EXPLORING STORYTELLING ELEMENTS IN MARKETING

Yun-Chia Tang,Li-Wei Wu 글로벌지식마케팅경영학회 2018 Global Marketing Conference Vol.2018 No.07

The present research is seeking to enhance their customers’ brand attitudes, especially in terms of which type of products should be their focus for customers with varying storytelling elements. Storytelling in marketing is critical for business, because it is a cornerstone for building strong relationship in the modern world. Storytelling elements also influence consumers’ emotional responses to brand. Besides, organization of information in an advertising narrative might influence the consumer's choice of an appropriate information-processing strategy (Mattila 2000), the narrative message of a story will affect consumer’s preference for a brand, thereby affects consumer’s purchase intention. However, despite some elements have been discussed in previous studies related to narratives (Chatman 1978; Papadatos 2006), we do not understand exactly why or when customers respond to stories. Therefore, to examine the storytelling effectives of different types of reversal factors, this research investigates the influence of different types of stories on brand attitudes in marketing. In sum, by studying the elements of reversal stories, this expected outcomes of this research intends to provide researchers and practitioners implications: 1. design a successful brand story can enhance corporate image and attract more consumers. 2. In practice, marketers need to combine storytelling factors with different products, and manipulate them with different weights to design the optimal advertisements or marketing campaign. 3. This study would be aroused the interesting to investigate other story elements and how these elements to affect brand attitudes.

Morphology and transcriptome differences between the haploid and diploid drones of Apis cerana

Wei-Yu Yan,Hai-Yan Gan,Shu-Yun Li,Jing-Hua Hu,ZilongWang,Xiaobo Wu,Zhi Jiang Zeng 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.4

In general, drone honey bees are haploid and develop from unfertilized eggs. However, a diploid drone can arise in an inbred colony. In this study, the morphological characteristics and gene expression profile of the haploid and diploid drones of Apis cerana were analyzed to reveal the differences between them. The ploidy level of the droneswas identified by FlowCytometry (FCM). The characters of the forewings,wetweight of reproductive organs and of newly emerged drones, were investigated. Then, a high throughput transcriptomic analysis was performed using RNA-seq in diploid and haploid drones. The results showed that the wet weight and reproductive organs of diploid droneswere significantly lighter than those of haploid drones. About 201 million high-quality reads were generated from RNA-seq, and 75.99–78.12% of the data weremapped to Apis cerana genome. 360 genes were differentially expressed between diploid and haploid drone, with 152 up-regulated and 208 downregulated in the diploid drones. Functional analysis identified that these genes were significantly enriched in 28 pathways. Comparative transcriptomic analysis detected several differentially expressed genes, which lay a foundation for future studies on molecular mechanisms underlying biology difference in drones in Apis cerana.

A 60GHz Wireless Cooperative Communication System Based on Switching Beamforming

( Wei Shi ),( Jingjing Wang ),( Yun Liu ),( Qiuna Niu ),( Hao Zhang ),( Chunlei Wu ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.4

The challenge of penetrating obstacles along with impact from weak multipath effects makes 60GHz signal very difficult to be transmitted in non-line of sight (NLOS) channel. So 60GHz system is vulnerable to obstructions and thus likely results in link interruption. While the application of cooperative technology to solve link blockage problemin 60GHz system should consider the characteristic of directional transmission for 60GHz signal. Therefore in this paper a system is proposed to solve the link blockage problem in 60GHz NLOS communication environment based on the concept of cooperation and also the beamforming technology, which is the basis of directional transmission for 60GHz communication system. The process of anti-blockage solution with cooperative communication is presented in detail, and the fast switching and recovery schemes are well designed. The theoretical values of symbol error rate (SER) using decode and forward (DF) cooperation and amplify and forward (AF) cooperation are presented respectively when the common channel interference exists. Simulation results demonstrate that the performance based on DF cooperation is better than the performance based on AF cooperation when directional transmission is used.

Biocontrol Traits and Antagonistic Potential of Bacillus amyloliquefaciens Strain NJZJSB3 Against Sclerotinia sclerotiorum, a Causal Agent of Canola Stem Rot

( Yun Cheng Wu ),( Jun Yuan ),( Waseem Raza ),( Qi Rong Shen ),( Qi Wei Huang ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.10

Bacillus amyloliquefaciens strain NJZJSB3 has shown antagonism of several phytopathogens in vitro, especially Sclerotinia sclerotiorum. Both the broth culture and cell suspension of strain NJZJSB3 could completely protect the detached leaves of canola (Brassica napus) from S. sclerotiorum infection. In pot experiments, the application of strain NJZJSB3 cell suspension (108 CFU/ml) decreased the disease incidence by 83.3%, a result similar to commercially available fungicide (Dimetachlone). In order to investigate the potential biocontrol mechanisms of strain NJZJSB3, the nonvolatile antifungal compounds it produces were identified as iturin homologs using HPLC-ESI-MS. Antifungal volatile organic compounds were identified by gas chromatography-mass spectrometry. The detected volatiles toluene, phenol, and benzothiazole showed antifungal effects against S. sclerotiorum in chemical control experiments. Strain NJZJSB3 also produced biofilm, siderophores and cell-wall-degrading enzymes (protease and β-1,3-glucanase). These results suggest that strain NJZJSB3 can be a tremendous potential agent for the biological control of sclerotinia stem rot.

Downregulation of Human DAB2IP Gene Expression in Renal Cell Carcinoma Results in Resistance to Ionizing Radiation

Yun, Eun-Jin,Lin, Chun-Jung,Dang, Andrew,Hernandez, Elizabeth,Guo, Jiaming,Chen, Wei-Min,Allison, Joyce,Kim, Nathan,Kapur, Payal,Brugarolas, James,Wu, Kaijie,He, Dalin,Lai, Chih-Ho,Lin, Ho,Saha, Debab American Association for Cancer Research 2019 Clinical Cancer Research Vol.25 No.14

<P><B>Purpose:</B></P><P>Renal cell carcinoma (RCC) is known to be highly radioresistant but the mechanisms associated with radioresistance have remained elusive. We found DOC-2/DAB2 interactive protein (DAB2IP) frequently downregulated in RCC, is associated with radioresistance. In this study, we investigated the underlying mechanism regulating radioresistance by DAB2IP and developed appropriate treatment.</P><P><B>Experimental Design:</B></P><P>Several RCC lines with or without DAB2IP expression were irradiated with ionizing radiation (IR) for determining their radiosensitivities based on colony formation assay. To investigate the underlying regulatory mechanism of DAB2IP, immunoprecipitation-mass spectrometry was performed to identify DAB2IP-interactive proteins. PARP-1 expression and enzymatic activity were determined using qRT-PCR, Western blot analysis, and ELISA. <I>In vivo</I> ubiquitination assay was used to test PARP-1 degradation. Furthermore, <I>in vivo</I> mice xenograft model and patient-derived xenograft (PDX) model were used to determine the effect of combination therapy to sensitizing tumors to IR.</P><P><B>Results:</B></P><P>We notice that DAB2IP-deficient RCC cells acquire IR-resistance. Mechanistically, DAB2IP can form a complex with PARP-1 and E3 ligases that is responsible for degrading PARP-1. Indeed, elevated PARP-1 levels are associated with the IR resistance in RCC cells. Furthermore, PARP-1 inhibitor can enhance the IR response of either RCC xenograft model or PDX model.</P><P><B>Conclusions:</B></P><P>In this study, we unveil that loss of DAB2IP resulted in elevated PARP-1 protein is associated with IR-resistance in RCC. These results provide a new targeting strategy to improve the efficacy of radiotherapy of RCC.</P>

Low temperature fabrication of (001) FePt on amorphous substrates

Yun-Chang Wu,Liang-Wei Wang,Chih-Huang Lai 한국자기학회 2007 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

ZNF552, a novel human KRAB/C2H2 zinc finger protein, inhibits AP-1- and SRE-mediated transcriptional activity

( Yun Deng ),( Bi Sheng Liu ),( Xiong Wei Fan ),( Yue Qun Wang ),( Ming Tang ),( Xiao Yang Mo ),( Yong Qing Li ),( Zao Chu Ying ),( Yong Qi Wan ),( Na Luo ),( Jun Mei Zhou ),( Xiu Shan Wu ),( Wu Zhou 한국생화학분자생물학회 (구 한국생화학회) 2010 BMB Reports Vol.43 No.3

In this study, we report the identification and characterization of a novel C2H2 zinc finger protein, ZNF552, from a human embryonic heart cDNA library. ZNF552 is composed of three exons and two introns and maps to chromosome 19q13.43. The cDNA of ZNF552 is 2.3 kb, encoding 407 amino acids with an amino-terminal KRAB domain and seven carboxyl-terminal C2H2 zinc finger motifs in the nucleus and cytoplasm. Northern blotting analysis indicated that a 2.3 kb transcript specific for ZNF552 was expressed in liver, lung, spleen, testis and kidney, especially with a higher level in the lung and testis in human adult tissues. Reporter gene assays showed that ZNF552 was a transcriptional repressor, and overexpression of ZNF552 in the COS-7 cells inhibited the transcriptional activities of AP-1 and SRE, which could be relieved through RNAi analysis. Deletion studies showed that the KRAB domain of ZNF552 may be involved in this inhibition. [BMB reports 2010; 43(3): 193-198]

Synergistic efficacy of LBH and αB-crystallin through inhibiting transcriptional activities of p53 and p21

( Yun Deng ),( Yong Qing Li ),( Xiong Wei Fan ),( Wu Zhou Yuan ),( Hua Ping Xie ),( Xiao Yang Mo ),( Yan Yan ),( Jun Mei Zhou ),( Yue Qun Wang ),( Xian Li Ye ),( Yong Qi Wan ),( Xiu Shan Wu ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.6

LBH is a transcription factor as a candidate gene for CHD associated with partial trisomy 2p syndrome. To identify potential LBH-interacting partners, a yeast two-hybrid screen using LBH as a bait was performed with a human heart cDNA library. One of the clones identified encodes αB-crystallin. Co-immunoprecipitation and GST pull-down assays showed that LBH interacts with αB-crystallin, which is further confirmed by mammalian two-hybrid assays. Co-localization analysis showed that in COS-7 cells, αB-crystallin that is cytoplasmic alone, accumulates partialy in the nucleus when co-transfected with LBH. Transient transfection assays indicated that overexpression of LBH or αB-crystallin reduced the transcriptional activities of p53 and p21, respectively, Overexpression of both αB-crystallin and LBH together resulted in a stronger repression of the transcriptional activities of p21 and p53. These results showed that the interaction of LBH and αB-crystallin may inhibit synergistically the transcriptional regulation of p53 and p21. [BMB reports 2010; 43(6): 432-437]

Genome-wide analysis of codon usage bias patterns in an enterotoxigenic Escherichia coli F18 strain

Ri Wei Xia,Wen Bin Bao,Xue Mei Yin,Wei Yun Qin,Guo Qiang Zhu,Sheng Long Wu 한국유전학회 2017 Genes & Genomics Vol.39 No.11

Enterogenic Escherichia coli (ETEC) F18 strains are the main pathogenic bacteria causing severe diarrhea in humans and domestic animals. However, the information about synonymous codon usage pattern of ETEC F18 genome remains unclear. We conducted a genome-wide analysis of synonymous codon usage patterns in the ETEC F18 strain SRA: SAMN02471895. After filtering of the complete genome sequence, 4327 coding sequences were analyzed using multivariate statistical methods to calculate synonymous codon usage patterns and to evaluate the influence of various factors in shaping the codon usage. The mean GC content was 51.38%, with a slight preference for G/C-ending codons. Twenty-two codons were determined as ‘‘optimal codons”. ENC plots showed some of the genes were on or close to the expected curve, while only points with low-ENC values were below the curve. PR2 analysis showed that GC and AT were not used proportionally, suggesting major roles for mutational pressure and natural selection in shaping usage. Neutrality plots showed a significant correlation between GC12 and GC3, suggesting that mutational pressure is responsible for nucleotide composition in shaping the strength of codon usage. Translational selection was the main factor shaping the codon usage pattern of ETEC F18 genome, while other factors such as protein length, GRAVY and ARO values also influenced codon usage to some extent. We analyzed the codon usage pattern systematically and identified the factors shaping codon usage bias in the ETEC F18 genome. Such information further elucidates the mechanisms of synonymous codon usage bias and provides the basis of molecular genetic engineering and evolutionary studies.

Effect of Lactobacillus rhamnosus hsryfm 1301 on the Gut Microbiota and Lipid Metabolism in Rats Fed a High-Fat Diet

( Da Wei Chen ),( Zhen Quan Yang ),( Xia Chen ),( Yu Jun Huang ),( Bo Xing Yin ),( Fei Xiang Guo ),( Hai Qing Zhao ),( Jia Di Huang ),( Yun Wu ),( Rui Xia Gu ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.5

Accumulating evidence indicates that lactic acid bacteria could improve host physiology and lipid metabolism. To investigate the effect of the gut microbiota on host lipid metabolism, a hyperlipidemic rat model was established by feeding rats a high-fat diet for 28 days, and the gut microbiota of the rats was analyzed using real-time PCR before and after administration of Lactobacillus rhamnosus hsryfm 1301 and its fermented milk for 28 days. The findings showed that the Lactobacillus spp., Bifidobacterium spp., Bacteroides spp., and Enterococcus spp. content in the hyperlipidemic rats gut was increased significantly (p < 0.05), while the Clostridium leptum and Enterobacter spp. content was decreased significantly after intervening with L. rhamnosus hrsyfm 1301 and its fermented milk for 28 days (p < 0.05). Furthermore, the lipid levels of the serum and the liver were decreased significantly (p < 0.05) and the fecal water content was increased significantly (p < 0.05) in the hyperlipidemic rats after the intervention, and hepatocyte fatty degeneration of liver tissues was also prevented. A positive correlation was observed between the Clostridium leptum content and the level of serum cholesterol, triglycerides, low-density lipoprotein, and high-density lipoprotein, and a negative correlation was observed between the Enterobacter spp. content and the Lactobacillus spp. and Bifidobacterium spp. content in the hyperlipidemic rats gut. These results suggest that the gut microbiota and lipid metabolism of hyperlipidemic rats could be improved by supplementation with L. rhamnosus hsryfm 1301 and its fermented milk.