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Zhou, Jia,Yang, Liyang,Wang, Chonglong,Choi, Eui-Sung,Kim, Seon-Won Elsevier 2017 Journal of biotechnology Vol.248 No.-
<P>The 2C-methyl-D-erythritol 4-phosphate (MEP) pathway is a carbon-efficient route for synthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), the building blocks of isoprenoids. However, practical application of a native or recombinant MEP pathway for the mass production of isoprenoids in Escherichia coli has been unsatisfactory. In this study, the entire recombinant MEP pathway was established with plasmids and used for the production of an isoprenoid, protoilludene. E. coli harboring the recombinant MEP pathway plasmid (ME) and a protoilludene synthesis pathway plasmid (AO) produced 10.4 mg/L of protoilludene after 48 h of culture. To determine the rate-limiting gene on plasmid ME, each constituent gene of the MEP pathway was additionally overexpressed on the plasmid AO. The additional overexpression of IPP isomerase (IDI) enhanced protoilludene production to 67.4 mg/L. Overexpression of the Fpr and FIdA protein complex, which could mediate electron transfer from NADPH to Fe-S cluster proteins such as IspG and IspH of the MEP pathway, increased protoilludene production to 318.8 mg/L. Given that it is required for IspC as well as IspG/H, the MEP pathway has high demand for NADPH. To increase the supply of NADPH, a NADH kinase from Saccharomyces cerevisiae (tPos5p) that converts NADH to NADPH was introduced along with the deletion of a promiscuous NADPH-dependent aldehyde reductase (YjgB) that consumes NADPH. This resulted in a protoilludene production of 512.7 mg/L. The results indicate that IDI, Fpr-FIdA redox proteins, and NADPH regenerators are key engineering points for boosting the metabolic flux toward a recombinant MEP pathway. (C) 2017 Elsevier B.V. All rights reserved.</P>
A reliability analysis method for rock slope controlled by weak structural surface
Jia-wen Zhou,Ming-yuan Jiao,Hui-ge Xing,Xing-guo Yang,Yu-chuan Yang 한국지질과학협의회 2017 Geosciences Journal Vol.21 No.3
Catastrophic landslides maybe occur in rock slope due to the effect of strong earthquakes or heavy rainfall. The stability of rock slope is usually controlled by different scales of weak structural surfaces, which are uncertain and randomly exist in the rock slope. According to the geological characteristics of rock slope, two typical failure modes – plane and wedge are possible. A second-order second-moment (SOSM) method is presented to calculate the reliability index and the failure probability of rock slope, which is an improvement over the first-order second-moment (FOSM) method, and performance functions are built up with the classic limit equilibrium method. The presented method is applied to analyze the failure probability of two rock slopes at the Jinping I Hydropower Station and is compared with the Monte Carlo method and the FOSM method. The computed results show that for plane failure, the reliability index and the failure probability determined by the presented method are 0.563 and 28.7%, respectively, and the reliability index and the failure probability determined by Monte Carlo method are 0.677 and 24.9%, respectively. However, for the FOSM method, the reliability index and failure probability are –0.025 and 51.0%, respectively. For both plane failure and wedge failure, the difference between the presented method and the Monte Carlo method is very small, but the failure probability of plane failure determined by FOSM method is larger than that of the other two methods. The presented method can provide a useful tool to evaluate the failure probability of rock slope.
( Jia Wei ),( Xiao Dan Cao ),( Sheng Min Zhou ),( Chao Chen ),( Hai Jun Yu ),( Yao Zhou ),( Ping Wang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.8
Vascular endothelial growth factor (VEGF) plays a key role in angiogenesis through binding to its specific receptors, which mainly occurs to VEGF receptor 2 (VEGFR-2), a kinase insert domain-containing receptor. Therefore, the disruption of VEGFR-2 signaling provides a promising therapeutic approach for the treatment of cancer by inhibiting abnormal or tumorinduced angiogenesis. To explore this potential, we expressed the catalytic domain of VEGFR- 2 (VEGFR-2-CD) as a soluble active kinase in Escherichia coli. The recombinant protein was purified and the VEGFR-2-CD activity was investigated. The obtained VEGFR-2-CD showed autophosphorylation activity and phosphate transfer activity comparable to the commercial enzyme. Furthermore, the IC50 value of known VEGFR-2 inhibitor was determined using the purified VEGFR-2-CD. These results indicated a possibility for functional and economical VEGFR-2-CD expression in E. coli to use for inhibitor screening.
( Jia Zhou ),( Shuangming Yue ),( Benchu Xue ),( Zhisheng Wang ),( Lizhi Wang ),( Quanhui Peng ),( Rui Hu ),( Bai Xue ) 한국축산학회 2022 한국축산학회지 Vol.64 No.1
The reduction of milk yield caused by heat stress in summer is the main condition restricting the economic benefits of dairy farms. To examine the impact of hyperthermia on bovine mammary epithelial (MAC-T) cells, we incubated the MAC-T cells at thermal-neutral (37℃, CON group) and hyperthermic (42℃, HS group) temperatures for 6 h. Subsequently, the cell viability and apoptotic rate of MAC-T cells, apoptosis-related genes expression, casein and amino acid transporter genes, and the expression of the apoptosis-related proteins were examined. Compared with the CON group, hyperthermia significantly decreased the cell viability (p < 0.05) and elevated the apoptotic rate (p < 0.05) of MAC-T cells. Moreover, the expression of heat shock protein (HSP)70, HSP90B1, Bcl-2-associated X protein (BAX), Caspase-9, and Caspase-3 genes was upregulated (p < 0.05). The expression of HSP70 and BAX (pro-apoptotic) proteins was upregulated (p < 0.05) while that of B-cell lymphoma (BCL)2 (antiapoptotic) protein was downregulated (p < 0.05) by hyperthermia. Decreased mRNA expression of mechanistic target of rapamycin (mTOR) signaling pathway-related genes, amino acid transporter genes (SLC7A5, SLC38A3, SLC38A2, and SLC38A9), and casein genes (CSNS1, CSN2, and CSN3) was found in the heat stress (HS) group (p < 0.05) in contrast with the CON group. These findings illustrated that hyperthermia promoted cell apoptosis and reduced the transport of amino acids into cells, which inhibited the milk proteins synthesis in MAC-T cells.
Evolution of Discussion and Discovery in Chinese Engineering Classrooms: A Pilot Study
Jia Zhou,Pei-Luen Patrick Rau,Gavriel Salvendy,Anping Xie 대한사고개발학회 2011 The International Journal of Creativity & Problem Vol.21 No.2
This paper presents the description of a pilot experiment with three classes con ducted in Industrial Engineering classes at Tsinghua University in China (N=125), with the aim of increasing students’ discussions and discovery in the classroom and ultimately increasing students’ engineering creative process. Each class was divided randomly into experimental and control groups. Results indicated that students’ satisfaction increased with some level of discussions in the classroom. But the one semester’s study for the Chinese students did not seem to be sufficient to increase either their generic or engineering specific creativity.
Jia Li,Yan An,Jia-Ning Wang,Xiao-Ping Yin,Huan Zhou,Yong-Sheng Wang 대한생리학회-대한약리학회 2020 The Korean Journal of Physiology & Pharmacology Vol.24 No.5
This study aimed to evaluate the effect of curcumin on brain hypoxicischemic (HI) damage in neonatal rats and whether the phosphoinositide 3-kinase (PI3K)/Akt/vascular endothelial growth factor (VEGF) signaling pathway is involved. Brain HI damage models were established in neonatal rats, which received the following treatments: curcumin by intraperitoneal injection before injury, insulin-like growth factor 1 (IGF-1) by subcutaneous injection after injury, and VEGF by intracerebroventricular injection after injury. This was followed by neurological evaluation, hemodynamic measurements, histopathological assessment, TUNEL assay, flow cytometry, and western blotting to assess the expression of p-PI3K, PI3K, p-Akt, Akt, and VEGF. Compared with rats that underwent sham operation, rats with brain HI damage showed remarkably increased neurological deficits, reduced right blood flow volume, elevated blood viscosity and haematocrit, and aggravated cell damage and apoptosis; these injuries were significantly improved by curcumin pretreatment. Meanwhile, brain HI damage induced the overexpression of p-PI3K, p-Akt, and VEGF, while curcumin pretreatment inhibited the expression of these proteins. In addition, IGF-1 treatment rescued the curcumin-induced down-regulated expression of p- PI3K, p-Akt, and VEGF, and VEGF overexpression counteracted the inhibitory effect of curcumin on brain HI damage. Overall, pretreatment with curcumin protected against brain HI damage by targeting VEGF via the PI3K/Akt signaling pathway in neonatal rats.
Research on detecting moving targets with an improved Kalman filter algorithm
Jia quan Zhou,Wei Wei 한국인터넷정보학회 2023 KSII Transactions on Internet and Information Syst Vol.17 No.9
As science and technology evolve, object detection of moving objects has been widely used in the context of machine learning and artificial intelligence. Traditional moving object detection algorithms, however, are characterized by relatively poor real-time performance and low accuracy in detecting moving objects. To tackle this issue, this manuscript proposes a modified Kalman filter algorithm, which aims to expand the equations of the system with the Taylor series first, ignoring the higher order terms of the second order and above, when the nonlinear system is close to the linear form, then it uses standard Kalman filter algorithms to measure the situation of the system. which can not only detect moving objects accurately but also has better real-time performance and can be employed to predict the trajectory of moving objects. Meanwhile, the accuracy and real-time performance of the algorithm were experimentally verified.