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( R. Anandham ),( P. Indiragandhi ),( M. Madhaiyan ),( Jong Bae Chung ),( Kyoung Yul Ryu ),( Hyeong Jin Jee ),( Tong Min Sa ) 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.1
The mixotrophic growth with methanol plus thiosulfate was examined in nutrient-limited mixotrophic condition for Methylobacterium goesingense CBMB5 and Methylobacterium fujisawaense CBMB37. Thiosulfate oxidation increased the growth and protein yield in mixotrophic medium that contained 150mM methanol and 20mM sodium thiosulfate, at 144 h. Respirometric study revealed that thiosulfate was the most preferable reduced inorganic sulfur source, followed by sulfite and sulfur. M. goesingense CBMB5 and M. fujisawaense CBMB37 oxidized thiosulfate directly to sulfate, and intermediate products of thiosulfate oxidation such as polythionates, sulfite, and sulfur were not detected in spent medium and they did not yield positive amplification for tested soxB primers. Enzymes of thiosulfate oxidation such as rhodanese and sulfite oxidase activities were detected in cell-free extracts of M. goesingense CBMB5, and M. fujisawaense CBMB37, and thiosulfate oxidase (tetrathionate synthase) activity was not observed. It indicated that both the organisms use the "non-S4 intermediate" sulfur oxidation pathway for thiosulfate oxidation. It is concluded from this study that M. goesingense CBMB5, and M. fujisawaense CBMB37 exhibited mixotrophic metabolism in medium containing methanol plus thiosulfate and that thiosulfate oxidation and the presence of a "Paracoccus sulfur oxidation" (PSO) pathway in methylotrophic bacteria are species dependant.
Madhaiyan, M,Poonguzhali, S,Senthilkumar, M,Pragatheswari, D,Lee, K-C,Lee, J-S N.V. Swets en Zeitlinger 2013 Antonie van Leeuwenhoek Vol.103 No.3
<P>A novel plant-associated obligate methylotrophic bacterium, designated strain Ca-68(T), was isolated from the rhizosphere soil of field-grown red pepper from India. The isolates are strictly aerobic, Gram negative, motile rods multiplying by binary fission and formaldehyde is assimilated via the ribulose monophosphate pathway. A comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacillus flagellatus, Methylobacillus glycogens and Methylobacillus pratensis, with which it showed pairwise similarity of 97.8, 97.4 and 96.2 %, respectively. The major fatty acids are C(16:0), C(10:0) 3OH and C(16:1) ω7c. The G+C content of the genomic DNA is 59.7 mol%. The major ubiquinone is Q-8. Dominant phospholipids are phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Based on 16S rRNA gene sequence analysis and DNA-DNA relatedness (14-19 %) with type strains of the genus Methylobacillus, the novel isolate was classified as a new species of this genus and named Methylobacillus rhizosphaerae Ca-68(T) (=KCTC 22383(T) = NCIMB 14472(T)).</P>
Madhaiyan, M.,Poonguzhali, S.,Saravanan, V. S.,Hari, K.,Lee, K.-C.,Lee, J.-S. International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.3
<P>Two strains, designated Sac-22<SUP>T</SUP> and Sac-41<SUP>T</SUP>, were isolated from rhizosphere soil and rhizoplane of field-grown sugar cane clone Co86032. Comparative 16S rRNA gene sequence analysis showed a clear affiliation of these two bacteria with the class <I>Betaproteobacteria</I>, their closest relatives being <I>Pseudoduganella violaceinigra</I> and <I>Duganella zoogloeoides</I> with 16S rRNA gene sequence pairwise similarities of 96.4–97.2 % to the two novel strains. Strains Sac-22<SUP>T</SUP> and Sac-41<SUP>T</SUP> shared a 16S rRNA gene sequence similarity value of 97.6 %. Cells of the two strains were Gram-reaction-negative, aerobic, motile and rod-shaped. Ubiquinone (Q-8) was the respiratory quinone and the predominant polar lipids consisted of phosphatidylglycerol and phosphatidylethanolamine. The main cellular fatty acids were C<SUB>16 : 0</SUB>, C<SUB>16 : 1</SUB>ω7<I>c</I>/iso-C<SUB>15 : 0</SUB> 2-OH, C<SUB>17 : 0</SUB> cyclo, C<SUB>10 : 0</SUB> 3-OH and C<SUB>12 : 0</SUB>. The DNA G<I>+</I>C content of the genomic DNA was 56.4 mol% for strain Sac-22<SUP>T</SUP> and 54.9 mol% for strain Sac-41<SUP>T</SUP>. Based on the results of 16S rRNA gene sequence analysis and physiological and biochemical characterization, that differentiated strains Sac-22<SUP>T</SUP> and Sac-41<SUP>T</SUP> from all recognized species of the genus <I>Duganella</I>, it was concluded that strains represent two novel species in the genus <I>Duganella</I> for which the names <I>Duganella</I> <I>sacchari</I> sp. nov. (type strain Sac-22<SUP>T</SUP> = KCTC 22381<SUP>T</SUP> = NCIMB 14475<SUP>T</SUP>) and <I>Duganella</I> <I>radicis</I> sp. nov. (type strain Sac-41<SUP>T</SUP> = KCTC 22382<SUP>T</SUP> = NCIMB 14476<SUP>T</SUP>) are proposed.</P>