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Cho, Kyoungwon,Shibato, Junko,Kubo, Akihiro,Kohno, Yoshihisa,Satoh, Kouji,Kikuchi, Shoshi,Agrawal, Ganesh Kumar,Sarkar, Abhijit,Rakwal, Randeep Kluwer Academic Publishers 2013 Biotechnology letters. Vol.35 No.4
<P>The 'ozone (O3)-responsive transcriptome' behavior in the panicles and grains of rice plant was studied individually through high-throughput oligo-DNA microarray technique. O3 differentially and separately regulated 620 and 130 genes in the panicles and grains. Among the O3-responsive genes, 176 and 444 genes were up- and down-regulated in panicle compared to 24 and 106 genes in grain, respectively. Further mapping revealed that the majority of differentially expressed genes were mainly involved in signaling, hormonal, cell wall, transcription, proteolysis, and defense events. Many previously unknown O3-responsive novel genes were identified. Inventory of 745 O3-responsive genes and their mapping will expand our knowledge on novel regulatory processes in both panicles and grains of rice; and, serve as a resource towards the designing of rice crops for future high-O3world.</P>
Comparative analysis of seed transcriptomes of ambient ozone-fumigated 2 different rice cultivars
Cho, Kyoungwon,Shibato, Junko,Kubo, Akihiro,Kohno, Yoshihisa,Satoh, Kouji,Kikuchi, Shoshi,Sarkar, Abhijit,Agrawal, Ganesh Kumar,Rakwal, Randeep Informa UK (Taylor Francis) 2013 Plant signaling & behavior Vol.8 No.11
Nakajima, Nobuyoshi,Takahashi, Shinya,Tamaoki, Masanori,Kubo, Akihiro,Aono, Mitsuko,Saji, Hikaru Korean Society of Photoscience 2002 Journal of Photosciences Vol.9 No.2
We collected seeds of Solidago virgaurea plants growing at different altitudes on the Mt Hakusan area in Japan and cultivated them in a naturally-lit green house. Three-week-old seedlings were irradiated with supplemental UV-B for 12 h each day for 1 and 2 weeks. After a week of itradiation the seedlings of the population collected from the higher altitude at Oh-nanjiho (ON) had accumulated more anthocyanins than those from the lower altitude at Bettoh-deai (BD). Levels of anthocyanins in the ON seedlings were highly correlated with the dose of UV-B radiation and the correlation was also observed after 2 weeks. The growth of the third leaves was retarded by UV-B radiation in both populations. The extent of growth retardation in the third leaves was correlated with the dose of UV -B radiation in both populations. However, no significant difference in the extent of leaf area growth was observed between the ON and BD populations. The increase in plant fresh weight was extensively inhibited in the ON seedlings after 1 week of UV-B radiation. The inhibition was recovered to those in the BD population by 2 weeks irradiation. These results indicate that these populations respond differentially to supplementary UV -B radiation during the first week. Because flavonoids such as anthocyanins play an important role in protection against UV-B radiation in many plants, populations growing at higher altitude may be better able to adapt to increased global levels of UV-B radiation.
Cho, Kyoung-Won,Kim, Dea-Wook,Jung, Young-Ho,Shibato, Junko,Tamogami, Shigeru,Yonekura, Masami,Jwa, Nam-Soo,Kubo, Akihiro,Agrawal, Ganesh Kumar,Rakwal, Randeep The Korean Society of Crop Science 2007 Journal of crop science and biotechnology Vol.10 No.2
Kinetin(KN) is an inducer of rice(Oryza sativa L.) defense/stress responses, as evidenced by the induction of inducible secondary metabolite and defense/stress protein markers in leaf. We show a novel light-dependent effect of KN-triggered defense stress responses in rice leaf. Leaf segments treated with KN(100 ${\mu}M$) show hypersensitive-like necrotic lesion formation only under continuous light illumination. Potent accumulation of two phytoalexins, sakuranetin and momilactone A(MoA) by KN that peaks at 48 h after treatment under continuous light is completely suppressed by incubation under continuous dark. Using two-dimensional gel electrophoresis we identified KN-induced changes in ribulose-1, 5-bisphosphate carboxylase/oxygenase, energy- and pathogenesis-related proteins(OsPR class 5 and 10 members) by N-terminal amino acid sequencing and mass spectrometry. These changes were light-inducible and could not be observed in the dark(and control). Present results provide a new dimension(light modulation/regulation) to our finding that KN has a potential role in the rice plant self-defense mechanism.
장성국,조경원,Junko Shibato,Oksoo Han,Hitoshi Iwahashi,Shigeru Tamogami,Sajad Majeed Zargar,Akihiro Kubo,Yoshinori Masuo,Ganesh Kumar Agrawal,Randeep Rakwal 한국식물학회 2009 Journal of Plant Biology Vol.52 No.3
The 12-oxo-phytodienoic acid reductase (OPR) is a key enzyme in jasmonic acid (JA) biosynthesis. Previously, we reported the presence of 13 OPR isogenes (OsOPR1-13) in rice. OsOPRs phylogenetically belong to two subgroups, OPRI and OPRII. OsOPR13 is assigned to the second subgroup, which is involved in JA biosynthesis, while the others are found in the first subgroup. Here, we systematically investigated transcript levels of OsOPRs in various tissues and against diverse environmental stresses. Each gene was differentially involved in flower maturation, showing a tissue-specific response. OsOPR1, OsOPR2, and OsOPR13 were also active in responses to wounding, a fungal elicitor (chitosan), salt, UV-C irradiation, H2O2, and ozone exposure. In the case of JA-responsive OsOPRs (OsOPR1, OsOPR2, OsOPR6, OsOPR10, and OsOPR13), co-application of JA and SA suppressed jasmonate-induced transcript levels and delayed OsOPR10 expression. We also investigated the biochemical properties of OsOPR1 and found a flavin cofactor with optimal activity at pH7.8 and values of 0.048 min−1 kcat and 8.33 μM Km for (9S,13R)- 12-oxo-phytodienoic acid. Here, we discuss the role of OsOPRs in stress responses and floral development.