA group). All specimens were stored in 100% humidity for 24 hours until micro-CT was performed. The gap volume of the tooth/material interface was measured using the CTAn program. In six samples, the laser-irradiated dentin surface was observed using SEM. Results: The mean percent difference in gap volume was not statistically significant between the Nd:YAG laser-irradiated groups and non-irradiated in both materials(P > 0.05). The gap volume in the MTA group was significantly lower than that in the EBA group (P < 0.05). Examination of the non-irradiated specimens by SEM showed patent dentinal tubules. In contrast, alterations in the texture of the dentin surface and obliteration of the dentinal tubules were evident in the Nd:YAG laser-irradiated specimens. Conclusion: In this study, changes in the dentinal surface after Nd:YAG irradiation did not affect adherence between the apical filling material and the dentin wall.
YS Flora®, a Comprehensive and Multi-Purpose Collection of Human Gut Microbiome
Minyoung Hong(Minyoung Hong),Sooyoon Cho(Sooyoon Cho),Kyoung Jin Choi(Kyoung Jin Choi),Gwanghee Kim(Gwanghee Kim),Sang Sun Yoon(Sang Sun Yoon) 한국유산균프로바이오틱스학회 2023 Current Topic in Lactic Acid Bacteria and Probioti Vol.9 No.2
Human feces, as a repository of the gut microbiome composed of tens of trillions of microbes, represent the microbial environment within the gut. The composition of the gut microbiota is associated with various diseases, body conditions, and lifestyles, and human-derived strains isolated from feces can be developed into probiotics or live biotherapeutic products (LBPs). YS Flora® is a collection of human fecal samples from over 300 diverse donors intended for scientific research and healthcare purposes, such as studying gut microbiome communities and developing supplements and pharmaceuticals. YS Flora® uniquely includes fecal samples from non-healthy individuals, unlike other fecal biobanks, to gain a comprehensive understanding of the human gut microbiome. To demonstrate YS Flora®'s utility for scientific research, we analyzed and compared the fecal bacterial communities of vegetarians and omnivores. Strains with potential for use as probiotics or LBPs, such as Lactobacillus spp., Bifidobacterium spp., and Bacillus spp., were isolated using selective media. YS Flora® provides a research foundation by offering isolated strains and gut microbiota from individuals with specific health conditions and lifestyles, enhancing the scientific understanding of the human gut microbiome and aiding researchers in developing probiotics or LBPs.
KIM, Mijung,KIM, Yongsun,LEE, Seunghoon,KUK, Minyoung,KIM, Ah Young,KIM, Wanhee,KWEON, Oh-Kyeong The Japanese Society of Veterinary Science 2016 The Journal of veterinary medical science Vol.78 No.4
<P>Allogenic adipose-derived mesenchymal stem cells (Ad-MSCs) are an alternative source for cytotherapy owing to their antioxidant and anti-inflammatory effects. Frozen-thawed allogenic Ad-MSCs can be used instantly for this purpose. However, the viability and function of frozen-thawed Ad-MSCs have not been clearly evaluated. The purpose of this study was to compare the viability and function of Ad-MSCs and heme oxygenase-1 (HO-1)-overexpressed Ad-MSCs <I>in vitro</I> after freeze-thawing. The viability, proliferation, antioxidant capacity and mRNA gene expression of growth factors were evaluated. Frozen-thawed cells showed significantly lower viability than fresh cells (77% for Ad-MSCs and 71% for HO-1 Ad-MSCs, <I>P</I><0.01). However, the proliferation rate of frozen-thawed Ad-MSCs increased and did not differ from that of fresh Ad-MSCs after 3 days of culture. In contrast, the proliferation rate of HO-1-overexpressed Ad-MSCs was lower than that of Ad-MSCs. The mRNA expression levels of <I>TGF-β</I>, <I>HGF</I> and <I>VEGF</I> did not differ between fresh and frozen-thawed Ad-MSCs, but <I>COX-2</I> and <I>IL-6</I> had significantly higher mRNA expression in frozen cells than fresh cells (<I>P</I><0.05). Fresh Ad-MSCs exhibited higher <I>HO-1</I> mRNA expression than frozen-thawed Ad-MSCs, and fresh HO-1-overexpressed Ad-MSCs exhibited higher than fresh Ad-MSCs (<I>P</I><0.05). However, there was no significant difference between fresh and frozen HO-1-overexpressed Ad-MSCs. The antioxidant capacity of HO-1-overexpressed Ad-MSCs was significantly higher than that of Ad-MSCs. Cryopreservation of Ad-MSCs negatively affects viability and antioxidant capacity, and HO-1-overexpressed Ad-MSCs might be useful to maximize the effect of Ad-MSCs for cytotherapy.</P>
Infrared Estimation of Canopy Temperature as Crop Water Stress Indicator
Kim, Minyoung,Kim, Seounghee,Kim, Youngjin,Choi, Yonghun,Seo, Myungchul Korean Society of Soil Science and Fertilizer 2015 한국토양비료학회지 Vol.48 No.5
Decision making by farmers regarding irrigation is critical for crop production. Therefore, the precision irrigation technique is very important to improve crop quality and yield. Recently, much attention has been given to remote sensing of crop canopy temperature as a crop water-stress indicator, because it is a scientifically based and easily applicable method even at field scales. This study monitored a series of time-variant canopy temperature of cucumber under three different irrigation treatments: under-irrigation (control), optimal-irrigation, and over-irrigation. The difference between canopy temperature ($T_c$) and air temperature ($T_a$), $T_c-T_a$, was calculated as an indicator of cucumber water stress. Vapor pressure deficit (VPD) was evaluated to define water stress on the basis of the temperature difference between leaf and air. The values of $T_c-T_a$ was negatively related to VPD; further, cucumber growth in the under- and over-irrigated fields showed water stress, in contrast to that grown in the optimally irrigated field. Thus, thermal infrared measurements could be useful for evaluating crop water status and play an important role in irrigation scheduling of agricultural crops.
( Minyoung Kim ),( Jae-gook Shin ),( Dong-hyun Kim ),( Eun-young Kim ),( Jong-lyul Ghim ),( Ji- Hye Kim ) 대한결핵 및 호흡기학회 2019 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.127 No.-
Backgrouod: Chemotherapy of multidrug-resistant tuberculosis is prescribed in combination for at least 2 years. According to WHO guideline, at least 5 drugs are simultaneously administered for successful therapy. TDM could be a useful tool for successful clinical practice. In this regards, a high-throughput analytical tool for the simultaneous determination of 24 anti-tuberculosis drugs covering all anti-TB drugs currently prescribed for the treatment of tuberculosis. Method: Two protein precipitation methods were adopted; one with methanol containing 0.13 N HCl for amikacin, kanamycin and streptomycin (group 1) and the other with acetonitrile for amoxicillin, bedaquiline, ciprofloxacin, clarithromycin, clofazimine, cycloserin, delamanid, DM-6705, ethambutol, ethionamide, isoniazid, levofloxacin, linezolid, moxifloxacin, PAS, prothionamide, pyrazinamide, rifabutin, rifampin, rifapentine and roxithromycin (group 2). Separation was done either on an HILIC silica column with a gradient elution (group 1) or a reversed-phase dC18 column with a gradient elution (group 2). Detection was carried out in selected MRM mode. Results: All drugs were well separated with high specificity. The calibration curves were linear over a 50-fold concentration range, with correlation coefficients (r) greater than 0.99 for all drugs. The limits of quantification were between 0.01 and 2.0 μg/ml. The intra- and inter-day precision was less than 14.3%, and the accuracy was between 85.7 and 109.5%. The recovery was more than 25% with RSD less than 16.0%. Most of the tested drugs were stable after 24 hours at room temperature, Freeze Thaw cycles, 12 hours of post-treatment. Delamanid was spontaneously degraded into the metabolite DM- 6705 and stable only within 5 hours at ice condition. Conclusion: This report describes the development and validation of the method for the simultaneous quantification of 24 anti-tuberculosis agents in human plasma by LC-MS/MS. The validated method is simple, fast, accurate, and precise. This assay can be applied to therapeutic drug monitoring in tuberculosis patients.