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      저자 : Zahoor Qadir Samra (검색결과 1 건)
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        Novel Synthesis of Mannosamine Conjugated Magnetic Nanoparticles for Purification and Stabilization of Human Lysosomal β-mannosidase

        Zahoor Qadir Samra,Muhammad Amin Athar 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.5

        Abstract Human β-mannosidase (MANB) was purified to homogeneity directly from lysosomes by using mannosamine conjugated magnetic (Fe₃O₄) nanoparticles, DE-52 cellulose, and sephadex G-200 chromatography. Fe₃O₄nanoparticles were synthesized and utilized ammonia to attach the amino group on the nanoparticles. The particles were covalently attached with Dmannosamine by cross linker glutaraldehyde and confirmed by FTIR spectroscopy. In FTIR analysis, the peaks appeared at 2,356.6 cm−¹ for −N = CH linkage and at 3,378.4 cm−1, 3,664.9 cm−¹ for −OH groups confirmed the conjugation of Dmannosamine with Fe₃O₄ nanoparticles. Results showed a single band of 97 kDa of purified MANB in SDS-PAGE. The isoelectric point was 4.5 and the Km and Vã~ñ values were 2.51 mM and 0.315 μM/min/mg, respectively. The purification fold was 329 with 68% yield. The optimal activity was at pH 5.0 and 75% activity was stable in 20% glycerol at 4oC. The enzyme activity was inhibited by Ni²+, Zn²+, Cd²+, Cu²+, Mo²+, Ag+¹, iodoacetate, SDS, DMF, DMSO, ethanol, and acetone; slightly reduced by Pb²+, Co²+, EDTA, DTT, and β-mercaptoethanol. The activity was not affected by Mg²+, Mn²+, Sn²+, Ca²+, Fe³+, PMSF, Triton X-100, D-mannosamine, D-mannose, D-mannitol, D-glucose, and D-fructose. The homogeneity of MANB enzyme was further confirmed by 2D-PAGE and immunoblot. This is the first novel report of conjugation of Dmannosamine with Fe₃O₄nanoparticles for purification of human MANB enzyme Abstract Human β-mannosidase (MANB) was purified to homogeneity directly from lysosomes by using mannosamine conjugated magnetic (Fe₃O₄) nanoparticles, DE-52 cellulose, and sephadex G-200 chromatography. Fe₃O₄nanoparticles were synthesized and utilized ammonia to attach the amino group on the nanoparticles. The particles were covalently attached with Dmannosamine by cross linker glutaraldehyde and confirmed by FTIR spectroscopy. In FTIR analysis, the peaks appeared at 2,356.6 cm−¹ for −N = CH linkage and at 3,378.4 cm−1, 3,664.9 cm−¹ for −OH groups confirmed the conjugation of Dmannosamine with Fe₃O₄ nanoparticles. Results showed a single band of 97 kDa of purified MANB in SDS-PAGE. The isoelectric point was 4.5 and the Km and Vã~ñ values were 2.51 mM and 0.315 μM/min/mg, respectively. The purification fold was 329 with 68% yield. The optimal activity was at pH 5.0 and 75% activity was stable in 20% glycerol at 4oC. The enzyme activity was inhibited by Ni²+, Zn²+, Cd²+, Cu²+, Mo²+, Ag+¹, iodoacetate, SDS, DMF, DMSO, ethanol, and acetone; slightly reduced by Pb²+, Co²+, EDTA, DTT, and β-mercaptoethanol. The activity was not affected by Mg²+, Mn²+, Sn²+, Ca²+, Fe³+, PMSF, Triton X-100, D-mannosamine, D-mannose, D-mannitol, D-glucose, and D-fructose. The homogeneity of MANB enzyme was further confirmed by 2D-PAGE and immunoblot. This is the first novel report of conjugation of Dmannosamine with Fe₃O₄nanoparticles for purification of human MANB enzyme

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