Antioxidant Activities of Fractions from Sedum sarmentosum

Inshik Park,Choon Young Kim,Min-Young Lee 한국식품영양과학회 2006 Preventive Nutrition and Food Science Vol.11 No.2

This study was conducted to evaluate the antioxidant activity of each fraction from Sedum sarmentosum. Antioxidant activity of each fraction was measured using the DPPH radical assay, the ferric thiocyanate (FTC) method, and thiobarbituric acid (TBA) method. The antioxidant activities were then compared with that of BHT (synthetic antioxidant). The ethyl acetate and butanol fractions were found to have significant DPPH radical scavenging activity, with scavenging potencies showing 90.61% and 87.02%, respectively. Total phenolic compound contents, determined according to the Folin-Denis method, were found to be in the order of ethyl acetate>butanol>ethanol>chloroform>aqueous fraction. From the results, we have been able to establish a positive correlation between the antioxidant activity and the total phenolic compound content of the sample. The antioxidant activity in a linoleic acid system was measured using the ferric thiocyanate (FTC) method and thiobarbituric acid (TBA) method. The ethyl acetate fraction had the highest antioxidant activity among the tested fractions. On the basis of these results, the ethyl acetate fraction provided equivalent or higher antioxidant activity as compared to BHT. These results suggest that Sedum sarmentosum is a potentially useful antioxidant for foods, cosmetics, and medicine.

Antioxidant Activities of Fractions from Sedum sarmentosum

Choon Young Kim,Min-Young Lee,Inshik Park 한국식품영양과학회 2006 Preventive Nutrition and Food Science Vol.11 No.1

This study was conducted to evaluate the antioxidant activity of each fraction from Sedum sarmentosum. Antioxidant activity of each fraction was measured using the DPPH radical assay, the ferric thiocyanate (FTC) method, and thiobarbituric acid (TBA) method. The antioxidant activities were then compared with that of BHT (synthetic antioxidant). The ethyl acetate and butanol fractions were found to have significant DPPH radical scavenging activity, with scavenging potencies showing 90.61% and 87.02%, respectively. Total phenolic compound contents, determined according to the Folin-Denis method, were found to be in the order of ethyl acetate>butanol>ethanol>chloroform>aqueous fraction. From the results, we have been able to establish a positive correlation between the antioxidant activity and the total phenolic compound content of the sample. The antioxidant activity in a linoleic acid system was measured using the ferric thiocyanate (FTC) method and thiobarbituric acid (TBA) method. The ethyl acetate fraction had the highest antioxidant activity among the tested fractions. On the basis of these results, the ethyl acetate fraction provided equivalent or higher antioxidant activity as compared to BHT. These results suggest that Sedum sarmentosum is a potentially useful antioxidant for foods, cosmetics, and medicine.

Antioxidant Activities of Fractions from Sedum sarmentosum

Kim, Choon-Young,Lee, Min-Young,Park, In-Shik The Korean Society of Food Science and Nutrition 2006 Preventive Nutrition and Food Science Vol.11 No.1

This study was conducted to evaluate the antioxidant activity of each fraction from Sedum sarmentosum. Antioxidant activity of each fraction was measured using the DPPH radical assay, the ferric thiocyanate (FTC) method, and thiobarbituric acid (TBA) method. The antioxidant activities were then compared with that of BHT(synthetic antioxidant). The ethyl acetate and butanol fractions were found to have significant DPPH radical scavenging activity, with scavenging potencies showing 90.61 % and 87.02%, respectively. Total phenolic compound contents, determined according to the Folin-Denis method, were found to be in the order of ethyl acetate>butanol>ethanol>chloroform>aqueous fraction. From the results, we have been able to establish a positive correlation between the antioxidant activity and the total phenolic compound content of the sample. The antioxidant activity in a linoleic acid system was measured using the ferric thiocyanate (FTC) method and thiobarbituric acid (TBA) method. The ethyl acetate fraction had the highest antioxidant activity among the tested fractions. On the basis of these results, the ethyl acetate fraction provided equivalent or higher antioxidant activity as compared to BHT. These results suggest that Sedum sarmentosum is a potentially useful antioxidant for foods, cosmetics, and medicine.

돌나물의 항균활성 및 위암예방효과

최지연 ( Ji Yeon Choi ),김혜민 ( Hye Min Kim ),목소연 ( So Youn Mok ),최경 ( Kyung Choi ),구자정 ( Ja Jung Ku ),박광우 ( Kwang Woo Park ),조은주 ( Eun Ju Cho ),이상현 ( Sang Hyun Lee ) 한국응용생명화학회(구 한국농화학회) 2012 Journal of Applied Biological Chemistry (J. Appl. Vol.55 No.3

The aim of this research was to investigate the industrialapplication of Sedum sarmentosum. Antibacterial activities of then-hexane, methylene chloride (MC), ethyl acetate, and n-butanolfractions of S. sarmentosum were tested against Escherichia coli,Staphylococcus aureus, and Helicobacter pylori. The MC fractionshowed the strongest antibacterial activity against E. coli, with aninhibition zone greater than 13mm in disc assays. At 100ug/mL all fractions scavenged more than 50% of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals (·OH). In particular,the MC fraction showed the strongest scavenging activity against DPPH and ·OH. In addition, we found that treatment with the MC fraction inhibited the growth of H. pylori and gastric adenocarcinomacells. The present results suggest that the MC fraction of S sarmentosum would play the promising protective role againstpathogenic bacteria and free radicals.

돌나물 즙을 첨가한 젤라틴 젤리의 제조 및 품질특성

모은경(Eun Kyoung Mo),김현호(Hyun Ho Kim),김승미(Seung Mi Kim),조현호(Hyun Ho Jo),성창근(Chang Keun Sung) 한국식품과학회 2007 한국식품과학회지 Vol.39 No.6

돌나물(Sedum sarmentosum Bunge)은 영양성분을 많이 함유하고 있는 산채류이나 저장성이 낮고 이를 이용한 가공 식품이 없어 부가가치가 매우 낮은 식품이다. 따라서 본 연구에서는 돌나물의 이용률과 부가가치를 높이고자 돌나물추출액을 첨가하여 젤라틴 젤리를 제조하고, 그 물리화학적 특성을 평가하였다. 돌나물 젤리의 ascorbic acid 함량은 돌나물 즙의 첨가량에 비례하여 그 농도가 증가하였다. 돌나물 젤리에 함유된 양이온 농도를 측정한 결과, 칼슘은 대조구보다 4-28배가 증가하였고, 대조구에 없었던 철분은 각각 0.023-1.031 mg/100 g(젤리 건조 중량)을 함유하는 것으로 분석되었다. 따라서 돌나물 즙 첨가 젤리는 영양소 강화 등 별도의 공정을 거치지 않고도 ascorbic acid, 칼슘, 철분 및 마그네슘 등의 영양소 섭취를 증가시킬 수 있는 것으로 사료되었다. 돌나물 젤리의 녹색도(a value)와 황색도(b value)도 돌나물즙 첨가량이 증가할수록 유의적으로 증가하였으나, 돌나물 즙 2% 첨가구와 3% 첨가구 간에 유의적인 차이는 관측되지 않았다. 돌나물 즙의 첨가량이 증가할수록 젤리(sol 상태)의 pH가 감소하였고, 이에 따라 돌나물 젤리의 hardness와 gumminess가 유의적으로 감소하였으나, 대조구와 0.5-1% 돌나물 즙 첨가구에서는 유의적인 차이가 나타나지 않았으며 이는 관능검사 결과와도 일치하였다. 또한 돌나물 즙을 2% 첨가한 젤리에 대한 선호도가 가장 높은 것으로 나타났다. Sedum sarmentosum, also known as stonecrop (dolnamul), is a widely consumed herb, and is used as an ingredient in salads in Korea. Unfortunately, sedum is perishable and vulnerable to tissue damage during preservation. Therefore, this feasibility study was performed in order to increase the availability of sedum and increase its value. Various concentrations of sedum extract (0.5-3%) were added to gelatin jelly, and their physicochemical properties were determined. The ascorbic acid content of the sedum jelly increased in proportion to the sedum extract concentration. Calcium content of the sedum jelly was 4 to 28 times higher than that of the control. Contrary to the control, iron was detected in the sedum jelly (0.023-1.031 mg/100 g dry weight). Furthermore, magnesium and potassium levels were higher in the sedum extract groups. There were significant differences (p<0.05) in greenness (a value) and yellowness (b value) between the control and the sedum extract groups. However, significant differences between the 2% and 3% sedum extract groups were not detected. As sedum extract concentration increased, the pH level of soft jellies (solid state) decreased. Therefore, hardness and gumminess were decreased significantly. These results are in agreement with the sensory evaluation. According to sensory tests, the values for palatability, appearance, and color in the 2% sedum extract group were higher than those of the 0.5-1% and 3% sedum extract groups.

돌나물추출물에 의한 사람 각질형성세포에서의 Hyaluronan Synthesis 촉진과 인체 피부의 보습력 증진

심관섭 ( Gwan Sub Sim ),김진화 ( Jin Hwa Kim ),이동환 ( Dong Hwan Lee ),나영 ( Young Na ),이근수 ( Geun Soo Lee ),표형배 ( Hyeong Bae Pyo ) 대한화장품학회 2007 대한화장품학회지 Vol.33 No.1

본 연구에서는 돌나물추출물이 각질형성세포에서 hyaluronan synthase (HAS) mRNA 발현과 hyaluronan (HA) 생성에 미치는 영향을 알아보았다. 또한 돌나물추출물이 가지는 인체 피부에서의 보습력 증진 효과를 평가하였다. 돌나물추출물 처리에 따른 각질형성세포에서의 HAS-1, -2, -3 mRNA 발현 변화는 semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR)을 통해 살펴보았고, HA의 생성량 변화는 enzyme-linked immunosorbent assay (ELISA)를 수행하여 확인하였다. 인체 피부에서의 수분함량 및 피부 수분 손실량에 미치는 영향은 Corneometer와 Tewameter를 이용하여 측정하였다. 그 결과, 돌나물추출물은 사람 각질형성세포의 HAS-2, -3 mRNA의 발현을 증가시켰고, HA의 생성량을 농도의존적으로 증가시킴을 확인하였다. 사람 피부에서는 수분 함량을 유지하는 효과가 우수하였으며, 표피 수분 손실량 또한 감소시켜 피부 보습제로서 매우 우수한 효과를 보였다 이상의 결과를 통해 돌나물은 피부에서 HA 생산을 촉진시키며, 피부 보습력을 증진시키는 화장품 소재로 이용될 수 있을 것으로 사료된다. In this study, we investigated the effects of Sedum sarmentosum extract on the expression of hyaluronan synthase (HAS) genes and hyaluronan (HA) production in HaCaT keratinocytes. We also assessed water content (electrical capacitance) and transepidermal water loss (TEWL) in human skin after topical treatment with Sedum sarmentosum extract. Sedum sarmentosum extract increased expression of HAS-2 and HAS-3 genes in HaCaT cells, when assayed by real-time reverse-transcriptase polymerase chain reactions (RT-PCR). Sedum sarmentosum extract induced HA production in HaCaT cells, when determined by enzyme-linked immunosorbent assay (ELISA). Finally, treatment of Sedum sarmentosum extract on human skin increased the skin hydration and decreased TEWL when measured using Corneometer and Tewameter. Our study suggests that Sedum sarmentosum extract should be a very useful cosmetic ingredient, as a skin moisturizer.

돌나물(Sedum sarmentosum) 에탄올 추출물의 항균, 항산화, 항노화 효과와 수중유적형 크림의 제조

김영대 ( Young Dae Kim ),김영민 ( Young Min Kim ),모은경 ( Eun Kyoung Mo ) 대한화장품학회 2017 대한화장품학회지 Vol.43 No.3

돌나물(Sedum sarmentosum)의 기능성과 화장품 소재로서의 특성을 파악하기 위하여 본 연구를 수행하였다. 돌나물을 동결건조하고 분쇄하여 에탄올로 추출한 후 -20 ℃에 저장하면서 실험에 사용하였다. 돌나물의 에탄올 추출물(SE) 내에 포함된 총폴리페놀화합물(TPC)의 함량은 27.98 ± 0.34 g/kg (건조중량)이었고, epicatechin (162.14 ± 5.07 mg/kg), epigallocatechin (55.99 ± 2.49 mg/kg) 및 kaempferol (47.96 ± 3.02 mg/kg)순으로 많이 함유되어 있는 것으로 분석되었다. SE는 organic radical 소거효과(78.43 ± 1.08%)와 금속이온환원능(FRAP value 2.54 ± 0.12)을 나타내었다. 또한 SE는 지질과산화 반응의 초기 단계(FTC assay 62.03 ± 0.38%)와 최종단계(TBARS assay 55.36 ± 2.05%)를 저해하였다. SE (5 mg/mL 건조중량)는 Propionibacterium acne에 대해 항균효과를 나타내었다. SE의 elastase 저해율은 38.94 ± 7.09%이었고, collagenase 저해율은 78.94 ± 2.49%이었다. SE 처리구는 Col3A1 발현양이 증가하였고 collagen 생성율은 대조구보다 58.11 ± 1.07% 높았다. 0.5% SE가 함유된 수중유적형 크림은 pH 6.88, 밀도 1.47 g/mL이었고, 다양한 온도(-20 - 45 ℃)에 4주간 저장하는 동안 크림은 안정한 유화상태를 유지하였다. 따라서 SE는 항균, 항산화, 항노화 활성을 지닌 것으로 사료되었다. This study was performed to investigate the functional properties and characteristics of Dolnamul (Sedum sarmentosum) as a cosmetic ingredient. Lyophilized sedum powder was extracted with ethanol and stored at -20 ℃ for the following experiments. Total polyphenol compounds of the ethanol extract of sedum (SE) was 27.98 ± 0.34 g/kg (dry weight): epicatechin (162.14 ± 5.07 mg/kg), epigallocatechin (55.99 ± 2.49 mg/kg), and kaempferol (47.96 ± 3.02 mg/kg) were contained in the SE. The SE had organic radical scavenging capacity (78.43 ± 1.08%) and metal reducing power (FRAP value 2.54 ± 0.12). FTC and TBARS assays confirmed that the SE inhibited the early stage of lipid peroxidation (62.03 ± 0.38%) as well as the final stage of lipid peroxidation (55.36 ± 2.05%), respectively. The SE (5 mg/mL, dry weight) was proved to have antibacterial effect on the growth of Propionibacterium acnes. The inhibitory percentages of the SE on elastase and collagenase activities were 38.94 ± 7.09% and 78.94 ± 2.49%, respectively. Compare to the control group, the SE treated group induced an increase of Col3A1 expression and collagen production (58.11 ± 1.07%). The oil in water emulsion (0.5% SE adding group) showed pH 6.88 and 1.47 g/mL of density. The hardness changes of the SE adding emulsions were not detected during the stored periods at various temperatures (-20 - 45 ℃) for four weeks. It is considered that the SE has antibacterial, antioxidant, and antiaging activities.

분화 및 옥상 녹화용 내건성 자생화 선발

서종택,유동림,이현숙,이희경,남춘우,류승열,송정섭 한국인간·식물·환경학회 2006 인간식물환경학회지 Vol.9 No.3

도로변 컨테이너 화분용이나 옥상녹화용 내건성 자생식물을 선발하기 위하여 생육이 왕성한 시기에 단수를 한 후 토양수분장력과 관상가능기간을 조사한 결과 시험재료 10종중 무늬둥굴레를 제외한 바위채송화, 돌나물, 연화바위솔, 좀바위솔, 해국, 꿩의비름, 두메부추, 기린초 및 둥근잎꿩의비름 등 9종이 관상가능기간과 생존기간이 길어 적합한 화종으로 나타났다. In ornamental period, Sedum oryzifolium was 60days, Sedum sarmentosum 41days, Orostachys japonicus 51days, Orostachys minutus 53days, Polygonatum odoratum var. pluriflorum 29days, Aster spathulifolius 31day, Sedum erythrostichum 41days, Allium senescens 46days, Sedum kamtschaticum 48days and Sedum rotundifolium 50day. Among wild-flowers of 10 species, 9 species exclude Polygonatum odoratum var. pluriflorum were selected for drought tolerance wild-flowers using of flowerpot and rooftop plant because showed long ornamental period of flower and leaf.

Assessment of Antioxidant Capacity of Sedum (Sedum sarmentosum) as a Valuable Natural Antioxidant Source

모은경,김승미,양선아,Chang Jin Oh,성창근 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.4

In order to investigate the antioxidant capacity of sedum (dolnamul, Sedum sarmentosum) and to offer a scientific basis for the medicinal use of it, lyophilized sedum leaves were extracted with n-hexane, n-butanol,ethyl acetate, and methanol, respectively. The methanol extract (ME) showed the highest extraction yield, but no significant differences in the extraction yield were observed. The total polyphenol content (TPC) of the ethyl acetate extract (EAE) and the ME was significantly higher than that of the non-polar solvent extracts. The EAE and ME showed the superior antioxidant activities in the DPPH, oxygen radical absorbance capacity (ORAC), and ferric reducing ability of plasma (FRAP) assays. Lipid peroxidation was efficiently inhibited by the addition of the sedum extracts in the ferric thiocyanate (FTC) and thiobarbituric acid reactive substances (TBARS) assays,but significant differences among the tested extracts were not observed. The ME showed the potent lipid peroxidation inhibitory activity comparable to butylated hydroxytoluene (BHT). The correlations between the TPC and the antioxidant activities based on the DPPH, ORAC,and FRAP assays were highly positive (R^2>0.899).

Identification of D-Friedoolean-13-en-3-one (Taraxerone) as an Antioxidant Compound from Sedom (Sedum sarmentosum)

모은경,Byung Hee Han,김승미,양선아,강성권,오창진,김란,Cheong Gyu Kim,강효진,성창근 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.2

A pentacyclic triterpenoid compound was isolated from the ethyl acetate extract of sedum (Sedum sarmentosum) and identified as D-friedoolean-13-en-3-one (taraxerone) by GC-MS and crystallographic analysis. The extraction yield of taraxerone was 74.12±0.57 mg/kg sedum (dry weight). The IC50 values of taraxerone were 102.34±1.53 μM and 1,763.81±12.63 μM/mL (Trolox equivalent) by the DPPH and ferric reducing ability of plasma (FRAP) assays, respectively. Taraxerone exhibited comparable antioxidant capacities with butylated hydroxytoluene (BHT) by the DPPH (p=0.117) and FRAP (p=0.179) assays. The production of inducible nitric oxide in lipopolysaccharide-stimulated murine macrophage was inhibited by taraxerone (IC50=38.49±3.77 μM) via downregulation of inducible nitric oxide synthase (iNOS)expression at the transcriptional level. The inhibitory effect of taraxerone on nitric oxide generation was significantly more effective than that of caffeic acid and/or gallic acid.