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Magnetic Nanozyme-Linked Immunosorbent Assay for Ultrasensitive Influenza A Virus Detection
Oh, Sangjin,Kim, Jeonghyo,Tran, Van Tan,Lee, Dong Kyu,Ahmed, Syed Rahin,Hong, Jong Chul,Lee, Jaewook,Park, Enoch Y.,Lee, Jaebeom American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.15
<P>Rapid and sensitive detection of influenza virus is of soaring importance to prevent further spread of infections and adequate clinical treatment. Herein, an ultrasensitive colorimetric assay called magnetic nano(e)zyme-linked immunosorbent assay (MagLISA) is suggested, in which silica-shelled magnetic nanobeads (MagNBs) and gold nanoparticles are combined to monitor influenza A virus up to femtogram per milliliter concentration. Two essential strategies for ultrasensitive sensing are designed, i.e., facile target separation by MagNBs and signal amplification by the enzymelike activity of gold nanozymes (AuNZs). The enzymelike activity was experimentally and computationally evaluated, where the catalyticity of AuNZ was tremendously stronger than that of normal biological enzymes. In the spiked test, a straightforward linearity was presented in the range of 5.0 × 10<SUP>-15</SUP>-5.0 × 10<SUP>-6</SUP>g·mL<SUP>-1</SUP> in detecting the influenza virus A (New Caledonia/20/1999) (H1N1). The detection limit is up to 5.0 × 10<SUP>-12</SUP> g·mL<SUP>-1</SUP> only by human eyes, as well as up to 44.2 × 10<SUP>-15</SUP> g·mL<SUP>-1</SUP> by a microplate reader, which is the lowest record to monitor influenza virus using enzyme-linked immunosorbent assay-based technology as far as we know. Clinically isolated human serum samples were successfully observed at the detection limit of 2.6 PFU·mL<SUP>-1</SUP>. This novel MagLISA demonstrates, therefore, a robust sensing platform possessing the advances of fathomable sample separation, enrichment, ultrasensitive readout, and anti-interference ability may reduce the spread of influenza virus and provide immediate clinical treatment.</P> [FIG OMISSION]</BR>
Fast Drone Detection using SSD and YoloV3
Yew Ji Hao,Lee Koon Teck,Chua Ying Xiang,Enoch Jeevanraj,Sutthiphong Srigrarom 제어로봇시스템학회 2021 제어로봇시스템학회 국제학술대회 논문집 Vol.2021 No.10
This paper aims to introduce the method of detection of high-speed drones using both Single Shot Detector (SSD) and YOLOv3 (You Only Look Once)v3. After conducting experiments and obtaining footage of the fast-flying drones, the software and algorithms are being put to the test. In a motion detector, there are 3 main fundamentals - unmanned aerial vehicle (UAV) detection, UAV identification and tracking of the UAV, which will be introduced as a preliminary UAV detection system to spark of the use of other more advanced image recognition based detector. The alternative of using SSD and YOLOv3 will be the main discussion to target high-speed drones.
( Fangfang Sun ),( Sangjin Oh ),( Jeonghyo Kim ),( Tatsuya Kato ),( Hwa-jung Kim ),( Jaebeom Lee ),( Enoch Y. Park ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.8
In this study, silver nanoparticles (AgNPs) were synthesized by the citrate reduction process and, with the assistance of n-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, were successfully loaded with the macromolecular drug vancomycin (VAM) to form AgNP-VAM bioconjugates. The synthesized AgNPs, VAM, and AgNP-VAM conjugate were characterized by UV-visible spectroscopy, zeta potential analysis, confocal microscopy, and transmission electron microscopy. The effect of loading VAM onto AgNPs was investigated by testing the internalization of the bioconjugate into Mycobacterium smegmatis. After treatment with the AgNP-VAM conjugate, the bacterial cells showed a significant decrease in UV absorption, indicating that loading of the VAM on AgNPs had vastly improved the drug`s internalization compared with that of AgNPs. All the experimental assessments showed that, compared with free AgNPs and VAM, enhanced internalization had been successfully achieved with the AgNP-VAM conjugate, thus leading to significantly better delivery of the macromolecular drug into the M. smegmatis cell. The current research provides a new potential drug delivery system for the treatment of mycobacterial infections.
Ahmed, Syed Rahin,Oh, Sangjin,Baba, Rina,Zhou, Hongjian,Hwang, Sungu,Lee, Jaebeom,Park, Enoch Y. Springer US 2016 NANOSCALE RESEARCH LETTERS Vol.11 No.1
<P>The demand for biologically compatible and stable noble metal nanoparticles (NPs) has increased in recent years due to their inert nature and unique optical properties. In this article, we present 11 different synthetic methods for obtaining gold nanoparticles (Au NPs) through the use of common biological buffers. The results demonstrate that the sizes, shapes, and monodispersity of the NPs could be varied depending on the type of buffer used, as these buffers acted as both a reducing agent and a stabilizer in each synthesis. Theoretical simulations and electrochemical experiments were performed to understand the buffer-dependent variations of size and morphology exhibited by these Au NPs, which revealed that surface interactions and the electrostatic energy on the (111) surface of Au were the determining factors. The long-term stability of the synthesized NPs in buffer solution was also investigated. Most NPs synthesized using buffers showed a uniquely wide range of pH stability and excellent cell viability without the need for further modifications.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s11671-016-1290-3) contains supplementary material, which is available to authorized users.</P>
Photoluminescence enhancement of quantum dots on Ag nanoneedles
Ahmed, Syed Rahin,Cha, Hee Ryoung,Park, Jung Youn,Park, Enoch Y,Lee, Dongyun,Lee, Jaebeom Springer 2012 NANOSCALE RESEARCH LETTERS Vol.7 No.1
<P>Noble metal nanostructure allows us to tune optical and electrical properties, which has high utility for real-world application. We studied surface plasmon-induced emission of semiconductor quantum dots (QDs) on engineered metallic nanostructures. Highly passive organic ZnS-capped CdSe QDs were spin-coated on poly-(methyl methacrylate)-covered Ag films, which brought QDs near the metallic surface. We obtained the enhanced electromagnetic field and reduced fluorescence lifetimes from CdSe/ZnS QDs due to the strong coupling of emitter wave function with the Ag plasmon resonance. Observed changes include a six-fold increase in the fluorescence intensity and striking reduction in fluorescence lifetimes of CdSe/ZnS QDs on rough Ag nanoneedle compared to the case of smooth surfaces. The advantages of using those nanocomposites are expected for high-efficiency light-emitting diodes, platform fabrication of biological and environmental monitoring, and high-contrast imaging.</P>
( Yoon Suh Do ),( Seung-jae Myung ),( Sun-young Kwak ),( Soo Han Cho ),( Enoch Lee ),( Min Jeong Song ),( Chang Sik Yu ),( Yong Sik Yoon ),( Hye Kyung Lee ) 대한소화기기능성질환·운동학회 2015 Journal of Neurogastroenterology and Motility (JNM Vol.21 No.4
Background/Aims Chronic intestinal pseudo-obstruction (CIPO) is a disorder characterized by recurrent symptoms suggestive of obstruction such as abdominal pain, proximal distension with extremely suppressed motility in the absence of lumen-occluding lesion, whose etiology/ pathophysiology is poorly understood. In this study we investigated a functionally obstructive lesion that could underlie symptoms of CIPO. Methods We studied colons surgically removed from 13 patients exhibiting clinical/pathological features of pseudo-obstruction but were unresponsive to standard medical treatments. The colons were characterized morphologically, functionally and molecularly, which were compared between regions and to 28 region-matched controls obtained from colon cancer patients. Results The colons with pseudo-obstruction exhibited persistent luminal distension proximally, where the smooth muscle was hypertrophied with changes in the cell phenotypes. Distinct luminal narrowing was observed near the distal end of the dilated region, close to the splenic flexure, previously referred to as the “transition zone (TZ)” between the dilated and non-dilated loops. Circular muscles from the TZ responded less to depolarization and cholinergic stimulation, which was associated with downregulation of L-type calcium channel expression. Smooth muscle contractile protein was also downregulated. Myenteric ganglia and neuronal nitric oxide synthase (nNOS) positive cells were deficient, more severely in the TZ region. Interstitial cells of Cajal was relatively less affected. Conclusions The TZ may be the principal site of functional obstruction, leading to proximal distension and smooth muscle hypertrophy, in which partial nNOS depletion could play a key role. The neuromuscular abnormalities probably synergistically contributed to the extremely suppressed motility observed in the colonic pseudo-obstruction. (J Neurogastroenterol Motil 2015;21:560-570)