YS Flora®, a Comprehensive and Multi-Purpose Collection of Human Gut Microbiome

Minyoung Hong(Minyoung Hong),Sooyoon Cho(Sooyoon Cho),Kyoung Jin Choi(Kyoung Jin Choi),Gwanghee Kim(Gwanghee Kim),Sang Sun Yoon(Sang Sun Yoon) 한국유산균프로바이오틱스학회 2023 Current Topic in Lactic Acid Bacteria and Probioti Vol.9 No.2

Human feces, as a repository of the gut microbiome composed of tens of trillions of microbes, represent the microbial environment within the gut. The composition of the gut microbiota is associated with various diseases, body conditions, and lifestyles, and human-derived strains isolated from feces can be developed into probiotics or live biotherapeutic products (LBPs). YS Flora® is a collection of human fecal samples from over 300 diverse donors intended for scientific research and healthcare purposes, such as studying gut microbiome communities and developing supplements and pharmaceuticals. YS Flora® uniquely includes fecal samples from non-healthy individuals, unlike other fecal biobanks, to gain a comprehensive understanding of the human gut microbiome. To demonstrate YS Flora®'s utility for scientific research, we analyzed and compared the fecal bacterial communities of vegetarians and omnivores. Strains with potential for use as probiotics or LBPs, such as Lactobacillus spp., Bifidobacterium spp., and Bacillus spp., were isolated using selective media. YS Flora® provides a research foundation by offering isolated strains and gut microbiota from individuals with specific health conditions and lifestyles, enhancing the scientific understanding of the human gut microbiome and aiding researchers in developing probiotics or LBPs.

Low-Cost Cultivation and Sporulation of Alkaliphilic Bacillus sp. Strain AK13 for Self-Healing Concrete

( Minyoung Hong ),( Wonjae Kim ),( Woojun Park ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.12

The alkaliphilic, calcium carbonate precipitating Bacillus sp. strain AK13 can be utilized in concrete for self-repairing. A statistical experimental design was used to develop an economical medium for its mass cultivation and sporulation. Two types of screening experiment were first conducted to identify substrates that promote the growth of the AK13 strain: the first followed a one-factor-at-a-time factorial design and the second a two-level full factorial design. Based on these screening experiments, barley malt powder and mixed grain powder were identified as the substrates that most effectively promoted the growth of the AK13 strain from a range of 21 agricultural products and by-products. A quadratic statistical model was then constructed using a central composite design and the concentration of the two substrates was optimized. The estimated growth and sporulation of Bacillus sp. strain AK13 in the proposed medium were 3.08 ± 0.38 × 10⁸ and 1.25 ± 0.12 × 10⁸ CFU/ml, respectively, which meant that the proposed low-cost medium was approximately 45 times more effective than the commercial medium in terms of the number of cultivatable bacteria per unit price. The spores were then powdered via a spray-drying process to produce a spore powder with a spore count of 2.0 ± 0.7 × 10⁹ CFU/g. The AK13 spore powder was mixed with cement paste, yeast extract, calcium lactate, and water. The yeast extract and calcium lactate generated the highest CFU/ml for AK13 at a 0.4:0.4 ratio compared to 0.4:0.25 (the original ratio of the B4 medium) and 0.4:0.8. Twenty-eight days after the spores were mixed into the mortar, the number of vegetative cells and spores of the AK13 strain had reached 10⁶ CFU/g within the mortar. Cracks in the mortar under 0.29 mm were healed in 14 days. Calcium carbonate precipitation was observed on the crack surface. The mortar containing the spore powder was thus concluded to be effective in terms of healing micro-cracks.

Modeling the effects of climate change on insect pest-natural enemy systems using population dynamics and species interaction strength

Minyoung Lee,Jinsol Hong,Yongeun Kim,Hyoung-ho Mo,Kijong Cho 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04

Global warming can seriously influence on the interaction between pest and natural enemy in the agroecosystem due to the differences in optimal temperature ranges. Two aphid-ladybug systems, Myzus persicae-Coccinella septempunctata (M-C) and Aphis gossypii-Coccinella septempunctata (A-C) in the pepper crop were simulated, respectively under four different temperature scenarios including crop development over 244 days with the assumption that the average temperature is higher by 1, 3, and 5 °C than that in Seoul in 2000. Temperature-dependent functions for each aphid-ladybug system were embedded in Rosenzweig-Macathur predator-prey model to explore their population dynamics, and then Dynamic Index was used to quantify the strengths of species interactions. The result shows that the predator-prey population cycles as well as species interactions are getting shorter and stronger in both systems as temperature increased. Especially, the excessively high temperature scenario in Aphis gossypii-Coccinella septempunctata system could result in the extreme species interaction. Therefore, the increasing temperature can facilitate the effectiveness of biological control because of growing crop plant development and much stronger species interaction, although there are increases of the frequency of pest occurrences.

Lepidopteran Hitchhikers through Vessel of Inanimate Pathways

Minyoung Kim,Ik-Hwa Hyun,Ki-Jeong Hong,Heung-Sik Lee,Jong-Ho Lee,Seunghwan Lee 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

Lepidopteran hitchhikers were investigated in vessel at five major ports of Korea in 2013. These moths were collected by International Plant-Quarantine Accreditation Board (IPAB) during inspection on vessels for Asian Gypsy Moth (AGM). The results typically include the taxonomic identification, their origin localities, and information related to commodities and ship’s route. A total of 1,026 individuals were detected in vessels and identified with 236 species belonging to 21 families. Although most of the species are already known to the Korean fauna, the following three species are unknown: Lymantria xylina Swinhoe (Lymantriidae), Glyphodes actorionalis Walker (Crambidae), and Histia flabellicornis nigrinus Jordan (Zygaenidae). Interestingly, H. flabellicornis nigrinus Jordan was detected for two consecutive years. Although this species is not an important pest, it will be need to necessarily continual monitoring. L. xylina Swinhoe which is a great potential forest pest, was detected four times (16 specimens) at Busan port of entry. Therefore, it is necessary to provide comprehensive border surveillance monitoring and a valuable historical record of the array of lepidopteran hitchhikers transported to the Korea through inanimate pathway, in order to counter further potential importance of hitchhikers on international vessels at Korea.

Study of the Primo-Vascular System and Location-Dependent Oxygen Levels for a Mouse Embryo

Hong, Minyoung,Park, Sarah S.,Do, Hyunkyung,Jhon, Gil-Ja,Suh, Minah,Lee, Youngmi American Scientific Publishers 2012 Journal of Nanoscience and Nanotechnology Vol.12 No.7

<P>The two major circulatory systems, the lymph system and the blood vessel system, play significant roles in controlling embryonic development. The primo-vascular system (PVS) was recently reported as an additional circulatory system in various animals. In this paper, the PVS in a mouse embryo was investigated. The structural characterization of the PVS in the mouse placenta and umbilical cord, which was visualized with the trypan blue staining technique, was focused on. The PVS was well_developed in the mouse placenta area. Using a nanopore-based amperometric oxygen sensor, the oxygen levels at four different areas of the embryonic brain, placenta, blood vessel, and primo-vessel of the PVS were measured. The relatively higher oxygen levels that were measured at the primo-vessels than at the brain and the placenta, while still lower than the oxygen level that was measured at the blood vessels, may suggest a role of PVS in oxygen transport.</P>

The Occurrence of Subtropical-moths through the Inanimate Pathway

Minyoung Kim,Young-Mi Park,Ki-Jeong Hong,Young-Ju Park,Heung-Sik Lee 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.10

I) Surveillance for subtropical-moths through the inanimate pathway in the Korean Peninsula was investigated during the period from 2006 to the present using the light trap in Is. Baekryeong-do and Is. Daecheong-do. A total of 438 species belonging to 27 families of Lepidoptera were identified. Among them, two species of subtropical -moths, including Ischyja manlia Cramer and Spirama sp. belong to the family Noctuidae were occurred at Is. Daecheong-do in the west coast. II) We are conducting an investigation into subtropical-moths detected from a vessel arrived in Yeosu sea port from this year. Among them, one species was identified into Histia flabellicornis Fabricius belong to the family Zygaenidae. These moths are mainly distributed in the subtropical area from the southern part of China to Indonesia. It is very likely that subtropical-moths have invaded in the Korean Peninsula through the inanimate pathway such as typhoons and/or vessels. To resolve this question, further researches should be done to determine the pathway by which these subtropical-moths occurred in Korea.

Effect on the herbal formulation KH against hemopoietic side effects of 5-FU in mice

Sung HyunJea,Yoon YooSik,Hong MinYoung 대한약학회 2002 大韓藥學會 總會 및 學術大會 Vol.2002 No.1

Heterogeneity of Skin Surface Oxygen Level of Wrist in Relation to Acupuncture Point

Hong, Minyoung,Park, Sarah S.,Ha, Yejin,Lee, Jaegeun,Yoo, Kwangsik,Jhon, Gil-Ja,Suh, Minah,Lee, Youngmi Hindawi Publishing Corporation 2012 Evidence-based Complementary and Alternative Medic Vol.2012 No.-

<P>The distribution of partial oxygen pressure (pO<SUB>2</SUB>) is analyzed for the anterior aspect of the left wrist with an amperometric oxygen microsensor composed of a small planar Pt disk-sensing area (diameter = 25 <I><I>μ</I></I>m). The pO<SUB>2</SUB> levels vary depending on the measurement location over the wrist skin, and they are systematically monitored in the analysis for both one-dimensional single line (along the wrist transverse crease) and two-dimensional square area of the wrist region. Relatively higher pO<SUB>2</SUB> values are observed at certain area in close proximity to the position of acupuncture points with statistical significance, indicating strong relationship between oxygen and acupuncture point. The used oxygen microsensor is sensitive enough to detect the pO<SUB>2</SUB> variation depending on the location. This study may provide information helpful to understand possible physiological roles of the acupuncture points.</P>

탄소배출량 저감을 위한 가공 철근의 로지스틱스 전략 수립에 대한 예비적 고찰 연구

홍민영(Hong, MinYoung),이준성(Yi, June-Seong) 대한건축학회 2018 대한건축학회 학술발표대회 논문집 Vol.38 No.2

Hot Topics : P1: Transcriptome Changes for Psoriasis Vulgaris and Atopic Dermatitis in Skin Barrier Homeostasis

( Minyoung Jung ),( Chan Hee Nam ),( Byung Cheol Park ),( Myung Hwa Kim ),( Seung Phil Hong ) 한국피부장벽학회 2015 한국피부장벽학회지 Vol.17 No.2

Atopic dermatitis (AD) and psoriasis vulgaris (PSO) are most representative inflammatory skin disease. Skin barrier dysfunction is commonly seen in AD and PSO. However, their clinical features are quite different due to the pathogenetic difference. To deeply understand the clinical as well as pathogenetic differences, we have analyzed the genes involved in the epidermal lipid synthesis, epidermal development process and immune responses in the skin of AD and PSO patients. We have compared the transcriptomes of lesional skin from 6 adults AD patients (males), 4 PSO patients (1 female and 3 males) and 6 healthy young volunteers (19~24 years of age, 3 females and 3 males) by high-throughput complementary DNA sequencing (RNA-seq). For the gene expression estimation, Cufflinks v2.1.1 was used that is the gene annotation database of Ensembl release 72. 3,121 and 6,669 differentially expressed genes (DEGs) were identified in the skin of AD and PSO patients, respectively, compared with healthy skin (p<0.05). Comparing two disease groups, an overlap containing 397 genes was detected. The criterion for inclusion in the list was at least two-fold under- or over- expression in the same direction in both diseases. These transcriptomes contained AD- and PSO-associated genes for confirming diseases such as carbonic anhydrase Ⅱ (CAⅡ), neural epidermal growth factor Ⅱ (NELLⅡ), thymus and activation-regulated chemokine (TARC/CCL17), β-defensin-2 (DEFB4), psoriasin (S100A7), elafin (PI3), IL-17A, Ki67, and IL-12. The DEGs of AD and PSO groups were clustered into the GOs related to lipids metabolism, epidermal differentiation and development process, and immune response. This data help us understand the gene expressional differences in the skin barrier homeostasis pathway, cytoskeletal changes, and inflammatory response.