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Relaxation effect of "SUNALPHA"™
Nobuyukiaoi,Kobayashi, Kanan,Nagato, Yukiko,Jueja, Lekh Raj,Kim, Mujo 한국정신과학학회 1997 한국정신과학회 학술대회 논문집 Vol.6 No.-
"SUNALPHA" is a formulation of green tea components(mainlytheanineand poiyphenols), ther amino acids and vitamins. Theanine is a unique amino acid and is found only in green tea which gives specific umami tatste Recently, It has been reported that theanine has various biological functions. for example, it acts as an antagonist of caffeine in this study, we determind the relaxation effect of "SUNALPHA" on human volunteers with low anxiety levels by determining the genration of α-waves(a brain wave, 8~13Hz), which is a bio-marker of the relaxed state in human, "SUNALPHA" was administered orally to human volunteers (aged 18 to 22) at the dose of 450 mg in 100ml of water. The mental condition of volunteers was estimated by monitoring the emission of brain waves in a closed room with 40 lux of luminous intensity, at 25℃. Volunteers were administered either a blank (water)or a test solution of "SUNALPHA". THEN, emission OF α-brian waves was measured for one hour by attaching electrodes to their heads. In the topographic mapping, it could be observed that α-wares were dominant in theocciptal area and spreaded toward the frontal area only in those volunteers who were given the test solubon of "SUNALPHA". Most of the volunteers, 75%, showed the same tendency due to an in crease of the emission of the α-abrain waves. From this results, it can be suggested that "SUNALPHA" promotes the generation of α-waves in the brian, giving asense of relaxation to the boody. As the industnal production of "SUNALPHA"has been achieved, this attractive formulation could be an excellent food additive to make streseed people relax by generating α-brain waves.
preethi devanand,Yukiko Oya,santhoshkumarsundaramoorthy,송계용,Tatsuro Watanabe,Yasuhito Kobayashi,Yoshihiko Shimizu,홍순억,Masami Suganuma,임인경 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-
To understand the regulation of Helicobacter pylori (H. pylori)-associated gastric carcinogenesis, we examined the effect of B-cell translocation gene 2 (BTG2) expression on the biological activity of Tipα, an oncoprotein secreted from H. pylori. BTG2, the human ortholog of mouse TIS21 (BTG2/TIS21), has been reported to be a primary response gene that is transiently expressed in response to various stimulations. Here, we report that BTG2 is constitutively expressed in the mucous epithelium and parietal cells of the gastric gland in the stomach. Expression was increased in the mucous epithelium following H. pylori infection in contrast to its loss in human gastric adenocarcinoma. Indeed, adenoviral transduction of BTG2/TIS21 significantly inhibited Tipα activity in MKN-1 and MGT-40, human and mouse gastric cancer cells, respectively, thereby downregulating tumor necrosis factor-α (TNFα) expression and Erk1/2 phosphorylation by reducing expression of nucleolin, a Tipα receptor. Chromatin immunoprecipitation proved that BTG2/TIS21 inhibited Sp1 expression and its binding to the promoter of the nucleolin gene. In addition, BTG2/TIS21 expression significantly reduced membrane-localized nucleolin expression in cancer cells, and the loss of BTG2/TIS21 expression induced cytoplasmic nucleolin availability in gastric cancer tissues, as evidenced by immunoblotting and immunohistochemistry. Higher expression of BTG2 and lower expression of nucleolin were accompanied with better overall survival of poorly differentiated gastric cancer patients. This is the first report showing that BTG2/TIS21 inhibits nucleolin expression via Sp1 binding, which might be associated with the inhibition of H. pylori-induced carcinogenesis. We suggest that BTG2/TIS21 is a potential inhibitor of nucleolin in the cytoplasm, leading to inhibition of carcinogenesis after H. pylori infection.
Azusa Sasaki,Yasushi Nakamura,Yukiko Kobayashi,Wataru Aoi,Takako Nakamura,Koji Shirota,Noboru Suetome,Michiaki Fukui,Tomoaki Matsuo,Shigehisa Okamoto,Yuri Tashiro,Eun Y. Park,Kenji Sato 한국식품연구원 2018 Journal of Ethnic Foods Vol.5 No.1
Background The fruit of Katsura-uri, traditionally used in the preparation of pickles in Japan, is facing an extinction crisis. In addition to the traditional dishes prepared from Katsura-uri, alternative dishes using the fruit should be devised to increase consumer demands for the protection of the heirloom vegetable. We attempted designing new Katsura-uri contemporary dishes and assessed the application of Katsura-uri juice as a functional drink without raising blood glucose levels. Methods Cooking experiments were conducted with Katsura-uri in its ripening stages, based on the advice from a licensed chef and a registered dietitian in Japan. In the questionnaire-based sensory evaluation, consumer acceptability of Katsura-uri juice was assessed. The blood glucose levels were measured after healthy volunteers consumed the juice. Results We demonstrated six new Katsura-uri dishes. In the questionnaire-based sensory evaluation of Katsura-uri juice, the assessment values for taste and fragrance were high. In human trials, the levels of incremental area under the curve and glucose spike were significantly lower after consumption of Katsura-uri juice, as compared to those after consumption of muskmelon juice. Conclusion Katsura-uri-containing contemporary dishes and juice would help continue the consumption of the vegetable. Based on the results of the questionnaire, we also concluded that the use of Katsura-uri as a functional drink without raising blood glucose levels is superior to its use as contemporary dishes. These findings provide useful strategies to protect Katsura-uri from extinction.
Angiogenesis in newly regenerated bone by secretomes of human mesenchymal stem cells
Katagiri, Wataru,Kawai, Takamasa,Osugi, Masashi,Sugimura-Wakayama, Yukiko,Sakaguchi, Kohei,Kojima, Taku,Kobayashi, Tadaharu Korean Association of Maxillofacial Plastic and Re 2017 Maxillofacial Plastic Reconstructive Surgery Vol.39 No.-
Background: For an effective bone graft for reconstruction of the maxillofacial region, an adequate vascular network will be required to supply blood, osteoprogenitor cells, and growth factors. We previously reported that the secretomes of bone marrow-derived mesenchymal stem cells (MSC-CM) contain numerous growth factors such as insulin-like growth factor (IGF)-1, transforming growth factor $(TGF)-{\beta}1$, and vascular endothelial growth factor (VEGF), which can affect the cellular characteristics and behavior of regenerating bone cells. We hypothesized that angiogenesis is an important step for bone regeneration, and VEGF is one of the crucial factors in MSC-CM that would enhance its osteogenic potential. In the present study, we focused on VEGF in MSC-CM and evaluated the angiogenic and osteogenic potentials of MSC-CM for bone regeneration. Methods: Cytokines in MSC-CM were measured by enzyme-linked immunosorbent assay (ELISA). Human umbilical vein endothelial cells (HUVECs) were cultured with MSC-CM or MSC-CM with anti-VEGF antibody (MSC-CM + anti-VEGF) for neutralization, and tube formation was evaluated. For the evaluation of bone and blood vessel formation with micro-computed tomography (micro-CT) and for the histological and immunohistochemical analyses, a rat calvarial bone defect model was used. Results: The concentrations of IGF-1, VEGF, and $TGF-{\beta}1$ in MSC-CM were $1515.6{\pm}211.8pg/mL$, $465.8{\pm}108.8pg/mL$, and $339.8{\pm}14.4pg/mL$, respectively. Tube formation of HUVECs, bone formation, and blood vessel formation were increased in the MSC-CM group but decreased in the MSC-CM + anti-VEGF group. Histological findings suggested that new bone formation in the entire defect was observed in the MSC-CM group although it was decreased in the MSC-CM + anti-VEGF group. Immunohistochemistry indicated that angiogenesis and migration of endogenous stem cells were much more abundant in the MSC-CM group than in the MSC-CM + anti-VEGF group. Conclusions: VEGF is considered a crucial factor in MSC-CM, and MSC-CM is proposed to be an adequate therapeutic agent for bone regeneration with angiogenesis.