Supplementation of guanidinoacetic acid and rumen-protected methionine increased growth performance and meat quality of Tan lambs

Zhang Jian Hao,Li Hai Hai,Zhang Gui Jie,Zhang Ying Hui,Liu Bo,Huang Shuai,Guyader Jessie,Zhong Rong Zhen 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.10

Objective: Tan lambs (n = 36, 3 mo old, 19.1±0.53 kg) were used to assess effects of dietary guanidinoacetic acid (GAA) and rumen-protected methionine (RPM) on growth performance, carcass traits, meat quality, and serum parameters. Methods: Lambs were randomly assigned to three treatment groups, with 6 pens per group and 2 lambs per pen. Dietary treatments were: basal diet alone (I); basal diet supplemented with 0.08% GAA+0.06% RPM (II); and basal diet supplemented with 0.08% GAA+0.08% RPM (III). Diets were provided three times a day for 90 d. Intake per pen was recorded daily and individual lamb body weight (BW) was measured monthly. Carcass traits were measured after slaughter and meat quality at the end of the experiment, blood samples were taken on a subgroup of lambs for analysis of indicators mostly related to protein metabolism. Results: Final BW and average daily gain for the first and second month, and for the entire experiment were greater in Treatment II compared to Treatment I (p<0.05), whereas feed to gain ratio was lower (p<0.05). Treatment II had the optimal dressing percentage and net meat weight proportion, as well as crude protein and intramuscular fat concentrations in muscles. Treatment II improved meat quality, as indicated by the greater water holding capacity, pH after 45 min and 48 h, and lower shear force and cooking loss. Dietary supplementation of GAA and RPM also increased the meat color a* and b* values at 24 h. Finally, Treatment II increased total protein, and serum concentrations of albumin and creatinine, but decreased serum urea nitrogen concentrations, indicating improved protein efficiency. Conclusion: In this study, 0.08% GAA+0.06% RPM supplementation improved growth performance and meat quality of Tan lambs. Objective: Tan lambs (n = 36, 3 mo old, 19.1±0.53 kg) were used to assess effects of dietary guanidinoacetic acid (GAA) and rumen-protected methionine (RPM) on growth performance, carcass traits, meat quality, and serum parameters.Methods: Lambs were randomly assigned to three treatment groups, with 6 pens per group and 2 lambs per pen. Dietary treatments were: basal diet alone (I); basal diet supplemented with 0.08% GAA+0.06% RPM (II); and basal diet supplemented with 0.08% GAA+0.08% RPM (III). Diets were provided three times a day for 90 d. Intake per pen was recorded daily and individual lamb body weight (BW) was measured monthly. Carcass traits were measured after slaughter and meat quality at the end of the experiment, blood samples were taken on a subgroup of lambs for analysis of indicators mostly related to protein metabolism.Results: Final BW and average daily gain for the first and second month, and for the entire experiment were greater in Treatment II compared to Treatment I (p<0.05), whereas feed to gain ratio was lower (p<0.05). Treatment II had the optimal dressing percentage and net meat weight proportion, as well as crude protein and intramuscular fat concentrations in muscles. Treatment II improved meat quality, as indicated by the greater water holding capacity, pH after 45 min and 48 h, and lower shear force and cooking loss. Dietary supplementation of GAA and RPM also increased the meat color a* and b* values at 24 h. Finally, Treatment II increased total protein, and serum concentrations of albumin and creatinine, but decreased serum urea nitrogen concentrations, indicating improved protein efficiency.Conclusion: In this study, 0.08% GAA+0.06% RPM supplementation improved growth performance and meat quality of Tan lambs.

Self-Identity of the Passively Urbanized Group in the Process of Obtaining Urban Adaptability and Modernity: An Empirical Study on 561 Land-Displaced Peasants in Nanjing

Hai Bo Zhang,Xing Tong 전남대학교 지역개발연구소 2008 지역개발연구 Vol.40 No.2

Soluble expression, purification and the role of C-terminal glycine residues in scorpion toxin BmK AGP-SYPU2

( Rong Zhang ),( Yong Cui ),( Xi Zhang ),( Zhuo Yang ),( Yong Shan Zhao ),( Yong Bo Song ),( Chun Fu Wu ),( Jing Hai Zhang ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.12

The existence of glycine residues in long-chain scorpion toxins has been well documented. However, their role as analgesics has not been evaluated. To address this issue, we investigated the functional role of glycines in the C-terminal end of Chinese-scorpion toxin from Buthus martensii Karsch (BmK AGP-SYPU2) using site-directed mutagenesis and analgesic activity assays. Recombinant BmK AGP-SYPU2 and its mutants were efficiently expressed in E. coli and purified to homogeneity using immobilized metal ion affinity chromatography (IMAC) and cation exchange chromatography. The mouse-twisting test was used to detect the analgesic activity of BmK AGP-SYPU2 and its mutants. As a result, we identified glycines at the C-terminal end that, when altered, significantly affected analgesic activity. Also, Mut6566 was significantly decreased compared to BmK AGP-SYPU2. These data indicate that the glycines at the C-terminal end are important for the analgesic activity of BmK AGP-SYPU2. [BMB reports 2010; 43(12): 801-806]

Attenuation of Peripheral Regulatory T-Cell Suppression of Skin-Homing CD8+T Cells in Atopic Dermatitis

Bao-Xiang Zhang,Gang Ding,Jun-Cheng Lyu,Hai-Bo Liu,Dian-Cai Zhang,Dian-Cai Zhang,Xing-Jie Bi,Zhi-Wu Duan 연세대학교의과대학 2015 Yonsei medical journal Vol.56 No.1

Purpose: Cutaneous lymphocyte-associated antigen (CLA)-expressing CD8+T cells have been known to play an important role in the pathogenesis of atopic dermatitis(AD). However, the mechanisms underlying the loss of self-tolerance remainunclear. Regulatory T cells (Tregs) play a key role in the development of homeostasisin the immune system. We, therefore, hypothesized that a reduced ability of Tregs to inhibit autologous CD8+CLA+T cells might be underlying mechanism in AD. Materials and Methods: CD8+CLA+T cells and Tregs were obtained from the peripheral blood of AD patients and control volunteers. The frequenciesof CD8+CLA+T cells were evaluated. The proliferative responses of CD8+CLA+T cells were assessed by flow cytometry, and the levels of transforming growth factor-β1 (TGF-β1) and interleukin-10 (IL-10) in culture supernatants were detected by enzyme-linked immunosorbent assay. Results: Our results revealed higher frequency and increased expression of perforin and granzyme-B in peripheralCD8+CLA+T cells in AD, and lower inhibitory ability of Tregs on proliferation of CD8+CLA+T cells in AD. Meanwhile, the levels of TGF-β1 produced by Tregs were significantly lower in AD, and anti-TGF-β1 abolished such suppression. Conclusion: The attenuated inhibitory ability of Tregs on hyper-activated autologousCD8+CLA+T cells, mediated by TGF-β1, plays an important role in the pathogenesis of AD.

리그닌 함유 셀룰로오스 나노섬유로 강화된 폴리락틴산 나노복합재의 제조 및 분석

Hai Bo Sun,Xuan Wang,Li Ping Zhang 한국고분자학회 2014 폴리머 Vol.38 No.4

A chemo-mechanical method was used to prepare lignin-containing cellulose nanofibrils(L-CNF) from unbleached woodpulps dispersed uniformly in an organic solvent. L-CNF/PLA composites were obtained by solvent cast-ing method. The effects of L-CNF concentration on the composite performances were characterized by tensile test machine. contact angle machine, scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FTIR). The tensile test results indicated that the tensile strength and elongation-at-break increased by 50.6%and 31.8% compared with pure PLA. The contact angle of PLA composites decreased from 79.3° to 68.9°. The FTIR analysis suc- cessfully showed that L-CNF had formed intermolecular hydrogen bonding with PLA matrix.

Mechanical and Rheological Properties of Isotactic Polypropylene/Bacterial Cellulose Composites

Bo Wang,Hai-Rong Zhang,Chao Huang,Lian Xiong,Jun Luo,Xin-De Chen 한국고분자학회 2017 폴리머 Vol.41 No.3

To improve the mechanical performance of isotactic polypropylene (iPP), the iPP/bacterial cellulose (BC) composites were prepared. The tensile and impact strength of the composites were maximized (38.21MPa and 2.812 KJ/m²) when maleic anhydride grafted polypropylene (MAPP) content was 7 wt%, the tensile modulus was increased with the higher MAPP content and the maximum value was 1858.39 MPa. Compared with pure iPP, the tensile strength, impact strength and tensile modulus increased by 6.32 MPa, 0.75 KJ/m², and 279.68 MPa, respectively. Compared with the control group (the sample which added BC but no MAPP added), the tensile strength, impact strength and tensile modulus increased by 5.61 MPa, 0.692 KJ/m², and 11.24 MPa, respectively. Moreover, the elongation at break of the composites was decreased. Besides, the rheological results and SEM photographs indicated that with the addition of MAPP, the compatibility of the composites was improved greatly, which demonstrated the increase of the mechanical properties of the composites.

Correlation of UGT2B7 Polymorphism with Cardiotoxicity in Breast Cancer Patients Undergoing Epirubicin/Cyclophosphamide-Docetaxel Adjuvant Chemotherapy

Hai Li,Bo Hu,Zhe Guo,Xueqing Jiang,Xinyu Su,Xiaoyi Zhang 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.1

Purpose: The present study aimed to investigate correlations between uridine glucuronosyltransferase 2B7 (UGT2B7) -161 singlenucleotide polymorphism C to T (C>T) and the occurrence of cardiotoxicity in Chinese breast cancer (BC) patients undergoingepirubicin/cyclophosphamide-docetaxel (EC-D) adjuvant chemotherapy. Materials and Methods: 427 BC patients who had underwent surgery were consecutively enrolled in this prospective cohortstudy. All patients were scheduled to receive EC-D adjuvant chemotherapy regimen, and they were divided into UGT2B7 -161 CC(n=141), UGT2B7 -161 CT (n=196), and UGT2B7 -161 TT (n=90) groups according to their genotypes. Polymerase chain reactionwas performed for determination of UGT2B7 -161 genotypes. Cardiotoxicity was defined as an absolute decline in left ventricularejection fraction (LVEF) of at least 10% points from baseline to a value less than 53%, heart failure, acute coronary artery syndrome,or fatal arrhythmia. Results: LVEF values were lower at cycle (C) 4, C8, 3 months after chemotherapy (M3), M6, M9, and M12 compared to C0 (allp<0.001), in BC patients undergoing EC-D adjuvant chemotherapy. Cardiotoxicity was recorded for 4.2% of the overall populationand was lowest in the UGT2B7 -161 TT group (1.1%), compared to UGT2B7 -161 CT (3.1%) and UGT2B7 -161 CC (7.8%) group(p=0.026). Multivariate logistic regression revealed that UGT2B7 -161 T allele could independently predict a low occurrence ofcardiotoxicity in BC patients undergoing EC-D adjuvant chemotherapy (p=0.004). Conclusion: A UGT2B7 -161 T allele serves as a potential biomarker for predicting a low occurrence of cardiotoxicity in BC patientsundergoing EC-D adjuvant chemotherapy.

Cobalt Chloride Induces Necroptosis in Human Colon Cancer HT-29 Cells

Wang, Hai-Yu,Zhang, Bo Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6

Necroptosis, also known as "programmed necrosis", has emerged as a critical factor in a variety of pathological and physiological processes and is considered a cell type-specific tightly regulated process with mechanisms that may vary rather greatly due to the change of cell line. Here we used HT-29, a human colon cancer cell line, to establish a necroptosis model and elucidate associated mechanisms. We discovered that cobalt chloride, a reagent that could induce hypoxia-inducible $factor-1{\alpha}(HIF1{\alpha})$ expression and therefore mimic the hypoxic microenvironment of tumor tissue in some aspects induces necroptosis in HT-29 cells when caspase activity is compromised. On the other hand, apoptosis appears to be the predominant death form when caspases are functioning normally. HT-29 cells demonstrated significantly increased RIPK1, RIPK3 and MLKL expression in response to cobalt chloride plus z-VAD treatment, which was accompanied by drastically increased $IL1{\alpha}$ and IL6 expression, substantiating the notion that necrosis can induce profound immune reactions. The RIPK1 kinase inhibitor necrostatin-1 and the ROS scavenger NAC each could prevent necrosis in HT-29 cells and the efficiency was enhanced by combined treatment. Thus by building up a necroptosis model in human colon cancer cells, we uncovered that mechanically RIP kinases collaborate with ROS during necrosis promoted by cobalt chloride plus z-VAD, which leads to inflammation. Necroptosis may present a new target for therapeutic intervention in cancer cells that are resistant to apoptotic cell death.

Two new sesquiterpenoids from endophytic fungus J3 isolated from Mangrove Plant Ceriops tagal

Yan-Bo Zeng,Hai-Gang Gu,Wen-Jian Zuo,Li-Li Zhang,Hong-Jin Bai,Zhi-Kai Guo,Peter Proksch,Wen Li Mei,Hao Fu Dai 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.5

Two new sesquiterpenoids, named 2a-hydroxyxylaranolB (1) and 4b-hydroxyxylaranol B (2), togetherwith a known diterpenoid 3,4-seco-sonderianol (3) wereisolated from the fermentation of endophytic fungus J3 ofCeriops tagal. Their structures were elucidated based onspectroscopic methods including 1D and 2D NMR(HMQC, 1H-1H COSY and HMBC). All compounds wereevaluated for their cytotoxic activities by MTT method,and compound 3 exhibited cytotoxic activities againstK562, SGC-7901, and BEL-7402 cell lines.

A Novel Flow Diverter (Tubridge) for the Treatment of Recurrent Aneurysms: A Single-Center Experience

Yongxin Zhang,Qing-Hai Huang,Yibin Fang,Pengfei Yang,Yi Xu,Bo Hong,Jianmin Liu 대한영상의학회 2017 Korean Journal of Radiology Vol.18 No.5

Objective: The Tubridge flow diverter (FD) is a novel device aimed at reconstructing the parent artery and occluding complex aneurysms. Retreatment of recurrent aneurysms using the FD is challenging. We report our initial experience in the repair of aneurysm recurrence with the FD. Materials and Methods: A database was reviewed prospectively, and 8 patients with 8 recurrent aneurysms (mean size, 16.7 mm) were identified. Four aneurysms had previously ruptured. The previous aneurysm treatment consisted of coiling in 1 aneurysm and single-stent-assisted coiling in 7 aneurysms. The procedural complications and clinical and angiographic outcomes were analyzed. Results: Six aneurysms were treated by using a single Tubridge FD alone, while the remaining 2 were treated with FD + coiling. The immediate results of the 8 aneurysms were that they all showed incomplete occlusion. Neither major ischemic nor hemorrhagic complications occurred; however, 1 patient experienced a vasospasm. Follow-up angiographies were available for 7 aneurysms; the mean follow-up was 16.9 months (7–36 months). Five aneurysms were completely occluded, whereas 2 had a residual neck. Severe asymptomatic stenosis of 1 parent artery of a vertebral artery dissecting aneurysm was found. All visible branches covered by the FD were patent. All patients were clinically assessed as having attained a favorable outcome (modified Rankin Scale score ≤ 2) at discharge and follow-up. Conclusion: In selected patients, the Tubridge FD can provide a safe and efficient option for the retreatment of recurrent aneurysms. Nevertheless, attention should be paid to several technical points.