국내산 울금, 강황 및 보라울금의 항균활성과 항산화 효과

김현진 ( Hyun Jin Kim ),이중원 ( Jung Won Lee ),김용두 ( Yong Doo Kim ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2011 한국식품저장유통학회지 Vol.18 No.2

We investigated the antimicrobial and antioxidant activities, and total polyphenol contents of Curcuma longa, Curcuma aromatica, and Curcuma zedoaria. In antimicrobial activity assays of solvent extracts, ethanol extract showed the highest activity compared with other extracts. The antimicrobial activity of ethanol extract of C. longa showed higher than those of C. aromatica, and C. zedoaria. Antimicrobial substance in ethanol extract of C. longa was maintained after heating at 100℃ for 30 min and not affected by changes in pH. DPPH free radical scavenging activity of solvent extracts were high in the order of ethanol, ethylacetate, ether, water, and hexane fractions. C. longa showed greater DPPH free radical scavenging activity than those of the C. aromatica, and C. zedoaria. Total polyphenol content of C. longa and C. aromatica exhibited were similar levels, whereas C. zedoaria was slightly lower concentration.

Curcuzedoalide contributes to the cytotoxicity of <i>Curcuma zedoaria</i> rhizomes against human gastric cancer AGS cells through induction of apoptosis

Jung, Eun Bee,Trinh, Tuy An,Lee, Tae Kyoung,Yamabe, Noriko,Kang, Ki Sung,Song, Ji Hoon,Choi, Sungyoul,Lee, Sanghyun,Jang, Tae Su,Kim, Ki Hyun,Hwang, Gwi Seo Elsevier 2018 Journal of Ethnopharmacology Vol.213 No.-

<P><B>Abstract</B></P> <P><B>Ethnopharmacological relevance</B></P> <P> <I>Curcuma zedoaria</I> Roscoe (Zingiberaceae), also known as white turmeric or zedoaria, has been used in Ayurveda and traditional Chinese medicine to treat various cancers, and it possesses several sesquiterpenoid compounds.</P> <P><B>Objective</B></P> <P>This study aimed to evaluate the therapeutic effects of a methanolic (MeOH) extract of <I>C. zedoaria</I> rhizomes, as well as its active constituents, against gastric cancer, which is a frequently diagnosed cancer in South Korea.</P> <P><B>Materials and methods</B></P> <P>Repeated column chromatography, together with semi-preparative HPLC purification, was used to separate the bioactive constituents from the <I>C. zedoaria</I> MeOH extract. The cytotoxic effects of the <I>C. zedoaria</I> MeOH extract and its active compounds were measured in human gastric cancer AGS cells. Expression of proteins related to apoptosis was evaluated using Western blotting analysis.</P> <P><B>Results</B></P> <P>The MeOH extract of <I>C. zedoaria</I> rhizomes exerted a cytotoxic effect on AGS cells (IC<SUB>50</SUB>: 96.60 ± 4.87μg/mL). Based on the bioactivity-guided fractionation for antiproliferative activity, a chemical investigation of the MeOH extract led to the isolation of five sesquiterpenes including isoprocurcumenol (<B>1</B>), germacrone (<B>2</B>), curzerenone (<B>3</B>), curcumenol (<B>4</B>), and curcuzedoalide (<B>5</B>). Among these, curcuzedoalide demonstrated the strongest effect in suppressing gastric cancer cell proliferation in a dose-dependent manner with an IC<SUB>50</SUB> value of 125.11±2.77μM. Western blotting analysis showed that curcuzedoalide inhibited AGS human gastric cancer cell viability by activating caspase-8, caspase-9, caspase-3, and PARP, which contributed to apoptotic cell death in AGS human gastric cancer cells.</P> <P><B>Conclusion</B></P> <P>These data indicate that curcuzedoalide contributed to the cytotoxicity of <I>C. zedoaria</I> by activating the cleavage of caspases and PARP, which are representative markers for apoptosis. Therefore, curcuzedoalide is a positive candidate for the development of novel chemotherapeutics.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Bioactivity-based analysis and chemical characterization of anti-inflammatory compounds from <i>Curcuma zedoaria</i> rhizomes using LPS-stimulated RAW264.7 cells

Lee, Tae Kyoung,Trinh, Tuy An,Lee, Seoung Rak,Kim, Sil,So, Hae Min,Moon, Eunjung,Hwang, Gwi Seo,Kang, Ki Sung,Kim, Ji Hwan,Yamabe, Noriko,Kim, Ki Hyun Elsevier 2019 Bioorganic chemistry Vol.82 No.-

<P><B>Abstract</B></P> <P>Inflammation is not only a self-defense response of the innate immune system, but also the pathogenesis mechanism of multiple diseases such as arthritis, neurodegeneration, and cancer. <I>Curcuma zedoaria</I> Roscoe (Zingiberaceae), an indigenous plant of India, has been used traditionally in Ayurveda and folk medicine. As part of our ongoing efforts to screen traditional medicinal plants exhibiting pharmacological potential and to characterize the compounds involved, we examined the anti-inflammatory effects of the MeOH extract of <I>C. zedoaria</I> rhizomes using lipopolysaccharide (LPS)-stimulated RAW264.7 murine macrophage cells and found that MeOH extract inhibited the synthesis of nitric oxide (NO) in a dose-dependent manner (IC<SUB>50</SUB>: 23.44 ± 0.77 μg/mL). In our efforts to characterize the compounds responsible for these anti-inflammatory effects, bioactivity-guided fractionation of the MeOH extract and chemical investigation of its active hexane-soluble fraction led to the successful isolation of five sesquiterpenes (<B>1</B>–<B>5</B>), the structures of which were elucidated by NMR spectroscopic analysis and LC/MS analysis. Among them, curcuzedoalide (<B>5</B>) exhibited potent inhibitory effects on NO synthesis (IC<SUB>50</SUB>: 12.21 ± 1.67 μM) and also suppressed pre-inflammatory protein expression of iNOS and COX-2. Curcuzedoalide (<B>5</B>) was thus determined to be a contributor to the anti-inflammatory effect of <I>C. zedoaria</I> rhizomes and could be a potential candidate for therapeutic applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> MeOH extract of <I>Curcuma zedoaria</I> rhizomes inhibited the synthesis of nitric oxide (NO). </LI> <LI> Five sesquiterpenes (<B>1</B>–<B>5</B>) were isolated using bioactivity-based analysis. </LI> <LI> Curcuzedoalide (<B>5</B>) exhibited potent inhibitory effects on NO synthesis. </LI> <LI> The compound (<B>5</B>) suppressed pre-inflammatory protein expression of iNOS and COX-2. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Curcuma zedoaria (christm.) roscoe inhibits proliferation of MDA‑MB231 cells via caspase‑cascade apoptosis

Romen Meitei Lourembam,Amit Singh Yadav,Gopal C. Kundu,Pranab Behari Mazumder 경희대학교 융합한의과학연구소 2019 Oriental Pharmacy and Experimental Medicine Vol.19 No.3

Curcuma zedoaria is a perennial herb that belongs to Zingiberaceae family, found growing lavishly in Manipur; a north eastern part of India. The traditional usages of the plant is not limited to a particular disease but extended over a large number of diseases. Anti-breast cancer activity of ethyl acetate extract of Curcuma zedoaria was conducted on MDA-MB231 breast cancer cell line with the help of MTT assay technique and the mechanistic pathway was established with the help of western blot technique, confocal microscopy, wound healing migration assay and cell cycle analysis. Cell cytotoxicity test of ethyl acetate extracts showed remarkable result on MDA-MB231 in dose dependent manner (p<0.05). Confocal microscopy studies on MDA-MB231 cells revealed the activation of cleaved caspase 9 in treated cells. Western blot analyses confrm the regulation of cytochrome C and suppression of Bcl-2 in treated cells which suggest ethyl acetate extract of Curcuma zedoaria inhibits the cancer cells through cascase-dependent pathways. In cell cycle analysis, after the treatments of the cells; there was an increase in the percentage of SubG0/G1 cells that decreased S and G2 phase indicating the population undergoing cell death (apoptosis or necrosis) thereby, our data suggest that ethyl acetate extracts were able to induce signifcant apoptosis in MDA-MB-231 cells in dose dependent manner. The wound healing migration assay confrms the anti-metastatic nature of the plant extract. With the help one way ANOVA test, all the experiments were executed and it was found that ethyl acetate extract of Curcuma zedoaria inhibits MDA-MB231 cells with the down-regulation of the expression of Bcl-2 through cascase-dependent pathways therefore Curcuma zedoaria is a potent anti-cancer plant especially in breast cancer.

Extract of Curcuma zedoaria R. prevents atherosclerosis in apolipoprotein E-deficient mice

Ki Mo Kim,Joo Young Lee,Byeong Hwa Jeon,Khong Trong Quan,MinKyun Na,Kung-Woo Nam,Sungwook Chae 한국영양학회 2021 Nutrition Research and Practice Vol.15 No.3

BACKGROUND/OBJECTIVES: Curcuma zedoaria R. (Zingiberaceae) has been used to treat headache, fever, and hypertension-related symptoms in Asian countries, including Korea, China, and Japan. We investigated whether dietary intake of a C. zedoaria extract (CzE) affected atherosclerosis in vivo. MATERIALS/METHODS: Apolipoprotein E-deficient (ApoE<SUP>−/−</SUP>) mice (n = 32) were fed a normal diet (ND), a high-cholesterol diet (HCD), an HCD containing CzE (100 mg/kg/day), or an HCD containing simvastatin (10 mg/kg/day) for 12 weeks. The anti-atherosclerotic effects were evaluated by observing changes in fatty streak lesions, immunohistochemical analysis, ex vivo fluorescence imaging, lipid profiles, and western blot analysis. RESULTS: The CzE-fed group showed a 41.6% reduction of atherosclerosis. Furthermore, CzE significantly reduced the levels of serum triglyceride, high-density lipoprotein, the chemokine (C-X3-C-motif ) ligand 1, the adhesion molecules vascular cell adhesion molecule-1, intracellular adhesion molecule-1, and E-selectin; down-regulation of tumor necrosis factor-α, interleukin-6, high mobility group box-1, and cathepsin levels in the aortic sinuses and aortas of ApoE<SUP>−/−</SUP> mice were also observed. CONCLUSIONS: The results suggest that the inclusion of a water extract of C. zedoaria in a HCD is closely correlated with reducing the risk of vascular inflammatory diseases in an ApoE mouse model.

Cytotoxic Activity of the Extracts from Curcuma zedoaria

Kim, Myoun-Gae,Kim, Jung-Sun,Hong, Jon-Ki,Ji, Ming-Jie,Lee, Yong-Kyu Korean Society of ToxicologyKorea Environmental Mu 2003 Toxicological Research Vol.19 No.4

The effect of the hexane extract of Curcuma zedoaria roots and its solvent fractions were investigated on the proliferation of SiHa, SNU-1 and HepG2 cell lines. Among those fractions, final fraction H2-3-1 and H2-3-3 showed cytotoxic effect on SiHa and HepG2 cell lines. The hallmark of apoptosis, DNA fragmentation, also appeared in the final fractions H2-3-1 and H2-3-3 after 24h treatment in SiHa cell line. Furthermore, those fractions were shown to be able to induce cell death in $[^3H]$thymidine incorporation test. These two fractions, H2-3-1 and H2-3-3 were determined as (-)-$\alpha$-curcumene and $\beta$-tumerone respectively by NMR and mass spectrum. From these results, it is speculated that te hexane extract of Curcuma zedoaria is necessary for further studies as a potent inhibitor of the growth of cancer cells.

A Curcuminoid and Two Sesquiterpenoids from Curcuma zedoaria as Inhibitors of Nitric Oxide Synthesis in Activated Macrophages

Jang, Mi-Kyung,Lee, Hwa-Jin,Kim, Ji-Sun,Ryu , Jae-Ha The Pharmaceutical Society of Korea 2004 Archives of Pharmacal Research Vol.27 No.12

The overproduction of nitric oxide (NO) by inducible nitric oxide synthase (iNOS) is known to be responsible for vasodilation and hypotension observed in septic shock and inflammation. Inhibitors of iNOS, thus, may be useful candidates for the treatment of inflammatory diseases accompanied by overproduction of NO. In the course of screening oriental anti-inflammatory herbs for the inhibitory activity of NO synthesis, a crude methanolic extract of Curcuma zedoaria exhibited significant activity. The activity-guided fractionation and repetitive chromatographic procedures with the EtOAc soluble fraction allowed us to isolate three active compounds. They were identified as 1,7-bis (4-hydroxyphenyl)-1,4,6-heptatrien-3-one (1), procurcumenol (2) and epiprocurcumenol (3) by spectral data analyses. Their concentrations for the 50% inhibition of NO production $(IC_{50})$ in lipopolysaccharide (LPS)-activated macrophages were 8, 75, 77 ${\mu}M$, respectively. Compound 1 showed the most potent inhibitory activity for NO production in LPS-activated macrophages, while the epimeric isomers, compound 2 and 3 showed weak and similar potency. Inhibition of NO synthesis by compound 1 was very weak when activated macrophages were treated with 1 after iNOS induction. In the immunoblot analysis, compound 1 suppressed the expression of iNOS in a dose-dependent manner. In summary, 1,7-bis (4-hydroxyphenyl)-1,4,6-heptatrien-3-one from Curcuma zedoaria inhibited NO production in LPS-activated macrophages through suppression of iNOS expression. These results imply that the traditional use of C. zedoaria rhizome as anti-inflammatory drug may be explained at least in part, by inhibition of NO production.

Sesquiterpenoids from the Rhizome of Curcuma zedoaria

Hong, Chai Hee,Kim, Youngleem,Lee, Sang Kook 梨花女子大學校 藥學硏究所 2001 藥學硏究論文集 Vol.- No.10

In the course of searching for biologically active sesquiterpenoids from Curcuma genus, twoo sesquiterpenoids were isolated from the rhizome of Curcuma zedoaria(Zingiberaceae). Their structures were identified as ar-turmerone(1) and β-turmerone(2). The structure elucidation of compounds 1 and 2 was carried out by comparison of their physical and spectral data with previously reported values.

Antibiofilm Activity of a Curcuma zedoaria Rosc Rhizome Extract against Methicillin-Resistant and Susceptible Staphylococcus aureus

Tabunhan, Sompong,Tungsukruthai, Parunkul The Korean Society for Microbiology and Biotechnol 2022 한국미생물·생명공학회지 Vol.50 No.2

Methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) are major causes of hospital- and community-acquired infections. The treatment of biofilm-related infections caused by these bacteria is a global healthcare challenge. Therefore, the development of alternative therapeutics is required. An essential oil extracted from Curcuma zedoaria (CZ) Rosc, also known as white turmeric, has been reported to possess various antimicrobial activities. In the present study, we evaluated the antibiofilm activities of an ethanolic extract of the CZ rhizome against MRSA and MSSA. The results showed that the CZ extract with the highest sub-minimum inhibitory concentration (sub-MIC), 1/2 MIC (0.312 mg/ml), significantly inhibited biofilm production by up to 80-90% in both tested strains. Subsequently, we evaluated the ability of the CZ extract to prevent cell-surface attachment to a 96-well plate and extracellular DNA (eDNA) release from the biofilm. The CZ extract demonstrated an inhibitory effect on bacterial attachment and eDNA release from the biofilm biomass. The CZ extract may inhibit biofilm formation by preventing eDNA release and cell-surface attachment. Therefore, this CZ extract is a potential candidate for the development of alternative treatments for biofilm-associated MRSA and MSSA infections.

아출(莪朮)의 Sesquiterpene 성분의 약물활성

신국현,윤기영,조태순,Shin, Kuk-Hyun,Yoon, Ki-Young,Cho, Tae-Soon 한국생약학회 1994 생약학회지 Vol.25 No.3

Pharmacological activities of two sesquiterpenes, curzerenone(I), and curcumenol(II) isolated from the rhizomes of Curcuma zedoaria being used as an aromatic stomachic in Chinese medicines were evaluated in rats and mice. Curzerenone (I), at 100mg/kg, p.o. showed a potent protective effect against HCl-ethanol induced gastric lesion in rats, and curcumenol(II), at the same dose level showed a CNS depressant action characterized by a potentiation of hexobarbital(HB)-induced narcosis. Both compounds showed a moderate analgesic action but did not exhibit a hypothermic action even at 200mg/kg dose level.