A vibration-based approach for detecting arch dam damage using RBF neural networks and Jaya algorithms

Zahoor Hussain,Ali Zar,Muhammad Akbar,Bassam A. Tayeh,Zhibin Lin 국제구조공학회 2023 Smart Structures and Systems, An International Jou Vol.32 No.5

The study presents a new hybrid data-driven method by combining radial basis functions neural networks (RBFNN) with the Jaya algorithm (JA) to provide effective structural health monitoring of arch dams. The novelty of this approach lies in that only one user-defined parameter is required and thus can increase its effectiveness and efficiency, as compared to other machine learning techniques that often require processing a large amount of training and testing model parameters and hyper-parameters, with high time-consuming. This approach seeks rapid damage detection in arch dams under dynamic conditions, to prevent potential disasters, by utilizing the RBF-NNN to seamlessly integrate the dynamic elastic modulus (DEM) and modal parameters (such as natural frequency and mode shape) as damage indicators. To determine the dynamic characteristics of the arch dam, the JA sequentially optimizes an objective function rooted in vibration-based data sets. Two case studies of hyperbolic concrete arch dams were carefully designed using finite element simulation to demonstrate the effectiveness of the RBF-NN model, in conjunction with the Jaya algorithm. The testing results demonstrated that the proposed methods could exhibit significant computational time-savings, while effectively detecting damage in arch dam structures with complex nonlinearities. Furthermore, despite training data contaminated with a high level of noise, the RBF-NN and JA fusion remained the robustness, with high accuracy.

In vitro Study on the Antimicrobial Activity of Human Tears with Respect to Age

Zahoor, Muhammad,Bahadar, Haji,Ayaz, Muhammad,Khan, Ajmal,Shah, Muhammad Jalat Korean Society for Clinical Laboratory Science 2018 대한임상검사과학회지(KJCLS) Vol.50 No.2

Lysozyme is present in tears and has the ability to inhibit bacterial growth. In addition, it acts as a physiological scavenger for harmful substances. In the present study, sixteen tear samples from people of different ages were evaluated for their antibacterial spectrum against selected bacterial strains (Escherichia coli, Shigella sonnei, Staphylococcus aureus, Salmonella enterica Typhi). A radial diffusion assay was used to evaluate the antibacterial potential of tear samples. To correlate the antibacterial activities of these tear samples, the concentration of lysozyme in the tear samples was also determined. Ampicillin was used as a standard drug. The zone of inhibition (mm) was used to measure the antibacterial property of the tears. All samples showed good antibacterial activities. The tear samples of children showed antibacterial activities in the range of 4.40~5.00 mm inhibition zones against the selected bacterial strains. The tear samples from the young and adults showed good antibacterial potential with a zone of inhibition in the range of 3.20~4.00 and 4.00~5.50 mm, respectively. The tear samples from the old age group showed inhibition zones from 1.50~5 mm. The adult tear samples showed the maximum inhibition against the selected bacterial strains among all groups. The lysozyme concentration was 1.7 mg/mL, 1.95 mg/mL, 2.13 mg/mL, and 1.76 mg/mL for children, young, adults, and elderly, respectively. In conclusion, the tears from adults have the high inhibition potential. In addition, this data also showed that the lysozyme contents in the tear sample increased with age until 40~42 years.

Indirubin-3--Oxime Reverses Bone Loss in Ovariectomized and Hindlimb-Unloaded Mice Via Activation of the Wnt/-Catenin Signaling

Zahoor, M.,Cha, P. H.,Min, D. S.,Choi, K. Y. American Society for Bone and Mineral Research 2014 Journal of bone and mineral research Vol.29 No.5

Effect of crosslinker feed content on catalaytic activity of silver nanoparticles fabricated in multiresponsive microgels

Zahoor Hussain Farooqi,Shanza Rauf Khan,Tajamal Hussain,Robina Begum,Kiran Ejaz,Shumaila Majeed,Muhammad Ajmal,Farah Kanwal,Mohammad Siddiq 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.9

We investigated the effect of crosslinking density of poly(N-isopropyl acrylamide-co-acrylic acid) micro-gels on catalytic activity of silver nanoparticles fabricated hybrid microgels. Multiresponsive poly(N-isopropyl acryla-mide-co-acrylic acid) microgels with 2, 4, 6 and 8 mole percentage of N,N-methylene-bis-acrylamide were synthesizedby emulsion polymerization. These microgels were characterized by dynamic light scattering and were used as mi-croreactors to synthesize silver nanoparticles. Hybrid system was characterized by ultraviolet-visible spectroscopy. Thecatalytic activity of hybrid microgels with different crosslinker content was compared by studying the reduction of p-nitrophenol as a model reaction. Kinetics of reaction was monitored by spectrophotometry. The value of the apparentrate constant decreases from 0.568 to 0.313 min−1, when content of crosslinker are increased from 2 to 8 mole percent-age respectively. This decreases in value of apparent rate constant is due to increase in diffusional barrier offered byhigh crosslinking of polymer network at high mole percentages of N,N-methylene-bis-acrylamide.

Exploring the Catalytic Significant Residues of Serine Protease Using Substrate-Enriched Residues and a Peptidase Inhibitor

( Zahoor Khan ),( Maryam Shafique ),( Amir Zeb ),( Nusrat Jabeen ),( Sehar Afshan Naz ),( Arif Zubair ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 한국미생물·생명공학회지 Vol.49 No.1

Serine proteases are the most versatile proteolytic enzymes with tremendous applications in various industrial processes. This study was designed to investigate the biochemical properties, critical residues, and the catalytic potential of alkaline serine protease using in-silico approaches. The primary sequence was analyzed using ProtParam, SignalP, and Phyre2 tools to investigate biochemical properties, signal peptide, and secondary structure, respectively. The three-dimensional structure of the enzyme was modeled using the MODELLER program present in Discovery Studio followed by Molecular Dynamics simulation using GROMACS 5.0.7 package with CHARMM36m force field. The proteolytic potential was measured by performing docking with casein- and keratin-enriched residues, while the effect of the inhibitor was studied using phenylmethylsulfonyl fluoride, (PMSF) applying GOLDv5.2.2. Molecular weight, instability index, aliphatic index, and isoelectric point for serine protease were 39.53 kDa, 27.79, 82.20 and 8.91, respectively. The best model was selected based on the lowest MOLPDF score (1382.82) and DOPE score (-29984.07). The analysis using ProSA-web revealed a Z-score of -9.7, whereas 88.86% of the residues occupied the most favored region in the Ramachandran plot. Ser327, Asp138, Asn261, and Thr326 were found as critical residues involved in ligand binding and execution of biocatalysis. Our findings suggest that bioengineering of these critical residues may enhance the catalytic potential of this enzyme.

Synthesis of Polypyrrole Nano/Microspheres Using Cobalt(III) as an Oxidizing Agent and Its Ammonia Sensing Behavior

Zahoor Ahmad,Muhammad Aziz Choudhary,Aneela Mehmood,Riffat Wakeel,Tashfeen Akhtar,Muhammad Aftab Rafiq 한국고분자학회 2016 Macromolecular Research Vol.24 No.7

Polypyrrole (ppy) has been synthesized by employing [Co(NH3)6]Cl3 as a new oxidizing agent under basic condition in aquous solution. The optimized ratio of monomer to oxidizing agent has been taken as 1:2. The reaction was allowed to continue for 24-48 h at room temperature. The as synthesized ppy displayed well defined morphology, which was seemed as time dependent. It was nanospheres at 24 h, micro/nano mixture at 36 h and microspheres and microfibers at 48 h. The FTIR confirmed the formation of ppy due to the presence of characteristics broader ring and N-H stretching vibrations. The XRD showed the development of crystallites only within the fibrous ppy. The thermal and structural correlation was determined using TGA and DSC techniques alongwith SEM. The polymer was found to be considerably more stable as compared with similarly synthesized using other such oxidants. The conductivity of pure polymer and effect of NH3 over its conductivity was analyzed by four probe technique. Its strong potential of NH3 sensing has been confirmed from this technique due to showing linear increase in its conductivity. These results demonstrated that cobalt(III) is a potential oxidizing agent to produce thermally stable nano/microspheres of ppy. Moreover, the material was found quite useful for its characteristics of NH3 sensing that can be applied for other nitrogenous bases.

Personal Verification using Fingerprint Texture Feature

Zahoor Ahmad Jhat,Ajaz Hussain Mir,Seemin Rubab 보안공학연구지원센터 2011 International Journal of Security and Its Applicat Vol.5 No.3

Novel Synthesis of Mannosamine Conjugated Magnetic Nanoparticles for Purification and Stabilization of Human Lysosomal β-mannosidase

Zahoor Qadir Samra,Muhammad Amin Athar 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.5

Abstract Human β-mannosidase (MANB) was purified to homogeneity directly from lysosomes by using mannosamine conjugated magnetic (Fe₃O₄) nanoparticles, DE-52 cellulose, and sephadex G-200 chromatography. Fe₃O₄nanoparticles were synthesized and utilized ammonia to attach the amino group on the nanoparticles. The particles were covalently attached with Dmannosamine by cross linker glutaraldehyde and confirmed by FTIR spectroscopy. In FTIR analysis, the peaks appeared at 2,356.6 cm−¹ for −N = CH linkage and at 3,378.4 cm−1, 3,664.9 cm−¹ for −OH groups confirmed the conjugation of Dmannosamine with Fe₃O₄ nanoparticles. Results showed a single band of 97 kDa of purified MANB in SDS-PAGE. The isoelectric point was 4.5 and the Km and Vã~ñ values were 2.51 mM and 0.315 μM/min/mg, respectively. The purification fold was 329 with 68% yield. The optimal activity was at pH 5.0 and 75% activity was stable in 20% glycerol at 4oC. The enzyme activity was inhibited by Ni²+, Zn²+, Cd²+, Cu²+, Mo²+, Ag+¹, iodoacetate, SDS, DMF, DMSO, ethanol, and acetone; slightly reduced by Pb²+, Co²+, EDTA, DTT, and β-mercaptoethanol. The activity was not affected by Mg²+, Mn²+, Sn²+, Ca²+, Fe³+, PMSF, Triton X-100, D-mannosamine, D-mannose, D-mannitol, D-glucose, and D-fructose. The homogeneity of MANB enzyme was further confirmed by 2D-PAGE and immunoblot. This is the first novel report of conjugation of Dmannosamine with Fe₃O₄nanoparticles for purification of human MANB enzyme Abstract Human β-mannosidase (MANB) was purified to homogeneity directly from lysosomes by using mannosamine conjugated magnetic (Fe₃O₄) nanoparticles, DE-52 cellulose, and sephadex G-200 chromatography. Fe₃O₄nanoparticles were synthesized and utilized ammonia to attach the amino group on the nanoparticles. The particles were covalently attached with Dmannosamine by cross linker glutaraldehyde and confirmed by FTIR spectroscopy. In FTIR analysis, the peaks appeared at 2,356.6 cm−¹ for −N = CH linkage and at 3,378.4 cm−1, 3,664.9 cm−¹ for −OH groups confirmed the conjugation of Dmannosamine with Fe₃O₄ nanoparticles. Results showed a single band of 97 kDa of purified MANB in SDS-PAGE. The isoelectric point was 4.5 and the Km and Vã~ñ values were 2.51 mM and 0.315 μM/min/mg, respectively. The purification fold was 329 with 68% yield. The optimal activity was at pH 5.0 and 75% activity was stable in 20% glycerol at 4oC. The enzyme activity was inhibited by Ni²+, Zn²+, Cd²+, Cu²+, Mo²+, Ag+¹, iodoacetate, SDS, DMF, DMSO, ethanol, and acetone; slightly reduced by Pb²+, Co²+, EDTA, DTT, and β-mercaptoethanol. The activity was not affected by Mg²+, Mn²+, Sn²+, Ca²+, Fe³+, PMSF, Triton X-100, D-mannosamine, D-mannose, D-mannitol, D-glucose, and D-fructose. The homogeneity of MANB enzyme was further confirmed by 2D-PAGE and immunoblot. This is the first novel report of conjugation of Dmannosamine with Fe₃O₄nanoparticles for purification of human MANB enzyme

Carbon/titanium oxide supported bimetallic platinum/iridium nanocomposites as bifunctional electrocatalysts for lithium-air batteries

Zahoor, A.,Christy, M.,Kim, Y.,Arul, A.,Lee, Y. S.,Nahm, K. S. Springer Science + Business Media 2016 Journal of solid state electrochemistry Vol.20 No.5

<P>Platinum (Pt) and iridium (Ir) catalysts are well known to strongly enhance the oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) kinetics, respectively. Pt-Ir-based bimetallic compounds along with carbon-supported titanium oxides (C-TiO2) have been synthesized for the application as electrocatalysts in lithium oxygen batteries. Transition metal oxide-based bimetallic nanocomposites (Pt-Ir/C-TiO2) were prepared by an incipient wetness impregnation technique. The as-prepared electrocatalysts were composed of a well-dispersed homogenous alloy of nanoparticles as confirmed by X-ray diffraction patterns and Fourier transform scanning electron microscopy analyses. The electrochemical characterizations reveal that the Pt-Ir/C-TiO2 electrocatalysts were bifunctional with high activity for both ORR and OER. When applied as an air cathode catalyst in lithium-air batteries, the electrocatalyst improved the battery performance in terms of capacity, reversibility, and cycle life compared to that of cathodes without any catalysts.</P>

Soil Phosphorus Retention Capacity of Different Amendments

Zahoor Ahmad 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6