국내 분리 일본뇌염 바이러스 유전자에 대한 항체생성 연구(Ⅰ) : cloning of JEV viral genes

박찬,최우영,이호동,채수림,신은진,김인범,표재환,박근용,조해월 국립보건연구원 1999 국립보건원보 Vol.36 No.-

Syngas production by combined steam and CO₂ reforming of coke oven gas over highly sinter-stable La-promoted Ni/MgAl₂O₄ catalyst

Park, J.E.,Koo, K.Y.,Jung, U.H.,Lee, J.H.,Roh, H.S.,Yoon, W.L. Pergamon Press ; Elsevier Science Ltd 2015 International journal of hydrogen energy Vol.40 No.40

Highly sinter-stable 10 wt% Ni-xLa/MgAl<SUB>2</SUB>O<SUB>4</SUB> (x = 0-5 wt%) catalysts were prepared by co-impregnation for the combined steam and CO<SUB>2</SUB> reforming (CSCR) of coke oven gas (COG). The physicochemical properties of the catalysts were analyzed by BET, XRD, H<SUB>2</SUB> chemisorption, and H<SUB>2</SUB>-TPR. To compare the sinterstabilities, aging treatment was performed at 900 <SUP>o</SUP>C under H<SUB>2</SUB>:H<SUB>2</SUB>O:N<SUB>2</SUB> = 1:10:1.25 for 50 h. Although the Ni dispersions decrease and Ni crystallite sizes increase for all of catalysts after the aging treatment, the La-promoted catalysts had higher Ni dispersion and smaller crystallite sizes than the Ni/MgAl<SUB>2</SUB>O<SUB>4</SUB> catalyst owing to the enhancement of strong metal-support interactions. The catalytic test was carried out under CH<SUB>4</SUB>:H<SUB>2</SUB>O:CO<SUB>2</SUB>:H<SUB>2</SUB>:CO:N<SUB>2</SUB> = 1:1.2:0.4:2:0.3:0.3 at 900 <SUP>o</SUP>C and 5 atm for 40 h. The Ni-2.5La/MgAl<SUB>2</SUB>O<SUB>4</SUB> (aged) catalyst exhibited the highest activity and sinterstability owing to its high surface area and Ni dispersion. It was also confirmed that La promotion prevents the agglomeration of Ni particles through TEM.

Dynamics of H atom production from photodissociation of H₂O₂ at 205nm

Park, S.M.,Kang, C.M.,Kwon, C.H.,Kim, H.L. North Holland 2014 Chemical physics letters Vol.592 No.-

Photodissociation dynamics of H<SUB>2</SUB>O<SUB>2</SUB> at 205nm producing H atoms has been investigated by measuring laser-induced fluorescence spectra of H. From Doppler profile analyses, the average translational energy release was found to be 150.4+/-70.1kJ/mol with the anisotropy parameter, -0.35+/-0.05 for the characteristic perpendicular transition. Potential energy surfaces for the S<SUB>0</SUB>, S<SUB>1</SUB>, and T<SUB>1</SUB> electronic states along the dissociation coordinates were obtained employing density functional theory calculations at the B3LYP/aug-cc-pVTZ level. The dissociation of H from H<SUB>2</SUB>O<SUB>2</SUB> instantaneously takes place along the repulsive part of the triplet surface via rapid intersystem crossing from the initially excited S<SUB>1</SUB> state.

Inactivation of foodborne pathogens in powdered red pepper (Capsicum annuum L.) using combined UV-C irradiation and mild heat treatment

Cheon, H.L.,Shin, J.Y.,Park, K.H.,Chung, M.S.,Kang, D.H. Butterworths ; Taylor Francis ; Elsevier Science 2015 Food Control Vol.50 No.-

This study was performed to investigate the effectiveness of combined ultraviolet (UV-C) irradiation and mild heating as an alternative to conventional inactivation of foodborne pathogens, including Escherichia coli O157:H7 and Salmonella Typhimurium on powdered red pepper. A cocktail of three strains of E. coli O157:H7 ATCC 35150, ATCC 43889, ATCC 43890) and S. Typhimurium (ATCC 19585, ATCC 43971, DT 104) was inoculated onto powdered red pepper and then treated with UV-C irradiation and mild heat. A constant UV intensity (3.40 mW/cm<SUP>2</SUP>) of the emitting lamps was applied to samples for 5, and 10 min at 25, 35, 45, 55, and 65 <SUP>o</SUP>C, respectively. Also, quality change of powdered red pepper was measured in order to identify the efficiency of combined treatment. The reduction levels of E. coli O157:H7 and S. Typhimurium on powdered red pepper when treated with UV-C irradiation alone at 20.4 kJ/m<SUP>2</SUP> for 10 min was 0.22 and 0.29 log CFU/g, respectively. While, combined treatment with mild heating at 65 <SUP>o</SUP>C reduced the surviving numbers of each pathogens by 2.88 and 3.06 log CFU/g, respectively. Although the inactivation efficiency was influenced less by the UV-C radiation dose, the synergistic effect was observed with increasing temperature and UV-C radiation dose. CIE color value and extractable color value were not significantly (P > 0.05) different between non-treated and combination treated samples. The moisture and capsaicinoids contents showed significant (P < 0.05) differences when treated at 65 <SUP>o</SUP>C because of sample drying during heat treatment. Therefore, these results suggest that UV-C irradiation combined with mild heating can be utilized by the food industry in order to effectively inactivate E. coli O157:H7 and S. Typhimurium without incurring quality deterioration of powdered red pepper.

Enhanced delivery of liposomes to lung tumor through targeting interleukin-4 receptor on both tumor cells and tumor endothelial cells

Chi, L.,Na, M.H.,Jung, H.K.,Vadevoo, S.M.P.,Kim, C.W.,Padmanaban, G.,Park, T.I.,Park, J.Y.,Hwang, I.,Park, K.U.,Liang, F.,Lu, M.,Park, J.,Kim, I.S.,Lee, B.H. Elsevier Science Publishers 2015 Journal of controlled release Vol.209 No.-

A growing body of evidence suggests that pathological lesions express tissue-specific molecular targets or biomarkers within the tissue. Interleukin-4 receptor (IL-4R) is overexpressed in many types of cancer cells, including lung cancer. Here we investigated the properties of IL-4R-binding peptide-1 (IL4RPep-1), a CRKRLDRNC peptide, and its ability to target the delivery of liposomes to lung tumor. IL4RPep-1 preferentially bound to H226 lung tumor cells which express higher levers of IL-4R compared to H460 lung tumor cells which express less IL-4R. Mutational analysis revealed that C1, R2, and R4 residues of IL4RPep-1 were the key binding determinants. IL4RPep-1-labeled liposomes containing doxorubicin were more efficiently internalized in H226 cells and effectively delivered doxorubicin into the cells compared to unlabeled liposomes. In vivo fluorescence imaging of nude mice subcutaneously xenotransplanted with H226 tumor cells indicated that IL4RPep-1-labeled liposomes accumulate more efficiently in the tumor and inhibit tumor growth more effectively compared to unlabeled liposomes. Interestingly, expression of IL-4R was high in vascular endothelial cells of tumor, while little was detected in vascular endothelial cells of control organs including the liver. IL-4R expression in cultured human vascular endothelial cells was also up-regulated when activated by a pro-inflammatory cytokine tumor necrosis factor-α. Moreover, the up-regulation of IL-4R expression was observed in primary human lung cancer tissues. These results indicate that IL-4R-targeting nanocarriers may be a useful strategy to enhance drug delivery through the recognition of IL-4R in both tumor cells and tumor endothelial cells.

Effects of the novel angiotensin II receptor type I antagonist, fimasartan on myocardial ischemia/reperfusion injury

Han, J.,Park, S.J.,Thu, V.T.,Lee, S.R.,Long, L.T.,Kim, H.K.,Kim, N.,Park, S.W.,Jeon, E.S.,Kim, E.J.,Yoon, C.H.,Cho, G.Y.,Choi, D.J. Elsevier/North-Holland Biomedical Press 2013 INTERNATIONAL JOURNAL OF CARDIOLOGY Vol.168 No.3

Background: The aim of this study was to investigate the cardioprotective effect of fimasartan, a newly developed angiotensin II receptor type I blocker (ARB), against myocardial ischemia/reperfusion (I/R) injury and to identify the mechanism by which it reduces mitochondrial damage. Methods: Fimasartan was administered intravenously to Sprague-Dawley rats (3mg/kg), cardiomyocytes (50μM), and H9c2 cells (50μM) before ischemia or hypoxia. Myocardial infarction (MI), echocardiograms, DNA fragmentation, terminal deoxynucleotidyl transferase-mediated dUTP in situ nick-end labeling, immunoblotting, oxygen consumption, confocal microscopic appearance, and L-type Ca<SUP>2+</SUP> current (I<SUB>Ca,L</SUB>) were then assessed. Results: Fimasartan pretreatment remarkably reduced the rate of MI and improved cardiac performance well after I/R (n=9/group). Fimasartan also reduced apoptotic cell death both in vivo and in hypoxia/reoxygenation (H/R)-treated H9c2 cells (n=5~8/group). H/R-induced mitochondrial O<SUB>2</SUB><SUP>-</SUP> production and collapse of membrane potential were markedly attenuated in fimasartan-treated cardiomyocytes (n=4~6/group). Additionally, mitochondrial Ca<SUP>2+</SUP> overload during reoxygenation was suppressed by fimasartan (n=4~6/group), and this was found to be possibly related to the inhibition of I<SUB>Ca,L</SUB> and mitochondrial Ca<SUP>2+</SUP> uniporter. Furthermore, fimasartan pretreatment increased phosphorylations of Akt and glycogen synthase kinase-3β (n=5~7/group), decreased pro-apoptotic p53 levels, and increased anti-apoptotic Bcl-2 levels (n=4) during reperfusion. Conclusions: Fimasartan preconditioning has the potential to modulate Bcl-2 and suppress I/R-induced Ca<SUP>2+</SUP> overload by inhibiting I<SUB>Ca,L</SUB> and MCU. These beneficial effects could prevent the mitochondrial dysfunction and apoptosis accompanied by I/R.

만성 임파성 백혈병 1 예 보고와 문헌 고찰

박승광 ( S. K. Park ),유수웅 ( S. W. You ),이봉재 ( B. J. Lee ),성락훈 ( L. H. Sung ) 대한내과학회 1972 대한내과학회지 Vol.15 No.2

The purpose of this report is to illustrate the long course and rarity of C.L.L. in Korea. This is the first case of C.L.L. among 107 all leukemia during the past 10 years in National Medical Center. Variation in the incidence of C.L.L. are compared to oth

Biological evaluation of anti-influenza viral activity of semi-synthetic catechin derivatives

Song, J.M.,Park, K.D.,Lee, K.H.,Byun, Y.H.,Park, J.H.,Kim, S.H.,Kim, J.H.,Seong, B.L. Elsevier/North-Holland 2007 ANTIVIRAL RESEARCH Vol.76 No.2

Catechin derivatives with different alkyl chain length and aromatic ring substitutions at the 3-hydroxyl group were synthesized from epigallocatechin (EGC) and (+)-catechin (C) and their anti-influenza viral activity were evaluated in vitro and in ovo. Pronounced antiviral activity was observed for derivatives carrying moderate chain length (7-9 carbons) as compared to those with aromatic rings, whereas the 5'-hydroxyl group of the trihydroxy benzyl moiety did not significantly contribute to antiviral activity. The derivatives exerted inhibitory effects for all six influenza subtypes tested including three major types of currently circulating human influenza viruses (A/H1N1, A/H3N2 and B type), H2N2 and H9N2 avian influenza virus. The compounds strongly inhibited adsorption of the viruses on red blood cell (RBC). They also restricted the growth of avian influenza virus in ovo with minimum inhibition concentration (MIC) of 5-10μM far exceeding the neuraminidase (NA) inhibitor oseltamivir or M2 proton channel inhibitor amantadine. The antiviral activity appears to be mediated by interaction with hemagglutinin (HA)/viral membrane rendering HA less fusogenic at the initial stage of infection. The broad spectrum activity against various subtypes of influenza viruses may complement the limitations of current antivirals and contribute for managing potentially emerging influenza pandemic. The structure-activity data of catechin derivatives may usefully guideline future research endeavors for applying green tea catechins as alternative anti-viral agents.

Gibberellin secreting rhizobacterium, Pseudomonas putida H-2-3 modulates the hormonal and stress physiology of soybean to improve the plant growth under saline and drought conditions

Kang, S.M.,Radhakrishnan, R.,Khan, A.L.,Kim, M.J.,Park, J.M.,Kim, B.R.,Shin, D.H.,Lee, I.J. Gauthier-Villars ; Elsevier Science Ltd 2014 Vol. No.

The physiological changes in tolerant soybean plants under salt and drought stress conditions with Pseudomonas putida H-2-3 were investigated. A bacterial isolate H-2-3 was isolated from soil and identified as Pseudomonas putida H-2-3 by 16S rDNA sequences. The treatment of P. putida H-2-3 significantly increased the length, fresh and dry weight of shoot and chlorophyll content in gibberellins (GAs) deficient mutant Waito-c rice seedlings over the control, it might be the presence of GA<SUB>1</SUB>, GA<SUB>4,</SUB> GA<SUB>9</SUB> and GA<SUB>20.</SUB> The soybean plant growth was retarded in salt (120 mM sodium chloride) and drought (15% polyethylene glycol) stress conditions at 10 days treatments, while P. putida H-2-3 effectively enhanced the shoot length and fresh weight of plants suffered at salt and drought stress. The chlorophyll content was lower in abiotic stress conditions and bacterial inoculant P. putida H-2-3 mitigated the stress effects by an evidence of higher quantity of chlorophyll content in plants exposed to salt and drought. The stress hormonal analysis revealed that individual treatment of P. putida H-2-3, salt and drought significantly enhanced the abscisic acid and salicylic acid content than their control. P. putida H-2-3 applied to salt and drought stressed plants showed a lower level of abscisic acid and salicylic acid and a higher level of jasmonic acid content. Under stress condition induced by salt and drought in plants expressed higher level of total polyphenol, superoxide dismutase and radical scavenging activity and no significant changes in flavonoids. The bio-inoculant, P. putida H-2-3 modulated those antioxidants by declining superoxide dismutase, flavonoids and radical scavenging activity. P. putida H-2-3 induced tolerance against abiotic stress was confirmed by a reduction of Na content in abiotic stressed plants. The results suggest that P. putida H-2-3 application reprograms the chlorophyll, stress hormones and antioxidants expression in abiotic stress affected soybean plant and improves their growth under stress environment.

Effects of DME mixing on number density and size properties of soot particles in counterflow non-premixed ethylene flames

최재혁,B. C. Choi,S. M. Lee,S. H. CHUNG,K. S. Jung,W. L. Jeong,S. K. Choi,S. K. Park 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.5

In order to investigate the effect of DME mixing on the number density and size of soot particles, DME was mixed in a counter flownon-premixed ethylene flame with mixture ratios of 5%, 14% and 30%. A laser extinction/scattering technique has been adopted tomeasure the volume fraction, number density, and mean size of soot particles. The experimental results showed that the highest soot concentrationswere observed for flames with mixture ratios of 5% and 14%; however, for a mixture ratio of 30% the soot concentrationdecreased. Numerical results showed that the concentrations of propargyl radicals (C3H3) at the 5% and 14% ratios were higher thanthose measured in the ethylene-based flame, and the production of benzene (C6H6) in the 5% and 14% DME mixture flames was alsoincreased. This indicates the crucial role of propargyl in benzene ring formation. These reactions generally become stronger with increasedDME mixing, except for A1 + H2 → A1 + H (-R554) and n-C4H5 + C2H2 → A1 + H (R542). Therefore, it is indicated that addingDME to ethylene flames promotes benzene ring formation. Note that although the maximum C6H6 concentration is largest in the 30%DME mixing flame, the soot volume fraction is smaller than those for the 5% and 14% mixture ratios. This is because the local C6H6concentration decreases in the relatively low temperature region in the fuel side where soot growth occurs.