http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 사동민 ( Tong Min Sa ) (검색결과 169 건)
- 무료
- 기관 내 무료
- 유료
-
사동민,Sa, Tong-Min,Israel, Daniel W. 한국응용생명화학회 1991 Applied Biological Chemistry Vol.34 No.2
기주 식물에 대한 인산공급이 근류의 인산 형태와 bacteroid의 인산흡수에 미치는 영향을 살펴보고자 B. japonicum MN 110을 접종한 대두식물을 1.0, 0.25, 0.05 mM-P 조건에서 재배하였다. 1.0, 0.25, 0.05 mM-P 조건에서 재배한 대두식물의 근류 Pi 농도는 각각 4.1, 2.5, 2.0 mM이었으며 인산공급이 근류의 Pi, SOP, lOP의 분포에는 유의성 있는 영향을 미치지 못하였다. 0.25, 0.05 mM-P 조건에서 재배한 대두식물의 유엽 Pi 농도는 대조구(1.0 mM-P)의 각각 33%, 20%이었으며 노엽 Pi 농도는 대조구의 각각 16%, 7%였다. 잎에서는 인산 결핍이 전인산에 대한 Pi의 분포를 감소시켰으나 lOP의 분포는 증가시켰다. 근류내의 bacteroid의 수는 $0.87-1.30{\times}10^{11}\;/GFW\;nodule$이었으며, 인산흡수도는 15-16 pmoles /min/$10^8$ bacteroid로 기주식물의 인산공급에는 영향을 받지 않았다 이러한 결과는 인산결핍 대두식물의 근류내 Pi농도는 bacteroid의 생육과 번식에 충분하며 인산결핍에 의하여 기주식물의 생육이 저해될 때에도 bacteroid의 인산흡수는 억제상태에 있음을 나타낸다. Soybean plants inoculated with Bradyrhizobium japonicum MN 110 were supplied with nutrient solutions containing 1.0, 0.25 and 0.5.nM-P to characterize the effect of externaI-P supply on the phosphorus status of nodules and on the P-uptake system of isolated bacteroids from nodules. After 48 days of growth, whole plant dry mass in the 0.25 and 0.05 mM-P treatments decreased significantly. The Pi concentrations in nodules were 4.1, 2.5 and 2.0 mM for 1.0, 0.25 and 0.05 mM-P treatments, respectively. The external-P supply did not significantly affect the distribution of phosphorus among inorganic phosphate(Pi), soluble organic-phosphorus(SOP) and insoluble organic-phosphorus(TOP) fractions in nodules. The Pi concentrations in young leaves of 0.25 and 0.05 mM-P plants were 33% and 20% , respectively, of those in young leaves of 1.0 mM-P plants and Pi concentrations in old leaves were only 16% and 7%, respectively, of those in old leaves of 1.0 mM-P plants. Phosphorus deficiency decreased the percentage of total leaf phosphorus in the Pi fraction and increased the percentage of total leaf phosphorus in the IOP fraction. The bacteroid number ranged from 0.87 to $1.30{\times}10^{11}$ Per GFW nodule regardless of external-P supply to the host Plants and Plant age, The P-uptake rates were the same (15-16 pmoles /min./$10^8$ bacteroids) for the bacteroids isolated from nodules of 1.0 mM-P and 0.05 mM-P plants. These results indicate that Pi concentrations in nodules of phosphorus-deficient plants are sufficient for proliferation of bacteroids and that the P-uptake system of bacteroids is in a repressed state even when host plant growth is severely restricted by phosphorus-deficiency stress.
-
Ureide Distribution in Nitrogen Fixing Soybean Plant under External Phosphorus Limitation
사동민,Sa, Tong-Min 한국응용생명화학회 1997 Applied Biological Chemistry Vol.40 No.3
인산 결핍이 질소고정식물체의 Ureide 농도 및 분배에 미치는 영향을 살펴보고자 Bradyrhizobium japonicum MN 110을 접종한 대두 식물체를 1.0 mM-P(대조구) 및 0.05 mM-P(결핍구)를 함유한 무질소 영양액을 처리, 재배하여 이식 28, 35,42, 49일 후 수확하였다. 3주의 실험기간중 건물량은 대조구의 경우 8.9배, 결점구의 경우 2.7배 증가하였다. 인산 결핍구에서 식물체의 전질소 및 인산의 함량은 이식 후 28일에 각각 80%,40%,49일에 각각 93%, 84% 감소하였으며 뿌리 및 근류보다 잎과 줄기의 질소 농도가 크게 감소하였다. 인산 결핍은 잎과 줄기의 수용태 및 ureide태 질소 농도를 감소시켰으나 뿌리의 경우 영향을 받지 않았으며 인산결점구의 경우 전체 수용태 및 ureide태 질소의 함량중 뿌리 함유량 비율이 대조구에 비하여 증가하였다. 이러한 결과는 인산 결핍이 근류의 질소고정뿐만 아니라 고정질소의 상향 이동도 저해함을 나타낸다. Soybean plants inoculated Bradrhizobium japonicum MN 110 were grown in outdoor perlite pots with nitrogen free nutrient solution containing 1.0 mM-P(control) and 0.05 mM-P(stress) and harvested at 28, 35, 42 and 49 days after transplanting (DAT) to examine the effect of phosphorus deficiency on ureide concentration of and distribution to diffrent plant organ in nitrogen fixing soybean plant during the vegetative growth. Total dry mass of control plants increased 8.9 fold and that of phosphorus deficient plant increased 2.7 fold during the experimental period. Phosphorus deficiency reduced total phosphorus and nitrogen accumulation by 80%,40% respectively, at 28 DAT and 93%, 84%, respectively, at 49 DAT. Nitrogen concentration was reduced by phosphorus deficiency in all tissues with leaf and stem tissues affected to a greater degree than nodule and root tissues at every sampling date. Phosphorus deficiency significantly reduced soluble reduced-N and ureide-N concentration in leaf and stem but did not affect those in root. The proportion of soluble reduced-N in leaf was reduced from 60% to 50% but increased from 10% to 20% in the roots. The proportion of ureide-N in leaf of control plants was higher than that in phosphorus deficient plants, whereas, roots of phosphorus deficient plants contained a higher propotion of ureide-N than those of control plants. These indicated that phosphorus deficiency not only inhibit nitrogen fixation of nodules but also restrict the translocation of fixed nitrogen out of the root system into the xylem.(Received April 4, 1997; accepted May 2, 1997)
-
The effect of phosphorus stress on the energy status and bacteroid content in soybean nodules
사동민,임선욱,Sa, Tong-Min,Lim, Sun-Uk,Israel, Daniel W. 한국응용생명화학회 1992 Applied Biological Chemistry Vol.35 No.6
인산처리가 근류의 bacteroid 함량과 energy 상태에 미치는 영향을 살펴보기 위하여 Bradyrhizobium japonicum MN10을 접종한 대두식물(Glycine max [L.] Merr.)에 저해농도(0.05 mM-P)와 정상농도(1.0 mM-P)를 처리하여 온실에서 재배하였다. 인산결핍은 식물체의 근류량과 근류량 식물체량의 비에 유의성있는 감소를 보였다. 잎, 줄기, 뿌리의 인산농도는 인산결핍에 의하여 75%가 감소하였으나 근류의 인산농도는 40%만 감소하였다. 근류의 bacteroid 함량과 근류에서 전인산, 전질소의 bacteroid와 식물세포로의 분배는 인산결핍의 영향을 받지 않았으며 전질소의 22%, 전인산의 27%가 bacteroid에 존재하였다. 근류의 ATP농도, 전 adenylate 농도와 energy charge는 인산결핍에 의하여 각각 77%, 46%, 37%가 감소되었다. 인산결핍은 근류내 식물세포의 ATP 농도와 energy charge를 각각 86%, 59%를 감소시켰으나 bacteroid의 ATP농도와 energy charge에는 영향을 미치지 않았다. 이러한 결과는 근류가 인산의 강한 보유원이며, 기주식물에 대한 인산결핍조건하에서 bacteroid는 정상적인 인산 농도와 energy양을 함유한다는 것을 나타낸다. Expermient were conducted to determine the effect of phosphorus stress on bacteroid content and energy status of soybean (Glycine max [L.] Merr.) nodules. Plants inoculated with Bradyrhizobium japonicum MN 110 were grown with P-stressed (0.05 mM-P) and control (1 mM-P) treatment in the greenhouse. Phosphorus stress decreased nodule mass per plant and nodule mass to whole plant mass ratio. Phosphorus concentration in leaf, stem and root tissues were reduced by 75% but in nodule tissue was reduced only by 40% under phosphorus stress during 3 week experimental period. The bacteroid content per unit nodule mass and the distribution of total nitrogen and total phosphorus among the bacteroid and plant cell fractions of nodule were not affected significantly by phosphorus stress. Regardless of phosphorus treatment, 22% of the nitrogen and 27% of the phosphorus in whole nodules were associated with the bacteroid fraction. The ATP and total adenylate concentrations in and energy charge of whole nodule were decreased 77%, 46% and 37%, respectively, by phosphorus stress. The ATP concentration in and energy charge of the host plant cell fraction of nodules were decreased 86% and 59%, respectively, but these parametres in bacteroid in nodules were not affected by phosphorus stress. These results indicated that nodule is a strong phosphorus sink and that nodule growth and development are regulated to maintain a high phosphorus and energy content in bacteroid even when the host plant is subjected to phosphorus deficiency.
-
사동민,Sa, Tong-Min 한국응용생명화학회 1997 Applied Biological Chemistry Vol.40 No.2
대기 $CO_2$ 상승시 인산공급이 대두식물체의 광합성 및 질소고정에 미치는 영향을 살펴보고저 Bradyrhizobium japonicum MN110을 접종한 대두 식물체를 인산결핍구(0.05 m-P) 및 대조구(1.0 mM-P)를 처리하여 정상 $CO_2(400\;{\mu}l/L)$와 상승 $CO_2(800\;{\mu}l/L)$의 조건인 phytotron에서 35일 동안 재배하였다. 인산결핍은 건물량을 정상 $CO_2$ 처리시 51%, 상승 $CO_2$ 처리시 64%를 감소시켰고 총 엽면적도 유의성있게 감소시켰으나 specific leaf weight는 증가시켰다. 광합성 속도 및 잎내부의 $CO_2$농도는 인산결집구에서 감소하였고 그 정도는 $CO_2$ 상승처리시 더욱 컸으며 질소고정능과 근류생체량도 인산결핍시 감소하였으나 잎의 질소농도는 인산대조구가 30% 감소하였다. 이러한 결과는 $CO_2$ 상승조건에서도 인산이 결핍될 경우 식물생육을 촉진시키지 못하며 인산은 정상 $CO_2$ 농도시 보다 $CO_2$ 상승조건에서 근류의 성장과 작용에 더욱 중요한 역할을 하는것으로 유추된다. The objective of this study was to examine the effect of phosphorus deficiency on nitrogen fixation and photosynthesis of nitrogen fixing soybean plant under $CO_2$ enrichment condition. The soybean plants(Glycine max [L.] Merr.) inoculated with Bradyrhizobium japonicum MN 110 were grown with P-stressed(0.05 mM-P) and control(1 mM-P) treatment under control$(400\;{\mu}l/L\;CO_2)$ and enrichment$(800\;{\mu}l/L\;CO_2)$ enviromental condition in the phytotron equipped with high density lamp$(1000\;{\mu}Em^{-2}S^{-1})$ and $28/22^{\circ}C$ temperature cycle for 35 days after transplanting(DAT). At 35 DAT, phosphorus deficiency decreased total dry mass by 64% in $CO_2$ enrichment condition, and 51% in control $CO_2$ condition. Total leaf area was reduced significantly by phosphorus deficiency in control and enriched $CO_2$ condition but specific leaf weight was increased by P deficiency. Phosphorus deficiency significantly reduced photosynthetic rate(carbon exchange rate) and internal $CO_2$ concentration in leaf in both $CO_2$ treatments, but the degree of stress was more severe under $CO_2$ enrichment condition than under control $CO_2$ environmental condition. In phosphorus sufficient plants, $CO_2$ enrichment increased nodule fresh weight and total nitrogenase activity(acetylene reduction) of nodule by 30% and 41% respectively, but specific nitrogenase activity of nodule and nodule fresh weight was not affected by $CO_2$ enrichment in phosphorus deficient plant at 35 DAT. Total nitrogen concentrations in stem, root and nodule tissue were significantly higher in phosphorus sufficient plant grown under $CO_2$ enrichment, but nitrogen concentration in leaf was reduced by 30% under $CO_2$ enrichment. These results indicate that increasing $CO_2$ concentration does not affect plant growth under phosphorus deficient condition and phosphorus stress might inhibit carbohydrate utilization in whole plant and that $CO_2$ enrichment could not increase nodule formation and functioning under phosphorus deficient conditions and phosphorus has more important roles in nodule growth and functioning under $CO_2$ enrichment environments than under ambient condition.
-
-
-
-
-
-
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
