Cloning and expression analysis of the <i>Ccrboh</i> gene encoding respiratory burst oxidase in <i>Citrullus colocynthis</i> and grafting onto <i>Citrullus lanatus</i> (watermelon)

Si, Ying,Dane, Fenny,Rashotte, Aaron,Kang, Kwonkyoo,Singh, Narendra K. Oxford University Press 2010 Journal of experimental botany Vol.61 No.6

<P>A full-length drought-responsive gene <I>Ccrboh</I>, encoding the respiratory burst oxidase homologue (rboh), was cloned in <I>Citrullus colocynthis</I>, a very drought-tolerant cucurbit species. The robh protein, also named NADPH oxidase, is conserved in plants and animals, and functions in the production of reactive oxygen species (ROS). The <I>Ccrboh</I> gene accumulated in a tissue-specific pattern when <I>C</I>. <I>colocynthis</I> was treated with PEG, abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), or NaCl, while the homologous <I>rboh</I> gene did not show any change in <I>C</I>. <I>lanatus</I> var. <I>lanatus</I>, cultivated watermelon, during drought. Grafting experiments were conducted using <I>C</I>. <I>colocynthis</I> or <I>C</I>. <I>lanatus</I> as the rootstock or scion. Results showed that the rootstock significantly affects gene expression in the scion, and some signals might be transported from the root to the shoot. <I>Ccrboh</I> in <I>C</I>. <I>colocynthis</I> was found to function early during plant development, reaching high mRNA transcript levels 3 d after germination. The subcellular location of Ccrboh was investigated by transient expression of the 35S::Ccrboh::GFP fusion construct in protoplasts. The result confirmed that Ccrboh is a transmembrane protein. Our data suggest that <I>Ccrboh</I> might be functionally important during the acclimation of plants to stress and also in plant development. It holds great promise for improving drought tolerance of other cucurbit species.</P>

Supplementation of 100% Flesh Watermelon [Citrullus lanatus (Thunb.) Matsum. and Nakai] Juice Improves Swimming Performance in Rats.

Rasdin Ridwan,Hairil Rashmizal Abdul Razak,Mohd Ilham Adenan,Wan Mazlina Md Saad 한국식품영양과학회 2019 Preventive Nutrition and Food Science Vol.24 No.1

Nutritional intervention of fruit juice supplementation is able to maximize exercise performance. Watermelon [Citrullus lanatus (Thunb.) Matsum. and Nakai] contains high L-citrulline content and consumption of watermelon juice may promote ergogenic effects. The aim of the present study was to investigate the role of 100% flesh watermelon juice and 100% rind watermelon juice supplementation for 14 days on swimming performance in rats. Twenty four male Sprague-Dawley rats were randomly divided into four groups: Cx group of rats supplemented with filtered tap water (negative control), L-cit group of rats supplemented with L-citrulline (positive control), FR group of rats supplemented with 100% flesh watermelon juice, and RR group of rats supplemented with 100% rind watermelon juice. Each group was supplemented for 14 days ad libitum prior to swimming exercise protocol. The rats were performed swimming exercise for 3 days and swimming time until exhaustion was measured. Plasma samples were collected to measure lactate concentration, ammonia concentration, and nitric oxide production. Rats supplemented with 100% flesh watermelon juice demonstrated significantly prolonged of swimming time until exhaustion, reduction of lactate and ammonia concentrations, and increased of nitric oxide production compared to Cx and L-cit groups (P<0.05). These findings postulate that supplementation with 100% flesh watermelon juice improves endurance in swimming performance.

Identification of a candidate gene controlling semi-dwarfifi sm in watermelon, Citrullus lanatus, using a combination of genetic linkage mapping and QTL-seq

Youngwoo Cho,Siyoung Lee,Jiyeon Park,Seungahn Kwon,Girim Park,김회택,박영훈 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.3

Dwarfi sm in crops is a useful breeding trait. In this study, we aimed to identify a candidate gene controlling semi-dwarfi smin watermelon ( Citrullus lanatus ) using a combination of genetic mapping and quantitative trait loci (QTL)-seq. We evaluatedphenotypes using an F 2 and F 2:3 population derived from a cross between a “Bush Sugar Baby” (BSB, semi-dwarftype) and a PCL-J1 (normal type) cultivar. Results indicated that a single recessive gene confers semi-dwarfi sm in BSB. Weconstructed a genetic map using 180 F 2 plants and 336 single-nucleotide polymorphisms (SNPs), detected using genotypingby-sequencing, and mapped the semi-dwarfi sm locus, sdw-1 , between the SNP markers linked by 9.6 cM (0.99 Mb) on Chr. 9. QTL analysis pointed to the same genomic location for sdw-1 using single-marker analysis. Further, based on the QTL-seq,we identifi ed a signifi cant genomic region for sdw-1 that matched with the sdw-1- fl anking region in the genetic linkage map. This 1-Mb region was narrowed down by mapping three SNP markers developed from the QTL-seq data. The sdw-1 locuswas mapped to the 0.44-Mb genomic region, which harbored 13 genes. One of the 13 genes, ClCG09G018320 (ATP-bindingcassette (ABC) transporter B family member 19), showed the SNPs in its coding sequence, and cleaved amplifi ed APSmarkers developed from those SNPs co-segregated with sdw-1 in fi ne mapping using 620 F 2 plants. Our results implied thata frameshift mutation in the ABC transporter gene and the resulting alteration in auxin transportation are the most likely tobe responsible for semi-dwarfi sm in BSB.

Evaluating the Persistence of DNA from Decomposing Transgenic Watermelon Tissues in the Field

Bumkyu Lee,Ji-Young Park,박기웅,Chee Hark Harn,김환묵,김창기 한국식물학회 2010 Journal of Plant Biology Vol.54 No.5

To analyze the persistence of the 35S promoter,nos terminator, and hpt, we buried the leaves and rootstocks of transgenic watermelons (Citrullus lanatus (Thunb.)Matsum. & Nakai) in 10 cm of soil. Qualitative and quantitative PCR analyses showed that the amount of transgenes in leaf samples was greatly decreased, by 70%,after 1 month, and only 2.5% remained after 2 months. No transgenes were detected in the leaves after 3 months. For buried rootstock samples, transgenes also degraded quickly,but a very small amount was still detectable up to 3 months later. In our investigation of possible gene transfer from decomposing transgenic watermelon to soil bacteria, only the 35S promoter was detected. However, further examination using colony dot hybridization tests indicated that such a transfer did not occur.

Evaluating the Persistence of DNA from Decomposing Transgenic Watermelon Tissues in the Field

Lee, Bum-Kyu,Park, Ji-Young,Park, Kee-Woong,Harn, Chee-Hark,Kim, Hwan-Mook,Kim, Chang-Gi 한국식물학회 2010 Journal of Plant Biology Vol.53 No.5

To analyze the persistence of the 35S promoter, nos terminator, and hpt, we buried the leaves and rootstocks of transgenic watermelons (Citrullus lanatus (Thunb.) Matsum. & Nakai) in 10 cm of soil. Qualitative and quantitative PCR analyses showed that the amount of transgenes in leaf samples was greatly decreased, by 70%, after 1 month, and only 2.5% remained after 2 months. No transgenes were detected in the leaves after 3 months. For buried rootstock samples, transgenes also degraded quickly, but a very small amount was still detectable up to 3 months later. In our investigation of possible gene transfer from decomposing transgenic watermelon to soil bacteria, only the 35S promoter was detected. However, further examination using colony dot hybridization tests indicated that such a transfer did not occur.

Linkage Analysis of the Three Loci Determining Rind Color and Stripe Pattern in Watermelon

Yang, Hee-Bum,Park, Sung-woo,Park, Younghoon,Lee, Gung Pyo,Kang, Sun-Cheol,Kim, Yong Kwon Korean Society of Horticultural Science 2015 원예과학기술지 Vol.33 No.4

The rind phenotype of watermelon fruits is an important agronomic characteristic in the watermelon market. Inheritance and linkage analyses were performed for three rind-related traits that together determine the rind phenotype: foreground stripe pattern, rind background color, and depth of rind color. The inheritance of the foreground stripe pattern was analyzed using three different $F_2$ populations, showing that the striped pattern is dominant over the non-striped pattern. The inheritance analysis of the rind background color was performed using $F_2$ populations of the '10909' and '109905', and the depth of rind color was analyzed using $F_2$ populations of the '90509' and '109905'. Yellow color was found to be dominant over green color, and a deep color was dominant over the standard color. Linkage analysis of the three traits was conducted using three $F_2$ populations in which two traits were segregating. Each pair of traits was inherited independently, which demonstrated that the three traits are not linked. Therefore, we propose a three-locus model for the determination of rind phenotype, providing novel insight that rind phenotype is determined by the combination of three genetically independent loci.

Linkage Analysis of the Three Loci Determining Rind Color and Stripe Pattern in Watermelon

양희범,박성우,박영훈,이긍표,강순철,김용권 한국원예학회 2015 원예과학기술지 Vol.33 No.4

The rind phenotype of watermelon fruits is an important agronomic characteristic in the watermelon market. Inheritance and linkage analyses were performed for three rind-related traits that together determine the rind phenotype: foreground stripe pattern, rind background color, and depth of rind color. The inheritance of the foreground stripe pattern was analyzed using three different F2 populations, showing that the striped pattern is dominant over the non-striped pattern. The inheritance analysis of the rind background color was performed using F2 populations of the ‘10909’ and ‘109905’, and the depth of rind color was analyzed using F2 populations of the ‘90509’ and ‘109905’. Yellow color was found to be dominant over green color, and a deep color was dominant over the standard color. Linkage analysis of the three traits was conducted using three F2 populations in which two traits were segregating. Each pair of traits was inherited independently, which demonstrated that the three traits are not linked. Therefore, we propose a three-locus model for the determination of rind phenotype, providing novel insight that rind phenotype is determined by the combination of three genetically independent loci.

Linkage Analysis of the Three Loci Determining Rind Color and Stripe Pattern in Watermelon

Hee-Bum Yang,Sung-woo Park,Younghoon Park,Gung Pyo Lee,Sun-Cheol Kang,Yong Kwon Kim 한국원예학회 2015 원예과학기술지 Vol.33 No.4

The rind phenotype of watermelon fruits is an important agronomic characteristic in the watermelon market. Inheritance and linkage analyses were performed for three rind-related traits that together determine the rind phenotype: foreground stripe pattern, rind background color, and depth of rind color. The inheritance of the foreground stripe pattern was analyzed using three different F₂ populations, showing that the striped pattern is dominant over the non-striped pattern. The inheritance analysis of the rind background color was performed using F₂ populations of the ‘10909’ and ‘109905’, and the depth of rind color was analyzed using F₂ populations of the ‘90509’ and ‘109905’. Yellow color was found to be dominant over green color, and a deep color was dominant over the standard color. Linkage analysis of the three traits was conducted using three F₂ populations in which two traits were segregating. Each pair of traits was inherited independently, which demonstrated that the three traits are not linked. Therefore, we propose a three-locus model for the determination of rind phenotype, providing novel insight that rind phenotype is determined by the combination of three genetically independent loci.

Detection of Gene Flow from GM to non-GM Watermelon in a Field Trial

( Chang Gi Kim ),( Bum Kyu Lee ),( Dae In Kim ),( Ji Eun Park ),( Hyo Jeong Kim ),( Kee Woong Park ),( Hoon Bok Yi ),( Soon Chun Jeong ),( Won Kee Yoon ),( Chee Hark Harn ),( Hwan Mook Kim ) 한국식물학회 2008 Journal of Plant Biology Vol.51 No.1