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Essential protocols for in vitro evaluation of nanoparticle
Hitoshi Iwahashi,Masanori Horie,Keiko Nishio,Shigehisa Endoh,Haruhisa Kato,Katsuhide Fujita,Shinichi Kinugasa,Arisa Miyauchi,Ayako Nakamura,Junko Takahashi,Etsuo Niki,Yasukazu Yoshida,Junko Nakanishi 한국환경독성학회 2010 한국독성학회 심포지움 및 학술발표회 Vol.2010 No.11
장성국,조경원,Junko Shibato,Oksoo Han,Hitoshi Iwahashi,Shigeru Tamogami,Sajad Majeed Zargar,Akihiro Kubo,Yoshinori Masuo,Ganesh Kumar Agrawal,Randeep Rakwal 한국식물학회 2009 Journal of Plant Biology Vol.52 No.3
The 12-oxo-phytodienoic acid reductase (OPR) is a key enzyme in jasmonic acid (JA) biosynthesis. Previously, we reported the presence of 13 OPR isogenes (OsOPR1-13) in rice. OsOPRs phylogenetically belong to two subgroups, OPRI and OPRII. OsOPR13 is assigned to the second subgroup, which is involved in JA biosynthesis, while the others are found in the first subgroup. Here, we systematically investigated transcript levels of OsOPRs in various tissues and against diverse environmental stresses. Each gene was differentially involved in flower maturation, showing a tissue-specific response. OsOPR1, OsOPR2, and OsOPR13 were also active in responses to wounding, a fungal elicitor (chitosan), salt, UV-C irradiation, H2O2, and ozone exposure. In the case of JA-responsive OsOPRs (OsOPR1, OsOPR2, OsOPR6, OsOPR10, and OsOPR13), co-application of JA and SA suppressed jasmonate-induced transcript levels and delayed OsOPR10 expression. We also investigated the biochemical properties of OsOPR1 and found a flavin cofactor with optimal activity at pH7.8 and values of 0.048 min−1 kcat and 8.33 μM Km for (9S,13R)- 12-oxo-phytodienoic acid. Here, we discuss the role of OsOPRs in stress responses and floral development.
Airphoto Interpretation and GIS Analyses of the Landslides by 2018 Hokkaido Iburi-Tobu Earthquake
( Yamagishi Hiromitsu ),( Junko Iwahashi ) 대한지질공학회 2019 대한지질공학회 학술발표회논문집 Vol.2019 No.2
An intensive earthquake (M6.7) happend in Hokkaido Iburi-Tobu area on September 6th, 2018. This earthquake triggered many landslides in 20km × 20km area and claiming 36 people lives. The author interpretd the ortho photographs by Geogrphical Institute Survey (GSI), Japan, and inventoried about 10,000 sites of the landslides and classified three types such as 1) Spoon type (S), 2) Planar type (P) and 3)Jisuberi type (J). The landslide area is composed of Neogene sedimnary rocks and covering Quaternary pumice and ash up to several meters thick. Therefore, the sliding landslide materials are mainly the pumice and ash, defined by P and S type. However, the southern area is characterized by deep-seated landslides defined as dip-slipping of Neogene bed rocks. And then, we were trying the GIS Analyses by ArcGIS 10.2 for the relationship between the landslide frequency and topographic and geologic factors. The topographic factors are derived from 10m _DEM downloaded from GSI and geologic ones from digital geologic map of Hokakido by Engineering Geologic Society of Hokkaido, Japan. As the results, we revealed that most of the landslides of S type and P type occurred at the slope gradients ranging from 25-35 degrees, and in places, the slope gradients steepend at maximum 5 degree, possibly by the erosion of the landsliding, and that debris of the Jissuberi type took place along the 10-20 degrees of stratum dipping of the Neogene Formations, as well as old Jisuberi.
Randeep Rakwal,Shinzo Kimura,Junko Shibato,Kumie Nojima,Yeon-Ki Kim,남백희,Nam-Soo Jwa,Satoru Endo,Kenichi Tanaka,Hitoshi Iwahashi 한국분자세포생물학회 2008 Molecules and cells Vol.25 No.2
The carbon-ion beam (CIB) generated by the heavy-ion medical accelerator in Chiba (HIMAC) was targeted to 7-day-old rice. Physiological parameters such as growth, and gene expression profiles were examined immediately after CIB irradiation. Dose-dependent growth suppression was seen three days post-irradiation (PI), and all the irradiated plants died by 15 days PI. Microarray (Agilent rice 22K) analysis of the plants immediately after irradiation (iai) revealed effects on gene expression at 270 Gy; 353 genes were up-regulated and 87 down-regulated. Exactly the same set of genes was affected at 90 Gy. Among the highly induced genes were genes involved in information storage and processing, cellular processes and signaling, and metabolism. RT-PCR analysis confirmed the microarray data.
Rakwal, Randeep,Kimura, Shinzo,Shibato, Junko,Nojima, Kumie,Kim, Yeonki,Nahm, Baekhie,Jwa, Namsoo,Endo, Satoru,Tanaka, Kenichi,Iwahashi, Hitoshi Korean Society for Molecular Biology 2008 Molecules and cells Vol.25 No.2
The carbon-ion beam (CIB) generated by the heavy-ion medical accelerator in Chiba (HIMAC) was targeted to 7-day-old rice. Physiological parameters such as growth, and gene expression profiles were examined immediately after CIB irradiation. Dose-dependent growth suppression was seen three days post-irradiation (PI), and all the irradiated plants died by 15 days PI. Microarray (Agilent rice 22K) analysis of the plants immediately after irradiation (iai) revealed effects on gene expression at 270 Gy; 353 genes were up-regulated and 87 down-regulated. Exactly the same set of genes was affected at 90 Gy. Among the highly induced genes were genes involved in information storage and processing, cellular processes and signaling, and metabolism. RT-PCR analysis confirmed the microarray data.