악성 흉막삼출액에서 Bleomycin을 이용한 흉막유착설의 시술효과

박정현,김학렬,양세훈,정은택 圓光大學校 醫科學硏究所 2002 圓光醫科學 Vol.17 No.1

목적: 악성 흉막삼출액이 반복적이고 지속적으로 발생하는 경우, 호흡곤란, 흉통등의 증상을 유발시키므로 흉막경화제에 의한 흉막유착술을 시행할 수 있다. 흔히 사용되던 talc의 심각한 부작용이 보고되고 있고, doxycycline 주사제도 국내에서 발매되지 않고 있다. 이에 연자들은 항암제로 소개된 bleomycin을 이용한 흉막유착술의 단기와 중기효과, 부작용 및 생존율 등을 전향적으로 확인하였다. 방법: 2000년 1월부터 2001년 12열까지 원광대학교 부속병원에 내원하여 반복적이고 지속적인 악성 흉막삼출액으로 흉막유착술이 필요한 26명의 환자를 대상으로 특별한 전 처치 없이 bleomycin 60 unit를 식염수와 함께 흉강 내 주입 후, 1일 후의 흉관에 의한 배액량을 확인하고 부작용을 관찰하였으며, 1개월 후에 흉부 방사선상으로 재 저류 유무를 확인하였다. 또한 시술 반응군과 비반응군으로 나누어 중앙생존기간을 비교분석 하였다. 결과: 시술 1일 후 26예중 10예(38%)에서 배액이 없었고, 8예(31%)에서 배액량이 100ml이하였으며, 8예(31%)는 100ml이상이었으나 시술 전보다 감소하였으며 증가된 경우는 없었다. 유의할만한 부작용은 4예(고열 3예, 흉통 1예)에서 발생하였으나, 대증요업으로 조절되었다. 시술 1개월 후 흉부 방사선과 26예중 9예(35%)는 재 저류액이 없었고, 10예(38%)는 저류액이 있으나 시술 전보다 감소하였으며, 1예(4%)는 이전과 변화가 없었고, 6예(23%)는 시술 전보다 저류액이 증가하였다. 시술 후 단기 및 중기효과를 반응군과 비반응군으로 나누어 중앙생존기간으로 양군간을 비교하였을 때, 반응군에서 연장이 되었으나 통계적으로 유의한 차이는 없었다. 결론: 이상의 결과로서 bleomycin에 의한 흉막유착술은 다른 경화제보다 특별한 전 처치가 필요 없는 간편하고, 비교적 부작용이 적은 안전한 방법이며, 효과적인 치료성적을 보이는 시술로 사료된다. Background : Malignant pleural effusions are common and significant problems in patients with advanced malignancy. Repeated thoracentesis provides temporary symptomatic improvement, but most patients progressively cause dyspnea or pleuritic pain. So pleurodesis with sclerosing agent is effective as a palliative treatment. There are many drugs used as pleural sclerosing agents. But doxycycline hasn't been used in Korea since 1999. And recently talc has been reported serious side effects. In this study, pleurodesis with bleomycin was prospectively investigated for its short term and middle period effects and its side effects. Methods : Twenty-six patients with malignant pleural effusion were prospectively analyzed to estimate the effects of bleomycin pleurodesis. Without special management before the procedure, 60 units of bleomycin in 50-100ml of normal saline were instilled into the pleural space via a small bore catheter(8-10 F). Andthen repeated positional changes and rotations were done for 2 hours. We measured the drainage amount and evaluated the side effects after 24 hours and checked the existence of the effusion by chest radiography after 1 month. Results : After 24 hours, the drainage amount showed that the responder group(none or ≤ 100ml) was 69%, and that the nonresponder group(> 100ml) was 31%. Side effects (high fever in 3 cases and severe pleuritic pain in 1 case) were 15%(4/26), but patients recovered easily by symptomatic management. After 1 month, chest radiography showed that the response rate(complete or partial response) was 73%. Differences of survival times between responder and nonresponder group was not statistically significant. Conclusions : We are suggest that bleomycin as a pleural sclerosing agent is a relatively simple, safe and effective agent. But a prospective study with a larger number of patients must be warranted.

폐암으로 오인된 이물질에 의한 기관지 육아종증 1예

정종훈,김소영,김휘정,김학렬,양세훈,정은택 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1

한국산 가시톡토기과의 분류학적 검토

이병훈,이원구,김학렬 全北大學校 生物學硏究所 1984 生物學硏究年報 Vol.5 No.-

A preliminary test concerning variabilities of some Tomoceridae species of collembola has been made using scanning electron microscopy and enzyme analysis. No definite taxono mical characters were detected in the integument of 6 species belonging 2 subgenera of Tomocerus genus. The zymograms obtained by electrophoresis, however, revealed that th subgeneric characters at times should be questioned for its monophyletic meaning as suggested by those of Tomocerus (Tomocerus) kinoshitai showing a particular pattern and Tomocerus (Tomocerina) liliputanus for its apprectable variability. The distinctive zymograms of T.laxalamella might also justify the creation of a new subgenus when considered in combination with the morphological characteristics of mucron. Neither population nor interspecific difference were observed, however, in and between T. cuspidatus and T. ocreatus, which would remain problematic and necessitate further works to confirm. Tomocerus laxalamella and T. liliputanus showed more of less variabilites whereas Homidia koreana of Entomobryidae, used as out-group for comparison, displayed a prominent individual variations in a few loci.

자발성 기흉을 동반한 폐림프관평활근종증 1예

안용환,정종훈,김소영,김휘정,김학렬,양세훈,최순호,문형배,조향정,정은택 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1

Metabolism of Tyrosine and Leucine during Metamorphosis of Bombyx mori L.

Kim, Hak-Ryul,Seo, Eul-Won 한국곤충학회 1982 Korean journal of entomology Vol.12 No.1

누에 (Bombyx mori)의 종령기 및 용시기에 따른 tyrosine과 leucine의 대사를 규명하기 위하여 혈강에 $^{14}$ C-tyrosine과 $^{14}$ C-leucine을 주입시킨 후 일정한 간격으로 혈림프, 지방체, 중장 및 큐리클내의 tyrosine과 leucine의 방사능을 Beckman LS-100C Liquid Scintillation Counter로 측정하였다. 혈림프내 tyrosine의 방사능은 종령중기와 용화직후 가장 높은 농도를 보여주었으나, leucine은 전 단계에 걸쳐 점진적으로 증가하였다. 이러한 두 종류의 아미노산은 대부분 혈장내에 유리아미노산이나 이의 대사물질로 존재하고 있으며, 종령기동안 상당량의 tyrosine이 혈구내에 저장되어있다. 지방체에서 tyrosine의 농도는 비교적 낮았으나, leucine은 용화직후 가장 높은 peak를 보여주었다. 중장의 tyrosine은 용초기에 높은 peak를 나타내었으나, leucine은 종령말기와 용화직후에 높은 농도를 보여주었다. 큐리클의 tyrosine의 방사능은 조령말기와 용화직후 높은 농도를 나타내고있는 반면, leucine은 비교적 일정한 수준을 유지하였다. The metabolism of tyrosine and leucine during last instar larval and pupal stages in Bombyx mori L. was determined by Injection of $\^$14/C-tyrosine and $\^$14/C-leucine into the haemocoel of newly 5th instar larvae and then measurment of radioactivity in haemolymph, fat body, midgut, and cuticle at proper intervals in Beckman LS-100C Liquid Scintilation Counter. The radioactivity of tyrosine in the haemolymph reaches the peaks at middle 5th instar stage and just after pupation each but that of leucine shews a linear increase over the whole stage. These two injected amino acids are mostly present in haemolymph plasma as free amino acids or its metabolites but a lot of tyrosine is also contained in haemocyte during last larval stage. In fat body, tyrosine is relatively low in concentration but leucine shows the highest peak during pupation period. In midgut, tyrosine has one peak during pupation period whereas leucine shows two peaks at late last larval stage and at pupation period. The radioactivity of tyrosine in cuticle reaches two peaks, first at late last instar stage and second the most highest peak just after pupation whereas leucine shows rather constant level.

Changes of RNA and Protein Content in Epidermis during Metamorphosis of Bombyx mori L.

Kim, Hak-Ryul,Yu, Jeong-Heui 한국곤충학회 1982 Korean journal of entomology Vol.12 No.1

누에 (Bombyx mori L.)의 변태기동안 표피의 RNA와 단백질의 농도 및 합성능을 Spectrophotometer(UV-360)와 Liquid Scintillation Counter (Beckman LS 100-C)로 측정 비교하였다. 표피의 RNA 농도는 전용초(고치형성기)에 최대에 달하였다가 그이후 감소하였으며 용화직후 다시 증가하였다. 또한 단백질 농도는 종령 유충말에 증가하였다가 그이후 감소하였으며 전용초에 최대에 달하였다가 다시 감소하였다. 혈림프내에 주입시킨 표피의 $^3$H-uridine의 방사능은 종령 유충말에서 전용말로 갈수록 감소하다가 다시 증가하여 용화후 1일에 최대에 달하였다. 또한 $^{14}$ C-leucine의 방사능은 종령 유충말과 전용말에 단백질농도의 증가양상과 일치한다. Concentration and synthetic activity of RNA and protein in epidermis during metamorphosis of Bombyx mori L. were determined by Spectrophotometer (UV-360) ana Liquid Scintillation Counter(Bookman LS 100-C). RNA concentration in epidermis reaches the peak at early prepupal stage (cocoon formation period) and falls down thereafter and then increase again just after pupation. Also, protein concentration rises at late last instar stage and decreases thereafter and then reaches the peak at late prepural stage folio wed by decrease. The radioactivity in epidermis of $\^$3/H-uriaine injected into haemocoel decreases during the transformation from late last instar larva to early prepupa and again increases and then reaches the peak 1 day after pupation. Also, radioactivity of $\^$14/C-leucine coincides with increasing pattern of protein at late instar larval and late prepupal stages.

Amylase in the Midgut during Metamorphosis of Pieris rapae L.

Kim, Hak-Ryul,Yoe, Sung-Moon 한국곤충학회 1982 Korean journal of entomology Vol.12 No.2

The activity, properties, and distribution of midgut amylase during metamorphosis in Pieris rapae L. are determined using Bookman UV Spectrophotometer and Hitachi 65 P-7 Automatic Ultracentrifuge. Optimal pH of midgut amylase is pH 8.5 in 5th instar larvae, pH 9.0 in prepupal and pupal stages. Also, midgut amylase activity increases in proportion to substrate concentration at relatively low conceal-rations until 0.6 % but scarcely at concentrations from 0.6 to 1.0 % from which it increases again constantly but slowly. Considerable amount of amylase was found in the midgut tissue of 5th instar larvae but in the prepupal and papal stages, most in the midgut conents. This suggested that amylase may be synthesized in the midgut during larval stage and then released into midgut lumen during prepupal stage. 배추흰나비(Pieris rapae L.)의 변태에 따른 중장내 amylase의 변화와 분포 및 성질을 조사 분석하였다. 중장 amylase의 최적 pH는 5령유충에서 8.5 전용기 및 용시기에서 9.0으로 각각 상이하게 나타났다. 또한 0.6%의 비교적 낮은 기질농도 까지는 amylase 활성도가 비례적으로 증가하나 0.6%에서 1.0% 까지는 거의 증가하지 않다가 이후 4.0% 까지 증가율은 낮으나 일정하게 증가한다. 5령유충의 중장조직에서는 상당히 많은 amylase가 발견되었으나 전용과 용시기에서는 중장조직에서 거의 발견되지 않고 중장내용물에서 발견되었다. 이러한 결과로 보아 amylase는 유충기 동안 중장조직에서 합성되었다가 전용기에 중장내강으로 방출되는 것 같다.

An immunological Study on Protein in Haemolymph of Pieris rapae L. during the Prepupal and Pupal stages

Kim, Hak-Ryul,Yoe, Sung-Moon 한국곤충학회 1980 Korean journal of entomology Vol.10 No.1

배추흰나비(Pieris rapae L.)의 전용 및 용시기에 따른 혈림프내 단백질을 비교하고 지방체내 단백질과의 상호관계를 규명하기 위하여 용화후 30분 된 혈림프를 토끼에 주사하여 항체를 얻은 다음 Immunodiffusion과 Immunoelectrophoresis 방법을 사용하였다. Immunodiffusion test에서는 총 8-9개의 precipitin line이 용초기에 걸쳐 혈림프에서 나타났는데 그 중 3개의 line은 전용기의 혈림프에서도 나타났다. 한편 용초기의 지방체에서는 4개의 line이 나타난 반면 전용기의 지방체에서는 2개의 line만이 나타났다. Immunoelectrophoresis test에서는 총 13개의 precipitin arc가 전용기와 용초기에 걸쳐 혈림프에서 나타난 반면 지방체에서는 전용기에 2개 용화직후에 5개의 arc가 나타났다. 이러한 결과는 적어도 4개의 혈단백질이 용화 전과 후에 지방체에서 합성되어 혈림프로 방출되었다는 것을 암시한다. In immunodiffusion test, a total of 8 to 9 precipitin lines are present in haemolymph throughout the early pupal stage while at the prepupal stage only three of them appear in haemolymph. On the while, in the fat body four lines occur at the early pupal stage while only two lines are present at prepupal stage. In immunoelectrophoresis test, a total of 13 precipitin arcs occur throughout prepupal and early pupal stages whereas fat body has two arcs at prepupal stage but five arcs immediately after pupation. These results suggest that at least four pupal haemolymph proteins are synthesized in the fat body before and after pupation and released into the haemolymph.

Free Paper Presentation : OS-66 ; Down-Regulated SIRT1 by Non-Steroidal Anti-Inflammatory Drugs Is to Inhibit TGF-β1-Induced Epithelial-Mesenchymal Transition and to Suppress Migration and Invasion in Lung Cancer

( Hak Ryul Kim ),( Ki Eun Hwang ),( Eun Taik Jeong ) 대한결핵 및 호흡기학회 2014 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.118 No.-

Background: Non-steroidal anti-inflammatory drugs (NSAIDs) has been reported to suppress lung cancer invasion and metastasis. However, the mechanism in lung cancer epithelial-mesenchymal transition (EMT) by NSAIDs is not clearly known. The class III deacetylase sirtuin 1 (SIRT1) possesses both pro- and anticarcinogenic properties. In this study, we investigated the role of NSAIDs as an inhibitor of TGF-β1-induced EMT, and the underlying mechanisms of suppressing lung cancer migration and invasion by celexib and sulindac. Methods: We evaluated the efficacy of celecoxib and sulindac in TGF-β1-induced EMT. EMT-related molecular alterations were detected by western blotting. Electric cell-substrate impedance sensing (ECIS) was used to evaluate the migration and invasion of A549 cells. The matrix metalloproteinase (MMP) gelatinolytic activity was determined by gelatin zymography, and protein expression was measured by western blotting. In addition, SIRT1 was knocked down or overexpressed to determine its role in preventing TGF-β1-induced EMT by celecoxib or sulindac. Results: Celecoxib was more effective in preventing TGF-β1-induced EMT, as compared with sulindac treatment, indicating upregulation of the epithelial marker, E-cadherin, and downregulation of N-cadherin, mesenchymal and transcription factors. Moreover, ECIS assay showed that celecoxib and sulindac could inhibit TGF-β1-enhanced migration and invasion of A549 cells. Increment in MMP-9 expression compared to that of MMP-2 following activation of TGF-β1 was also observed. However, treatment with celecoxib or sulindac inhibited MMP-9 expression. SIRT1 downregulation enhanced reverse of TGF- β1-induced EMT by celecoxib or sulindac. In contrast, SIRT1 upregulation had a relevant role in TGF-β1-induced EMT. Conclusions: Celecoxib and sulindac can inhibit TGF-β1-induced EMT and suppress lung cancer migration and invasion via down-regulation of SIRT-1. SIRT1 is positive regulator of TGF-β1-induced EMT and potential therapeutic target to reverse EMT and to prevent lung cancer progression.

The Development and Distribution of Haemolymph Proteins during the Fifth Instar Larval and Pupal Stages of Bombyx mori L.

Kim, Hak-Ryul,Yoe, Sung-Moon,Yoon, Won-Sick 한국곤충학회 1983 Korean journal of entomology Vol.13 No.1

누에(Bombyx mori)의 변태에 따른 체액내 단백질의 농도와 패턴 및 체액, 지방체, 중장, 타선내 단백질의 상호작용를 구명하기 위하여 Beckman UV spectrophotometer와 acrylamide gel electrophoresis 및 면역학적 방법을 사용하였다. 체액내 단백질의 패턴은 총 15개의 단백질 밴드가 분리되었는데 다소간의 정량적 변화를 보여 주어 종령기동안 높은 농도를 유지하다가 전용기 이후 급격히 감소한다. 체액내 단백질의 농도는 종령 9일의 유충에서 가장 높았으며 종령직후와 우화직전에는 상당히 낮게 나타난다. 타선 단백질과 동일한 지방체 단백질은 종령기와 우화직전에는 발견되지 않았으나 종령 6일부터 용화후 8일까지 1내지 3개의 단백질이 나타났다. 체액 단백질과 동일한 중장 단백질은 종령 유충기와 우화직전에는 발견되지 않았으나 전용기와 용시기동안 1 내지 3개의 단백질이 관찰되었다. 타선내 단백질은 전 시기를 통해 전혀 발견되지 않고 있어 누에의 견사형성에는 어떠한 체액 단백질도 직접 관여하지 않는 것 같다. The concentration and patterns of haemolymph proteins and relationships between proteins in haemolymph, fat body, midgut, and salivary gland during the metamorphosis of Bombyx mori L. were deter mined by Bookman UV spectrophotometer. acrylamide gel electrophoresis, and immunological method. There are a total of 15 protein bands in haemolymph throughout the whole stage, and these generally maintain high level during last larval stage, and then drastically decrease after prepupal stage, showing small quantitative changes. Protein concentration in haemolymph shows the highest peak at 9 day old last larvae, but represents very low level just after pupation and immediately before emergence. Blood proteins in the fat body were not detected at early last larvae and immediately before emergence but 1 to 3 proteins were observed from 6 day last larvae to 8 day pupae. Blood proteins in midgut were not detected at last larvae and immediately before emergence but 1 to 3 proteins observed during prepupal and pupal stages. There is no blood proteins in silk gland through-out the whle stage, suggesting no involvement of any haemolymph proteins in silk formation.