大敦?少衝?少府 刺鍼이 腦血流力學에 미치는 영향

박은주,조명래 대한침구의학회 2007 대한침구의학회지 Vol.24 No.1

Objection : The aim of this study was to investigate anti-ischemic effect of LR1,HT8,HT9 Reinforcement in Acupuncture. Methods : I designed to investigate whether LR1,HT8,HT9 Reinforcement in Acupuncture affects cerebral hemodynamics〔regional cerebral blood flow(rCBF), pial arterial diameter(PAD), mean arterial blood pressure(MABP)〕in normal and cerebral ischemia rats by MCA occlusion method, and to make manifest whether LR1,HT8,HT9 Reinforcement in Acupuncture is mediated by cyclooxygenase or guanylate cyclase. The changes of rCBF and MABP were determinated by laser-doppler flowmetry(LDF), and the change of PAD was determinated by video microscope and width analyzer. Results : The results were as follows ; 1. LR1,HT8,HT9 Reinforcement in Acupuncture was significantly increased rCBF, PAD, but decreased MABP after withdrawing of the needle. This results suggest that LR1,HT8,HT9 Reinforcement in Acupuncture increased significantly rCBF by dilating PAD. 2. Pretreatment with indomethacin(1㎎/㎏, i.v.) was significantly inhibited LR1,HT8,HT9 Reinforcement in Acupuncture induced increase of rCBF and PAD, but increased LR1,HT8,HT9 Reinforcement in Acupuncture induced decrease of MABP after withdrawing of the needle. 3. Pretreatment with methylene blue(10㎍/㎏, i.v.) was decreased LR1,HT8,HT9 Reinforcement in Acupuncture induced increase of rCBF and MABP, but accelerated LR1,HT8,HT9 Reinforcement in Acupuncture induced decrease of PAD. This results suggest that the mechanism of LR1,HT8,HT9 Reinforcement in Acupuncture is mediated by cyclooxygenase or guanylate cyclase. Conclusion : I suggest that LR1,HT8,HT9 Reinforcement in Acupuncture has an anti-ischemic effect through the improvement of cerebral hemodynamics, and the mechanism is mediated by cyclooxygenase.

대돈,소부 사법자침이 뇌혈류력학 변동 개선에 미치는 영향

신동훈,조명래 대한침구의학회 2004 대한침구의학회지 Vol.21 No.1

Experimental Study of Dadun(LR1)·Shaofu(HT8) Reduction in Acupuncture on the Improvement of Cerebral Hemodynamics Objective : The aim of this study was to investigate anti-ischemic effect of LR1·HT8 Reduction in Acupuncture Methods : I designed to investigate whether LR1·HT8 Reduction in Acupuncture affects cerebral hemodynamics〔regional cerebral blood flow(rCBF), pial arterial diameter(PAD), mean arterial blood pressure(MABP)〕in normal rats and to make manifest whether LR1·HT8 Reduction in Acupuncture is mediated by cyclooxygenase or guanylate cyclase. The changes of rCBF and MABP were determinated by laser-doppler flowmetry(LDF), and the change of PAD was determinated by video microscope and width analyzer. Results : The results were as follows ; 1. LR1 Reduction in Acupuncture was increased rCBF and PAD, but decreased MABP. 2. HT8 Reduction in Acupuncture was significantly increased rCBF, but decreased MABP, and increased PAD. 3. LR1·HT8 Reduction in Acupuncture was significantly increased rCBF, PAD, but decreased MABP after withdrawing of the needle. This results suggest that LR1·HT8 Reduction in Acupuncture increased significantly rCBF by dilating PAD. 4. Pretreatment with indomethacin(1㎎/㎏, i.v.) was significantly inhibited LR1·HT8 Reduction in Acupuncture induced increase of rCBF and PAD, but increased LR1·HT8 Reduction in Acupuncture induced decrease of MABP after withdrawing of the needle. 5. Pretreatment with methylene blue(10㎍/㎏, i.v.) was decreased LR1·HT8 Reduction in Acupuncture induced increase of rCBF and PAD, but accelerated LR1·HT8 Reduction in Acupuncture induced decrease of MABP. Conclusions : I suggest that LR1·HT8 Reduction in Acupuncture has an anti-ischemic effect through the improvement of cerebral hemodynamics, and the mechanism is mediated by cyclooxygenase.

행간(行間)·소부(少府) 보사침법(補瀉鍼法)이 국소뇌혈류량(局所腦血流量) 및 평균혈압(平均血壓)에 미치는 영향(影響)

신정철,유충렬,조명래,Shin, Jeong-cheol,Ryu, Chung-ryul,Cho, Myeng-rae 대한침구의학회 2003 대한침구의학회지 Vol.20 No.6

Objective: The purpose of this study is to examine influences by the order, Reduction and Reinforcement in Acupuncture on cerebral hemodynamics [regional cerebral blood flow(rCBF) and mean arterial blood pressure(MABP)] in normal rats. Methods: This experiments was to to investigate eath other changes of rCBF and MABP at Xingjian(LR2)(1st) Shaofu(HT8)(2nd) Reduction, Xingjian(LR2)(1st) Shaofu(HT8)(2nd) Reinforcement, Shaofu(HT8)(1st) Xingjian(LR2)(2st) Reduction and Shaofu(HT8)(1st) Xingjian(LR2)(2st) Reduction in Acupuncture. Results: 1. LR2(1st) HT8(2nd) Reduction in Acupuncture was decreased rCBF and MABP in compared with normal condition. 2. LR2(1st) HT8(2nd) Reinforcement in Acupuncture was significantly decreased rCBF, and was decreased MABP in compared with normal condition. 3. HT8(1st) LR2(2st) Reduction in Acupuncture was decreased rCBF during acupuncture but was recovered rCBF after with-drawing of the neddle. 4. HT8(1st) LR2(2st) Reduction in Acupuncture was decreased MABP during acupuncture and after withdrawing of the neddle. 5. HT8(1st) LR2(2st) Reinforcement in Acupuncture was significantly increased rCBF during acupuncture and 30min after withdrawing of the neddle. 6. HT8(1st) LR2(2st) Reinforcement in Acupuncture was decreased MABP during acupuncture, but was recovered MABP after withdrawing of the neddle. Conclusions: I suggested that LR2 HT8 Reduction in Acupuncture and LR2 HT8 Reinforcement in Acupuncture cause a diverse response of cerebral hemodynas.

대돈(大敦)·소부(少府) 사법자침(瀉法刺鍼)이 뇌혈류역학(腦血流力學) 변동(變動) 개선(改善)에 미치는 영향

신동훈,조명래,Shin, Dong-hoon,Cho, Myoung-rae 대한침구의학회 2004 대한침구의학회지 Vol.21 No.1

Objective: The aim of this study was to investigate anti-ischemic effect of LR1 HT8 Reduction in Acupuncture Methods: I designed to investigate whether LR1 HT8 Reduction in Acupuncture affects cerebral hemodynamics [regional cerebral blood flow(rCBF), pial arterial diameter(PAD), mean arterial blood pressure(MABP)] in normal rats and to make manifest whether LR1 HT8 Reduction in Acupuncture is mediated by cyclooxygenase or guanylate cyclase. The changes of rCBF and MABP were determinated by laser-doppler flowmetry(LDF), and the change of PAD was de terminated by video microscope and width analyzer. Results: The results were as follows; 1. LR1 Reduction in Acupuncture was increased rCBF and PAD, but decreased MABP. 2. HT8 Reduction in Acupuncture was significantly increased rCBF, but decreased MABP, and increased PAD. 3. LR1 HT8 Reduction in Acupuncture was significantly increased rCBF, PAD, but decreased MABP after withdrawing of the needle. This results suggest that LR1 HT8 Reduction in Acupuncture increased significantly rCBF by dilating PAD. 4. Pretreatment with indomethacin(1mg/kg, i,v.) was significantly inhibited LR1 HT8 Reduction in Acupuncture induced increase of rCBF and PAD, but increased LR1 HT8 Reduction in Acupuncture induced decrease of MABP after withdrawing of the needle. 5. Pretreatment with methylene blue($10{\mu}g/kg$, i,v.) was decreased LR1 HT8 Reduction in Acupuncture induced increase of rCBF and PAD, but accelerated LR1 HT8 Reduction in Acupuncture induced decrease of MABP. Conclusions: I suggest that LR1 HT8 Reduction in Acupuncture has an anti-ischemic effect through the improvement of cerebral hemodynamics, and the mechanism is mediated by cyclooxygenase.

《난경(難經)》 오사론(五邪論)을 적용한 침법(鍼法)이 2K1C 고혈압(高血壓) 백서(白鼠)의 혈압(血壓)에 미치는 영향(影響)

박은주,나창수,윤여충,조명래,Park, Eun-ju,Na, Chang-su,Yun, Yeu-chung,Cho, Myung-rae 대한침구의학회 2003 대한침구의학회지 Vol.20 No.1

Objectives : The purpose of this study is to compare Xingjian(LR2) Shaofu(HT8) with Dadun(LR1) Shaofu(HT8) on Blood Pressure in Hypertensive RAT induced by Two Kidney One Clip(2KIC). Methods : This experiments was to investigate the effects of LR2 HT8(originated from ${\ll}$Classic on Difficulty${\gg}$ Shi Ze Xie Qi Zi), LR1 HT8(originated from ${\ll}$Ling Shu${\gg}$ Sheng Ze Xie Zhi) acupuncture on the blood pressure, cardiomegalic index, and plasma levels of atrial natriuretic peptide in hypertensive rat induced by 2K1C. Results: 1. Blood pressure was decreased significantly after third acupuncture of LR2 HT8. 2. Blood pressure was decreased significantly after acupuncture of LR2 HT8, but was increased after LR2 HT8. 3. Cardiomegalic index was not changed after acupuncture of LR2-HT8 and LR1-HT8 4. Plasma levels of atrial natriuretic peptide was increased significantly after acupuncture of LR2 HT8 but LR1 HT8 was not changed.

족소음신경(足少陰腎經) 연곡(然谷),음곡(陰谷)과 수소음심경(手少陰心經) 소부(少府),소해(少海) 침자(鍼刺)가 신장(腎臟) Aquaporin-2 발현(發現)과 신교감신경활성(腎交感神經活性), 혈압(血壓)및 혈청(血淸) ANP, renin, norepinephrine에 미치는 영향(影響)

윤대환 ( Dae Hwan Youn ),박찬규 ( Chna Kyu Park ),김정상 ( Jeong Sang Kim ),채우석 ( Woo Seok Chae ),나창수 ( Chang Su Na ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.21 No.1

Objectives: This research was performed to investigate the effect of acupuncture at the KI2, KI10, HT8, HT3 on Aquaporin-2(AQP2) expression related with the renal functions in rats. Methods: Acupuncture was performed during 100-seconds, 6-times, at 150-seconds intervals under anesthesia in rats. We observed rats` mean arterial pressure(MAP), heart rate(HR), renal sympathetic nerve activity(RSNA) during acupuncture and AQP2 expression by western blot method and atrial natriuretic peptide(ANP), renin, norepinephrine of plasma after decapitation. Results: The AQP2 expression was significantly increased in HT8 group, but decreased in KI10 group. Average MAP during 6-times acupuncture was significantly increased in HT8 group. Average HR was significantly increased in HT8 group, Average RSNA was increased in KI10 group, but that was marginally increased in KI10 group. Plasma renin concentration was increased in KI2 , HT3 groups. Plasma ANP show a tendency to decrease in KI10, HT3 groups, increased in KI2 , HT8 but not significant. Plasma norepinephrine concentration was significantly decreased in KI10, HT3 groups. Conclusions: These results suggested that acupuncture at HT8 activate renal function to reuptake, but KI10 show a decline on effect of AQP2 expression, blood pressure, nerve activity and renin.

소부(HT8) 전침이 뇌파(EEG)와 심박변이도(HRV)에 미치는 영향

윤대식 ( Dae Shik Yoon ),홍승원 ( Seung Won Hong ),이용섭 ( Yong Sub Lee ) 경락경혈학회 2013 Korean Journal of Acupuncture Vol.30 No.4

Objectives: The aim of this study was to examine the effect of electroacupuncture (EA) at an acupoint, HT8 (Sobu), on normal humans by using power spectral analysis. We examined the effect on the Heart Rate Variability (HRV), and the balance of the autonomic nervous system. Methods: Thirty-two healthy volunteers participated in this study. EEG (Electroencephalogram) power spectrum exhibits site-specific and state-related differences in specific frequency bands. A thirty-two channel EEG study was carried out on thirty-two subjects (14 males; mean age=23.5 years old, 18 females; mean age=21.5 years old). HRV and EEG were simultaneously recorded before and after acupuncture. Results: In the α(alpha) band, during the HT8-acupoint treatment, the power values in the α(alpha) band significantly decreased(p<0.05) at 28 channels. In the β(beta) band significantly decreased(p< 0.05) at 26 channels. In δ(delta) band significantly decreased(p<0.05) at 18 channels. In θ(theta) band significantly decreased(p< 0.05) at 20 channels. α/β values were increased at 6 channels and decreased at 10 channels. β/θ values were increased at 10 channels and decreased at 19 channels. Mean-RR(RR-interval), Complexity, RMSSD(Root mean square of successive differences), SDSD(Standard deviations differences between adjacent normal R-R intervals), norm HF showed a significantly increased and mean-HRV, norm LF, LHR(LF/HF Ratio) showed a significantly decreased after HT8-acupoint treatment(p<0.05). Conclusions: These results suggest that EA at the HT8 mostly causes significant changes on alpha (28 channels), beta (26 channels), delta(18 channels), theta(20 channels) bands and mean-HRV, mean-RR, complexity, RMSSD, SDSD, norm LF, norm HF and LHR. If practicing EA at the HT8, it will regulate the function of the cerebral cortex, decrease activity of the sympathetic and increase parasympathetic nervous activity.

데옥시콜릭산에 의한 HT-29 대장상피세포의 신호전달 -NF-κB와 Interleukin-8의 역할-

이동기 ( Lee Dong Gi ),박선영 ( Park Seon Yeong ),백순구 ( Baeg Sun Gu ),권상옥 ( Kwon Sang Og ),정준모 ( Jeong Jun Mo ),오억수 ( O Eog Su ),김현수 ( Kim Hyeon Su ) 대한소화기학회 2004 대한소화기학회지 Vol.43 No.3

Background/Aims: Deoxycholic acid (DCA) has been appeared to be an endogenous colon tumor promoter. In this study, we investigated whether DCA induces nuclear factor-kappa B (NF-kB) activation and IL-8 expression, and tauroursodeoxycholic acid (TUDC) inhibits this signaling in HT-29 cells. Methods: After DCA treatments, time courses of NF-kB binding activity were determined by electrophoretic mobility shift assay (EMSA). Also, we performed Western blotting of IkBα to confirm NF-kB activation. Time and concentration courses of DCA-induced secretion of IL-8 were measured with ELISA in supernatants of cultured media from the cells. To evaluate the role of NF-kB, IL-8 levels were assessed after pretreatment with using phosphorothioate-modified anti-sense oligonucleotides (ODN). Moreover, DCA-induced secretions of IL-8 were measured after pretreatment with TUDC. Results: DCA dose-dependently induced prominent NF-kB binding complexes from 30 min to 8 hr and degradation of IkBα. The secretions of IL-8 were increased with DCA (50~200 μM) treatment in a time and dose-dependent manner. Pre-incubation of the cells with TUDC (0.1~10 μM) for 2 hours caused significant decreases in DCA induced IL-8 secretion. However, transient transfection using p50 or p65 AS-ODN showed no effect on IL-8 secretion. Conclusions: DCA may play as a colonic tumor promoter through anti-apoptotic effect of NF-kB activation and IL-8 expression, and DCA-induced NF-kB independent IL-8 expression is inhibited by TUDC. (Korean J Gastroenterol 2004;43:176-185)

곤륜(崑崙),소부(少府) 침자(鍼刺)가 하장간막신경(下腸間膜神經) 활성(活性)과 혈압(血壓) 및 심박수(心搏數)에 미치는 영향(影響)

나창수 ( Chang Su Na ),리강욱 ( Kang Uk Lee ),윤여충 ( Yeo Chung Yun ),김정상 ( Jeong Sang Kim ) 대한경락경혈학회 2002 Korean Journal of Acupuncture Vol.19 No.2

Objectives: The aim of the present study is to observe effects of acupuncture related with the nerve system, Inferior mesenteric nerve activity(IMNA) was examined on BL60 acupuncture closely connected with the bladder and HT8 with little tied. Materials and methods: Acupunctures were performed during 100 seconds after stabilization. IMNA was measured by micromanipulator, preamplifier, mean arterial pressure(MAP) and heart rate were observed by blood pressure transducer, 4-channel transducer amplifier in anesthetized rat. Results: On BL60 acupuncture, the heart rate was not significantly decreased but IMNA, MAP were significantly decreased and Maximum peak of IMNA was increased during inserted acupuncture, decreased after ejected acupuncture respectively. On HT8 acupuncture, IMNA was decreased during acupuncture than before acupuncture but the others did not showed signigicant difference. Conclusions: Our results demonstrated a meridian interaction between BL60 acupoint in the bladder channel and Inferior mesenteric sympathetic nerve related to the bladder. This fact suggest that BL60 acupuncture have effect on the bladder through the nerve system. Meanwhile, IMNA was decreased during HT8 acupuncture. This result is supposed HT8 to have not meridian function but acupoint function by another mechanism. It needs to be closely examined other meridians and nerve by the optimal approaching method.

염전(捻轉) 및 영수(迎隨) 사법(瀉法)에 의해서 시행된 소부(少府),행간(行間) 침자(鍼刺)가 고지방식이(高脂肪食餌)로 유발된 비만(肥滿) 백서(白鼠)에 미치는 영향(影響)

최태진 ( Tae Jin Choi ),황문현 ( Moon Hyeon Hwang ),임선주 ( Seon Joo Ihm ),김성옥 ( Sung Ok Kim ),나창수 ( Chang Su Na ),최진봉 ( Jin Bong Choi ),윤대환 ( Dae Hwan Youn ) 한방재활의학과학회 2008 한방재활의학과학회지 Vol.18 No.4

Objectives : In this research, it was investigated that had effects on acupuncture techniques of HT8(Shaofu) and LR2(Xingjian) acupoints in high fat rats. We recorded data of weight gain, food intake, food efficiency, total cholesterol, triglyceride, AST, ALT, r-GTP, total bilirubin on rats fed high fat diet for 10 weeks. Methods : We divided experimental groups into four. Group of high-fat diet feeding and no treatment was control, group of high-fat diet feeding and acupuncture at HT8·LR2 was Acu-1, group of high-fat diet feeding and acupuncture at HT8·LR2 and rotated counter-clockwise 36times was Acu-2, high-fat diet feeding and acupuncture at HT8(body direction)·LR2(lower limb direction) and rotated counter-clockwise 36times was Acu-3. Results : In Acu-3 group, body weight on 2 weeks after starting acupuncture, food efficiency and the level of serum total cholesterol were decreased, and the level of HDL-cholesterol was increased. In Acu-1 group, the level of triglyceride was increased but ALT was decreased. Conclusions : These results suggest that treatment of acupuncture at HT8(body direction)·LR2(lower limb direction) and rotated counter-clockwise 36times may be having the influence on high fat by controlling the level of serum total cholesterol and HDL-cholesterol.