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( Kunbawui Park ),( Jong Soo Mok ),( Ji Young Kwon ),( A Ra Ryu ),( Song Hee Kim ),( Hee Jung Lee ) 한국수산과학회(구 한국수산학회) 2018 Fisheries and Aquatic Sciences Vol.21 No.1
Background: Vibrio parahaemolyticus is one of the most common causes of seafood-borne illnesses in Korea, either directly or indirectly, by consuming infected seafood. Many studies have demonstrated the antibiotic susceptibility profile of V. parahaemolyticus. This strain has developed multiple antibiotic resistance, which has raised serious public health and economic concerns. This article reviews the food-borne outbreaks, distributions, virulence, and antibiotic resistance profiles of V. parahaemolyticus in Korea during 2003-2016. Main body: V. parahaemolyticus infections appeared to be seasonally dependent, because 69.7% of patient infections occurred in both August and September during 2003-2016. In addition, the occurrence of V. parahaemolyticus in marine environments varies seasonally but is particularly high in July, August, and September. V. parahaemolyticus isolated from aquaculture sources on the Korean coast varied in association with virulence genes, some did not possess either the tdh (thermostable direct hemolysin) or trh (tdh-related hemolysin) genes, and a few were positive for only the trh gene or both genes. The high percentage of ampicillin resistance against V. parahaemolyticus in the aquatic environment suggests that ampicillin cannot be used to effectively treat infections caused by this organism. Short conclusion: This study shows that the observed high percentage of multiple antibiotic resistance to V. parahaemolyticus is due to conventionally used antibiotics. Therefore, monitoring the antimicrobial resistance patterns at a national level and other solutions are needed to control aquaculture infections, ensure seafood safety, and avoid threats to public health caused by massive misuse of antibiotics.
Park, Kunbawui,Mok, Jong Soo,Kwon, Ji Young,Ryu, A Ra,Kim, Song Hee,Lee, Hee Jung The Korean Society of Fisheries and Aquatic Scienc 2018 Fisheries and Aquatic Sciences Vol.21 No.2
Background: Vibrio parahaemolyticus is one of the most common causes of seafood-borne illnesses in Korea, either directly or indirectly, by consuming infected seafood. Many studies have demonstrated the antibiotic susceptibility profile of V. parahaemolyticus. This strain has developed multiple antibiotic resistance, which has raised serious public health and economic concerns. This article reviews the food-borne outbreaks, distributions, virulence, and antibiotic resistance profiles of V. parahaemolyticus in Korea during 2003-2016. Main body: V. parahaemolyticus infections appeared to be seasonally dependent, because 69.7% of patient infections occurred in both August and September during 2003-2016. In addition, the occurrence of V. parahaemolyticus in marine environments varies seasonally but is particularly high in July, August, and September. V. parahaemolyticus isolated from aquaculture sources on the Korean coast varied in association with virulence genes, some did not possess either the tdh (thermostable direct hemolysin) or trh (tdh-related hemolysin) genes, and a few were positive for only the trh gene or both genes. The high percentage of ampicillin resistance against V. parahaemolyticus in the aquatic environment suggests that ampicillin cannot be used to effectively treat infections caused by this organism. Short conclusion: This study shows that the observed high percentage of multiple antibiotic resistance to V. parahaemolyticus is due to conventionally used antibiotics. Therefore, monitoring the antimicrobial resistance patterns at a national level and other solutions are needed to control aquaculture infections, ensure seafood safety, and avoid threats to public health caused by massive misuse of antibiotics.
Park, Kunbawui,Mok, Jong Soo,Ryu, A Ra,Kwon, Ji Young,Ham, In Tae,Shim, Kil Bo Elsevier 2018 MARINE POLLUTION BULLETIN Vol.137 No.-
<P><B>Abstract</B></P> <P>We determined the abundance and virulence of <I>Vibrio parahaemolyticus</I> in seawater and bivalves from the Gyeongnam coast in Korea, a major area for the seafood industry, during 2004–2016. <I>V. parahaemolyticus</I> is one of the most common pathogen causing seafood-borne illnesses in Korea, and increases during the summer. Its occurrence in seawater and bivalve samples was seasonally dependent, with high levels during the summer to early autumn. There were more strains in the area of sea continually exposed to inland wastewater. Only 5.1% and 3.5% of <I>V. parahaemolyticus</I> isolates from seawater and bivalves, respectively, had the <I>trh</I> gene, and only the bivalve isolates produced the <I>tdh</I> gene at levels below 2%. Continuous monitoring is clearly needed to reduce seafood-borne outbreaks of disease caused by <I>V. parahaemolyticus</I>, and to reveal the occurrence patterns and the presence of toxic genes of the strains in different marine environments.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Abundance & virulence of <I>Vibrio parahaemolyticus</I> in seawater & bivalves in Korea </LI> <LI> Occurrence is seasonally dependent: high levels in summer to early autumn. </LI> <LI> More strains in sea continually exposed to inland wastewater </LI> <LI> <6% of isolates from seawater & bivalves had the toxic <I>tdh</I> or <I>trh</I> genes. </LI> </UL> </P>
한산거제만 및 자란만사량도 해역 육상오염원 배출수에서 분리한 대장균의 항균제 내성 패턴
박큰바위 ( Kunbawui Park ),김송희 ( Song Hee Kim ),함인태 ( In Tae Ham ),류아라 ( A Ra Ryu ),권지영 ( Ji Young Kwon ),김지회 ( Ji Hoe Kim ),유홍식 ( Hong Sik Yu ),이희정 ( Hee Jung Lee ),목종수 ( Jong Soo Mok ) 한국수산과학회(구 한국수산학회) 2018 한국수산과학회지 Vol.51 No.1
We investigated patterns of antimicrobial resistance in Escherichia coli isolated from the water discharged from inland pollution sources in the Hansan-Geojeman and Jaranman-Saryangdo areas of Korea. A total of 217 strains of E. coli were isolated from 23 point-sources. These strains were tested for their susceptibility to 16 antimicrobial agents used in Korea for medical or veterinary therapy. The highest level of antibiotic resistance among the isolated strains was to tetracycline 10.6%, followed by ampicillin (3.2%), nalidixic acid (2.8%), rifampin (1.8%), trimethoprim (1.8%), trimethoprim/sulfamethoxazole (1.8%), chloramphenicol (1.4%), streptomycin (1.4%), cephalothin (0.5%) and gentamicin (0.5%). Resistance to at least one antimicrobial agent was present in 17.1% of the E. coli isolates. Only four of the isolated strains of E. coli showed multiple antibiotic resistance, which is defined as resistance to more than four antibiotics.
남해안 패류양식장에서 분리된 대장균(Escherichia coli)의 항균제 내성
박큰바위 ( Kunbawui Park ),박준용 ( Jun Yong Park ),조미라 ( Mi Ra Jo ),이희정 ( Hee Jung Lee ),김지회 ( Ji Hoe Kim ),오은경 ( Eun Gyong Oh ),신순범 ( Soon Bum Shin ),김연계 ( Yeon Kye Kim ),이태식 ( Tae Seek Lee ) 한국수산과학회 2013 한국수산과학회지 Vol.46 No.5
This study evaluated the impact of antimicrobial resistant bacteria produced by inland pollution sources on coastal areas, and investigates antimicrobial resistance profiles of bacteria isolated from marine filter feeders. A total of 107shellfish (short-necked clams Ruditapes philippinarum, ark shells Scapharca broughtonii, and oysters Crassostrea gigas) were collected from the southern coast of Korea, from which 204 Escherichia coli strains were isolated. The antimicrobial susceptibility patterns of isolated strains were analyzed for 15 antimicrobial agents used in Korea for clinical or veterinary therapy. Antimicrobial resistance was present in 44.6% of E. coli isolates against at least one antimicrobial agent. The rate of antimicrobial resistance in the Narodo area was higher than isolates from the Gangjinman area and Kamak Bay. E. coli isolates had a higher rate of resistance against: tetracycline (29.9%), streptomycin (25.5%), and trimethoprim (14.2%). Of 204 isolates, 29 (14.2%) were resistant to multiple antimicrobial agents.
강우 발생에 따른 육상오염원이 나로도 해역의 세균학적 수질에 미치는 영향 평가
박큰바위 ( Kunbawui Park ),조미라 ( Mi Ra Jo ),김연계 ( Yeon Kye Kim ),이희정 ( Hee Jung Lee ),권지영 ( Ji Young Kwon ),손광태 ( Kwang Tae Son ),이태식 ( Tae Seek Lee ) 한국수산과학회(구 한국수산학회) 2012 한국수산과학회지 Vol.45 No.5
This study evaluated the effect of the inland pollution sources on the bacteriological water quality in Narodo area after rainfall events. Following 16 mm of rainfall, the water discharged from Dohwa Stream and contaminants from Balpo village had a very limited effect on bacteriological water quality in adjacent area and the affected area did not extend to the designated area. In comparion, after 33 mm of rainfall, the density of fecal coliforms at stations located in the mouth of Dohwa stream and the discharg point of contamination sources located in Balpo village were lower than after 16 mm of rainfall. These results were obtained during an extreme high tide near the coast the contaminants discharged from Dohwa stream and Balpo village. Therefore, it is necessary to conduct a detailed survey to examine the dffects of tides on the diffusion characteristics of the contaminants discharged from Dohwa stream and Balpo village in order to manage of Narodo area efficiently.
( Gyungcheon Kim ),( Gwoncheol Park ),( Seohyun Kang ),( Sanghee Lee ),( Jiyoung Park ),( Jina Ha ),( Kunbawui Park ),( Minseok Kang ),( Min Cho ),( Hakdong Shin ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.12
Outbreaks of food poisoning due to the consumption of norovirus-contaminated shellfish continue to occur. Male-specific (F+) coliphage has been suggested as an indicator of viral species due to the association with animal and human wastes. Here, we compared two methods, the double agar overlay and the quantitative real-time PCR (RT-PCR)-based method, for evaluating the applicability of F+ coliphage-based detection technique in microbial contamination tracking of shellfish samples. The RT-PCR-based method showed 1.6-39 times higher coliphage PFU values from spiked shellfish samples, in relation to the double agar overlay method. These differences indicated that the RT-PCR-based technique can detect both intact viruses and non-particle-protected viral DNA/RNA, suggesting that the RT-PCR based method could be a more efficient tool for tracking microbial contamination in shellfish. However, the virome information on F+ coliphage-contaminated oyster samples revealed that the high specificity of the RT-PCR- based method has a limitation in microbial contamination tracking due to the genomic diversity of F+ coliphages. Further research on the development of appropriate primer sets for microbial contamination tracking is therefore necessary. This study provides preliminary insight that should be examined in the search for suitable microbial contamination tracking methods to control the sanitation of shellfish and related seawater.
남해안 패류양식장에서 분리한 Vibrio parahaemolyticus의 병원인자분포 및 항균제내성
박용수 ( Yong Soo Park ),박큰바위 ( Kunbawui Park ),권지영 ( Ji Young Kwon ),유홍식 ( Hong Sik Yu ),이희정 ( Hee Jung Lee ),김지회 ( Ji Hoe Kim ),이태식 ( Tae Seek Lee ),김풍호 ( Poong Ho Kim ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.4
From 2013 through 2015, we investigated the contamination status and antimicrobial resistance patterns of pathogenic Vibrio parahaemolyticus in commercially valuable seawater and shellfish (Oyster Crassostrea gigas, short-neck clam Venerupis philippinarum, ark shell Scapharca broughtonii and mussel Mytilus galloprovinciallis) from the southern coast of Korea. The detection rate of V. parahaemolyticus was highest in short-neck clams (23.7%), followed by ark shells (19.2%), oysters (15.9%), mussels (13.6%), and seawater (8.6%). The following percentages of PCR assays of shellfish were positive for the thermostable direct hemolysin-related hemolysin gene (trh) : oysters (12.8%), shortneck clams(11.8%), and ark shells (3.4%). Similar assays for the thermostable direct hemolysin gene (tdh) resulted in positive results for short-neck clams (5.9%) and ark shells (3.4%). Antimicrobial resistance was present in 100% of 8 tdh (+) and 2 trh (+) V. parahaemolyticus isolates challenged with ampicillin. However, all pathogenic V. parahaemolyticus were sensitive to 14 other antibiotics. To ensure the safety of shellfish consumption, the continuous monitoring of the prevalence and distribution of virulence factors of V. parahaemolyticus in shellfish farms is needed.
Chlorothalonil-Biotransformation by Glutathione S-Transferase of Escherichia coli
김영목,Kunbawui Park,Soon-Hyun Jung,Jun-Ho Choi,김원찬,Gil-Jae Joo,이인구 한국미생물학회 2004 The journal of microbiology Vol.42 No.1
It has recently been reported that one of the most important factors of yeast resistance to the fungicide chlorothalonil is the glutathione contents and the catalytic efficiency of glutathione S-transferase (GST) (Shin et al., 2003). GST is known to catalyze the conjugation of glutathione to a wide variety of xenobiotics, resulting in detoxification. In an attempt to elucidate the relation between chlorothalonil-detoxification and GST, the GST of Escherichia coli was expressed and purified. The drug-hypersensitive E. coli KAM3 cells harboring a plasmid for the overexpression of the GST gene can grow in the presence of chlorothalonil. The purified GST showed chlorothalonil-biotransformation activity in the presence of glutathione. Thus, chlorothalonil is detoxified by the mechanism of glutathione conjugation catalyzed by GST.