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Noh, Eun Woon,Lee, Jae Soon,Choi, Young Im,Lee, Hyo Shin,Bae, Eun Kyung,Lee, Ji Hee The Korean Society of Plant Biotechnology 2004 Plant molecular biology and biotechnology research Vol.6 No.1
The expression of a chimeric transgene (nos-npt II) has been examined in 9 years old transgenic poplars (Populus koreana x P. nigra) growing in a nursery. The expression of the gene in twenty six independentely transformed plants were examined by 1) enzyme (NPT II) assay, 2) RT-PCR, and 3) resistance to kanamycin. High NPT II activities in young leaves of all the transformed plants were found even without a selection pressure for antibiotics for 9 years. However, the activity varied with the positions of leaves in the stem in that young leaves showed higher activity than did mature tissues. When leaf segments were cultured in the presence of 150 mg/l kanamycin, only those from young leaves produced vigorously growing callus. However, as in the case of NPTII assay, the leaf segments from mature leaves did not form callus well on the media. RT-PCR with nptII specific primers also showed that amplification products were observed only when RNAs from young tissues were used. The total RNA gel showed that while RNA in young leaves are relatively stable and in a large quantity, those in old leaves were mostly degraded. All the above results suggest that the gene is transcriptionally active only in young tissue even though it is attached to a constituitive promoter. Therefore, the expression of foreign gene in poplar plants seemed to be affected by the metabolic state of the cells and thus vary greatly with the developmental stages and the age of tissue.
Eun Woon Noh 한국산림과학회 1992 한국산림과학회지 Vol.81 No.1
S-adenosylmethionine decarboxylase(SAMDC : EC 4.1.1.50) is a key regulatory enzyme in the polyamine biosynthetic pathway. Numerous studies have shown that the enzyme and polyamine levels are always correlated with cellular growth in plants. animals and bacteria. In order to gain more insight into the role of the pathway in plant cells, a human SAMDC cDNA was transferred totobacco (Nicotiana tabacaim) cells. Binary Agrobacterium tumefaciens vectors containing human SAMDC cDVA attached to 35s promoter of CAMV or chalcone synthase promoter of bean (Phaseolus vulgarzs) were constructed. Plants were transformed using Agrobacterium leaf disk cocultivation method. The transformed plants were selected on 120 ㎎/ℓ kanamycin. The presence of human SAMDC cDNA was confirmed by Polymerase Chain Reaction(.PCR) and subsequent Southern hybridization. The Northern hybridization showed that the cDNA was transcribed into two different sizes of mRNAs (1.7 and 2.1 kb). Western blotting using anti-rat SAMDC antibodies showed that proteins from some of the transformed plants reacted with the antibodies. Several of the transformed plants showed increased SAMDC activity. The polyamine assays showed that in the transformed plants, putrescine level was very low while spermidine content was 2-3 times higher than control plants. Spermine content re-mained unchanged. Most of the transformed plants frequently produced shoots even on callus inducing medium. Some plants produced smaller flowers and leaves when transferred to the greenhouse.
A Deletion in Fungal Ras Promoter in Two Korean Strains of Oak Mushroom (Lentinula edodes)
Noh, Eun-Woon,Lee, Jae-Soon,Park, Young-Im,Park, Won-Chull The Korean Society of Plant Pathology 2002 Plant Pathology Journal Vol.18 No.2
This study unexpectedly detected a deletion in the promoter region of ras gene in two Korean strains of oak mushrooms, Lentinula edodes (Berk.). Sequencing of the promoter regions revealed that one type consisting of two strains had a 113 bp deletion in the region. The pas promoter region of Korean strains differed by 16 bases from that of the Japanese strains. Between the two types of Korean strains, except for the deleted portion, only a single site appeared to be different.
Development of species specific RAPD markers using populus cpDNA
Jae Soon Lee(李載順)Eun Woon Noh(盧銀雲),Suk Sung Jang(張錫成),Suk Koo Lee(李錫求),Eui Rae Noh(盧義來),Don Koo Lee(李敦求) 한국육종학회 1994 한국육종학회지 Vol.26 No.4
Chloroplast DNAs(cpDNA) from eight different species of Populus(P. alba, P. glandulosa, P. alba×P. glandulosa, P. davidiana, P. maximowiczii, P. nigra×P. maximowiczii, P. nigra, and P. Koreana×P. nigra), Salix pseudo-lasiogyne and Nicotiana tabacum were compared by PCR amplification. The cpDNAs were amplified at relatively low annealing temperature so that many bands could be observed. Amplification of a spacer between 16S γRNA gene and 23S γRNA gene located in inverted repeats in cpDNA resulted in a major band in size of 2.3 Kb and several minor bands of various sizes. A band specific to Populus was found when the amplification was done at lower annealing temperature. Amplification with primers deduced from γpoC1 and γpoC2 gene produced the major target band as well as several additional (minor) bands that could be used for the differentiation of each Populus species. The minor bands appeared repeatedly even when different thermocycles were employed. With the amplification profile, it was possible to differentiate Populus species.
( Eun Jung Kim ),( Woo Chul Chung ),( Kang Moon Lee ),( Chang Nyol Paik ),( Sang Bae Kim ),( You Suk Oh ),( Yang Woon Lee ),( Sung Goo Kang ),( Seung June Noh ) 대한소화기학회 2013 Gut and Liver Vol.7 No.3
Background/Aims: We aim to evaluate the association be-tween promoter polymorphism of the clusters of differentia-tion 14 (CD14) gene and Helicobacter pylori-induced gastric mucosal inflammation in a healthy Korean population. Meth-ods: The study population consisted of 267 healthy subjects who visited our hospital for free nationwide gastric cancer screening. Promoter polymorphism at -260 C/T of the CD14 gene was determined by polymerase chain reaction and re-striction fragment length polymorphism analysis. The severity of gastric mucosal inflammation was estimated by a gastritis score based on the sum of the values of the grade and activ-ity of the gastritis. Expression of soluble CD14 (sCD14) was assessed by quantitative sandwich ELISA. Results: CD14 polymorphism was not associated with H. pylori infection. There were no significant differences in gastritis scores among the genotype subgroups, but subjects carrying the CD14 -260 CT/TT genotype had significantly higher sCD14 levels than those carrying the CC genotype. Subjects with the 260-T allele of the CD14 gene and H. pylori infection had significantly higher sCD14 levels than those with the same genotype but without infection. Conclusions: In individuals with the T allele at the -260 site of the promoter region of the CD14 gene, H. pylori infection accentuates gastric mucosal inflammation.
Comparison of oxidative stress markers in umbilical cord blood after vaginal and cesarean delivery
( Eun Ji Noh ),( Yoon Ha Kim ),( Moon Kyoung Cho ),( Jong Woon Kim ),( Jin Wook Kim ),( Yeung Ja Byun ),( Tae Bok Song ) 대한산부인과학회 2014 Obstetrics & Gynecology Science Vol.57 No.2
Objective The purpose of our study was to investigate the effect of the mode of delivery on the oxidant and antioxidant system in umbilical cord blood. Methods We performed gas analysis of umbilical venous blood and umbilical arterial blood immediately after delivery in 38 women; eighteen women had a vaginal delivery while 20 women delivered via cesarean section at over 37 weeks gestation. We examined lipid peroxide concentration by thiobarbituric acid reaction, protein carbonyl content by 2,4-dinitrophenylhydrazine reaction, and total antioxidant capacity by oxygen radical absorbance capacity assay. Results Lipid peroxide levels in umbilical venous blood were significantly higher in patients delivering by planned cesarean section (1.81 ± 0.06 nmol/mg protein) than those with vaginal delivery (1.24 ± 0.05 nmol/mg protein) ( P < 0.05). Antioxidant capacity in umbilical venous blood was significantly higher in patients delivering by planned cesarean section (119.70 ± 0.13 μM/μL) than those with a vaginal delivery (118.70 ± 0.29 μM/μL) ( P < 0.05). There was no significant difference in the carbonyl content of umbilical venous blood or in the lipid peroxide, carbonyl content, and total antioxidant capacity of umbilical arterial blood. Conclusion Lipid peroxidation levels and antioxidant capacity in umbilical venous blood were higher in patients delivering by planned cesarean section than those with a vaginal delivery. Therefore, we propose that both the mother and neonate are exposed to higher oxidative stress during cesarean section delivery.
Noh, Tae-Hwan,Lee, Jung-Woon,Lee, Young-A,Lee, Ji-Eun,Lee, Shim-Sung,Jung, Ok-Sang Korean Chemical Society 2008 Bulletin of the Korean Chemical Society Vol.29 No.3
Reaction of AgNO3 with triethanolaminetriisonicotinate (L) produces 1 D coordination polymer of [Ag3(L)2](NO3)3 and the same treatment of Cu(NO3)2 with L gives 1D coordination polymer of [Cu(L)2](NO3)2. The nonrigid triethanolaminetriisonicotinate acts as a m 3-bridged tridentate for [Ag3(L)2](NO3)3 and a m 2-bridged bidentate for [Cu(L)2](NO3)2 to produce unusual motifs. The NO3- anions can be smoothly exchanged by PF6- anions in an aqueous suspension without destruction of the skeletal structure.
Iodide management in formamidinium-lead-halide–based perovskite layers for efficient solar cells
Yang, Woon Seok,Park, Byung-Wook,Jung, Eui Hyuk,Jeon, Nam Joong,Kim, Young Chan,Lee, Dong Uk,Shin, Seong Sik,Seo, Jangwon,Kim, Eun Kyu,Noh, Jun Hong,Seok, Sang Il American Association for the Advancement of Scienc 2017 Science Vol.356 No.6345
<P>The formation of a dense and uniform thin layer on the substrates is crucial for the fabrication of high-performance perovskite solar cells (PSCs) containing formamidinium with multiple cations and mixed halide anions. The concentration of defect states, which reduce a cell's performance by decreasing the open-circuit voltage and short-circuit current density, needs to be as low as possible. We show that the introduction of additional iodide ions into the organic cation solution, which are used to form the perovskite layers through an intramolecular exchanging process, decreases the concentration of deep-level defects. The defect-engineered thin perovskite layers enable the fabrication of PSCs with a certified power conversion efficiency of 22.1% in small cells and 19.7% in 1-square-centimeter cells.</P>