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Eun-Hee Kim,Jeong-Oh Yang,Doo-Jin Noh,Changmann Yoon,Gil-Hah Kim 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.05
As a major component of cuticle wax, cuticular hydrocarbon plays key roles as a chemical cue among inter- and intra-species in some beetles like as pheromone and alleolochemicals in addition to the physical function. In this study, cuticular hydrocarbon was analyzed and compared with three species of beetles adult, Monochamus alternatus, M. saltuarius belong to the Monochamus sp., and belong to the same class, Moechophyta diphysis, a typical vector of pine wilt disease. The composition of cuticular hydrocarbon of three species of beetles adult did not show the difference between male and female in intra-species, however, showed the difference in inter-species. They also differed from carbon numbers in inter species as 25-32 in M. saltuarius, 25-35 in M. alternatus, and 23-31 in M. diphysis. Major constituent of M. saltuarius was analyzed as n-C25, 2 or 4 MeC26, 9-C25:1, n-C27, 4-MeC28, n-C29, (9,x)-,and/or (11,x)-diMeC29; those of M. alternatus were n-C25, n-C27, 4-MeC28, n-C29, (9,x)-, and/or (11,x)-diMeC29, 9-C29:1, n-C29, (9,x)-,and/or (11,x)-diMeC29; and those of M. diphysis were n-C25, n-C26, n-C27, 3-MeC27,9-C29:1, 11-MeC29. The contents of n-alkanes were as follows: M. saltuarius ≒ M. alternatus > M. diphysis. The contents of monomethylalkanes were as follows: M. diphysis > M. saltuarius ≒ M. alternatus. The contents of dimethylalkanes were as follows: M. saltuarius ≒ M. alternatus > M. diphysis. And the content of olefine in female were analyzed as follows: M. saltuarius > M. alternatus ≒ M. diphysis, and the male's contents were similar in three kinds of beetles.
Tae Young Shin,Sung Min Bae,Jae Bang Choi,Jeong Mi Oh,Jae Kyung Lee,Hyun Na Koo,Soo Dong Woo 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10
Pine wilt is the most important disease of pine trees in Korea, Japan and China. The pathogen causing this disease, the pinewood nematode (Bursaphelenchus xylophylus), is transmitted vectored by adults of some cerambycid beetle species and the Japanese pine sawyer, Monochamus alternatus, is the major vector species in Korea. Although chemical insecticides have been used to kill vector insect and thus prevent transmission of the pathogen, the efficacy is not good. In Japan, to control this insect, an entomopathogenic fungus was studied and developed as an insecticide. This is thought to be the convenient and effective method to control M. alternatus. Recently, there are several reports about the pinewood nematode is vectored by also the pine sawyer, M. saltuarius, in Korea. The objective of this study, therefore, was to isolate and identify entomopathogenic fungi from M. saltuarius cadaver to control it. We collected the cadaver of M. saltuarius and then screened several fungi colonies. The pathogenicity of each fungus was tested using oak longicorn beetle, Moechotypa diphysis, as substitutive insect. M. diphysis is also serious pest to various trees in forest. As the result, only one of them showed high pathogenicity against M. diphysis. Selected fungus was identified by microscopic examination and DNA analysis. Pathogenicity was also evaluated to M. saltuarius.
Do Young Kim,Han-Young Cho,Dong-Ha Shin,Kwang-Hee Son,Ho-Yong Park 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10
A xylan-decomposing Gram-positive bacterium, Cellulosimicrobium cellulans DY-8, was isolated from the gut of a wood-feeding longicorn beetle, Moechotypa diphysis. To amplify a partial fragment of the GH 10 β-1,4- xylanase (XylC) gene of strain DY-8, two degenerated oligonucleotide primers were designed based on strictly conserved regions (WDVVNE and ITELLDV) in the GH family 10 xylanolytic enzymes. The full gene (1488-bp) of XylC, which was predicted to encode a protein consisting of 495 amino acids with a molecular mass of 52.0 kDa and a calculated pI of 6.49, was cloned by repeated DNA walking and nested PCR protocols. The results of a protein blast survey showed that XylC was a β -1,4-xylanase comprised of an N-terminal catalytic GH10 domain (from Ser48 to Leu338) and a C-terminal RICIN domain (from Tyr359 to Leu492). This overall structure of XylC was 57% identical to that of Actinoplanes sp. SE50/110 β -1,4-xylanase (Accession number: YP_006265966), which has not yet been biochemically characterized.
Lim Seong-Keun,Lim Seong-Keun,Hong Soo-Min,Suh Sang Jae,Lee Seung-Yeol,Jung Hee-Young 한국균학회 2023 Mycobiology Vol.51 No.2
The fungal strain belonging to the genus Monochaetia of the family Sporocadaceae was isolated from hairy long-horned toad beetle (Moechotypa diphysis) during the screening of microfungi associated with insects from Gangwon Province, Korea. The strain KNUF-6L2F produced white, light brown to dirty black surface, and olivaceous green colonies with the higher growth, while the closest strain M. ilicis KUMCC 15–0520T were light brown to brown, and M. schimae SAUCC 212201T light brown to brown toward center. The strain KNUF-6L2F produced shorter (5.7–14.0 μm) apical appendages than M. ilicis (6.0–24.0 μm), but similar to M. schimae (7.0–12.5 μm). Three median cells of KNUF-6L2F were light brown to olivaceous green, whereas brown and olivaceous cells were observed from M. ilicis and M. schimae, respectively. And the strain KNUF-6L2F produced larger conidiogenous cells than M. ilicis and M. schimae. Additionally, phylogenetic analyses based on molecular datasets of internal transcribed spacer (ITS) regions, translation elongation factor 1-alpha (TEF1α), and β-tubulin (TUB2) genes corroborated the strain’s originality. Thus, the strain is different from other known Monochaetia species, according to molecular phylogeny and morophology, hence we suggested the new species Monochaetia mediana sp. nov. and provided a descriptive illustration.
Tae Young Shin,Jae Bang Choi,Sung Min Bae,Ye Rim Cha,Hyun Na Koo,Soo Dong Woo 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10
Entomopathogenic fungi were isolated directly from a cadaver of adult M. saltuarius (infected with white fungi) supporting fungal sporulation, to develop biological control of pine wilt disease vector, M. saltuarius which was the most abundant in the middle to northern part of Korea and caused enormous damage to native pine tree in Korea, Japan and other regions of Asia. Pathogenicity of each fungus was tested using oak longicorn beetle, Moechotypa diphysis, as substitutive insect. As the result, only one of them showed pathogenic to adults of M. diphysis, with up to 100% mortality within 13 days of inoculation. Selected fungus was named as MsW1 and identified by Beauveria bassiana using microscopic examination, B. bassiana-specific PCR primers and genetic sequencing of the ITS region analysis. Pathogenicity test were conducted with various concentration of conidial suspensions of this isolate on M. saltuarius (3rd instar larvae and adults). Mortality rates varied from 57.1% to 100.0% and from 16.7% to 100.0% of M. saltuarius (3rd instar larvae and adults), respectively at 30 days. This is the first report of natural infection of M. saltuarius by B. bassiana.
Shin, Tae-Young,Choi, Jae-Bang,Bae, Sung-Min,Cha, Ye-Rim,Oh, Jeong-Mi,Koo, Hyun-Na,Woo, Soo-Dong Korean Society of Sericultural Science 2009 International Journal of Industrial Entomology Vol.18 No.2
Entomopathogenic fungi were isolated directly from a cadaver of adult Monochamus alternatus supporting fungal sporulation, using a semi-selective medium and then screened several fungal colonies. The pathogenicity of each fungus was tested using oak longicorn beetle, Moechotypa diphysis, as substitutive insect. As the result, only one of them showed high pathogenicity against M. diphysis, with up to 100% mortality within 21 days of inoculation. Selected fungus was named as MaW1 and identified by Beauveria bassiana using microscopic examination and DNA analysis. Pathogenicity was also evaluated to M. alternatus.
Tae Young Shin,Jae Bang Choi,Sung Min Bae,Ye Rim Cha,Jeong Mi Oh,Hyun Na Koo,Soo Dong Woo 한국잠사학회 2009 International Journal of Industrial Entomology Vol.18 No.2
Entomopathogenic fungi were isolated directly from a cadaver of adult Monochamus alternatus supporting fungal sporulation, using a semi-selective medium and then screened several fungal colonies. The pathogenicity of each fungus was tested using oak longicorn beetle, Moechotypa diphysis, as substitutive insect. As the result, only one of them showed high pathogenicity against M. diphysis, with up to 100% mortality within 21 days of inoculation. Selected fungus was named as MaW1 and identified by Beauveria bassiana using microscopic examination and DNA analysis. Pathogenicity was also evaluated to M. alternatus.