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Yum. Seungshic,Suh, Ji-Yoeun,Woo, Seonock,Jeon, Hyung-Wook,Kim, Keun Chong,Suh, Dong Sang 한국유전학회 2004 Genes & Genomics Vol.26 No.3
This study analyzed the D-loop region of 28 Korean cattle (Bos taurus) from 7 different localities to examine the genetic diversity of Korean cattle populations. Approximately 1.6 kb of D-loop region sequences was determined and nucleotide variations were compared using the sequences of the 28 Korean cattle and the published sequences of 18 Japanese, 10 European, 6 African and 6 Indian cattle. Dendrograms indicated that the Korean cattle were more closely related to the European than Japanese cattle. The Indian cattle showed the furthest genetic relation to the Korean cattle. This paper discussed the genetic relationship of Korean domestic cattle compared with European, African, Indian and Japanese cattle based on the sequence variations of the mitochondria) DNA D-loop region.
우선옥,Seungshic Yum,윤문근,Sa Heung Kim,Jongrak Lee,Jeong Ha Kim,Taek-Kyun Lee 한국통합생물학회 2005 Animal cells and systems Vol.9 No.4
A rapid, simple and efficient method to extract RNA from the adult polyps of a soft coral, marine cnidarian, Scleronephthya gracillimum (Kkenthal), was developed in this study. The highest yield and purity of RNA was obtained with the lysis solution containing 35 mM EDTA, 0.7 M LiCl, 7.0% SDS, and 200 mM Tris-Cl (pH 9.0). Approximately 40 g of total RNA was extracted from 200 mg of liquid nitrogen-pulverized polyp tissue. The ratio of absorbance at 260 nm and 280 nm ranged from 1.8 to 2.0. The results of the reverse transcription polymerase chain reaction (RT-PCR) with -actin gene specific primers and Northern blot analysis using the same gene probe revealed that the RNA extracted by our method had high quality, and was sufficient for subsequent molecular biological analyses. This method was effective for RNA extraction from other soft coral species which belong to the genus Dendronephthya.
Impact of zinc oxide nanoparticles on the bacterial community of Hydra magnipapillata
Ade Yamindago,Nayun Lee,Seonock Woo,Seungshic Yum 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.1
Backgrounds Zinc oxide nanoparticles (ZnO NPs) are extensively used for various products. In this study, the effects of ZnO NPs exposure in diversity and community composition of the bacteria associated with H. magnipapillata were investigated. This study provides insight into possible impacts of ZnO NPs on aquatic organisms. Methods 454-pyrosequencing analysis of the bacterial 16S rRNA gene was applied to H. magnipapillata after exposure to 10 mg/L ZnO NPs (Ø 20 nm). Results Acute exposure to ZnO NPs changed the diversity and compositions of the associated bacteria. The composition of Curvibacter decreased, but Flectobacillus and Delftia increased; these two genera are known to have beneficial functions. Conclusion The changes in diversity and composition of the associated bacteria may indicate the possible mechanisms by which the associated bacteria maintain their mutual interactions and support the health of their host after exposure to ZnO NPs.
Woo, Seonock,Won, Hyokyoung,Lee, Aekyung,Yum, Seungshic 대한독성유전단백체학회 2012 Molecular & cellular toxicology Vol.10 No.2
Transcriptional changes in the expression of stress-related genes (catalase, glutathione peroxidase, glutathione S-transferase, glutathione reductase, metallothionein, and ubiquitin) in intestinal, liver and muscle tissues of Javanese medaka (Oryzias javanicus) exposed to $17{\beta}$-estradiol (E2) were investigated using real-time quantitative PCR. The lipid peroxidation levels and superoxide dismutase activity in liver tissues was also examined at various exposure concentrations. In fish exposed to 10, 100 and 1000 ${\mu}g/L$ E2 the level of catalase mRNA increased significantly in intestinal and muscle tissues, while expression of the glutathione peroxidase gene showed the opposite trend in liver and muscle tissues. Expression of the glutathione S-transferase gene increased in a dose-dependent manner in liver and muscle tissues of fish exposed to E2, relative to the non-exposed control group. The level of glutathione reductase mRNA increased markedly in the liver tissues of all fish exposed to 100 and 1000 ${\mu}g/L$ E2, but decreased in intestinal tissues. The metallothionein gene was strongly downregulated in intestinal and liver tissues of fish exposed to E2, but slightly upregulated in muscle tissues. In all fish exposed to 100 and 1000 ${\mu}g/L$ E2 expression of the ubiquitin gene increased markedly in liver and muscle tissue, but not in intestinal tissues. Lipid peroxidation and superoxide dismutase activities increased significantly in all $17{\beta}$-estradiol treatment groups, and were correlated to the exposure concentration. Thus, E2 exposure differentially affected the transcription of a range of stress-related genes in various tissues of Javanese medaka, suggesting that analysis of transcriptional changes in these genes could be used as a rapid assay of the effects of E2 exposure.
Karyotypes of Two Ischnochiton Species : Ischnochitonidae: Polyplacophora
Choe, Byung Lae,Rho, Boon Jo,Song, Jun-Im,Yum, Seungshic 이화여자대학교 생명과학연구소 1995 생명과학연구논문집 Vol.6 No.-
연두군부(Ischnochiton comptus)와 가는줄연두군부(I. hakodadensis) 두 종의 정소를 재료로 하여 핵형 분석을 실시하였다. 두 종의 염색체는 모두 중부염색체로 구성되어 있고, 염색체 수는 n=12이며, 성염색체는 관찰되지 않았다. 이들 염색체는 이들의 크기로는 별도의 염색체군으로 나누어지지 않았다. Chromosomes prepared from the male gonads of the two polyplacophoran species. Ischnochiton comptus and I. hakodadensis were analysed. The numbers of chromosomes of the two species were 24 in diploid and 12 in haploid. In the case of I. comptus. this chromosome count coincides with the report of Nishikawa and Ishida(1969). The sex-dete-mining chromosomes have not been detected. All of the chfomosomes were classified of metacintric and could not be grouped into different classes according to their lengths.
Jong Sung Ahn,오윤식,김정하,우선옥,Jung Hyun Oak,Seungshic Yum 한국조류학회I 2004 ALGAE Vol.19 No.2
A more rapid and efficient method to extract RNA from Ecklonia cava Kjellman (Laminariales, Phaeophyceae) was introduced in this study. Each step of the procedure was evaluated and the optimal concentration of each chemical in the lysis solution was determined. Tissue pulverization with PVPP and β-mercaptoethanol in the lysis solution were not essential for RNA extraction of this species. The highest yield and purity of E. cava RNA were obtained by the lysis solution containing 1% CTAB, 1 M NaCl, 0.7% PVP, 10 mM EDTA and 100 mM Tris-Cl (pH 9.0). Approximately 8 μg of RNA was obtained from 200 mg of ground tissue. The ratios of the absorbance at 260 nm and 280 nm were from 1.6 to 1.8 and those of at 230 nm and 260 nm were from 1.8 to 2.0. The extracted RNAs obtained in this study turned out to have a sufficient quality for cDNA synthesis.