에어볼 발파공법의 현장적용 사례연구

강대우,이신,김상욱 東亞大學校 建設技術硏究所 2005 硏究論文集 Vol.28 No.2

발파로 인한 환경적 문제는 주로 진동과 폭음에 대한 영향이나, 발파로 인한 파쇄도 및 경제성에 관해서도 효율적인 발파공법들이 많이 개발되고 있다. 이러한 문제를 해결하기 위한 방법으로 선진국에서 많이 사용되는 방법이 air decking을 이용하는 발파방법이다. 이미 선진국의 발파현장에서는 air decking 기술을 이용한 다양한 발파방법으로 VARI-STEM, GAS-BAG, POWER DECK 등이 이용되어지고 있다. 본 연구에서는 이들 중에서 Air ball 제품을 이용한 발파공법에 대하여 연구하고 국내 현장적용과 효과에 대하여 분석하였다. 그 결과 발파진동의 경우에는 약 30 ~ 40% 정도의 감쇄효과가 나타났고, 화약량의 경우 20 ~ 25%정도의 감소를 보였다. 또한 폭음의 경우에도 Air ball을 이용한 발파가 효과적이었으나 분석결과의 결정계수가 낮아 이는 더 많은 실험을 통하여 다시 분석해야 할 것으로 생각된다.

Effect of Rice Cultural Practices on Water Percolation, Irrigation Requirement, and Nitrogen Leaching under Lysimeter Condition

Kim, Dea-wook,Chae, Je-Cheon,Kim, Sung-Woo The Korean Society of Crop Science 2001 Korean journal of crop science Vol.46 No.1

In this lysimeter experiment, temporal changes of water percolation rate, irrigation requirement and ${No}_3$--N leaching were investigated under different cultural practices that were no-till direct seeding on flooded paddy (NTDSF), till direct seeding on flooded paddy (TDSF), and transplanting. The highest water percolation rate of 3,001 l/$m^2$ was measured in NTDSF. Others were 2,551 l/$m^2$ and 2,210 l/$m^2$ in TDSF and transplanting. Water percolation rate in NTDSF and TDSF was increased by 36% and 15% compared to transplanting. Water percolation rates in all cultural practices were increased remarkably from the reproductive growth stage and relatively large amount of water loss through percolation was measured even after the reproductive growth stage. A total irrigation requirement was 3,469 l/$m^2$ in NTDSF and 2,898 l/$m^2$ in TDSF. That was equivalent to 45% and 21 % of increase compared to 2,389 l/$m^2$ in transplanting. The largest ${No}_3$--N leaching through the entire rice growing period was 701 mg/$m^2$ in NTDSF and was followed by 494 mg/$m^2$ in TDSF and 465 mg/$m^2$ in transplanting. The ratios to the total amount of ${No}_3$--N leaching at the vegetative growth stage, reproductive growth stage and ripening stage were 31 %, 41 % and 28% in NTDSF; 21 %, 48% and 31 % in TDSF; and 18%, 48% and 35 % in transplanting.

Light/Dark Responsiveness of Kinetin-Inducible Secondary Metabolites and Stress Proteins in Rice Leaf

Dea-Wook Kim 한국작물학회 2007 Journal of crop science and biotechnology Vol.10 No.2

Kinetin (KN) is an inducer of rice (Oryza sativa L.) defense/stress responses, as evidenced by the induction of inducible secondary metabolite and defense/stress protein markers in leaf. We show a novel light-dependent effect of KN-triggered defense stress responses in rice leaf. Leaf segments treated with KN (100 μM) show hypersensitive-like necrotic lesion formation only under continuous light illumination. Potent accumulation of two phytoalexins, sakuranetin, and momilactone A (MoA) by KN that peaks at 48 h after treatment under continuous light is completely suppressed by incubation under continuous dark. Using two-dimensional gel electrophoresis we identified KN-induced changes in ribulose-1, 5-bisphosphate carboxylase/oxygenase, energy- and pathogenesisrelated proteins (OsPR class 5 and 10 members) by N-terminal amino acid sequencing and mass spectrometry. These changes were light-inducible and could not be observed in the dark (and control). The present results provide a new dimension (light modulation/regulation) to our finding that KN has a potential role in the rice plant self-defense mechanism. Kinetin (KN) is an inducer of rice (Oryza sativa L.) defense/stress responses, as evidenced by the induction of inducible secondary metabolite and defense/stress protein markers in leaf. We show a novel light-dependent effect of KN-triggered defense stress responses in rice leaf. Leaf segments treated with KN (100 μM) show hypersensitive-like necrotic lesion formation only under continuous light illumination. Potent accumulation of two phytoalexins, sakuranetin, and momilactone A (MoA) by KN that peaks at 48 h after treatment under continuous light is completely suppressed by incubation under continuous dark. Using two-dimensional gel electrophoresis we identified KN-induced changes in ribulose-1, 5-bisphosphate carboxylase/oxygenase, energy- and pathogenesisrelated proteins (OsPR class 5 and 10 members) by N-terminal amino acid sequencing and mass spectrometry. These changes were light-inducible and could not be observed in the dark (and control). The present results provide a new dimension (light modulation/regulation) to our finding that KN has a potential role in the rice plant self-defense mechanism.

Gene Transcription in the Leaves of Rice Undergoing Salt-induced Morphological Changes (Oryza sativa L.)

Dea-Wook Kim,Junko Shibato,Ganesh Kumar Agrawal,Shinsuke Fujihara,Hitoshi Iwahashi,Du Hyun Kim,심이성,Randeep Rakwal 한국분자세포생물학회 2007 Molecules and cells Vol.24 No.1

2-(3-Fluoro-4-methylsulfonylaminophenyl)propanamides as Potent Transient Receptor Potential Vanilloid 1 (TRPV1) Antagonists: Structure–Activity Relationships of 2-Amino Derivatives in the <i>N</i>-(6-Trifluoromethylpyridin-3-ylmethyl) C-Region

Kim, Myeong Seop,Ryu, HyungChul,Kang, Dong Wook,Cho, Seong-Hee,Seo, Sejin,Park, Young Soo,Kim, Mi-Yeon,Kwak, Eun Joo,Kim, Yong Soo,Bhondwe, Rahul S.,Kim, Ho Shin,Park, Seul-gi,Son, Karam,Choi, Sun,DeA American Chemical Society 2012 Journal of medicinal chemistry Vol.55 No.19

<P>A series of <I>N</I>-(2-amino-6-trifluoromethylpyridin-3-ylmethyl)-2-(3-fluoro-4-methylsulfonylaminophenyl)propanamides were designed combining previously identified pharmacophoric elements and evaluated as hTRPV1 antagonists. The SAR analysis indicated that specific hydrophobic interactions of the 2-amino substituents in the C-region of the ligand were critical for high hTRPV1 binding potency. In particular, compound <B>49</B><I><B>S</B></I> was an excellent TRPV1 antagonist (<I>K</I><SUB>i(CAP)</SUB> = 0.2 nM; IC<SUB>50(pH)</SUB> = 6.3 nM) and was thus approximately 100- and 20-fold more potent, respectively, than the parent compounds <B>2</B> and <B>3</B> for capsaicin antagonism. Furthermore, it demonstrated strong analgesic activity in the rat neuropathic model superior to <B>2</B> with almost no side effects. Compound <B>49</B><I><B>S</B></I> antagonized capsaicin induced hypothermia in mice but showed TRPV1-related hyperthermia. The basis for the high potency of <B>49</B><I><B>S</B></I> compared to <B>2</B> is suggested by docking analysis with our hTRPV1 homology model in which the 4-methylpiperidinyl group in the C-region of <B>49</B><I><B>S</B></I> made additional hydrophobic interactions with the hydrophobic region.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jmcmar/2012/jmcmar.2012.55.issue-19/jm300780p/production/images/medium/jm-2012-00780p_0014.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jm300780p'>ACS Electronic Supporting Info</A></P>

Responses of antioxidant enzymes and their corresponding genes in rice leaf under salt stress

Kim Dea-Wook,Heo Hwa-Young,Rakwal Randeep,Shim Ie-Sung 한국작물학회 2006 한국작물학회 학술발표대회 논문집 Vol.2006 No.-

Evaluation and Verification of Barley Genotypes with Known Genes for Resistance to Barley yellow mosaic virus and Barley mild mosaic virus Under Field Conditions in South Korea

Kim, Hong-Sik,Baek, Seong-Bum,Kim, Dea-Wook,Hwang, Jong-Jin,Kim, Si-Ju The Korean Society of Plant Pathology 2011 Plant Pathology Journal Vol.27 No.4

Soil-borne barley yellow mosaic disease caused by Barley yellow mosaic virus (BaYMV) or Barley mild mosaic virus (BaMMV) gives a serious threat to the winter barley cultivated in the southern regions in Korea. It is important to develop resistant varieties for stable and high-yield production. The objectives of this study were to evaluate 22 genotypes of exotic barley germplasms carrying the resistance genes rym1 through rym12, with the exception of rym10, and to determine the genes that confer resistance to BaYMV or BaMMV in Korea. Using the traditional visual scoring of symptoms at 4 locations over 3 years, average disease rate values differed (P < 0.001) among the genotypes. ELISA test revealed the presence of both BaYMV and BaMMV in all of the field sites but Jinju and significantly different rates of infection among genotypes and years. Barley genotypes differed in how virus quantities and pathogen-induced symptoms were correlated, especially in response to BaYMV. Disease incidence was affected by the climatic conditions present during the early growing stage before overwintering. A Chinese landrace, 'Mokusekko 3', carrying rym1 and rym5 was comparatively resistant at all locations studied. The barley genotypes carrying either rym6 or rym9 were susceptible to the viral strains. The genotypes carrying rym5 were resistant in Jinju and Milyang but susceptible in Iksan and Naju. The resistance genes rym2 and rym3 were effective in local strains and would be potent contributors to disease resistance.

Inabenfide-Induced Alleviation of Salt Stress in Rice as Linked to Changes in Salicylic Acid Content and Catalase Activity

Dea-Wook Kim,Hiroko Sawada,Katsuichiro Kobayashi,Ie-Sung Shim 한국작물학회 2007 Journal of crop science and biotechnology Vol.10 No.1

The effect of inabenfide was investigated in rice seedlings subjected to salt stress in relation to changes in chlorophyll fluorescence (Δ F/Fm’), lipid peroxidation, salicylic acid (SA) content, and catalase (CAT) activity. A reduction in shoot growth of rice seedlings by 120 mM NaCl treatment was significantly alleviated by pretreatment with 30 μM inabenfide. Sodium ion content was not affected by pretreatment with inabenfide, suggesting that alleviation was not due to a reduction in sodium ion uptake by the rice seedlings. At three days after NaCl treatment, the rice seedlings pretreated with inabenfide showed a higher ΔF/Fm’ (30%) and lower lipid peroxidation (28%) compared with the rice seedlings treated with NaCl alone. After NaCl treatment, CAT activity in the third leaf of rice seedlings decreased significantly but alleviated by pretreatment with inabenfide. Furthermore, pretreatment with inabenfide also reduced the level of SA which accumulated drastically in the third leaf of rice seedlings within a day after exposure to salt stress. These results suggest that inabenfide prevents SA accumulation in rice seedlings under salt stress which eventually induces the alleviation of salt stress damage. The effect of inabenfide was investigated in rice seedlings subjected to salt stress in relation to changes in chlorophyll fluorescence (Δ F/Fm’), lipid peroxidation, salicylic acid (SA) content, and catalase (CAT) activity. A reduction in shoot growth of rice seedlings by 120 mM NaCl treatment was significantly alleviated by pretreatment with 30 μM inabenfide. Sodium ion content was not affected by pretreatment with inabenfide, suggesting that alleviation was not due to a reduction in sodium ion uptake by the rice seedlings. At three days after NaCl treatment, the rice seedlings pretreated with inabenfide showed a higher ΔF/Fm’ (30%) and lower lipid peroxidation (28%) compared with the rice seedlings treated with NaCl alone. After NaCl treatment, CAT activity in the third leaf of rice seedlings decreased significantly but alleviated by pretreatment with inabenfide. Furthermore, pretreatment with inabenfide also reduced the level of SA which accumulated drastically in the third leaf of rice seedlings within a day after exposure to salt stress. These results suggest that inabenfide prevents SA accumulation in rice seedlings under salt stress which eventually induces the alleviation of salt stress damage.

Effect of Rice Cultural Practices on Water Percolation, Irrigation Requirement, and Nitrogen Leaching under Lysimeter Condition

Dea-wook Kim,Je-Cheon Chae,Sung-Won Kim 韓國作物學會 2001 Korean journal of crop science Vol.46 No.1

In this lysimeter experiment, temporal changes of water percolation rate, irrigation requirement and No3 --N leaching were investigated under different cultural practices that were no-till direct seeding on flooded paddy (NTDSF), till direct seeding on flooded paddy (TDSF), and transplanting. The highest water percolation rate of 3,001 l/m2 was measured in NTDSF. Others were 2,551 l/m2 and 2,210 l/m2 in TDSF and transplanting. Water percolation rate in NTDSF and TDSF was increased by 36% and 15% compared to transplanting. Water percolation rates in all cultural practices were increased remarkably from the reproductive growth stage and relatively large amount of water loss through percolation was measured even after the reproductive growth stage. A total irrigation requirement was 3,469 l/m2 in NTDSF and 2,898 l/m2 in TDSF. That was equivalent to 45% and 21 % of increase compared to 2,389 l/m2 in transplanting. The largest No3 --N leaching through the entire rice growing period was 701 mg/m2 in NTDSF and was followed by 494 mg/m2 in TDSF and 465 mg/m2 in transplanting. The ratios to the total amount of No3 --N leaching at the vegetative growth stage, reproductive growth stage and ripening stage were 31 %, 41 % and 28% in NTDSF; 21 %, 48% and 31 % in TDSF; and 18%, 48% and 35 % in transplanting.

Proteomic approach for analyzing cellular responses to abiotic stresses in crop plants

Kim Dea-Wook,Agrawal Ganesh Kumar,Rakwal Randeep 한국작물학회 2007 한국작물학회 학술발표대회 논문집 Vol.2007 No.-