Synergistic Effect of Natural Killer Cells and Bee Venom on Inhibition of NCI-H157 Cell Growth

Sung, Hee Jin,Song, Ho Sueb Korean AcupunctureMoxibustion Medicine Society 2016 대한침구의학회지 Vol.33 No.1

Objectives : This study examined the effects of Bee venom on apoptosis in NCI-H157 human lung cancer cells and for promoting the apoptosis effects of Natural killer cell. Methods : Bee venom and Natural killer-92 cells were cultured either separately from or together with NCI-H157 cells for 24 hours. To figure out whether Bee venom enhances the cytotoxic effect of Natural Killer-92 cells, a cell viability assay was conducted. To observe the changes in Death receptors, apoptotic regulatory proteins and Nuclear $Factor-{\kappa}B$, western blot analysis was conducted. To observe the effect of Bee venom through an extrinsic mechanism, a transfection assay was conducted. Results : 1. Natural killer-92 cells and Bee venom significantly inhibited the growth of NCI-H157 cells and co-culture had more inhibitory effect than the separate culture. 2. Expressions of Fas, DR3, DR6, Bax, caspase-3, caspase-8, cleaved caspase-3, cleaved caspase-8 were increased, and expressions of Bcl-2 and cIAP were decreased. More efficacy was observed in co-culture than in separate culture. 3. Nuclear $Factor-{\kappa}B$ activation was clearly decreased. And co-culture showed much less activation than separate culture. 4. As a result of treatment for DR-siRNA, the reduced cell viability of NCI-H157 cells and the activity of Nuclear $Factor-{\kappa}B$ were increased. With this, it can be seen that Bee venom and Natural killer-92 cells have an effect on the cancer cells through the extrinsic mechanism. Conclusion : Bee venom is effective in inhibiting the growth of human lung cancer cells. Furthermore Bee venom effectively enhances the functions of Natural killer cells.

Synergistic Effect of Natural Killer Cells and Bee Venom on Inhibition of NCI-H157 Cell Growth

성희진,송호섭 대한침구의학회 2016 대한침구의학회지 Vol.29 No.6

Objectives : This study examined the effects of Bee venom on apoptosis in NCI-H157 human lung cancer cells and for promoting the apoptosis effects of Natural killer cell. Methods: Bee venom and Natural killer-92 cells were cultured either separately from or together with NCI-H157 cells for 24 hours. To figure out whether Bee venom enhances the cytotoxic ef- fect of Natural Killer-92 cells, a cell viability assay was conducted. To observe the changes in Death receptors, apoptotic regulatory proteins and Nuclear factor-κB, western blot analysis was conducted. To observe the effect of Bee venom through an extrinsic mechanism, a trans- fection assay was conducted. Results: 1. Natural killer-92 cells and Bee venom significantly inhibited the growth of NCI-H157 cells and co-culture had more inhibitory effect than the separate culture. 2. Expressions of Fas, DR3, DR6, Bax, caspase-3, caspase-8, cleaved caspase-3, cleaved caspase-8 were increased, and expressions of Bcl-2 and cIAP were decreased. More ef- ficacy was observed in co-culture than in separate culture. 3. Nuclear Factor-κB activation was clearly decreased. And co-culture showed much less activation than separate culture. 4. As a result of treatment for DR-siRNA, the reduced cell viability of NCI-H157 cells and the activity of Nuclear factor-κB were increased. With this, it can be seen that Bee venom and Natural killer-92 cells have an effect on the cancer cells through the extrinsic mechanism. Conclusion : Bee venom is effective in inhibiting the growth of human lung cancer cells. Fur- thermore Bee venom effectively enhances the functions of Natural killer cells.

Synergistic Effect of Natural Killer Cells and Bee Venom on Inhibition of NCI-H157 Cell Growth

Sung, Hee Jin,Song, Ho Sueb Korean AcupunctureMoxibustion Medicine Society 2016 대한침구의학회지 Vol.29 No.6

Objectives : This study examined the effects of Bee venom on apoptosis in NCI-H157 human lung cancer cells and for promoting the apoptosis effects of Natural killer cell. Methods : Bee venom and Natural killer-92 cells were cultured either separately from or together with NCI-H157 cells for 24 hours. To figure out whether Bee venom enhances the cytotoxic effect of Natural Killer-92 cells, a cell viability assay was conducted. To observe the changes in Death receptors, apoptotic regulatory proteins and Nuclear $Factor-{\kappa}B$, western blot analysis was conducted. To observe the effect of Bee venom through an extrinsic mechanism, a transfection assay was conducted. Results : 1. Natural killer-92 cells and Bee venom significantly inhibited the growth of NCI-H157 cells and co-culture had more inhibitory effect than the separate culture. 2. Expressions of Fas, DR3, DR6, Bax, caspase-3, caspase-8, cleaved caspase-3, cleaved caspase-8 were increased, and expressions of Bcl-2 and cIAP were decreased. More efficacy was observed in co-culture than in separate culture. 3. Nuclear $Factor-{\kappa}B$ activation was clearly decreased. And co-culture showed much less activation than separate culture. 4. As a result of treatment for DR-siRNA, the reduced cell viability of NCI-H157 cells and the activity of Nuclear $Factor-{\kappa}B$ were increased. With this, it can be seen that Bee venom and Natural killer-92 cells have an effect on the cancer cells through the extrinsic mechanism. Conclusion : Bee venom is effective in inhibiting the growth of human lung cancer cells. Furthermore Bee venom effectively enhances the functions of Natural killer cells.

치료중인 유방암 환자의 신체적 증상과 자연살해세포 활성도의 관계

채영란 대한기초간호자연과학회 2002 Journal of korean biological nursing science Vol.4 No.2

The purpose of this study was to identify the relationship of symptorn discress and natural killer cell cytotoxicity in breast cancer patients who had been radiation therapy and/or chemotherapy after surgery. Symptom distress measured by modified Lee's(1994) physical symptom questionnaire. For measuring the natural killer cell cytotoxic activity, 8ml to 10ml blood was collected from the subjects. Mononuclear cell was isolated by centrifuge of the blood and cultured by putting Cr^(51) , and reacted with target cell, K562 cell. Amount of Cr^(51) was measured, and %lysis was calculated. The results were as follows. 1) Symptom distress score was 42.18, which is moderate symptom distress. 2) Natural killer cell cytotoxic activities were 42.18%lysis(effector : target cell ratio=100 : 1) and 28.05%lysis(effector : target cell ratio=50 : 1). 3) Correlation coefficients of symptom distress and natural killer cell cytotoxic activity were -.134~-.461. Though significant correlation was not found between total score of symptom distress and natural killer cell cytotoxic activity, 3('pain' 'feel hot on radiation site' and 'difficulty in breathing') of 19 symptom distress items and natural killer cell cytotoxic activity showed significant negative correlation(p<.05). These findings suggest that 1) breast cancer patients who had been radiation therapy and/or chemotherapy after surgery have moderate symptom distress and decreased natural killer cell cytotoxic activity. 2) The symptom distress was not related to natural killer cell cytotoxic activity.

Distribution of Lymphocyte Subpopulation and Natural Cytotoxicity of Peripheral Blood from Colon Cancer Patient

Park, Il Young,Park, Jang Sang,Chang, Suk Kyun,Lee, Jae Hak CATHOLIC MEDICAL CENTER 1990 Bulletin of the Clinical Research Institute Vol.18 No.1

Depression of general immune reactivity has been well documented in patients with colorectal cancer as well as in patient with other types of solid tumors. The main effector of immune surveillance against cancer have been considered to be sensitized T lymphocyte and activated macrophages. Recently there has been increasing recognition that natural cell-mediated cytotoxicity is potentially an important antitumor mechanism especially in recurrence, metastasis and prognosis. Authors investigated to study the relation between subpopulation, stimulated or non-stimulated natural cytotoxicity of lymphocyte and stage of disease, serum CEA levels, or differentiation of cancer. The results were as follows; 1. The peripheral blood lymphocyte count was significantly decreased only in group 4 as compared with control. 2. The natural cytotoxicity against K_562 cells were significantly decreased in group 2 and group 4 than that of control. But except for group 3, there were no significant differences in natural cytotoxicity against SBA cells. 3. The natural cytotoxieity against K_562 cells of interleukin-2 stimulated lymphocyte was significantly increased than that of non-stimulated lymphocytes in both of control and stage Ⅳ patients group. 4. The distributions of lymphocyte subpopulation (CD_4, CD_8, CD_16) were slightly decreased in group 3 and decrease of CD_4, CD_16 with increase of CD_8 in group 4 without statistical significancy. But in group 3, CD_4 cells were significantly increased than that of control and CD_8 cells were significantly increased as compared with control. 5. The ratio of CD_4 to CD_8 was significantly decreased in group 3 than that of control, but no significant differences in group 2 and 4. 6. The serum CEA was positive (>5 ng/ml) in 43% of patients and the level was increased with advance of diseases. But there were no correlation between serum CEA level, tumor cell differentiation and natural cytotoxicity, CD_4 to CD_8 ratio or K_562 cytotoxicity to CD_15 cells. With above results, authors insist that the natural cytotoxicity by natural killer (NK) cells is decreased but not by cytotoxic T-cell in colon cancer patients and these cytotoxic activities can be enhenced by interleukin-2 stimulation. The NK cells was slightly increased in all stage of disease, but the decrease of helper T cell and increase of suppressor T-cell were significant in patients with lymph node metastasis.

정신분열증과 주요 우울장애에서 Natural Killer Cell 수와 백분율 변화NK cell

윤장봉,이재우,박두병 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.5

The purpose of this study was to compare the number and percentage of natural killer cell in schizophrenic and major depressive patients with those in normal healthy subjects 15 Schizophrenic patients, 15 major depressive patients and 16 healthy subjects were included in this study There was no statistical difference in the age and sex between patients and control subjects. All of these patients were drug-free for at lest two weeks before this study. The results of this study were as follows : 1) In schizophrenic patients, the number and percentage of natural killer cell of the group with below-mean score of general psychopathology were significantly lower than those of the group with above-mean score(p<.05). The number but not the percentage of natural killer cell of the group with below-mean BPRS score was significantly lower than that of the group with above-mean BPRS score(p<.05). 2) The number of natural killer cell showed statistically negative correlation with generalpsychopathology score or BPRS score(p<0.05). In conclusion, these findings suggest that the number and percentage of natural killer cell is not significantly changed in schizophrenia and major depressive disorder, but in schizophrenic patients, severe psychopathology in the rating scales is associated with suppression of the number and percentage of natural killer cell.

운동 프로그램이 유방암 환자의 자연살해세포 활성에 미치는 효과

채영란,최명애,김미정 대한기초간호자연과학회 2002 Journal of korean biological nursing science Vol.4 No.2

The purpose of this study was to determine the effect of exercise program on natural killer cell cytotoxic activity(NKCA) in breast cancer patients who had been radiation therapy after surgery. The subjects in the experimental group consisted of 11 breast cancer patients, while the subjects in the control group consisted of 15. Subjects in the experimental group participated in exercise program for 8 weeks. Exercise program consisted of shoulder stretching, arm weight training and treadmill walking exercise. They started to exercise on treadmill for 20 minutes per day, 3 times a week 40% of maximum heart rate, and increased intensity and duration of exercise so that they were running 30 minutes/day at 60% of maximum heart rate from the 3rd week to the 8th week. Natural killer cell cytotoxic activity were determined before and after tht: exercise program. For measuring the natural killer cell cytotoxic activity, 8ml to 10ml blood was collected from the subjects. Mononuclear cell was isolated by centrifuge of the blood and cultured by putting Cr^(51) , and reacted with target cell, K562 cell. Baseline demographic and medical data were compared between groups with the Fisher's exact test and Mann-Whitney U test. For effects of the exercise program, repeated measures ANOVA was used. The result was as follows ; Natural killer cell cytotoxic activity(NKCA) in experimental group comparing with control group significantly increased after the exercise program in case of effector cell : target cell ratio is 100 : 1(p<0.05). The above result suggest that the exercise program for breast cancer patients undergoing radiation therapy after breast surgery may increase the natural killer cell cytotoxic activity.

인체신세포암에서 자연살해세포의 활성화

류현열,Eschenbach, Andrew C. von 고신대학교 의학부 1995 高神大學校 醫學部 論文集 Vol.10 No.2

Natural killer (NK) cells that had infilterated renal cell carcinoma(RCC) proliferated vigorously in culture which activated interleukin-2(IL-2) and lysed autologous tumor cells. We studied the susceptibility of RCC cells to NK-cell lysis and their ability to stimulate proliferation and function of NK cells. Cells from primary culture of RCC(p-RCC cells) were significantly more susceptible to lysis mediated by human NK3.3 clones than were cells from primary culture of metastatic melanomas. RCC cells clones was also susceptible to lysis by NK3.3 clones and IL-2 activated peripheral blood lymphocytes(PBLs). Incubation of NK3.3 clones with p-RCC cells in the absence of IL-2 induced proliferation of NK3.3 clones, whereas incubation with cells from primary culture of metastatic melanomas, K562 cells tested did not. The p-RCC cells from earlier passages were more potent inducers of NK-cell proliferation than were those from older passages. Cell-free culture supernatants of p-RCC cells with or without NK3.3 clones failed to induce NK-cell proliferation. Incubation of NK cells purified from PBLs with p-RCC cells induced higher proliferation of the NK cells only in the presence of IL-2, whereas incubation with cells from primary culture of metastatic melanomas did not. In summary, these results suggest that RCC cells are able to activate NK cells, potentially through cell-to-cell interaction.

Role of Interleukin(IL)-6 in NK Activity to Hypoxic-Induced Highly Invasive Hepatocellular Carcinoma(HCC) Cells

( Hwan Hee Lee ),( Hyojung Kang ),( Hyosun Cho ) 한국미생물생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.7

Natural killer (NK) cell dysfunctions against hepatocellular carcinoma (HCC) in a hypoxic environment. Many solid tumors are present in a hypoxic condition, which changes the effector function of various immune cells. The transcription of hypoxic-inducible factors (HIFs) in cancer cells make it possible to adapt to their hypoxic environment and to escape the immune surveillance of NK cells. Recently, the correlation between the transcription of HIF-1α and pro-inflammatory cytokines has been reported. Interleukin (IL)-6 is higher in cancers with a highly invasive ability, and is closely related to the metastasis of cancers. This study showed that the expression of HIF-1α in HCC cells was associated with the presence of IL-6 in the environment of HCC-NK cells. Blocking of IL-6 by antibody in the HCC-NK interaction changed the production of several cytokines including TGF-β, IL-1, IL-18 and IL-21. Interestingly, in a co-culture of HIF-1α-expressed HCC cells and NK cells, blocking of IL-6 increased the production of IL-21 in their supernatants. In addition, the absence of IL-6 significantly enhanced the cytotoxic ability and the expression of the activating receptors (NKG2D, NKp44, and NKG2C) in NK cells to HIF-1α-expressed HCC cells. These effects might be made by the decreased expression of HIF-1α in HCC cells through the inhibited phosphorylation of STAT3. In conclusion, the absence of IL- 6 in the interaction of HIF-1α-expressed HCC cells and NK cells could enhance the antitumor activity of NK cells to HCC cells.

Mitomycin-C, Cisplatin, Vinblastine이 마우스 자연살해세포 활성에 미치는 영향

강헌중,김광혁,류현열,박건영 대한암예방학회 2000 Journal of cancer prevention Vol.5 No.3