Suppression of IPSP by Priming Stimulation in Rat Piriform Cortex

Jung, Min Whan,Mook-Jung, Inhee 아주대학교 1997 아주의학 Vol.2 No.1

High frequency burst stimulation repeated at theta (about 5Hz) frequency is optimal for induction of LTP in hippocampus. TBS is effective since fast IPSP is suppressed by priming stimulation maximal at around 200 ms interval, which results in a large voltage summation and hence activation of NMDA receptors in the postsynaptic neuron. LTP is reliably induced by TBS in piriform cortex, suggesting that the same cellular process (IPSP suppression) may operate in piriform cortex. To test this possibility, effects of priming stimulation on IPSP suppression were examined in the rostral piriform cortex. Intracellular recordings were made from layer H pyramidal neurons, and electrical stimulation was delivered to the intrinsic associational fibers. Both heterosynaptic and homosynaptic priming stimulation induced prolongation of subsequent synaptic responses evoked 200 ms later. However, heterosynaptic stimulation did not affect the peak amplitude of the subsequent synaptic responses, indicating that the effect is not due to other forms of synaptic plasticity such as paired-pulse facilitation. When the same experiments were repeated while the postsynaptic neurons were depolarized by current injection, suppression of fast IPSP by priming stimulation was clearly discernable. Burst stimulation resulted in a large temporal summation of EPSPs for primed burst responses, an ideal condition for activation of NMDA receptors. These results indicate that cellular mechanisms for IPSP suppression by priming stimulation indeed exist in piriform cortex as in hippocampus, and this is probably the reason why TBS is effective for induction of LTP in piriform cortex. Unlike hippocampus, however, homosynaptic stimulation was more effective than heterosynaptic stimulation for suppression of fast IPSP, and maximal effect was around 100 ms interval, suggesting that the optimal frequency and operational modes are different in the two structures.

Molecular Pathogenesis of Alzheimer`s Disease

Jung, Inhee-Mook 한림대학교 환경·생명과학연구소 2000 [일송 국제심포지엄] 노화와 만성퇴행성 신경질환 Vol.- No.3

Identification of <i>Drosophila</i> SOD3 and its protective role against phototoxic damage to cells

Jung, Inhee,Kim, Tae-Yoon,Kim-Ha, Jeongsil Elsevier 2011 FEBS letters Vol.585 No.12

<P><B>Highlights</B></P><P>► A putative SOD3 gene sequence (dSod3) can be found in the <I>Drosophila</I> genome. ► dSod3 protein retains SOD activity and is secreted as soon as it is synthesized. ► dSod3 is required for both resistance to oxidative stress and longevity in adult flies. ► The dSod3 protein protects cells from UV-induced cell death.</P> <P><B>Abstract</B></P><P>Superoxide dismutase (SOD) is one of several major proteins that regulate removal of superoxide. Three isoforms of SOD exist in mammals. It has long been thought that <I>Drosophila</I> lacks the SOD3 gene. However, a putative SOD3 gene sequence (dSod3) in the <I>Drosophila</I> genome was reported recently. Thus we investigated whether dSod3 truly functions as a SOD3 homolog in <I>Drosophila</I>. We found that dSod3 not only retains SOD activity but also properties of secreted proteins, as do other SOD3s. In addition, the dSod3 protein alleviates ultraviolet-induced cellular damage. These results suggest that dSod3 functions as an extracellular SOD3.</P>

알쯔하이머병 환자 혈청에서의 베타 아밀로이드 단백질에 대한 특이 항체량 측정 : 알쯔하이머병의 생화학적 진단지표 개발 Development of Biomarker for Alzheimer's Disease Diagnosis

소정온,허지연,심혜진,김종원,나덕렬,이필휴,정선주,박문호,주인수,송미숙,김영호,묵인희 대한치매학회 2004 Dementia and Neurocognitive Disorders Vol.3 No.1

Background:Alzheimer's disease (Ad) is a neurodegenerative disorder that is rapidly increasing with the aging society, requiring a need for early diagnosis and prevention. However, diagnosis on AD has only been possible through limited methods such as neuropsychological examination or MRI. AD is characterized by deposition of senile plaques and neurofibrillary tangles in the brain. Aβ peptide in senile plaques seems to play a central role in the neuropathology of AD. Several biochemical markers for AD are available, including reduced Aβ protein, a change in ratio between Aβ40 and 42 and increased level of tau protein in the cerebrospinal fluid (CSF). Methods:This study analyzes anti-Aβ antibody from serums of AD patients using the ELISA. The levels of anti-Aβ antibody from patients with idiopathic Parkinson's disease or stroke and from normal control were compared to that of AD patients. Results:Our results showed a significantly lower anti-Aβ antibody level in AD compared to those with other neurological diseases or control. Conclusions:These data showed that the anti-Aβ antibody level in the serum may be used to diagnose the presence of AD.

Mitochondrial abnormalities related to the dysfunction of circulating endothelial colony-forming cells in moyamoya disease

Choi, Jung Won,Son, Sung Min,Mook-Jung, Inhee,Moon, Youn Joo,Lee, Ji Yeoun,Wang, Kyu-Chang,Kang, Hyun-Seung,Phi, Ji Hoon,Choi, Seung Ah,Chong, Sangjoon,Byun, Jayoung,Kim, Seung-Ki Journal of Neurosurgery Publishing Group 2018 Journal of neurosurgery Vol.129 No.5

<B>OBJECTIVE</B><P>Moyamoya disease (MMD) is a unique cerebrovascular disorder characterized by the progressive occlusion of the bilateral internal carotid arteries. Endothelial colony-forming cells (ECFCs), previously termed “endothelial progenitor cells,” play an important role in the pathogenesis of MMD. In this study, the authors performed morphological and functional studies of the mitochondria of ECFCs from patients with MMD to present new insights into the pathogenesis of the disease.</P><B>METHODS</B><P>The morphology of ECFCs from 5 MMD patients and 5 healthy controls was examined under both a transmission electron microscope and a confocal laser scanning microscope. The oxygen consumption rates (OCRs), mitochondrial membrane potentials (MMPs), intracellular Ca<SUP>2+</SUP> concentrations, mitochondrial enzyme activities, and reactive oxygen species (ROS) levels were measured. Functional activity of the ECFCs was evaluated using a capillary tube formation assay.</P><B>RESULTS</B><P>The ECFCs from the MMD patients displayed a disrupted mitochondrial morphology, including a shorter and more circular shape. The ECFC mitochondria from the MMD patients exhibited functional abnormalities, which were assessed as a decreased OCR and an increased intracellular Ca<SUP>2+</SUP> concentration. Moreover, the ECFCs from MMD patients showed increased ROS levels. Interestingly, treatment with an ROS scavenger not only reversed the mitochondrial abnormalities but also restored the angiogenic activity of the ECFCs from the MMD patients.</P><B>CONCLUSIONS</B><P>The mitochondria of ECFCs from MMD patients, as compared with those from healthy patients, exhibited morphological and functional abnormalities. This finding suggests that the mitochondrial abnormalities may have a role in the pathogenesis of MMD.</P>

협동조합 브랜드 아이덴티티 연구 ; 식음업종 사례를 중심으로

정인희 ( Inhee Jung ),이진민 ( Jinmin Rhie ),장미정 ( Mijung Jang ) 한국산업디자이너협회(한국인더스트리얼디자인학회) 2016 산업디자인학연구 Vol.10 No.3

본 연구는 협동조합과 브랜드 아이덴티티의 이해를 그 기초에 두고 협동조합 특성에 맞는 브랜드 아이덴티티의 표현요소와 의미요소를 도출하여, 협동조합의 브랜드 아이덴티티를 구축하기 위한 방안을 모색 하는데 목적이 있다. 본 연구의 범위는 시간적 범위, 대상적 범위로 나눈다. 첫째, 시간적 범위는 2012년 12월 1일 협동조합 제정법이 시작 된 이후부터 2015년 10월 31일까지로 한다. 둘째, 대상적 범위는 국내사례와 해외사례로 나눈다. 국내사례는 총회의 의결을 거쳐 기획재정부 협동조합 홈페이지에 게재된 식음업종의 협동조합으로 선정한다. 해외사례는 ICA와 Euricse가 공동 발표한 ‘세계협동조합 모니터 2014’에 보고된 65개국 중에 MBN에서 방영된 협동프로젝트 ‘신 부자수업’의 해외사례들 중에 식음브랜드를 가지고 있는 협동조합으로 선정한다. 본 연구의 방법은 질적연구 방법으로서 국내와 해외의 협동조합브랜드아이덴티티를 비교분석하고 이를 표현요소와 의미요소로 나누어 특성을 파악한다. 표현요소는 그래픽 아이덴티티(2D), 제품 아이덴티티(3D), 공간 아이덴티티(4D)의 측면으로 연구 설계하고 이를 컬러 이미지스케일에 대입하여 분석한다. 의미요소는 제품으로서의 브랜드, 조직으로서의 브랜드. 사람으로서의 브랜드, 상징으로서의 브랜드 측면으로 연구 설계하고 형용사 이미지스케일에 대입하여 분석한다. 끝으로 본 연구는 협동조합의 브랜드 아이덴티티 구축을 위한 마케팅의 표현요소 및 의미요소와 디자인의 평면-입체-공간과의 연계성을 도출하였다. 이는 향후 국내 협동조합의 브랜드 아이덴티티 구축 및 확장을 위한 기초적 자료로서의 활용가치가 있다. The purpose of this study is to seek ways to establish brand identity for cooperatives, by deducting the elements of expression and meaning suited to understanding of cooperatives and brand identity, and characteristics of cooperatives.This study is divided into temporal scope and object scope. First, temporal scope is limited to scope to analyze study case from Cooperatives Act in December 1 2012 to October 31 2015. Second, objective scope is divided into domestic and foreign cases. The scope of domestic case is limited to food cooperatives published on public announcement of business management. The scope of foreign case is cooperatives with food and beverage brand among foreign cases of ‘New Rich Class’, a co-operating project televised by MBN. As an analytical method of the study, it is designed by deducting brand identity for cooperatives in terms of both expression element and meaning element. First, in expression element, a study is designed in various terms such as graphic identity(2D), product identity(3D), and spatial identity(4D), and is analyzed by substituting image scale of Kobayashi. Second, in meaning element, a study is designed in terms of brand as product, brand as organization, brand as person, and brand as symbol in ‘brand identity system’ of Aaker, and is analyzed by substituting adjective scale of Kobayashi. Last, connectivity of expression element and meaning element based on analytical data is deducted, and it is meaningful to establish brand identity of cooperatives by raising design value.

영한 영상번역 전략 연구

정인희(Jung Inhee) 한국번역학회 2006 번역학연구 Vol.7 No.2

Upregulation of amyloid precursor protein by platelet-derived growth factor in hippocampal precursor cells :

Lim, Jung Su,Cho, Hyunjung,Hong, Hyun Seok,Kwon, Hyockman,Mook-Jung, Inhee,Kwon, Yunhee Kim Ovid Technologies (Wolters Kluwer) - Lippincott Wi 2007 NEUROREPORT - Vol.18 No.12

<P>Amyloid precursor protein generates the secreted amyloid precursor protein alpha, which protects hippocampal neurons from ischemic injury and facilitates neuronal survival and synaptogenesis in the developing nervous system. Here, we examined whether platelet-derived growth factor regulates the generation of secreted amyloid precursor protein alpha during the neuronal differentiation of hippocampal precursor cells, HiB5. We showed that platelet-derived growth factor promoted amyloid precursor protein production and secreted amyloid precursor protein alpha secretion. These effects of platelet-derived growth factor were diminished by the PI3K-specific inhibitor wortmannin and the protein kinase C-specific inhibitor GF109203X, suggesting the involvement of the PI3K and protein kinase C-signaling pathway. Furthermore, the conditioned media enriched with secreted amyloid precursor protein alpha promoted the survival of HiB5 cells during neuronal differentiation. These results suggest that the neurotrophic effect of platelet-derived growth factor is mediated in part via upregulation of the expression and release of secreted amyloid precursor protein alpha.</P>

Transgenic potato expressing Aβ reduce Aβ burden in Alzheimer’s disease mouse model

Youm, Jung Won,Kim, Hee,Han, Jee Hye Lo,Jang, Chang Hwan,Ha, Hee Jin,Mook-Jung, Inhee,Jeon, Jae Heung,Choi, Cheol Yong,Kim, Young Ho,Kim, Hyun Soon,Joung, Hyouk Elsevier 2005 FEBS letters Vol.579 No.30

<P><B>Abstract</B></P><P>Beta amyloid (Aβ) is believed one of the major pathogens of Alzheimer’s disease (AD), and the reduction of Aβ is considered a primary therapeutic target. Immunization with Aβ can reduce Aβ burden and pathological features in transgenic AD model mice. Transgenic potato plants were made using genes encoding 5 tandem repeats of Aβ1–42 peptides with an ER retention signal. Amyloid precursor protein transgenic mice (Tg2576) fed with transgenic potato tubers with adjuvant showed a primary immune response and a partial reduction of Aβ burden in the brain. Thus, Aβ tandem repeats can be expressed in transgenic potato plants to form immunologically functional Aβ, and these potatoes has a potential to be used for the prevention and treatment of AD.</P>

Proteomic Profiling Reveals Upregulated Protein Expression of Hsp70 in Keloids

Lee, Ju Hee,Shin, Jung U.,Jung, Inhee,Lee, Hemin,Rah, Dong Kyun,Jung, Jin Young,Lee, Won Jai Hindawi Publishing Corporation 2013 BioMed research international Vol.2013 No.-

<P><I>Background.</I> The biochemical characteristics of keloid-derived fibroblasts differ from those of adjacent normal fibroblasts, and these differences are thought to be the cause of abnormal fibrosis. Therefore, we investigated the characteristic proteins that are differentially expressed in keloid-derived fibroblasts using proteomics tools. <I>Objective.</I> We attempted to investigate the novel proteins that play important roles in the pathophysiology of keloids. <I>Methods.</I> Proteomics analysis was performed to identify differentially expressed proteins in keloid-derived fibroblasts. Keloid-derived fibroblasts and adjacent normal fibroblasts were analyzed with 2-DAGE. We validated these proteins with immunoblot analysis, real-time RT-PCR, and immunohistochemistry. <I>Results.</I> Sixteen differentially expressed protein spots were identified in keloid-derived fibroblasts. Among them, heat shock protein 70 (Hsp70) was specifically upregulated in keloid-derived fibroblasts. Also, immunohistochemistry and western blot analysis revealed increased Hsp70, TGF-<I><I>β</I></I>, and PCNA expressions in keloids compared to normal tissue. <I>Conclusion.</I> Hsp70 is overexpressed in keloid fibroblasts and tissue. The overexpression of Hsp70 may be involved in the pathogenesis of keloids, and the inhibition of Hsp70 could be a new therapeutic tool for the treatment of keloids.</P>