Pea starch increases the dry matter flow at the distal ileum and reduces the amino acids digestibility in ileal digesta collected after 4 hours postprandial of pigs fed low-protein diets

Zhou Junyan,Wang Lu,Yang Guangxin,Yang Lijie,Zeng Xiangfang,Qiao Shiyan 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.7

Objective: The study was aimed to investigate the rules of postprandial changes in intestine digesta dry matter (DM) flow and amino acid digestibility of growing pigs fed low-protein (LP) diets made of different starch. Methods: Eight barrows (28.8±2.1 kg) with a T-cannula at the distal ileum were randomly allotted to an 8×3 Youden square design. Treatments included: waxy corn starch LP (WLP); corn starch LP (CLP) and pea starch LP (PLP). Diets were given at 08:00 and 20:00. Digesta samples were collected in six 2-h stages from 08:00 to 20:00. Results: The Cr concentrations of ileal digesta increased and then decreased in WLP and CLP, while increased continuously in PLP as time passed after postprandial (p<0.05). Higher average Cr concentrations (0.78% and 0.84% vs 0.70%; p<0.05) and lower average DM flow (181.1 g/kg and 166.3 g/kg vs 240.3 g/kg; p<0.001) were observed in WLP and CLP, compared with PLP. The apparent ileal digestibility coefficient of most amino acids in WLP and CLP increased compared with that in PLP. No difference in lysine or methionine digestibility was observed. When digesta were collected in 2-h periods, the apparent ileal digestibility coefficient of amino acids did not change over time. When digesta was collected in 4-h periods from 16:00 to 20:00 and 6-h periods from 14:00 to 20:00 (p<0.05), WLP and CLP showed markedly higher amino acid digestibility than PLP Conclusion: High-amylose slowly digested starch can increase the DM flow at the distal ileum and reduce the apparent ileal digestibility coefficient of amino acids of pigs fed LP diets. Compared with waxy corn starch and corn starch, pea starch reduced the digestibility of amino acids in digesta collected after 4 h postprandial. Objective: The study was aimed to investigate the rules of postprandial changes in intestine digesta dry matter (DM) flow and amino acid digestibility of growing pigs fed low-protein (LP) diets made of different starch.Methods: Eight barrows (28.8±2.1 kg) with a T-cannula at the distal ileum were randomly allotted to an 8×3 Youden square design. Treatments included: waxy corn starch LP (WLP); corn starch LP (CLP) and pea starch LP (PLP). Diets were given at 08:00 and 20:00. Digesta samples were collected in six 2-h stages from 08:00 to 20:00.Results: The Cr concentrations of ileal digesta increased and then decreased in WLP and CLP, while increased continuously in PLP as time passed after postprandial (p<0.05). Higher average Cr concentrations (0.78% and 0.84% vs 0.70%; p<0.05) and lower average DM flow (181.1 g/kg and 166.3 g/kg vs 240.3 g/kg; p<0.001) were observed in WLP and CLP, compared with PLP. The apparent ileal digestibility coefficient of most amino acids in WLP and CLP increased compared with that in PLP. No difference in lysine or methionine digestibility was observed. When digesta were collected in 2-h periods, the apparent ileal digestibility coefficient of amino acids did not change over time. When digesta was collected in 4-h periods from 16:00 to 20:00 and 6-h periods from 14:00 to 20:00 (p<0.05), WLP and CLP showed markedly higher amino acid digestibility than PLPConclusion: High-amylose slowly digested starch can increase the DM flow at the distal ileum and reduce the apparent ileal digestibility coefficient of amino acids of pigs fed LP diets. Compared with waxy corn starch and corn starch, pea starch reduced the digestibility of amino acids in digesta collected after 4 h postprandial.

Bacillus subtilis improves antioxidant capacity and optimizes inflammatory state in broilers

Yu Zhang,Junyan Zhou,Linbao Ji,Lian Zhang,Liying Zhao,Yubing Guo,Haitao Wei,Lin Lu Asian Australasian Association of Animal Productio 2024 Animal Bioscience Vol.37 No.6

Objective: Bacillus subtilis, a kind of probiotic with broad-spectrum antibacterial function, was commonly used in livestock and poultry production. Recent research suggested that Bacillus subtilis may have antioxidant properties and improve immune response. This study aimed to verify the probiotic function of Bacillus subtilis in the production of broiler chickens. Methods: A total of 324 (1-day-old) Arbor Acres broilers were selected and randomly divided into three groups: basal diet group (Ctr Group), basal diet + antibiotic growth promoter group (Ctr + AGP) and basal diet + 0.5% Bacillus subtilis preparation group (Ctr + Bac). The experiment lasted for 42 days. Muscle, serum and liver samples were collected at 42 days for determination. Results: The results showed that Bacillus subtilis could decrease malondialdehyde content in the serum and liver (p<0.05) and increase superoxide dismutase 1 mRNA expression (p<0.01) and total superoxide dismutase (p<0.05) in the liver. In addition, compared with AGP supplementation, Bacillus subtilis supplementation increased interleukin-10 (IL-10) and decreased tumor necrosis factor-α and IL-1β level in the serum (p<0.05). At 45 minutes after slaughter Ctr + Bac presented a higher a^* value of breast muscle than Ctr Group (p<0.05), while significant change in leg muscle was not identified. Moreover, there was no difference in weight, shear force, cooking loss and drip loss of breast and leg muscle between treatments. Conclusion: Our results demonstrate that Bacillus subtilis in diet can enhance antioxidant capacity and optimize immune response of broilers.

Dual oxygen vacancy defects-mediated efficient electron-hole separation via surface engineering of Ag/Bi2MoO6 nanosheets/TiO2 nanobelts ternary heterostructures

Junwei Yin,Zipeng Xing,Junyan Kuang,Zhenzi Li,Qi Zhu,Wei Zhou 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.78 No.-

Surface engineering of Ag/Bi2MoO6 nanosheets/TiO2 nanobelts ternary heterostructures with dualoxygen vacancy defects are successfully synthesized though a hydrothermal-photodeposition methodcombined with high-temperature surface hydrogenation. Bi2MoO6 nanosheets are grown on surface ofTiO2 nanobelts to form heterojunctions, and Ag nanoparticles are decorated on their surfaces for surfaceplasmon resonance (SPR). After surface hydrogenation, oxygen vacancy not only appear on TiO2, but alsoon Bi2MoO6 to achieve dual oxygen vacancy defects for thefirst time. Under visible light irradiation, HAg-BMO/TiO2 shows excellent photodegradation of 93.7% for metribuzin, high photocatalytic H2evolution rate of 143.5 mmol h 1 g 1, and outstanding photoelectrochemical performance.

YTHDF1 promotes the proliferation, migration, and invasion of prostate cancer cells by regulating TRIM44

Li Weijian,Chen Gaohuang,Feng Zhenyu,Zhu Baoyi,Zhou Lilin,Zhang Yuying,Mai Junyan,Jiang Chonghe,Zeng Jianwen 한국유전학회 2021 Genes & Genomics Vol.43 No.12

Background Prostate cancer (PCa) is one of the most common malignancies in men. YTHDF1 may play an important role in promoting PCa progression, but there is no reports to date on YTHDF1 function in PCa. Objective This study explored whether YTHDF1 could regulate TRIM44 in PCa cells. Methods By querying the TCGA database, we evaluated YTHDF1 expression in PCa, the OS and DFS of YTHDF1, and the correlation between YTHDF1 and TRIM44 in PCa. We constructed vectors to interfere with YTHDF1 expression and overexpress TRIM44 to examine the role of YTHDF1 and TRIM44 in PCa cells. Diferentially expressed mRNAs were identifed by mRNA sequencing. The levels of YTHDF1, TRIM44, LGR4, SGTA, DDX20, and FZD8 were measured by qRT-PCR and WB was used to determine YTHDF1 and TRIM44 expression. A CCK-8 assay was used to assess cell proliferation. A Transwell chamber assay was used measure cell migration and invasion ability. Results YTHDF1 was highly expressed in both Pca tissues and cells. PCa patient prognosis with high YTHDF1 expression was relatively poor. Cell function experiments showed that inhibiting YTHDF1 expression decreased cell proliferation, migration, and invasion. RNA sequencing analysis revealed that YTHDF1 may promote PCa cell proliferation, migration, and invasion by modulating TRIM44 expression. Cell function experiments further verifed that YTHDF1 promoted PCa cell proliferation, migration, and invasion by regulating TRIM44. Conclusions YTHDF1 enhances PCa cell proliferation, migration, and invasion by regulating TRIM44.

An Uncertain Graph Method Based on Node Random Response to Preserve Link Privacy of Social Networks

Jun Yan,Jiawang Chen,Yihui Zhou,Zhenqiang Wu,Laifeng Lu 한국인터넷정보학회 2024 KSII Transactions on Internet and Information Syst Vol.18 No.1

In pace with the development of network technology at lightning speed, social networks have been extensively applied in our lives. However, as social networks retain a large number of users’ sensitive information, the openness of this information makes social networks vulnerable to attacks by malicious attackers. To preserve the link privacy of individuals in social networks, an uncertain graph method based on node random response is devised, which satisfies differential privacy while maintaining expected data utility. In this method, to achieve privacy preserving, the random response is applied on nodes to achieve edge modification on an original graph and node differential privacy is introduced to inject uncertainty on the edges. Simultaneously, to keep data utility, a divide and conquer strategy is adopted to decompose the original graph into many sub-graphs and each sub-graph is dealt with separately. In particular, only some larger sub-graphs selected by the exponent mechanism are modified, which further reduces the perturbation to the original graph. The presented method is proven to satisfy differential privacy. The performances of experiments demonstrate that this uncertain graph method can effectively provide a strict privacy guarantee and maintain data utility.

A Differential Privacy Approach to Preserve GWAS Data Sharing based on A Game Theoretic Perspective

Jun Yan,Ziwei Han,Yihui Zhou,Laifeng Lu 한국인터넷정보학회 2022 KSII Transactions on Internet and Information Syst Vol.16 No.3

Genome-wide association studies (GWAS) aim to find the significant genetic variants for common complex disease. However, genotype data has privacy information such as disease status and identity, which make data sharing and research difficult. Differential privacy is widely used in the privacy protection of data sharing. The current differential privacy approach in GWAS pays no attention to raw data but to statistical data, and doesn’t achieve equilibrium between utility and privacy, so that data sharing is hindered and it hampers the development of genomics. To share data more securely, we propose a differential privacy preserving approach of data sharing for GWAS, and achieve the equilibrium between privacy and data utility. Firstly, a reasonable disturbance interval for the genotype is calculated based on the expected utility. Secondly, based on the interval, we get the Nash equilibrium point between utility and privacy. Finally, based on the equilibrium point, the original genotype matrix is perturbed with differential privacy, and the corresponding random genotype matrix is obtained. We theoretically and experimentally show that the method satisfies expected privacy protection and utility. This method provides engineering guidance for protecting GWAS data privacy.