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Park, Jisoo,Baik Kim, Gae,Lippitz, Andreas,Kim, Young Mi,Jung, Donggeun,Unger, Wolfgang E.S.,Kim, Young-Pil,Lee, Tae Geol Elsevier 2019 Sensors and actuators. B Chemical Vol.281 No.-
<P><B>Abstract</B></P> <P>We report polyethylene glycol (PEG)-grafting antifouling surfaces using a plasma copolymerized (PcP) technique to monitor protease activity in complex media. By varying the mixing ratio of the PEG and ethylenediamine (EDA) precursors, the PcP-PEG-EDA (PcP-PE) film was able to easily control surface amine density with good preservation of the internal PEG structure. We found that nonspecific protein adsorption was dramatically reduced in serum-containing media on the PcP-PE films, as opposed to that on plasma polymerized-EDA (PP-E) films without PEG. When SPR sensor chips coated with PcP-PE film were employed to detect protease activity, biotinylated luciferase probes (luciferase-peptide-biotin) on streptavidin-conjugated SPR chips enabled real-time and label-free measurement of matrix metalloproteinase activity in cell culture media. Owing to its excellent antifouling ability, this newly developed method boasts minimal nonspecific binding and can serve as a biochip platform to promote a wide range of applications in the biological field.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Plasma copolymerized (PcP) method with PEG/EDA created antifoulding surfaces. </LI> <LI> Protease activity in cell culture media was detected on a PcP/SPR sensor chip. </LI> <LI> This approach will be useful for bioassays with minimal nonspecific binding. </LI> </UL> </P>
Peptide-directed Photocrosslinking for Site-specific Conjugation of Native Antibody
Jisoo PARK,Tae Hyeon YOO 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Antibody-based conjugates have become an essential component in a variety of applications from immunoassays especially to drug conjugates because of their proven efficacy and selectivity to target cancer cell. Herein, we describe a peptide-directed photo-crosslinking reaction as a novel site-specific conjugation method of IgG using an Fc-binding peptide and a photoreactive amino acid analogue for generation homogeneous IgG-toxin conjugates.
Affinity Peptide Directed Site-specific Conjugation of IgG via a Facile Photocrosslinking
Jisoo PARK,Yeaji LEE,Tae Hyeon YOO 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Antibody-based conjugates have become an essential component in a variety of applications from immunoassays especially to drug conjugates because of their proven efficacy and selectivity to target cancer cell. Herein, we describe a peptide-directed photo-crosslinking reaction as a novel site-specific conjugation method of IgG using an Fc-binding peptide and a photoreactive amino acid analogue for generation homogeneous IgG-toxin conjugates.
Recognition of Transmembrane Protein 39A as a Tumor-Specific Marker in Brain Tumor
Park, Jisoo,Lee, Hyunji,Tran, Quangdon,Mun, Kisun,Kim, Dohoon,Hong, Youngeun,Kwon, So Hee,Brazil, Derek,Park, Jongsun,Kim, Seon-Hwan Korean Society of ToxicologyKorea Environmental Mu 2017 Toxicological Research Vol.33 No.1
Transmembrane protein 39A (TMEM39A) belongs to the TMEM39 family. TMEM39A gene is a susceptibility locus for multiple sclerosis. In addition, TMEM39A seems to be implicated in systemic lupus erythematosus. However, any possible involvement of TMEM39A in cancer remains largely unknown. In the present report, we provide evidence that TMEM39A may play a role in brain tumors. Western blotting using an anti-TMEM39A antibody indicated that TMEM39A was overexpressed in glioblastoma cell lines, including U87-MG and U251-MG. Deep-sequencing transcriptomic profiling of U87-MG and U251-MG cells revealed that TMEM39A transcripts were upregulated in such cells compared with those of the cerebral cortex. Confocal microscopic analysis of U251-MG cells stained with anti-TMEM39A antibody showed that TMEM39A was located in dot-like structures lying close to the nucleus. TMEM39A probably located to mitochondria or to endosomes. Immunohistochemical analysis of glioma tissue specimens indicated that TMEM39A was markedly upregulated in such samples. Bioinformatic analysis of the Rembrandt knowledge base also supported upregulation of TMEM39A mRNA levels in glioma patients. Together, the results afford strong evidence that TMEM39A is upregulated in glioma cell lines and glioma tissue specimens. Therefore, TMEM39A may serve as a novel diagnostic marker of, and a therapeutic target for, gliomas and other cancers.
Park, DoYeun,Park, JiSoo,Jang, Heeyeong,Cheng, Jie,Hyun Kim, Soo,Lee, Sang-Hoon IOP Publishing 2017 Biofabrication Vol.9 No.2
<P>Microfibers produced using electrospinning and microfluidics-based technologies have been developed as a powerful tool in tissue engineering applications such as drug delivery and scaffolds. The applications of these fibers, however, have been limited because of the hazardous solvents used to make them, difficulties in controlling the pore sizes of their membrane forms, and downscaling the size of the fiber. Nevertheless, extending the use of these fibers, for example in the production of a freestanding porous membrane appropriate for cell-based research, is highly needed for tissue engineering, organ-on-a-chip, and drug delivery research and applications. Here, we fabricated a freestanding porous membrane by using a novel method that involved simultaneously spinning multiple strands of submicron-thick 'noodle-like' fibers. In addition to the novelty of the single noodle fiber in overcoming the size-reducing limitations of conventional microfluidic spinning methods, these fibers can hence form the units of 'noodle membranes' whose pores have sizes that the convention electrospinning method cannot achieve. We confirmed the potential of the noodle membrane to serve as a free-standing porous membrane in two simple experiments. Also, we found that noodle membranes have an advantage in loading different amounts of different materials in itself that it was also shown to be of use as a new type of scaffold for complex tissue regeneration. Therefore, the proposed noodle membrane can be an effective tool in tissue engineering applications and biological studies.</P>
Increased phosphorylation of Ca(2+) handling proteins as a proarrhythmic mechanism in myocarditis.
Park, Hyelim,Park, Hyewon,Lee, Dajeong,Oh, Sujung,Lim, Jisoo,Hwang, Hye Jin,Park, Sungha,Pak, Hui-Nam,Lee, Moon-Hyoung,Joung, Boyoung Japanese Circulation Society 2014 CIRCULATION JOURNAL Vol.78 No.9
<P>Because fatal arrhythmia is an important cause of death in patients with myocarditis, we investigated the proarrhythmic mechanisms of experimental autoimmune myocarditis. METHODS???AND???RESULTS: Myocarditis was induced by injection of 2 mg porcine cardiac myosin into the footpads of adult Lewis rats on days 1 and 8 (Myo, n=15) and the results compared with Control rats (Control, n=15). In an additional 15 rats, 6 mg/kg prednisolone was injected into the gluteus muscle before the injection of porcine cardiac myosin on days 1 and 8 (MyoS, n=15). Hearts with myocarditis had longer action potential duration (APD), slower conduction velocity (CV; P<0.01 vs. Control), higher CV heterogeneity, greater fibrosis, higher levels of immunoblotting of high-mobility group protein B1, interleukin 6 and tumor necrosis factor-관 proteins. Steroid treatment partially reversed the translations for myocarditis, CV heterogeneity, reduced APD at 90% recovery to baseline, increased CV (P<0.01), and reversed fibrosis (P<0.05). Programmed stimulation triggered sustained ventricular tachycardia in Myo rats (n=4/5), but not in controls (n=0/5) or Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) inhibitor (KN93) treated Myo rats (n=0/5, P=0.01). CaMKII autophosphorylation at Thr287 (201%), and RyR2 phosphorylation at Ser2808 (protein kinase A/CaMKII site, 126%) and Ser2814 (CaMKII site, 21%) were increased in rats with myocarditis and reversed by steroid.</P>
Alcohol Intoxication and Glasgow Coma Scale Scores in Patients with Head Trauma
( Jisoo Park ),( Taejin Park ),( Jung-in Ko ),( Woonhyung Yeo ) 대한외상학회 2020 大韓外傷學會誌 Vol.33 No.4
Purpose: Alcohol intoxication is commonly associated with traumatic brain injury (TBI), but the inf luence of alcohol on the Glasgow Coma Scale (GCS) score remains unclear. This study investigates the effects of blood alcohol concentration (BAC) on the GCS score in head trauma patients with alcohol intoxication. Methods: In total, 369 head trauma patients with alcohol intoxication in a 1-year period were retrospectively analyzed. The patients underwent head computed tomography and had a BAC ≥80 mg/dL. Patients were divided into TBI and non-TBI groups. Brain injury severity was further classified using the head Abbreviated Injury Score (AIS). The effects according to 5 BAC groups were examined. Results: The TBI group consisted of 64 patients (16.2%). The mean BAC was significantly higher in the non-TBI group (293.4±87.3 mg/dL) than in the TBI group (242.8±89.9 mg/dL). The mean GCS score was significantly lower in the TBI group (10.3±4.6) than in the non-TBI group (13.0±2.5). A higher BAC showed a significant association with a lower mean GCS score in the TBI group, but not in the non-TBI group. Above ≥150 mg/dL, higher BACs showed significant odds ratios for a lower GCS score. Conclusions: The influence of alcohol in patients with head trauma depended on the presence of a brain injury. An association between a higher BAC and a lower GCS score was only observed in patients with TBI. Therefore, if a severe brain injury is suspected based on a GCS evaluation in patients with alcohol intoxication, prompt diagnosis and intensive care should be performed without delay.