Kallmann syndrome with a Tyr113His <i>PROKR2</i> mutation

Ha, Jeong-Ha,Lee, Sara,Kim, Youngmoon,Moon, Ji In,Seo, Jongkwon,Jang, Ja-Hyun,Cho, Eun-Hae,Kim, Jung Min,Rhee, Byoung Doo,Ko, Kyung Soo,Yoo, Soo Jin,Won, Jong Chul Williams & Wilkins Co 2017 Medicine Vol.96 No.35

<P><B>Abstract</B></P><P><B>Rational:</B></P><P>Kallmann syndrome (KS) is a genetic gonadotropin-releasing hormone deficiency associated with hyposmia or anosmia and characterized by various modes of inheritance.</P><P><B>Patient concerns:</B></P><P>A 16-year-old male did not reach puberty and was associated with hypogonadotropic hypogonadism and anosmia. His magnetic resonance imaging of brain revealed the absence of the olfactory bulb.</P><P><B>Diagnosis:</B></P><P>His karyotype was 46 XY. Sanger sequencing of the <I>KAL1</I> gene revealed no mutations. Diagnostic exome sequencing identified a prokineticin-receptor 2 (<I>PROKR2</I>) gene variant, c.337T > C (p.Tyr113His), previously reported to be a pathogenic mutation; we confirmed the presence of the mutation via Sanger sequencing of the coding exons of <I>PROKR2</I>. His apparently unaffected mother and sister, but not his father, were heterozygous for the <I>PROKR2</I> Tyr113His mutation.</P><P><B>Lessons:</B></P><P>This work advances our understanding of the role played by PROKR signaling and the mode of inheritance of the gene in patients with KS.</P>

Clinical significance of Galectin-1 Expression in Cervical Cancer

( Ha Jeong Kim ),( Mun Kyeong Kim ),( Yoo Young Lee ),( Chel Hun Choi ),( Ha Jeong Kim ),( Tae Joong Kim ),( Jeong Won Lee ),( Byoung Gie Kim ),( Duk Soo Bae ) 대한산부인과학회 2011 대한산부인과학회 학술대회 Vol.97 No.-

The Effects of Fractions of Chick Embryo Extract on the Fusion of Cultured Chick Embryonic Myoblasts

Ha, Doo-Bong,Lee, Chung-Choo,Park, Young-Chul,Lim, Woon-Ki,Yoo, Byoung-Je The Korean Society for Integrative Biology 1985 동물학회지 Vol.28 No.3

In order to find factors which are essential for the differentiation of chick embryonic myoblasts in culture, chick embryo extract was fractionated by ammonium sulfate or/and Sephadex G-75, and the effects of each fraction on the proliferation and fusion of the myoblasts were examined. The results obtained were as follows: (1) High concentration of embryo extract in the culture medium enhanced the cell proliferation and delayed the fusion of myoblasts. (2) The Sephadex G-75 fractions of embryo extract having proteins of molecular weight between 40,000 and 22,000 enhanced the proliferation and fusion of myoblasts when added to culture media. (3) The fraction of embryo extract precipitated in $60\\sim95%$ saturated ammonium sulfate solution enhanced evidently both the proliferation and fusion of myoblasts. Elution of this effective fraction by Sephadex G-75 showed similar elution profile and effects on the myoblast differentiation as those observed by Sephadex G-75 chromatography of the whole embryo extract, suggesting that the Sephadex fractions and ammonium sulfate fractions contain the same factors that enhance the proliferation and fusion of myoblasts. 鷄胚의 筋原細胞의 증식과 融合에 미치는 계배 抽出液의 영향을 조사하였다. 1. 배양액내의 계배 추출액의 농도가 높을수록 근원세포의 증식은 촉진되었으며, 반면에 근원세포 융합은 지연되었다. 2. 계배 추출액의 단백질을 Sephadex G-75로 分劃하고, 각 분획을 근원세포의 배양액에 첨가한 결과 分子量 40,000과 22,000 dalton 사이의 분획이 근원세포의 증식과 융합을 촉진시켰다. 3. 계배 추출액의 단백질을 ammonium sulfate로 분획시켜 각 분획을 근원세포의 배양액에 첨가한 결과 70% 이상 포화 용액에서 침전하는 분획이 근원세포의 증식과 융합을 현저히 증가시켰다. 이 유효 분획을 Sephadex G-75로 재차 분획하여 각 분획의 효과를 조사한 결과 근원세포의 증식과 융합을 촉진시키는 분획이 계배 추출액을 Sephadex G-75로 분획하여 얻은 유효 분획과 거의 동일한 효과를 나타내었다.

오타고 운동과 요가 운동이 여성 노인의 보행, 우울 및 낙상 효능감에 미치는 효과 비교

유하나,정은정,이병희 대구대학교 특수교육재활과학연구소 2012 특수교육재활과학연구 Vol.51 No.2

The purpose of this study was to identify the effects of Otago exercise and Yoga exercise on gait, depression and fall efficacy of elderly women. Elderly women(n =21) were selected according to the conditions of the study and then divided into two groups: Otago exercise group(n =11) and Yoga exercise group(n=10). Otago exercise group received Otago exercise including strengthening, balance training for 60 minutes per day, 3 times per week over a 12 weeks. Yoga exercise group received hatha yoga exercise for the same time. Gait, depression and fall efficacy were measured to confirm the effects of intervention. After intervention, Otago group was significantly increased gait velocity(p =.000), cadence (p =.000), left step length(p =.002), stride length(left: p =.002, right: p =.007) and fall efficacy(p =.040). Yogagroup was sighnificantly increase gait velocity(p =.003), cadence(p =.027) and left step length(p =.048). The only significant difference found between the Otago group and Yoga group was in depression(p =.017). In conclusion, Otago exercise improves gait and fall efficacy of elderly women and Yoga exercise improves temporal gait function(gait velocity, cadence). These results suggest that the Otago exercise be more effective exercise on gait function and fall efficacy than the Yoga exercise in elderly women. 본 연구의 목적은 오타고 운동과 요가운동이 여성노인의 보행능력, 우울 및 낙상 효능감에 미치는 효과를 비교하고자 실시하였다. 서울 N지역에서 모집된 여성 노인 21명을 대상으로 무작위로 오타고 운동 11명과 요가 운동군 10명의 두 그룹으로 나누어 진행하였다. 오타고 운동군은 근력 강화 및 균형 훈련으로 구성하여 주 3회, 40분간 대상자의 수준에 맞춰 단계별로 실시하고 몸 풀기 운동과 마무리 운동을 10분씩 적용(총 60분)하였으며 요가운동군은 유연성 향상 및 스트레칭 효과가 있는 하타 요가를 선택하여 12주, 주당 3회, 회당 60분씩 실시하였다. 그 결과 오타고 운동군은 보행속도, 분속수, 왼쪽 보장, 양쪽 활보장 및 낙상 효능감에서 유의하게 향상되었고(p < .05), 요가 운동군은 보행속도, 분속수 왼쪽 보장에서 유의한 차이를 보였다(p < .05). 본 연구를 통하여 오타고 운동이 여성 노인의 보행 능력 및 낙상효능감에 효과가 있음을 확인하였고, 요가 운동은 보행변수 중 시간적 보행능력에서 효과가 있었음을 확인하였다. 앞으로 오타고 운동이 보행능력과 낙상예방의 효과를 보기 위 한 운동으로서 임상 및 노인시설에서 물리치료의 훈련 방법으로 적극 활용되어지길 기대하며, 더불어 요가운동도 우울과 같은 심리적인 요인뿐만 아니라 보행속도와 같은 시간적 보행 능력의 효과에 대한 재활 기초자료로 제공될 것이라 생각한다.

Synthesis of Muscle Proteins During the Differentiation of Cultured Chicken Pectoralis Muscle Cells

Ha, Doo-Bong,Im, Wook-Bin,Yoo, Byoung-Je The Korean Society for Integrative Biology 1981 동물학회지 Vol.24 No.4

근세포의 분화에 있어서의 근특이 단백질의 합성 순서를 구명하기 위하여 계배 근세포를 2$\\sim$9일간 배양하면서 단백질합성야상을 SDS-polyacrylamide 겔전기 영동법, 등전점초점2차원 전기영동법 및 방사자기법으로 분석하였다. Actin은 분화의 초기부터 활발히 합성되어 그 양이 다량으로 축적되나, myosin은 배양 3일째부터 대량 합성되기 시작하였다. Myosin의 대량합성시기는 배양 근원세포가 융합을 활발히 일으키는 시기와 거의 같았다. Myoglobin은 분화초기부터 서서히 합성축적되기 시작하여 배양 5일에서 최대치에 달하였다. Creatine phosphokinase는 배양 3일만에, 그리고 glyceraldehyde dehydrogenase는 6일만에 전기영동상에 검출되었다. Tropomyosin $\\alpha$와 $\\beta$, 그리고 troponin C는 분화초기부터 비교적 다량 합성되고 있었다. 젖산탈수소효소의 활성은 배양 2$\\sim$5일 사이에서 급격히 증가하고 이후 거의 변화가 없었다. 이 효소의 동위효소 조성은 초기 근원세포에서는 $H_4$와 $H_3M$형이 많으나 분화가 진행됨에 따라 $HM_3 와 M_4$형이 서서히 출현하였다. 그리고 배양 5일만에 5종의 동위효소가 모두 검출되었다.

Customized Asthma Control Test with Reflection on Sociocultural Differences

Yoo, Kwang-Ha,Jeong, Jae-Won,Yoon, Ho-Joo,Chang, Suk-Il,Moon, Hee-Bom,Choi, Byoung-Hwui,Kim, You-Young,Cho, Sang-Heon The Korean Academy of Medical Sciences 2010 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.25 No.8

<P>We evaluated the utility and feasibility of customizing Asthma Control Test (ACT) items to generate a Korean Asthma Control Test (KACT) specific for Korean patients. We surveyed 392 asthma patients with 19 items, selected to reflect the Korean sociocultural context. Guideline ratings were integrated with the evaluations of specialists (i.e., using both guide base rating together with specialist's rating), and items with the greatest discriminating validity were identified. Stepwise regression methods were used to select items. KACT scale scores showed significant differences between the asthma control ratings generated by integrating ratings (r=0.77, <I>P</I><0.001), by specialist's evaluations (r=0.54, <I>P</I><0.001), or by FEV<SUB>1</SUB> percent predicted (r=0.39, <I>P</I><0.001). Specialist's and guideline ratings detected 56% and 48.6% of patients with well-controlled asthma, respectively. However, the integrated ratings indicated that only 34.3% of the patients in the test sample were well controlled. The overall agreement between KACT and the integrated rating ranged from 45% to 78%, depending on the cut-off points used. It is possible to formulate a valid, useful country-specific diagnostic tool for the assessment of asthma patients based on the original ACT that reflect differences in sociocultural context.</P>

A comparison of uterine papillary serous, clear cell carcinomas, and grade 3 endometrioid corpus cancers using the revised FIGO staging system

( Ha Jeong Kim ),( Tae Joong Kim ),( Jin Young Park ),( Aera Yoon ),( Eun Ju Jo ),( Yoo Young Lee ),( Chel Hun Choi ),( Jeong Won Lee ),( Duk Sioo Bae ),( Byoung Gie Kim ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.98 No.-

This study was designed to compare the survival outcomes of patients with UPSC or CC to those of patients with G3EC according to the old and revised FIGO staging systems. We retrospectively reviewed all patients with endometrial cancer treated at a single institution between 1995 and 2009. Among the 647 patients with endometrial cancer, 51 with G3EC and 46 with UPSC/ CC histology were confirmed. FIGO 88 stage, FIGO 09 stage, and extrauterine metastasis were significantly different between the UPSC/CC and G3EC groups (p=0.002, p=0.041 and p=0.002, respectively). Restaging from the FIGO 88 to the FIGO 09 criteria increased the number of stage I cases by 10 (11.0%). Overall, 8 patients in the UPSC/CC group were down-staged to stage I, and 2 patients in G3EC were down-staged to stage I. The 5-year overall survival (OS) was 45.0% vs. 72.6% (p=0.038), and progression-free survival (PFS) was 58.7% vs. 77.8% (p=0.035) in the UPSC/CC and G3EC groups, respectively. In univariate analysis, a histologic type of UPSC/CC, a FIGO 09 stage of III-IV, and positivity for LVSI were negatively associated with PFS, as well as with OS. In multivariable analysis, UPSC/CC cell type, a FIGO 09 stage of III-IV, and LVSI were independent factors for OS. We observed that UPSC and CC histotypes might entail a worse survival outcome than G3EC in the 2009 FIGO staging system.

Basic, Research : Autophagy-related Marker LC3 can Predict Survival in Patient with Hepatocellular Carcinoma after Hepatic Resection

( Yoo Jin Lee ),( Yu Jin Ha ),( Yu Na Kang ),( Koo Jeong Kang ),( Woo Jin Chung ),( Jae Seok Hwang ),( Byoung Kuk Jang ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1

Patients and methods: Expression of autophagy related markers (LC3 and Beclin 1) and ER stress related markers (GRP78 and CHOP) were analyzed by immunohistochemistry on tissues from completely resected specimens of 190 HCC patients. Their influence on clinicopathologic features and prognosis were evaluated using Chi-square test and Kaplan-Meier analysis. Correlations of each proteins were determined by Spearman`s correlation analysis. Results: Expression of LC3 was not correlated with TNM, BCLC stage or Edmonson-Steiner grading, whereas, had correlation with longer overall survival (OS) (P=0.039) and longer disease free survival (DFS) (P=0.068) by Kaplan-Meier analysis. Multivariate analysis indicated that LC3 expression was significantly independent prognostic factors of OS (OR, 0.379; 95% CI, 0.192-0.748; P-value=0.005) and DFS (OR, 0.520; 95% CI, 0.299-0.843; P-value=0.009). Positive correlation was not observed in the expression of autophagy related markers with ER stress related markers. Conclusions: Our study showed that expression of LC3 was correlated with overall survival and disease free survival regardless of tumor staging or liver function state. These results suggest that expression and extent of LC3 might be a strong prognostic factor of HCC.

Thermally conductive polyamide 6/carbon filler composites based on a hybrid filler system

Ha, Sung Min,Kwon, O Hwan,Oh, Yu Gyeong,Kim, Yong Seok,Lee, Sung-Goo,Won, Jong Chan,Cho, Kwang Soo,Kim, Byoung Gak,Yoo, Youngjae TaylorFrancis 2015 SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS Vol.16 No.6

<P>We explored the use of a hybrid filler consisting of graphite nanoplatelets (GNPs) and single walled carbon nanotubes (SWCNTs) in a polyamide 6 (PA 6) matrix. The composites containing PA 6, powdered GNP, and SWCNT were melt-processed and the effect of filler content in the single filler and hybrid filler systems on the thermal conductivity of the composites was examined. The thermal diffusivities of the composites were measured by the standard laser flash method. Composites containing the hybrid filler system showed enhanced thermal conductivity with values as high as 8.8 W (m · K)<SUP>−1</SUP>, which is a 35-fold increase compared to the thermal conductivity of pure PA 6. Thermographic images of heat conduction and heat release behaviors were consistent with the thermal conductivity results, and showed rapid temperature jumps and drops, respectively, for the composites. A composite model based on the Lewis–Nielsen theory was developed to treat GNP and SWCNT as two separate types of fillers. Two approaches, the additive and multiplicative approaches, give rather good quantitative agreement between the predicted values of thermal conductivity and those measured experimentally.</P>

Risk and Predictors of Malignancy in asymptomatic postmenopausal women with sonographicallly thickened endometrium

( Ha Jeong Kim ),( Mun Kyeong Kim ),( Yoo Young Lee ),( Chel Hun Choi ),( Tae Joong Kim ),( Jeong Won Lee ),( Byoung Gie Kim ),( Duk Soo Bae ) 대한산부인과학회 2011 대한산부인과학회 학술대회 Vol.97 No.-