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Anandham, R.,Sridar, R.,Nalayini, P.,Poonguzhali, S.,Madhaiyan, M.,sa, T. G. Fischer 2007 MICROBIOLOGICAL RESEARCH Vol.162 No.2
The use of Rhizobium inoculant for groundnut is a common practice in India. Also, co-inoculation of Rhizobium with other plant growth-promoting bacteria received considerable attention in legume growth promotion. Hence, in the present study we investigated effects of co-inoculating the sulfur (S)-oxidizing bacterial strains with Rhizobium, a strain that had no S-oxidizing potential in groundnut. Chemolithotrophic S-oxidizing bacterial isolates from different sources by enrichment isolation technique included three autotrophic (LCH, SWA5 and SWA4) and one heterotrophic (SGA6) strains. All the four isolates decreased the pH of the growth medium through oxidation of elemental S to sulfuric acid. Characterization revealed that these isolates tentatively placed into the genus Thiobacillus. Clay-based pellet formulation (2.5x10<SUP>7</SUP>cfug<SUP>-1</SUP> pellet) of the Thiobacillus strains were developed and their efficiency to promote plant growth was tested in groundnut under pot culture and field conditions with S-deficit soil. Experiments in pot culture yielded promising results on groundnut increasing the plant biomass, nodule number and dry weight, and pod yield. Co-inoculation of Thiobacillus sp. strain LCH (applied at 60kgha<SUP>-1</SUP>) with Rhizobium under field condition recorded significantly higher nodule number, nodule dry weight and plant biomass 136.9 plant<SUP>-1</SUP>, 740.0mg plant<SUP>-1</SUP> and 15.0g plant<SUP>-1</SUP>, respectively, on 80 days after sowing and enhanced the pod yield by 18%. Also inoculation of S-oxidizing bacteria increased the soil available S from 7.4 to 8.43kgha<SUP>-1</SUP>. These results suggest that inoculation of S-oxidizing bacteria along with rhizobia results in synergistic interactions promoting the yield and oil content of groundnut, in S-deficit soils.
A. Poonguzhali,S. Ningshen,G. Amarendra 대한금속·재료학회 2020 METALS AND MATERIALS International Vol.26 No.10
Corrosion fatigue behavior of type 316N stainless steel (SS) weldments on the damage characteristics is studied. The S–Ncurve of the fatigue life versus stress amplitude showed a specific two slope Basquin relationship. At low stress amplitude,the deformation microstructure consists mainly of planar slip dislocation structure and at high-stress amplitudes, planarmultiple slip dislocations govern the cyclic behavior. A correlation between microstructural changes and the correspondingsusceptibility of 316N SS weldment in as-welded condition towards environmental cracking in boiling acidified aqueoussolution has been presented. The objective of the present study is to investigate the environmental effects on the corrosionfatigue crack initiation of type 316N weldmetal under the influence of cyclic stress amplitude. The difference in deformationmicro mechanisms with stress amplitude in a corrosive environment is believed to be the major reason for the occurrence ofa specific bilinear Basquin relationship in the S–N curve.
Madhaiyan, M.,Poonguzhali, S.,Saravanan, V. S.,Hari, K.,Lee, K.-C.,Lee, J.-S. International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.3
<P>Two strains, designated Sac-22<SUP>T</SUP> and Sac-41<SUP>T</SUP>, were isolated from rhizosphere soil and rhizoplane of field-grown sugar cane clone Co86032. Comparative 16S rRNA gene sequence analysis showed a clear affiliation of these two bacteria with the class <I>Betaproteobacteria</I>, their closest relatives being <I>Pseudoduganella violaceinigra</I> and <I>Duganella zoogloeoides</I> with 16S rRNA gene sequence pairwise similarities of 96.4–97.2 % to the two novel strains. Strains Sac-22<SUP>T</SUP> and Sac-41<SUP>T</SUP> shared a 16S rRNA gene sequence similarity value of 97.6 %. Cells of the two strains were Gram-reaction-negative, aerobic, motile and rod-shaped. Ubiquinone (Q-8) was the respiratory quinone and the predominant polar lipids consisted of phosphatidylglycerol and phosphatidylethanolamine. The main cellular fatty acids were C<SUB>16 : 0</SUB>, C<SUB>16 : 1</SUB>ω7<I>c</I>/iso-C<SUB>15 : 0</SUB> 2-OH, C<SUB>17 : 0</SUB> cyclo, C<SUB>10 : 0</SUB> 3-OH and C<SUB>12 : 0</SUB>. The DNA G<I>+</I>C content of the genomic DNA was 56.4 mol% for strain Sac-22<SUP>T</SUP> and 54.9 mol% for strain Sac-41<SUP>T</SUP>. Based on the results of 16S rRNA gene sequence analysis and physiological and biochemical characterization, that differentiated strains Sac-22<SUP>T</SUP> and Sac-41<SUP>T</SUP> from all recognized species of the genus <I>Duganella</I>, it was concluded that strains represent two novel species in the genus <I>Duganella</I> for which the names <I>Duganella</I> <I>sacchari</I> sp. nov. (type strain Sac-22<SUP>T</SUP> = KCTC 22381<SUP>T</SUP> = NCIMB 14475<SUP>T</SUP>) and <I>Duganella</I> <I>radicis</I> sp. nov. (type strain Sac-41<SUP>T</SUP> = KCTC 22382<SUP>T</SUP> = NCIMB 14476<SUP>T</SUP>) are proposed.</P>
Design and performance analysis of smart photonic sensors for industrial applications
S Poonguzhali,Sivasangari A.,Ajitha P.,Lalithakumari S.,Sridevi A.,Danasegaran Sathish Kumar 한국물리학회 2022 Current Applied Physics Vol.39 No.-
Toxic gas has a median fatal concentration in the oxygen of much more than 200 parts per million (ppm) but far less than 2000 ppm by volume of gas. Many industries, mines and thermal plants emit perilous gases that are more harmful to our human life. The Proposed nanosensor senses the various perilous gases and averts many accidents. In this paper, a two-dimensional Photonic Crystal (2D-PhC) resonator and PhC-based poisonous gas sensor based on the hexagonal and square crystal lattice are built-in smart way. The PhCs are artificial constructs of any material with an occasional enunciation of refractive index (RI). It has effective light manipulation and it would be helpful to obtain light migration in the handling of sensing applications. The TE/TM wave transmission can shift as per the RI value of different gases in the PhCs. The wavelength variations obtained agree well with the Finite Difference Time Domain (FDTD) study, and the simulation is performed by the tool RSoft. The spectral variables such as quality factor (QF), sensitivity (Se), transmitted output power and detection limit (DL) are evaluated using the RI value over the spectrum of different toxic gases. The proposed square crystal structure have acquired a QF range of 500.6, high efficiency of 99%, and a better Se of 716.6 nm/RIU at 1502 nm. The designed hexagonal crystal structure have acquired a QF range of 165.8, high efficiency of 99%, and a better Se of 798.24 nm/RIU at 1630 nm respectively. The DL for both the proposed sensors is very low. So, the designed smart sensor helps promptly recognize the contaminated gases in several places. The proposed nanosensor is helpful in industrial safety, health care applications, aerospace, agricultural, transportation, environmental monitoring, thermal plants and mines.
Pachai Poonguzhali(Pachai Poonguzhali ),Ashish Chauhan(Ashish Chauhan ),Abinash Kar(Abinash Kar ),Shivaji Lavale(Shivaji Lavale ),Spurthi N. Nayak(Spurthi N. Nayak ),S. K. Prashanthi(S. K. Prashanthi 한국식물병리학회 2022 Plant Pathology Journal Vol.38 No.6
Sheath blight disease caused by the necrotrophic, soilborne pathogen Rhizoctonia solani Kuhn, is the global threat to rice production. Lack of reliable stable resistance sources in rice germplasm pool for sheath blight has made resistance breeding a very difficult task. In the current study, 101 rice landraces were screened against R. solani under artificial epiphytotics and identified six moderately resistant landraces, Jigguvaratiga, Honasu, Jeer Sali, Jeeraga-2, BiliKagga, and Medini Sannabatta with relative lesion height (RLH) range of 21-30%. Landrace Jigguvaratiga with consistent and better level of resistance (21% RLH) than resistant check Tetep (RLH 28%) was used to develop mapping population. DNA markers associated with ShB resistance were identified in F2 mapping population developed from Jigguvaratiga × BPT5204 (susceptible variety) using bulk segregant analysis. Among 56 parental polymorphic markers, RM5556, RM6208, and RM7 were polymorphic between the bulks. Single marker analysis indicated the significant association of ShB with RM5556 and RM6208 with phenotypic variance (R2) of 28.29 and 20.06%, respectively. Co-segregation analysis confirmed the strong association of RM5556 and RM6208 located on chromosome 8 for ShB trait. This is the first report on association of RM6208 marker for ShB resistance. In silico analysis revealed that RM6208 loci resides the stearoyl ACP desaturases protein, which is involved in defense mechanism against plant pathogens. RM5556 loci resides a protein, with unknown function. The putative candidate genes or quantitative trait locus harbouring at the marker interval of RM5556 and RM6208 can be further used to develop ShB resistant varieties using molecular breeding approaches.
Madhaiyan, M,Poonguzhali, S,Senthilkumar, M,Pragatheswari, D,Lee, K-C,Lee, J-S N.V. Swets en Zeitlinger 2013 Antonie van Leeuwenhoek Vol.103 No.3
<P>A novel plant-associated obligate methylotrophic bacterium, designated strain Ca-68(T), was isolated from the rhizosphere soil of field-grown red pepper from India. The isolates are strictly aerobic, Gram negative, motile rods multiplying by binary fission and formaldehyde is assimilated via the ribulose monophosphate pathway. A comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacillus flagellatus, Methylobacillus glycogens and Methylobacillus pratensis, with which it showed pairwise similarity of 97.8, 97.4 and 96.2 %, respectively. The major fatty acids are C(16:0), C(10:0) 3OH and C(16:1) ω7c. The G+C content of the genomic DNA is 59.7 mol%. The major ubiquinone is Q-8. Dominant phospholipids are phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Based on 16S rRNA gene sequence analysis and DNA-DNA relatedness (14-19 %) with type strains of the genus Methylobacillus, the novel isolate was classified as a new species of this genus and named Methylobacillus rhizosphaerae Ca-68(T) (=KCTC 22383(T) = NCIMB 14472(T)).</P>