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Characterization of a novel variant HMW‐glutenin gene from Elymus canadensis
Qian‐Tao Jiang,Yu‐Ming Wei,Tao Liu,Ji‐Rui Wang,Zhi‐En Pu,Xiu‐Jin Lan,You‐Liang Zheng,Zhen‐Xiang Lu 한국유전학회 2010 Genes & Genomics Vol.32 No.4
High molecular weight (HMW) glutenin subunits (GS) play a key role in the determination of end‐use quality of wheat and other cereal crops. In this study, we report the isolation and characterization of both promoter region and ORF of novel HMW‐GS allele 1St1.3 from a perennial Triticeae species,Elymus canadensis. The amino acid (AA) sequences of E. canadensis 1St1.3 were deduced as 434 aa. Its protein primary structure comprises a signal peptide with a conserved N‐terminal domain, a central repetitive domain and a C‐terminal domain. E. canadensis 1St 1.3 possesses several distinct characteristics which are different from those of wheat HMW‐GSs. The N‐terminal domains of E. canadensis 1St 1.3 resemble that of y‐type subunits, while their C‐terminal domains are more similar to x‐type subunits. The deletion of 85 bp fragment has been observed in promoter region of 1St 1.3, however which has not interrupted the expression of this gene. Our results indicate that 1St 1.3 is novel HMW‐GS variants which will be valuable for enhancing our understanding of structural differentiation and the evolutionary relationship among HMW‐GSs in Triticeae species.
Qian, Zhi‐,Gang,Xia, Xiao‐,Xia,Lee, Sang Yup Wiley Subscription Services, Inc., A Wiley Company 2011 Biotechnology and bioengineering Vol.108 No.1
<P><B>Abstract</B></P><P>A five carbon linear chain diamine, cadaverine (1,5‐diaminopentane), is an important platform chemical having many applications in chemical industry. Bio‐based production of cadaverine from renewable feedstock is a promising and sustainable alternative to the petroleum‐based chemical synthesis. Here, we report development of a metabolically engineered strain of <I>Escherichia coli</I> that overproduces cadaverine in glucose mineral salts medium. First, cadaverine degradation and utilization pathways were inactivated. Next, <SMALL>L</SMALL>‐lysine decarboxylase, which converts <SMALL>L</SMALL>‐lysine directly to cadaverine, was amplified by plasmid‐based overexpression of the <I>cadA</I> gene under the strong <I>tac</I> promoter. Furthermore, the <SMALL>L</SMALL>‐lysine biosynthetic pool was increased by the overexpression of the <I>dapA</I> gene encoding dihydrodipicolinate synthase through the replacement of the native promoter with the strong <I>trc</I> promoter in the genome. The final engineered strain was able to produce 9.61 g L<SUP>−1</SUP> of cadaverine with a productivity of 0.32 g L<SUP>−1</SUP> h<SUP>−1</SUP> by fed‐batch cultivation. The strategy reported here should be useful for the bio‐based production of cadaverine from renewable resources. Biotechnol. Bioeng. 2011; 108:93–103. © 2010 Wiley Periodicals, Inc.</P>
Clinical significance and prognostic value of C-reactive protein/albumin ratio in gastric cancer
Qian Yu,Ke-zhi Li,Yan-jun Fu,Yanping Tang,Xin-qiang Liang,Zhi-qing Liang,Ji-hong Bai 대한외과학회 2021 Annals of Surgical Treatment and Research(ASRT) Vol.100 No.6
Purpose: This study was aimed to evaluate the clinical significance and prognostic value of CRP/albumin ratio (CAR) in patients with gastric cancer. Methods: The data of 205 gastric cancer patients who underwent surgery was analyzed retrospectively. The association of CAR with the clinical features and prognostic value in gastric cancer was analyzed. The data of this study was combined with previous studies to further determine the prognostic value of CAR in patients with gastric cancer using a metaanalysis method. Results: Cox analysis revealed that preoperative CAR was an independent prognosis indicator in patients with gastric cancer. High expression of CAR indicated a shorter survival time than in those with lower expression. CAR has a higher prognostic value in the 1-, 3-, and 5-year overall survival in patients with gastric cancer. CAR showed significant difference regarding the gastric cancer patients’ age, M stage, and clinical stage. The discriminate value of CAR in M stage of gastric cancer was high (area under the curve, 0.809). A meta-analysis combining previous data and our data showed that preoperative CAR demonstrated a significant association with the overall survival of patients with gastric cancer. Conclusion: This study demonstrated that preoperative CAR could serve as an important prognostic indicator in patients with gastric cancer.
Qian-mei Jiang,Shuai Yu,Xiaofeng Dong,Huai-shun Wang,Jie Hou,Zhi-chao Huang,Zhi-liang Guo,Shou-jiang You,Guo-dong Xiao 대한신경과학회 2022 Journal of Clinical Neurology Vol.18 No.3
Background and Purpose This study aimed to construct an optimal dynamic nomogram for predicting malignant brain edema (MBE) in acute ischemic stroke (AIS) patients after endovascular thrombectomy (ET). Methods We enrolled AIS patients after ET from May 2017 to April 2021. MBE was defined as a midline shift of >5 mm at the septum pellucidum or pineal gland based on follow-up computed tomography within 5 days after ET. Multivariate logistic regression and LASSO (least absolute shrinkage and selection operator) regression were used to construct the nomogram. The area under the receiver operating characteristic curve (AUC) and decisioncurve analysis were used to compare our nomogram with two previous risk models for predicting brain edema after ET. Results MBE developed in 72 (21.9%) of the 329 eligible patients. Our dynamic web-based nomogram (https://successful.shinyapps.io/DynNomapp/) consisted of five parameters: basal cistern effacement, postoperative National Institutes of Health Stroke Scale (NIHSS) score, brain atrophy, hypoattenuation area, and stroke etiology. The nomogram showed good discrimination ability, with a C-index (Harrell’s concordance index) of 0.925 (95% confidence interval=0.890–0.961), and good calibration (Hosmer-Lemeshow test, p=0.386). All variables had variance inflation factors of <1.5 and tolerances of >0.7, suggesting no significant collinearity among them. The AUC of our nomogram (0.925) was superior to those of Xiang-liang Chen and colleagues (0.843) and Ming-yang Du and colleagues (0.728). Conclusions Our web-based dynamic nomogram reliably predicted the risk of MBE in AIS patients after ET, and hence is worthy of further evaluation.
SEMI-DISCRETE CENTRAL DIFFERENCE METHOD FOR DETERMINING SURFACE HEAT FLUX OF IHCP
Qian, Zhi,Fu, Chu-Li Korean Mathematical Society 2007 대한수학회지 Vol.44 No.6
We consider an inverse heat conduction problem(IHCP) in a quarter plane which appears in some applied subjects. We want to determine the heat flux on the surface of a body from a measured temperature history at a fixed location inside the body. This is a severely ill-posed problem in the sense that arbitrarily "small" differences in the input temperature data may lead to arbitrarily "large" differences in the surface flux. A semi-discrete central difference scheme in time is employed to deal with the ill posed problem. We obtain some error estimates which also give the information about how to choose the step length in time. Some numerical examples illustrate the effects of the proposed method.
Metabolic engineering of Escherichia coli for the production of putrescine: A four carbon diamine
Qian, Zhi-Gang,Xia, Xiao-Xia,Lee, Sang Yup Wiley Subscription Services, Inc., A Wiley Company 2009 Biotechnology and bioengineering Vol.104 No.4
<P>A four carbon linear chain diamine, putrescine (1,4-diaminobutane), is an important platform chemical having a wide range of applications in chemical industry. Biotechnological production of putrescine from renewable feedstock is a promising alternative to the chemical synthesis that originates from non-renewable petroleum. Here we report development of a metabolically engineered strain of Escherichia coli that produces putrescine at high titer in glucose mineral salts medium. First, a base strain was constructed by inactivating the putrescine degradation and utilization pathways, and deleting the ornithine carbamoyltransferase chain I gene argI to make more precursors available for putrescine synthesis. Next, ornithine decarboxylase, which converts ornithine to putrescine, was amplified by a combination of plasmid-based and chromosome-based overexpression of the coding genes under the strong tac or trc promoter. Furthermore, the ornithine biosynthetic genes (argC-E) were overexpressed from the trc promoter, which replaced the native promoter in the genome, to increase the ornithine pool. Finally, strain performance was further improved by the deletion of the stress responsive RNA polymerase sigma factor RpoS, a well-known global transcription regulator that controls the expression of ca. 10% of the E. coli genes. The final engineered E. coli strain was able to produce 1.68 g L<SUP>−1</SUP> of putrescine with a yield of 0.168 g g<SUP>−1</SUP> glucose. Furthermore, high cell density cultivation allowed production of 24.2 g L<SUP>−1</SUP> of putrescine with a productivity of 0.75 g L<SUP>−1</SUP> h<SUP>−1</SUP>. The strategy reported here should be useful for the bio-based production of putrescine from renewable resources, and also for the development of strains capable of producing other diamines, which are important as nitrogen-containing platform chemicals. Biotechnol. Bioeng. 2009; 104: 651–662 © 2009 Wiley Periodicals, Inc.</P>
A Novel Mutant of Human Papillomavirus Type 18 E6E7 Fusion Gene and its Transforming Activity
Zhou, Zhi-Xiang,Zhao, Chen,Li, Qian-Qian,Zeng, Yi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17
Background: Persistent human papillomavirus (HPV) infection, especially with high-risk types such as HPV16 and HPV18, has been identified as the primary cause of cervical cancer. E6 and E7 are the major onco-proteins of high-risk HPVs, which are consistently expressed in HPV infected tissues but absent in normal tissues and represent ideal therapeutic targets for immunotherapy of cervical cancer. Materials and Methods: In this study, the optimized fusion gene HPV18 E6E7 (HPV18 ofE6E7) was constructed according to genetic codon usage for human genes. At the same time, for safety future clinical application, a mutant of HPV18 ofE6E7 fusion gene was generated by site-directed mutagenesis at L52G for the E6 protein and C98G for the E7 protein. Results: HPV18-E6E7 mutant (HPV18 ofmE6E7) constructed in this work not only lost the transformation capability for NIH 3T3 cells and tumorigenicity in BALB/c nude mice, but also maintained very good stability and antigenicity. Conclusion: These results suggest that the mutant should undergo further study for application as a safe antigenspecific therapeutic vaccine for HPV18-associated tumors.